(Circular) (Six-base) MAPSORT of: pFN21ASDA1993.seq Check: 6264 from: 1 to: 6241 pFN21ASDA1993 6241 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3030 3030 Size: 6241 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 2802 3129 3356 3562 2802 Size: 327 227 206 5481 Fragments arranged by size: 5481 327 227 206 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2345 2478 3420 4304 5688 5956 1632 Size: 710 3 133 942 884 1384 268 1917 Fragments arranged by size: 1917 1384 942 884 710 268 133 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 5140 6117 1203 Size: 3937 977 1327 Fragments arranged by size: 3937 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 2732 1166 Size: 1566 4675 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2618 1184 Size: 1434 4807 AlwNI CAG_nnn'CTG Cuts at: 3043 3521 3984 5556 3043 Size: 478 463 1572 3728 Fragments arranged by size: 3728 1572 478 463 ApaLI G'TGCA_C Cuts at: 2386 4298 5454 2386 Size: 1912 1156 3173 Fragments arranged by size: 3173 1912 1156 ApoI r'AATT_y Cuts at: 1493 3507 3550 3776 3928 6037 1493 Size: 2014 43 226 152 2109 1697 Fragments arranged by size: 2109 2014 1697 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2468 3083 3102 3425 3566 1870 Size: 73 126 399 615 19 323 141 4545 Fragments arranged by size: 4545 615 399 323 141 126 73 19 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2901 2934 3266 3299 2901 Size: 33 332 33 5843 Fragments arranged by size: 5843 332 33 33 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2802 3129 3356 3562 5017 Size: 325 217 627 1015 327 227 206 1455 Cuts at: 5017 618 Size: 1842 Fragments arranged by size: 1842 1455 1015 627 327 325 227 217 206 BanII G_rGCy'C Cuts at: 1391 2170 2855 3056 3560 1391 Size: 779 685 201 504 4072 Fragments arranged by size: 4072 779 685 504 201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4432 4466 1903 Size: 34 2495 34 3678 Fragments arranged by size: 3678 2495 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 1548 Size: 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4852 136 Size: 107 122 71 631 605 3180 1525 Fragments arranged by size: 3180 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2520 3327 6236 2520 Size: 807 2909 2525 Fragments arranged by size: 2909 2525 807 BlpI GC'TnA_GC Cuts at: 2969 3658 2969 Size: 689 5552 Bme1580I G_kGCm'C Cuts at: 1790 2070 2390 2631 2995 4302 5458 1790 Size: 280 320 241 364 1307 1156 2573 Fragments arranged by size: 2573 1307 1156 364 320 280 241 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2507 3972 4930 Size: 416 53 83 399 24 1090 1465 958 Cuts at: 4930 442 Size: 1753 Fragments arranged by size: 1753 1465 1090 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2647 2647 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2855 3299 4901 1239 Size: 1616 444 1602 2579 Fragments arranged by size: 2579 1616 1602 444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4366 5231 5529 4366 Size: 865 298 5078 Fragments arranged by size: 5078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2679 2694 4904 882 Size: 617 1180 15 2210 2219 Fragments arranged by size: 2219 2210 1180 617 15 BsaAI yAC'GTr Cuts at: 493 1204 3445 3538 4032 493 Size: 711 2241 93 494 2702 Fragments arranged by size: 2702 2241 711 494 93 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4048 4430 5065 275 Size: 53 83 186 936 2515 382 635 1451 Fragments arranged by size: 2515 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2574 4674 5072 5346 5493 5904 2574 Size: 2100 398 274 147 411 2911 Fragments arranged by size: 2911 2100 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2768 2798 2867 2897 1178 Size: 30 165 30 1365 30 69 30 4522 Fragments arranged by size: 4522 1365 165 69 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2517 2636 2773 2997 3124 1186 Size: 1331 119 137 224 127 4303 Fragments arranged by size: 4303 1331 224 137 127 119 BseYI C'CCAG_C Cuts at: 2591 2765 5444 5940 2591 Size: 174 2679 496 2892 Fragments arranged by size: 2892 2679 496 174 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3008 3068 1163 Size: 573 1272 60 4336 Fragments arranged by size: 4336 1272 573 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2761 3691 4452 5480 1540 Size: 275 186 760 930 761 1028 2301 Fragments arranged by size: 2301 1028 930 761 760 275 186 BsiHKAI G_wGCw'C Cuts at: 2390 2871 4302 4387 5458 2390 Size: 481 1431 85 1071 3173 Fragments arranged by size: 3173 1431 1071 481 85 BsmI GAATG_Cn' Cuts at: 3752 3845 3752 Size: 93 6148 BsmBI CGTCTCn'nnnn_ Cuts at: 2576 2576 Size: 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2170 2390 2631 2855 2871 2995 Size: 399 280 100 220 241 224 16 124 Cuts at: 2995 3056 3560 4302 4387 5458 1391 Size: 61 504 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 399 280 241 224 220 124 100 85 61 16 BspEI T'CCGG_A Cuts at: 2574 5904 2574 Size: 3330 2911 BspHI T'CATG_A Cuts at: 4130 5860 4130 Size: 1730 4511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3030 3042 3596 844 Size: 2186 12 554 3489 Fragments arranged by size: 3489 2186 554 12 BsrBI CCG'CTC Cuts at: 1394 1823 4128 1394 Size: 429 2305 3507 Fragments arranged by size: 3507 2305 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4736 4910 65 Size: 1183 3488 174 1396 Fragments arranged by size: 3488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 4885 5072 1700 Size: 110 950 2125 187 2869 Fragments arranged by size: 2869 2125 950 187 110 BsrGI T'GTAC_A Cuts at: 96 2140 96 Size: 2044 4197 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 4301 5313 4301 Size: 1012 5229 BstAPI GCAn_nnn'nTGC Cuts at: 794 2183 794 Size: 1389 4852 BstBI TT'CG_AA Cuts at: 2007 5045 2007 Size: 3038 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 6241 BstYI r'GATC_y Cuts at: 1972 2394 2520 3327 3571 3938 4323 4340 5792 Size: 422 126 807 244 367 385 17 1452 Cuts at: 5792 5878 5890 6236 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 807 422 385 367 346 244 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2638 513 Size: 552 1573 4116 Fragments arranged by size: 4116 1573 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5502 Fragments arranged by size: 5502 683 56 BtsI GCAGTG_nn' Cuts at: 2375 2572 2653 2847 3757 4551 4571 2375 Size: 197 81 194 910 794 20 4045 Fragments arranged by size: 4045 910 794 197 194 81 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3229 3264 529 Size: 35 2665 35 3506 Fragments arranged by size: 3506 2665 35 35 DraI TTT'AAA Cuts at: 3545 3545 Size: 6241 DraIII CAC_nnn'GTG Cuts at: 3517 3517 Size: 6241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3688 4577 6206 Size: 54 1425 50 275 18 1858 889 1629 Cuts at: 6206 8 Size: 43 Fragments arranged by size: 1858 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 4171 1353 Size: 1101 1717 3423 Fragments arranged by size: 3423 1717 1101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 4828 5202 5348 387 Size: 1064 138 510 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 510 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2345 2478 2855 3299 3420 4304 Size: 393 710 3 133 377 444 121 884 Cuts at: 4304 4901 5688 5956 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 884 787 710 597 444 393 377 268 133 121 3 EcoNI CCTnn'n_nnAGG Cuts at: 3047 3047 Size: 6241 EcoO109I rG'GnC_Cy Cuts at: 3040 3040 Size: 6241 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2198 3209 2198 Size: 1011 5230 FspI TGC'GCA Cuts at: 2606 4747 2606 Size: 2141 4100 HaeII r_GCGC'y Cuts at: 1678 5388 1678 Size: 3710 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2245 2277 2423 2455 2512 Size: 32 135 32 673 32 146 32 57 Cuts at: 2512 2544 2768 2800 4882 4914 4956 4988 1373 Size: 32 224 32 2082 32 42 32 2626 Fragments arranged by size: 2626 2082 673 224 146 135 57 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2437 3585 3831 669 Size: 988 281 499 1148 246 3079 Fragments arranged by size: 3079 1148 988 499 281 246 HindIII A'AGCT_T Cuts at: 748 2598 2925 748 Size: 1850 327 4064 Fragments arranged by size: 4064 1850 327 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KpnI G_GTAC'C Cuts at: 2806 3133 3360 3566 2806 Size: 327 227 206 5481 Fragments arranged by size: 5481 327 227 206 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2407 2781 2799 5355 5539 1305 Size: 1102 374 18 2556 184 2007 Fragments arranged by size: 2556 2007 1102 374 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4419 Fragments arranged by size: 4419 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 2813 3303 4199 4558 4717 518 Size: 648 1566 81 490 896 359 159 2042 Fragments arranged by size: 2042 1566 896 648 490 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2320 2640 2769 3321 3657 4334 5482 5727 Size: 714 320 129 552 336 677 1148 245 Cuts at: 5727 1606 Size: 2120 Fragments arranged by size: 2120 1148 714 677 552 336 320 245 129 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5689 NdeI CA'TA_TG Cuts at: 387 3216 387 Size: 2829 3412 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 3208 3599 5144 1132 Size: 97 1979 391 1545 2229 Fragments arranged by size: 2229 1979 1545 391 97 PasI CC'CwG_GG Cuts at: 1348 2788 1348 Size: 1440 4801 PciI A'CATG_T Cuts at: 5140 5140 Size: 6241 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6241 PfoI T'CCnGG_A Cuts at: 2205 3497 2205 Size: 1292 4949 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PstI C_TGCA'G Cuts at: 830 2118 2350 3039 3593 830 Size: 1288 232 689 554 3478 Fragments arranged by size: 3478 1288 689 554 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2320 2769 2320 Size: 449 5792 SacII CC_GC'GG Cuts at: 2641 2641 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SbfI CC_TGCA'GG Cuts at: 2118 3039 3593 2118 Size: 921 554 4766 Fragments arranged by size: 4766 921 554 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SfcI C'TryA_G Cuts at: 826 1045 2114 2346 3035 3589 4724 5405 5596 Size: 219 1069 232 689 554 1135 681 191 Cuts at: 5596 826 Size: 1471 Fragments arranged by size: 1471 1135 1069 689 681 554 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 3085 3104 3568 1872 Size: 1213 19 464 4545 Fragments arranged by size: 4545 1213 464 19 SmlI C'TyrA_G Cuts at: 820 1017 1943 4345 5246 5508 820 Size: 197 926 2402 901 262 1553 Fragments arranged by size: 2402 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3538 4032 493 Size: 3045 494 2702 Fragments arranged by size: 3045 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6241 StyI C'CwwG_G Cuts at: 513 1065 3352 3680 513 Size: 552 2287 328 3074 Fragments arranged by size: 3074 2287 552 328 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2780 3402 4268 4285 4438 4623 5068 Size: 1255 1200 622 866 17 153 185 445 Cuts at: 5068 325 Size: 1498 Fragments arranged by size: 1498 1255 1200 866 622 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 2140 3258 Size: 274 80 33 51 1443 96 67 1118 Cuts at: 3258 4487 6199 96 Size: 1229 1712 138 Fragments arranged by size: 1712 1443 1229 1118 274 138 96 80 67 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2113 2145 2867 2899 6201 6233 2113 Size: 32 722 32 3302 32 2121 Fragments arranged by size: 3302 2121 722 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6241 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 3083 3102 3566 1870 Size: 1213 19 464 4545 Fragments arranged by size: 4545 1213 464 19 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI