Restriction map for
pFN21ASDA1994
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII AclI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BfrI BglI BlnI
BsaAI BsaBI BsiWI BsmBI BsoBI
BspDI BspHI BspLU11I BsrBI BssHII
BssSI BstBI Bsu36I ClaI CpoI
DraIII DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FseI FspI HindIII
KasI MamI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 133 GGTACC G^GTACC
AccI 1601 GTATAC GT^[AC][GT]AC
AccII 27 CGCG CG^CG
AccIII 2005 TCCGGA T^CCGGA
AcyI 139 GGCGTC G[GA]^CG[CT]C
Aor51HI 52 AGCGCT AGC^GCT
Asp718I 133 GGTACC G^GTACC
BbvCI 589 CCTCAGC CCTCAGC(-5/-2)
BclI 629 TGATCA T^GATCA
BcnI 72 CCCGG CC^[GC]GG
BsaHI 139 GGCGTC G[GA]^CG[CT]C
BsaI 523 GAGACC GGTCTC(1/5)
BsaWI 2005 TCCGGA [AT]^CCGG[AT]
BseAI 2005 TCCGGA T^CCGGA
BsiEI 29 CGGCCG CG[GA][CT]^CG
Bsp1407I 1968 TGTACA T^GTACA
BspEI 2005 TCCGGA T^CCGGA
BsrFI 136 ACCGGC [GA]^CCGG[CT]
BsrGI 1968 TGTACA T^GTACA
Bst1107I 1601 GTATAC GTA^TAC
BstEII 703 GGTCACC G^GT[ACGT]ACC
BstPI 703 GGTCACC G^GT[ACGT]ACC
BstUI 27 CGCG CG^CG
BstXI 2130 CCAGCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1601 GTATAC GTA^TAC
Cfr10I 136 ACCGGC [GA]^CCGG[CT]
DraI 1255 TTTAAA TTT^AAA
DraII 3011 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 26 CCGCGG C^C[GA][CT]GG
EagI 29 CGGCCG C^GGCCG
EclXI 29 CGGCCG C^GGCCG
Eco47III 52 AGCGCT AGC^GCT
Eco52I 29 CGGCCG C^GGCCG
EcoNI 261 CCTTTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3011 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 703 GGTCACC G^GT[ACGT]ACC
FauI 3181 CCCGC CCCGC(4/6)
FbaI 629 TGATCA T^GATCA
HgaI 140 GCGTC GACGC(5/10)
Hin1I 139 GGCGTC G[GA]^CG[CT]C
KpnI 133 GGTACC GGTAC^C
KspI 26 CCGCGG CCGC^GG
MaeII 220 ACGT A^CGT
MroI 2005 TCCGGA T^CCGGA
MvnI 27 CGCG CG^CG
NciI 72 CCCGG CC^[GC]GG
NotI 28 GCGGCCGC GC^GGCCGC
PflMI 1551 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3011 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 2910 GAGCTC GAGCT^C
SacII 26 CCGCGG CCGC^GG
SbfI 2113 CCTGCAGG CCTGCA^GG
ScaI 889 AGTACT AGT^ACT
Sse8387I 2113 CCTGCAGG CCTGCA^GG
SspBI 1968 TGTACA T^GTACA
Van91I 1551 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 775 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 2156 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2840 CAGCCTCTG
Asp700I 2435 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2528 GAATGCCTTC
BbsI 3081 GAAGAC GAAGAC(2/6)
1682 GTCTTC
BciVI 1727 GTATCC GTATCC(6/5)
2072 GTATCC
BlpI 271 GCTCAGC GC^T[ACGT]AGC
2059 GCTCAGC
Bpu1102I 271 GCTCAGC GC^T[ACGT]AGC
2059 GCTCAGC
BpuAI 3081 GAAGAC GAAGAC(2/6)
1682 GTCTTC
BsmFI 225 GGGAC GGGAC(10/14)
2101 GTCCC
BsmI 639 GAATGC GAATGC(1/-1)
2528 GAATGC
CelII 271 GCTCAGC GC^T[ACGT]AGC
2059 GCTCAGC
EarI 447 CTCTTC CTCTTC(1/4)
520 GAAGAG
EcoT14I 996 CCTTGG C^C[AT][AT]GG
1536 CCAAGG
HaeII 52 AGCGCT [GA]GCGC^[CT]
109 AGCGCC
Ksp632I 447 CTCTTC CTCTTC(1/4)
520 GAAGAG
MfeI 2359 CAATTG C^AATTG
2714 CAATTG
MunI 2359 CAATTG C^AATTG
2714 CAATTG
SspI 1024 AATATT AAT^ATT
2191 AATATT
StyI 996 CCTTGG C^C[AT][AT]GG
1536 CCAAGG
XmnI 2435 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2528 GAATGCCTTC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanI
BanII BbvI BfaI BglII BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII