(Circular) (Six-base) MAPSORT of: pFN21ASDA1994.seq Check: 4905 from: 1 to: 7948 pFN21ASDA1994 7948 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2887 4330 2887 Size: 1443 6505 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5758 278 Size: 53 83 186 936 4222 2468 Fragments arranged by size: 4222 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3625 5291 1937 Size: 1688 1666 4594 Fragments arranged by size: 4594 1688 1666 Acc65I G'GTAC_C Cuts at: 2156 5269 2156 Size: 3113 4835 AclI AA'CG_TT Cuts at: 1563 6075 6448 1563 Size: 4512 373 3063 Fragments arranged by size: 4512 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 3692 3771 3966 4260 5123 6011 7395 Size: 566 1494 79 195 294 863 888 1384 Cuts at: 7395 7663 1632 Size: 268 1917 Fragments arranged by size: 1917 1494 1384 888 863 566 294 268 195 79 AfeI AGC'GCT Cuts at: 2077 2077 Size: 7948 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7751 AflIII A'CryG_T Cuts at: 1203 6847 7824 1203 Size: 5644 977 1327 Fragments arranged by size: 5644 1327 977 AgeI A'CCGG_T Cuts at: 6779 6779 Size: 7948 AhdI GACnn_n'nnGTC Cuts at: 6677 6677 Size: 7948 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7948 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4494 1184 Size: 3310 4638 AlwNI CAG_nnn'CTG Cuts at: 4184 4868 5691 7263 4184 Size: 684 823 1572 4869 Fragments arranged by size: 4869 1572 823 684 ApaLI G'TGCA_C Cuts at: 6005 7161 6005 Size: 1156 6792 ApoI r'AATT_y Cuts at: 1493 2935 4669 5071 5257 5483 5635 7744 1493 Size: 1442 1734 402 186 226 152 2109 1697 Fragments arranged by size: 2109 1734 1697 1442 402 226 186 152 AseI AT'TA_AT Cuts at: 160 1032 6502 160 Size: 872 5470 1606 Fragments arranged by size: 5470 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7948 AvaI C'yCGr_G Cuts at: 1870 1943 5273 1870 Size: 73 3330 4545 Fragments arranged by size: 4545 3330 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2148 2181 2148 Size: 33 7915 BamHI G'GATC_C Cuts at: 5278 5645 5278 Size: 367 7581 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2709 3883 5189 5269 Size: 325 217 627 369 553 1174 1306 80 Cuts at: 5269 6724 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1306 1174 627 553 369 325 217 80 BanII G_rGCy'C Cuts at: 1391 2526 3276 3496 4779 4937 5228 5267 1391 Size: 1135 750 220 1283 158 291 39 4072 Fragments arranged by size: 4072 1283 1135 750 291 220 158 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3698 5111 928 Size: 971 1799 1413 3765 Fragments arranged by size: 3765 1799 1413 971 BbvCI CC'TCA_GC Cuts at: 2613 2613 Size: 7948 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6139 6173 1903 Size: 34 4202 34 3678 Fragments arranged by size: 4202 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4106 5840 7050 3761 Size: 345 1734 1210 4659 Fragments arranged by size: 4659 1734 1210 345 BclI T'GATC_A Cuts at: 1548 2652 1548 Size: 1104 6844 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6559 136 Size: 107 122 71 631 605 4887 1525 Fragments arranged by size: 4887 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2382 2464 3651 7943 2382 Size: 82 1187 4292 2387 Fragments arranged by size: 4292 2387 1187 82 BlpI GC'TnA_GC Cuts at: 2295 4083 5365 2295 Size: 1788 1282 4878 Fragments arranged by size: 4878 1788 1282 Bme1580I G_kGCm'C Cuts at: 1790 2712 3886 5192 6009 7165 1790 Size: 922 1174 1306 817 1156 2573 Fragments arranged by size: 2573 1306 1174 1156 922 817 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7948 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2786 3198 4032 Size: 416 53 83 399 24 1369 412 834 Cuts at: 4032 4341 4702 5128 5679 6637 442 Size: 309 361 426 551 958 1753 Fragments arranged by size: 1753 1369 958 834 551 426 416 412 399 361 309 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7948 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3657 4954 3657 Size: 1297 6651 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3102 3843 5102 6608 1239 Size: 1863 741 1259 1506 2579 Fragments arranged by size: 2579 1863 1506 1259 741 Bpu10I CC'TnA_GC Cuts at: 2613 4731 5121 5227 2613 Size: 2118 390 106 5334 Fragments arranged by size: 5334 2118 390 106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2770 3133 3660 4437 6073 6938 7236 2770 Size: 363 527 777 1636 865 298 3482 Fragments arranged by size: 3482 1636 865 777 527 363 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 6611 882 Size: 617 1041 4071 2219 Fragments arranged by size: 4071 2219 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 5245 5739 493 Size: 711 4041 494 2702 Fragments arranged by size: 4041 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7942 7942 Size: 7948 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 5755 6137 6772 Size: 53 83 186 936 630 3592 382 635 Cuts at: 6772 275 Size: 1451 Fragments arranged by size: 3592 1451 936 635 630 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4028 6381 6779 7053 7200 7611 4028 Size: 2353 398 274 147 411 4365 Fragments arranged by size: 4365 2353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2664 2694 3540 3570 4348 Size: 30 165 30 1261 30 846 30 778 Cuts at: 4348 4378 4405 4435 1178 Size: 30 27 30 4691 Fragments arranged by size: 4691 1261 846 778 165 30 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2527 3846 4287 4927 1186 Size: 1341 1319 441 640 4207 Fragments arranged by size: 4207 1341 1319 640 441 BseYI C'CCAG_C Cuts at: 3831 4126 4425 5076 5145 5178 5208 7151 7647 Size: 295 299 651 69 33 30 1943 496 Cuts at: 7647 3831 Size: 4132 Fragments arranged by size: 4132 1943 651 496 299 295 69 33 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3530 4130 4268 4788 1163 Size: 573 1794 600 138 520 4323 Fragments arranged by size: 4323 1794 600 573 520 138 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 5398 6159 7187 1540 Size: 275 186 54 3343 761 1028 2301 Fragments arranged by size: 3343 2301 1028 761 275 186 54 BsiHKAI G_wGCw'C Cuts at: 2908 3416 4297 4366 4937 6009 6094 7165 2908 Size: 508 881 69 571 1072 85 1071 3691 Fragments arranged by size: 3691 1072 1071 881 571 508 85 69 BsmI GAATG_Cn' Cuts at: 2668 4557 5459 5552 2668 Size: 1889 902 93 5064 Fragments arranged by size: 5064 1889 902 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2526 2712 2908 3276 3416 3496 3886 Size: 399 736 186 196 368 140 80 390 Cuts at: 3886 4297 4366 4779 4937 5192 5228 5267 6009 Size: 411 69 413 158 255 36 39 742 Cuts at: 6009 6094 7165 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 736 413 411 399 390 368 255 196 186 158 140 85 80 69 39 36 BspEI T'CCGG_A Cuts at: 4028 7611 4028 Size: 3583 4365 BspHI T'CATG_A Cuts at: 5837 7567 5837 Size: 1730 6218 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2144 2887 3340 4144 4330 5303 844 Size: 1300 743 453 804 186 973 3489 Fragments arranged by size: 3489 1300 973 804 743 453 186 BsrBI CCG'CTC Cuts at: 1394 1823 5835 1394 Size: 429 4012 3507 Fragments arranged by size: 4012 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2106 3133 4026 6443 6617 65 Size: 1183 858 1027 893 2417 174 1396 Fragments arranged by size: 2417 1396 1183 1027 893 858 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2159 6592 6779 1700 Size: 110 349 4433 187 2869 Fragments arranged by size: 4433 2869 349 187 110 BsrGI T'GTAC_A Cuts at: 96 3991 96 Size: 3895 4053 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7948 BssSI C'ACGA_G Cuts at: 6008 7020 6008 Size: 1012 6936 BstAPI GCAn_nnn'nTGC Cuts at: 794 4136 4184 4868 794 Size: 3342 48 684 3874 Fragments arranged by size: 3874 3342 684 48 BstBI TT'CG_AA Cuts at: 2007 6752 2007 Size: 4745 3203 BstEII G'GTnAC_C Cuts at: 1184 2726 1184 Size: 1542 6406 BstXI CCAn_nnnn'nTGG Cuts at: 4160 4160 Size: 7948 BstYI r'GATC_y Cuts at: 1972 2382 2464 2481 3651 4987 5278 5645 6030 Size: 410 82 17 1170 1336 291 367 385 Cuts at: 6030 6047 7499 7585 7597 7943 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1336 1170 410 385 367 346 291 86 82 17 17 12 BstZ17I GTA'TAC Cuts at: 3626 3626 Size: 7948 BtgI C'CryG_G Cuts at: 513 1065 2049 513 Size: 552 984 6412 Fragments arranged by size: 6412 984 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3744 5202 487 Size: 683 56 2518 1458 3233 Fragments arranged by size: 3233 2518 1458 683 56 BtsI GCAGTG_nn' Cuts at: 3166 4185 4251 5464 6258 6278 3166 Size: 1019 66 1213 794 20 4836 Fragments arranged by size: 4836 1213 1019 794 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7913 DraI TTT'AAA Cuts at: 3280 5252 3280 Size: 1972 5976 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6955 809 Size: 507 5639 1802 Fragments arranged by size: 5639 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2754 4162 Size: 54 1425 50 275 18 222 702 1408 Cuts at: 4162 5112 5395 6284 7913 8 Size: 950 283 889 1629 43 Fragments arranged by size: 1629 1425 1408 950 889 702 283 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2052 5395 1537 Size: 275 240 3343 4090 Fragments arranged by size: 4090 3343 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 2476 2538 5878 1353 Size: 1123 62 3340 3423 Fragments arranged by size: 3423 3340 1123 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2187 2250 2340 6535 6909 7055 Size: 1064 138 598 63 90 4195 374 146 Cuts at: 7055 387 Size: 1280 Fragments arranged by size: 4195 1280 1064 598 374 146 138 90 63 EcoICRI GAG'CTC Cuts at: 4935 4935 Size: 7948 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 3102 3692 3771 3843 3966 4260 Size: 393 566 904 590 79 72 123 294 Cuts at: 4260 5102 5123 6011 6608 7395 7663 1239 Size: 842 21 888 597 787 268 1524 Fragments arranged by size: 1524 904 888 842 787 597 590 566 393 294 268 123 79 72 21 EcoNI CCTnn'n_nnAGG Cuts at: 2288 2288 Size: 7948 EcoO109I rG'GnC_Cy Cuts at: 5035 5035 Size: 7948 EcoRI G'AATT_C Cuts at: 5257 5635 5257 Size: 378 7570 FspI TGC'GCA Cuts at: 6454 6454 Size: 7948 HaeII r_GCGC'y Cuts at: 1678 2079 2136 7095 1678 Size: 401 57 4959 2531 Fragments arranged by size: 4959 2531 401 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3958 3990 4327 4359 4919 Size: 32 135 32 2386 32 337 32 560 Cuts at: 4919 4951 6589 6621 6663 6695 1373 Size: 32 1638 32 42 32 2626 Fragments arranged by size: 2626 2386 1638 560 337 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2971 3229 4413 4643 5292 5538 Size: 988 281 1033 258 1184 230 649 246 Cuts at: 5538 669 Size: 3079 Fragments arranged by size: 3079 1184 1033 988 649 281 258 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 7948 HpaI GTT'AAC Cuts at: 2971 3229 4413 5538 2971 Size: 258 1184 1125 5381 Fragments arranged by size: 5381 1184 1125 258 KpnI G_GTAC'C Cuts at: 2160 5273 2160 Size: 3113 4835 MfeI C'AATT_G Cuts at: 4382 4737 5547 4382 Size: 355 810 6783 Fragments arranged by size: 6783 810 355 MluI A'CGCG_T Cuts at: 7824 7824 Size: 7948 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2743 3598 7062 7246 1305 Size: 1438 855 3464 184 2007 Fragments arranged by size: 3464 2007 1438 855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 4164 5114 10 Size: 54 1425 343 924 1408 950 2844 Fragments arranged by size: 2844 1425 1408 950 924 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5906 6265 6424 518 Size: 648 4740 359 159 2042 Fragments arranged by size: 4740 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2063 2121 2299 3176 4102 4772 4900 Size: 445 12 58 178 877 926 670 128 Cuts at: 4900 5080 5149 5364 6041 7189 7434 1606 Size: 180 69 215 677 1148 245 2120 Fragments arranged by size: 2120 1148 926 877 677 670 445 245 215 180 178 128 69 58 12 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7396 NdeI CA'TA_TG Cuts at: 387 387 Size: 7948 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7948 NotI GC'GGCC_GC Cuts at: 2052 5395 2052 Size: 3343 4605 NspI r_CATG'y Cuts at: 1132 1229 5306 6851 1132 Size: 97 4077 1545 2229 Fragments arranged by size: 4077 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7948 PciI A'CATG_T Cuts at: 6847 6847 Size: 7948 PflMI CCAn_nnn'nTGG Cuts at: 1108 3580 1108 Size: 2472 5476 PfoI T'CCnGG_A Cuts at: 2562 2562 Size: 7948 PmeI GTTT'AAAC Cuts at: 5252 5252 Size: 7948 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6017 6049 7530 7562 6017 Size: 32 1481 32 6403 Fragments arranged by size: 6403 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5035 5035 Size: 7948 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7948 PsiI TTA'TAA Cuts at: 5518 5518 Size: 7948 PstI C_TGCA'G Cuts at: 830 2068 2233 2304 3181 4048 4141 4246 4483 Size: 1238 165 71 877 867 93 105 237 Cuts at: 4483 5154 5300 830 Size: 671 146 3478 Fragments arranged by size: 3478 1238 877 867 671 237 165 146 105 93 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7948 PvuII CAG'CTG Cuts at: 2299 3176 4102 5080 5149 2299 Size: 877 926 978 69 5098 Fragments arranged by size: 5098 978 926 877 69 SacI G_AGCT'C Cuts at: 4937 4937 Size: 7948 SacII CC_GC'GG Cuts at: 2052 2052 Size: 7948 SalI G'TCGA_C Cuts at: 1936 5290 1936 Size: 3354 4594 SbfI CC_TGCA'GG Cuts at: 4141 5300 4141 Size: 1159 6789 ScaI AGT'ACT Cuts at: 2914 6196 7908 2914 Size: 3282 1712 2954 Fragments arranged by size: 3282 2954 1712 SfcI C'TryA_G Cuts at: 826 1045 2064 2229 2300 2916 3177 3336 4044 Size: 219 1019 165 71 616 261 159 708 Cuts at: 4044 4137 4242 4479 5150 5296 6431 7112 7303 Size: 93 105 237 671 146 1135 681 191 Cuts at: 7303 826 Size: 1471 Fragments arranged by size: 1471 1135 1019 708 681 671 616 261 237 219 191 165 159 146 105 93 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7948 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7948 SmaI CCC'GGG Cuts at: 1872 5275 1872 Size: 3403 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2785 3112 3675 4452 6052 6953 Size: 197 926 842 327 563 777 1600 901 Cuts at: 6953 7215 820 Size: 262 1553 Fragments arranged by size: 1600 1553 926 901 842 777 563 327 262 197 SnaBI TAC'GTA Cuts at: 493 5245 5739 493 Size: 4752 494 2702 Fragments arranged by size: 4752 2702 494 SpeI A'CTAG_T Cuts at: 152 2951 3416 4358 152 Size: 2799 465 942 3742 Fragments arranged by size: 3742 2799 942 465 SphI G_CATG'C Cuts at: 1132 5306 1132 Size: 4174 3774 SspI AAT'ATT Cuts at: 5 52 3049 4216 5872 5 Size: 47 2997 1167 1656 2081 Fragments arranged by size: 2997 2081 1656 1167 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7948 StyI C'CwwG_G Cuts at: 513 1065 3019 3559 5387 513 Size: 552 1954 540 1828 3074 Fragments arranged by size: 3074 1954 1828 552 540 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5192 5975 5992 6145 6330 6775 325 Size: 1255 3612 783 17 153 185 445 1498 Fragments arranged by size: 3612 1498 1255 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2912 2954 3038 Size: 274 80 33 51 1443 935 42 84 Cuts at: 3038 3661 3991 6194 7906 96 Size: 623 330 2203 1712 138 Fragments arranged by size: 2203 1712 1443 935 623 330 274 138 84 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2664 2696 4053 4080 4085 4112 7908 7940 2664 Size: 32 1357 27 5 27 3796 32 2672 Fragments arranged by size: 3796 2672 1357 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7948 XbaI T'CTAG_A Cuts at: 2798 5284 2798 Size: 2486 5462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2720 3817 5153 2720 Size: 1097 1336 5515 Fragments arranged by size: 5515 1336 1097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7948 XmaI C'CCGG_G Cuts at: 1870 5273 1870 Size: 3403 4545 XmnI GAAnn'nnTTC Cuts at: 4462 4555 6077 4462 Size: 93 1522 6333 Fragments arranged by size: 6333 1522 93 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5756 276 Size: 53 83 186 936 4222 2468 Fragments arranged by size: 4222 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI