Restriction map for
pFN21ASDA1991
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HpaI KasI KpnI MluI
MluNI MroI MscI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
PvuII RcaI RsrII SacI SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 719 AACGTT AA^CGTT
Aor51HI 416 AGCGCT AGC^GCT
AspHI 515 GAGCAC G[AT]GC[AT]^C
AspI 1957 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1286 GGATCC G^GATCC
BanI 209 GGTGCC G^G[CT][GA]CC
BclI 1503 TGATCA T^GATCA
BglII 1307 AGATCT A^GATCT
BseRI 1172 CTCCTC GAGGAG(10/8)
BsgI 728 CTGCAC GTGCAG(16/14)
BsiEI 184 CGGCCG CG[GA][CT]^CG
BsiHKAI 515 GAGCAC G[AT]GC[AT]^C
BsrDI 1112 GCAATG GCAATG(2/0)
Bst1107I 1476 GTATAC GTA^TAC
BstXI 2102 CCAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1476 GTATAC GTA^TAC
EagI 184 CGGCCG C^GGCCG
EclXI 184 CGGCCG C^GGCCG
Eco47III 416 AGCGCT AGC^GCT
Eco52I 184 CGGCCG C^GGCCG
EcoNI 760 CCTTCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1503 TGATCA T^GATCA
HaeII 416 AGCGCT [GA]GCGC^[CT]
MfeI 1774 CAATTG C^AATTG
MslI 945 CACAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1774 CAATTG C^AATTG
NspI 2033 GCATGT [GA]CATG^[CT]
PaeR7I 764 CTCGAG C^TCGAG
PflFI 1957 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1463 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 719 AACGTT AA^CGTT
SfiI 67 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 300 CCCGGG CCC^GGG
Tth111I 1957 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1928 CCAGTGGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 764 CTCGAG C^TCGAG
XmaCI 300 CCCGGG C^CCGGG
XmaI 300 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1043 GTCTAC GT^[AC][GT]AC
1476 GTATAC
BbsI 1779 GAAGAC GAAGAC(2/6)
2002 GAAGAC
BciVI 642 GGATAC GTATCC(6/5)
1403 GTATCC
BpuAI 1779 GAAGAC GAAGAC(2/6)
2002 GAAGAC
BsaBI 1498 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2083 GATCAGTATC
BsmFI 434 GGGAC GGGAC(10/14)
1618 GGGAC
BsrBI 50 CCGCTC CCGCTC(-3/-3)
1318 GAGCGG
BsrFI 68 GCCGGC [GA]^CCGG[CT]
2063 GCCGGC
BstF5I 1064 CATCC GGATG(2/0)
669 GGATG
Cfr10I 68 GCCGGC [GA]^CCGG[CT]
2063 GCCGGC
FokI 1064 CATCC GGATG(9/13)
669 GGATG
HincII 990 GTCAAC GT[CT]^[GA]AC
1656 GTCAAC
HindII 990 GTCAAC GT[CT]^[GA]AC
1656 GTCAAC
HindIII 564 AAGCTT A^AGCTT
1984 AAGCTT
HphI 1162 TCACC GGTGA(8/7)
1709 TCACC
MaeII 720 ACGT A^CGT
732 ACGT
MamI 1498 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2083 GATCAGTATC
NaeI 68 GCCGGC GCC^GGC
2063 GCCGGC
NgoMIV 68 GCCGGC G^CCGGC
2063 GCCGGC
SapI 903 GAAGAGC GCTCTTC(1/4)
1834 GAAGAGC
ScaI 19 AGTACT AGT^ACT
699 AGTACT
SfaNI 1666 GATGC GCATC(5/9)
1121 GCATC
SmlI 567 CTTGAG C^T[CT][GA]AG
764 CTCGAG
TfiI 193 GAATC G^A[AT]TC
742 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AvaI AvaII BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmBI
BsoBI Bsp1286I BspMI BsrI BssKI
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I KspI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII