(Circular) (Six-base) MAPSORT of: pFN21ASDA2003.seq Check: 9990 from: 1 to: 6040 pFN21ASDA2003 6040 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2300 2300 Size: 6040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4103 5487 5755 1632 Size: 2471 1384 268 1917 Fragments arranged by size: 2471 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3783 5355 3783 Size: 1572 4468 ApaLI G'TGCA_C Cuts at: 3265 4097 5253 3265 Size: 832 1156 4052 Fragments arranged by size: 4052 1156 832 ApoI r'AATT_y Cuts at: 1493 3211 3321 3349 3575 3727 5836 1493 Size: 1718 110 28 226 152 2109 1697 Fragments arranged by size: 2109 1718 1697 226 152 110 28 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 3365 1870 Size: 73 1422 4545 Fragments arranged by size: 4545 1422 73 BamHI G'GATC_C Cuts at: 2912 3370 3737 2912 Size: 458 367 5215 Fragments arranged by size: 5215 458 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3361 4816 618 Size: 325 217 627 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3359 1391 Size: 1968 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2261 928 Size: 971 362 4707 Fragments arranged by size: 4707 971 362 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4651 136 Size: 107 122 71 631 605 2979 1525 Fragments arranged by size: 2979 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2060 3269 4101 5257 1790 Size: 270 1209 832 1156 2573 Fragments arranged by size: 2573 1209 1156 832 270 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2367 2751 2835 Size: 416 53 83 399 24 950 384 84 Cuts at: 2835 3087 3255 3771 4729 442 Size: 252 168 516 958 1753 Fragments arranged by size: 1753 958 950 516 416 399 384 252 168 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2595 3015 3115 3283 4700 1239 Size: 935 421 420 100 168 1417 2579 Fragments arranged by size: 2579 1417 935 421 420 168 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2114 4703 882 Size: 617 615 2589 2219 Fragments arranged by size: 2589 2219 617 615 BsaAI yAC'GTr Cuts at: 493 1204 3337 3831 493 Size: 711 2133 494 2702 Fragments arranged by size: 2702 2133 711 494 BsaBI GATnn'nnATC Cuts at: 2187 6034 2187 Size: 3847 2193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 3847 4229 4864 Size: 53 83 186 936 512 1802 382 635 Cuts at: 4864 275 Size: 1451 Fragments arranged by size: 1802 1451 936 635 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2643 4473 4871 5145 5292 5703 2643 Size: 1830 398 274 147 411 2980 Fragments arranged by size: 2980 1830 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5815 Fragments arranged by size: 5815 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6040 BseYI C'CCAG_C Cuts at: 5243 5739 5243 Size: 496 5544 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2245 2781 2782 2866 2950 3033 3034 Size: 573 509 536 1 84 84 83 1 Cuts at: 3034 3118 3202 1163 Size: 84 84 4001 Fragments arranged by size: 4001 573 536 509 84 84 84 84 83 1 1 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3269 4101 4186 5257 3269 Size: 832 85 1071 4052 Fragments arranged by size: 4052 1071 832 85 BsmI GAATG_Cn' Cuts at: 2444 3551 3644 2444 Size: 1107 93 4840 Fragments arranged by size: 4840 1107 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 3269 3359 4101 4186 5257 1391 Size: 399 270 1209 90 742 85 1071 2174 Fragments arranged by size: 2174 1209 1071 742 399 270 90 85 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 2671 3929 5659 2671 Size: 1258 1730 3052 Fragments arranged by size: 3052 1730 1258 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2267 2300 3395 844 Size: 1423 33 1095 3489 Fragments arranged by size: 3489 1423 1095 33 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2236 4535 4709 65 Size: 1183 988 2299 174 1396 Fragments arranged by size: 2299 1396 1183 988 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 2007 4844 2007 Size: 2837 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2912 3370 3737 4122 4139 5591 5677 5689 Size: 940 458 367 385 17 1452 86 12 Cuts at: 5689 6035 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 940 458 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4531 Fragments arranged by size: 4531 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2518 2655 2770 2854 3106 3190 3556 4350 4370 Size: 137 115 84 252 84 366 794 20 Cuts at: 4370 2518 Size: 4188 Fragments arranged by size: 4188 794 366 252 137 115 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2079 2319 Size: 54 1425 50 275 18 201 48 240 Cuts at: 2319 3487 4376 6005 8 Size: 1168 889 1629 43 Fragments arranged by size: 1629 1425 1168 889 275 240 201 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3970 1353 Size: 2617 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2595 3015 3115 3283 4103 4700 Size: 393 542 421 420 100 168 820 597 Cuts at: 4700 5487 5755 1239 Size: 787 268 1524 Fragments arranged by size: 1524 820 787 597 542 421 420 393 268 168 100 EcoNI CCTnn'n_nnAGG Cuts at: 2117 2117 Size: 6040 EcoRI G'AATT_C Cuts at: 3321 3349 3727 3321 Size: 28 378 5634 Fragments arranged by size: 5634 378 28 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2035 2067 4681 4713 4755 Size: 32 135 32 463 32 2614 32 42 Cuts at: 4755 4787 1373 Size: 32 2626 Fragments arranged by size: 2626 2614 463 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 10 Size: 54 1425 343 489 3729 Fragments arranged by size: 3729 1425 489 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2676 2758 3998 4357 4516 518 Size: 648 1510 82 1240 359 159 2042 Fragments arranged by size: 2042 1510 1240 648 359 159 82 MspA1I CmG'CkG Cuts at: 1606 2030 2451 3456 4133 5281 5526 1606 Size: 424 421 1005 677 1148 245 2120 Fragments arranged by size: 2120 1148 1005 677 424 421 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4531 Fragments arranged by size: 4531 957 552 NdeI CA'TA_TG Cuts at: 387 2507 2755 387 Size: 2120 248 3672 Fragments arranged by size: 3672 2120 248 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3398 4943 1132 Size: 97 2169 1545 2229 Fragments arranged by size: 2229 2169 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6040 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3009 3610 3009 Size: 601 5439 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2473 2505 2473 Size: 32 6008 PstI C_TGCA'G Cuts at: 830 2297 2662 3392 830 Size: 1467 365 730 3478 Fragments arranged by size: 3478 1467 730 365 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2293 2490 2542 2658 3124 3388 4523 Size: 219 1248 197 52 116 466 264 1135 Cuts at: 4523 5204 5395 826 Size: 681 191 1471 Fragments arranged by size: 1471 1248 1135 681 466 264 219 197 191 116 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3337 3831 493 Size: 2844 494 2702 Fragments arranged by size: 2844 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 2836 2920 3004 3088 1517 Size: 1319 84 84 84 4469 Fragments arranged by size: 4469 1319 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2022 2206 3479 513 Size: 552 957 184 1273 3074 Fragments arranged by size: 3074 1273 957 552 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4067 4084 4237 4422 4867 325 Size: 1255 2487 17 153 185 445 1498 Fragments arranged by size: 2487 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4286 5998 96 Size: 274 80 33 51 1443 2309 1712 138 Fragments arranged by size: 2309 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2897 2929 6000 6032 2897 Size: 32 3071 32 2905 Fragments arranged by size: 3071 2905 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 2179 3376 2179 Size: 1197 4843 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 2312 4169 2312 Size: 1857 4183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI