Restriction map for
pFN21ASDA1994
(Linear) (Six-base) MAPSORT of: KIAA1994.seq Check: 7127 from: 1 to: 3219
KIAA1994.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 864 2307 3219
Size: 864 1443 912
Fragments arranged by size:
1443 912 864
AccI GT'mk_AC
Cuts at: 0 1602 3219
Size: 1602 1617
Acc65I G'GTAC_C
Cuts at: 0 133 3219
Size: 133 3086
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1669 1748 1943 2237 3100 3219
Size: 175 1494 79 195 294 863 119
Fragments arranged by size:
1494 863 294 195 175 119 79
AfeI AGC'GCT
Cuts at: 0 54 3219
Size: 54 3165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2471 3219
Size: 2471 748
AlwNI CAG_nnn'CTG
Cuts at: 0 2161 2845 3219
Size: 2161 684 374
Fragments arranged by size:
2161 684 374
ApoI r'AATT_y
Cuts at: 0 912 2646 3048 3219
Size: 912 1734 402 171
Fragments arranged by size:
1734 912 402 171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 125 158 3219
Size: 125 33 3061
Fragments arranged by size:
3061 125 33
BanI G'GyrC_C
Cuts at: 0 133 686 1860 3166 3219
Size: 133 553 1174 1306 53
Fragments arranged by size:
1306 1174 553 133 53
BanII G_rGCy'C
Cuts at: 0 503 1253 1473 2756 2914 3205 3219
Size: 503 750 220 1283 158 291 14
Fragments arranged by size:
1283 750 503 291 220 158 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 1675 3088 3219
Size: 1675 1413 131
Fragments arranged by size:
1675 1413 131
BbvCI CC'TCA_GC
Cuts at: 0 590 3219
Size: 590 2629
BciVI GTATCCnnnnn_n'
Cuts at: 0 1738 2083 3219
Size: 1738 345 1136
Fragments arranged by size:
1738 1136 345
BclI T'GATC_A
Cuts at: 0 629 3219
Size: 629 2590
BglII A'GATC_T
Cuts at: 0 359 441 1628 3219
Size: 359 82 1187 1591
Fragments arranged by size:
1591 1187 359 82
BlpI GC'TnA_GC
Cuts at: 0 272 2060 3219
Size: 272 1788 1159
Fragments arranged by size:
1788 1159 272
Bme1580I G_kGCm'C
Cuts at: 0 689 1863 3169 3219
Size: 689 1174 1306 50
Fragments arranged by size:
1306 1174 689 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 763 1175 2009 2318 2679 3105 3219
Size: 763 412 834 309 361 426 114
Fragments arranged by size:
834 763 426 412 361 309 114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1634 2931 3219
Size: 1634 1297 288
Fragments arranged by size:
1634 1297 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1820 3079 3219
Size: 1079 741 1259 140
Fragments arranged by size:
1259 1079 741 140
Bpu10I CC'TnA_GC
Cuts at: 0 590 2708 3098 3204 3219
Size: 590 2118 390 106 15
Fragments arranged by size:
2118 590 390 106 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 1110 1637 2414 3219
Size: 747 363 527 777 805
Fragments arranged by size:
805 777 747 527 363
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 3219
Size: 517 2702
BsaHI Gr'CG_yC
Cuts at: 0 140 3219
Size: 140 3079
BsaWI w'CCGG_w
Cuts at: 0 2005 3219
Size: 2005 1214
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 641 671 1517 1547 2325 2355 2382 2412
Size: 641 30 846 30 778 30 27 30
Cuts at: 2412 3219
Size: 807
Fragments arranged by size:
846 807 778 641 30 30 30 30
27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 504 1823 2264 2904 3219
Size: 504 1319 441 640 315
Fragments arranged by size:
1319 640 504 441 315
BseYI C'CCAG_C
Cuts at: 0 1808 2103 2402 3053 3122 3155 3185 3219
Size: 1808 295 299 651 69 33 30 34
Fragments arranged by size:
1808 651 299 295 69 34 33 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1507 2107 2245 2765 3219
Size: 1507 600 138 520 454
Fragments arranged by size:
1507 600 520 454 138
BsiEI CG_ry'CG
Cuts at: 0 32 3219
Size: 32 3187
BsiHKAI G_wGCw'C
Cuts at: 0 885 1393 2274 2343 2914 3219
Size: 885 508 881 69 571 305
Fragments arranged by size:
885 881 571 508 305 69
BsmI GAATG_Cn'
Cuts at: 0 645 2534 3219
Size: 645 1889 685
Fragments arranged by size:
1889 685 645
Bsp1286I G_dGCh'C
Cuts at: 0 503 689 885 1253 1393 1473 1863 2274
Size: 503 186 196 368 140 80 390 411
Cuts at: 2274 2343 2756 2914 3169 3205 3219
Size: 69 413 158 255 36 14
Fragments arranged by size:
503 413 411 390 368 255 196 186
158 140 80 69 36 14
BspEI T'CCGG_A
Cuts at: 0 2005 3219
Size: 2005 1214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 864 1317 2121 2307 3219
Size: 121 743 453 804 186 912
Fragments arranged by size:
912 804 743 453 186 121
BsrDI GCAATG_nn'
Cuts at: 0 83 1110 2003 3219
Size: 83 1027 893 1216
Fragments arranged by size:
1216 1027 893 83
BsrFI r'CCGG_y
Cuts at: 0 136 3219
Size: 136 3083
BsrGI T'GTAC_A
Cuts at: 0 1968 3219
Size: 1968 1251
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2113 2161 2845 3219
Size: 2113 48 684 374
Fragments arranged by size:
2113 684 374 48
BstEII G'GTnAC_C
Cuts at: 0 703 3219
Size: 703 2516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2137 3219
Size: 2137 1082
BstYI r'GATC_y
Cuts at: 0 359 441 458 1628 2964 3219
Size: 359 82 17 1170 1336 255
Fragments arranged by size:
1336 1170 359 255 82 17
BstZ17I GTA'TAC
Cuts at: 0 1603 3219
Size: 1603 1616
BtgI C'CryG_G
Cuts at: 0 26 3219
Size: 26 3193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1721 3179 3219
Size: 1721 1458 40
Fragments arranged by size:
1721 1458 40
BtsI GCAGTG_nn'
Cuts at: 0 1143 2162 2228 3219
Size: 1143 1019 66 991
Fragments arranged by size:
1143 1019 991 66
DraI TTT'AAA
Cuts at: 0 1257 3219
Size: 1257 1962
EaeI y'GGCC_r
Cuts at: 0 29 731 2139 3089 3219
Size: 29 702 1408 950 130
Fragments arranged by size:
1408 950 702 130 29
EagI C'GGCC_G
Cuts at: 0 29 3219
Size: 29 3190
EarI CTCTTCn'nnn_
Cuts at: 0 453 515 3219
Size: 453 62 2704
Fragments arranged by size:
2704 453 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 164 227 317 3219
Size: 164 63 90 2902
Fragments arranged by size:
2902 164 90 63
EcoICRI GAG'CTC
Cuts at: 0 2912 3219
Size: 2912 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1079 1669 1748 1820 1943 2237 3079
Size: 175 904 590 79 72 123 294 842
Cuts at: 3079 3100 3219
Size: 21 119
Fragments arranged by size:
904 842 590 294 175 123 119 79
72 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 265 3219
Size: 265 2954
EcoO109I rG'GnC_Cy
Cuts at: 0 3012 3219
Size: 3012 207
HaeII r_GCGC'y
Cuts at: 0 56 113 3219
Size: 56 57 3106
Fragments arranged by size:
3106 57 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1935 1967 2304 2336 2896 2928 3219
Size: 1935 32 337 32 560 32 291
Fragments arranged by size:
1935 560 337 291 32 32 32
HincII GTy'rAC
Cuts at: 0 948 1206 2390 2620 3219
Size: 948 258 1184 230 599
Fragments arranged by size:
1184 948 599 258 230
HpaI GTT'AAC
Cuts at: 0 948 1206 2390 3219
Size: 948 258 1184 829
Fragments arranged by size:
1184 948 829 258
KpnI G_GTAC'C
Cuts at: 0 137 3219
Size: 137 3082
MfeI C'AATT_G
Cuts at: 0 2359 2714 3219
Size: 2359 355 505
Fragments arranged by size:
2359 505 355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 1575 3219
Size: 720 855 1644
Fragments arranged by size:
1644 855 720
MscI TGG'CCA
Cuts at: 0 733 2141 3091 3219
Size: 733 1408 950 128
Fragments arranged by size:
1408 950 733 128
MspA1I CmG'CkG
Cuts at: 0 28 40 98 276 1153 2079 2749 2877
Size: 28 12 58 178 877 926 670 128
Cuts at: 2877 3057 3126 3219
Size: 180 69 93
Fragments arranged by size:
926 877 670 180 178 128 93 69
58 28 12
NotI GC'GGCC_GC
Cuts at: 0 29 3219
Size: 29 3190
PflMI CCAn_nnn'nTGG
Cuts at: 0 1557 3219
Size: 1557 1662
PfoI T'CCnGG_A
Cuts at: 0 539 3219
Size: 539 2680
PpuMI rG'GwC_Cy
Cuts at: 0 3012 3219
Size: 3012 207
PstI C_TGCA'G
Cuts at: 0 45 210 281 1158 2025 2118 2223 2460
Size: 45 165 71 877 867 93 105 237
Cuts at: 2460 3131 3219
Size: 671 88
Fragments arranged by size:
877 867 671 237 165 105 93 88
71 45
PvuII CAG'CTG
Cuts at: 0 276 1153 2079 3057 3126 3219
Size: 276 877 926 978 69 93
Fragments arranged by size:
978 926 877 276 93 69
SacI G_AGCT'C
Cuts at: 0 2914 3219
Size: 2914 305
SacII CC_GC'GG
Cuts at: 0 29 3219
Size: 29 3190
SbfI CC_TGCA'GG
Cuts at: 0 2118 3219
Size: 2118 1101
ScaI AGT'ACT
Cuts at: 0 891 3219
Size: 891 2328
SfcI C'TryA_G
Cuts at: 0 41 206 277 893 1154 1313 2021 2114
Size: 41 165 71 616 261 159 708 93
Cuts at: 2114 2219 2456 3127 3219
Size: 105 237 671 92
Fragments arranged by size:
708 671 616 261 237 165 159 105
93 92 71 41
SmlI C'TyrA_G
Cuts at: 0 762 1089 1652 2429 3219
Size: 762 327 563 777 790
Fragments arranged by size:
790 777 762 563 327
SpeI A'CTAG_T
Cuts at: 0 928 1393 2335 3219
Size: 928 465 942 884
Fragments arranged by size:
942 928 884 465
SspI AAT'ATT
Cuts at: 0 1026 2193 3219
Size: 1026 1167 1026
Fragments arranged by size:
1167 1026 1026
StyI C'CwwG_G
Cuts at: 0 996 1536 3219
Size: 996 540 1683
Fragments arranged by size:
1683 996 540
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3169 3219
Size: 3169 50
TatI w'GTAC_w
Cuts at: 0 889 931 1015 1638 1968 3219
Size: 889 42 84 623 330 1251
Fragments arranged by size:
1251 889 623 330 84 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 673 2030 2057 2062 2089 3219
Size: 641 32 1357 27 5 27 1130
Fragments arranged by size:
1357 1130 641 32 27 27 5
XbaI T'CTAG_A
Cuts at: 0 775 3219
Size: 775 2444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 697 1794 3130 3219
Size: 697 1097 1336 89
Fragments arranged by size:
1336 1097 697 89
XmnI GAAnn'nnTTC
Cuts at: 0 2439 2532 3219
Size: 2439 93 687
Fragments arranged by size:
2439 687 93
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AlfI AlwNI ApoI
BaeI BanI BanII BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HpaI KpnI MfeI MmeI MscI
MspA1I NotI PflMI PfoI PpuMI PstI PvuII SacI
SacII SbfI ScaI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BcgI BfrBI BglI BmgBI BmtI BsaAI BsaBI
BsiWI BsmBI BspHI BsrBI BssHII BssSI BstBI Bsu36I
ClaI CspCI DraIII DrdI EcoRI EcoRV FalI FseI
FspI FspAI HindIII KasI MluI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SapI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI Tth111I XhoI XmaI ZraI