Restriction map for
pFN21ASDA1991
(Circular) (Six-base) MAPSORT of: pFN21ASDA1991.seq Check: 5624 from: 1 to: 6853
pFN21ASDA1991 6853 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4663 278
Size: 53 83 186 936 3127 2468
Fragments arranged by size:
3127 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3067 3500 4196 1937
Size: 1130 433 696 4594
Fragments arranged by size:
4594 1130 696 433
Acc65I G'GTAC_C
Cuts at: 4174 4174
Size: 6853
AclI AA'CG_TT
Cuts at: 1563 2743 4980 5353 1563
Size: 1180 2237 373 3063
Fragments arranged by size:
3063 2237 1180 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2655 3058 3441 3995 4916 6300 6568 1632
Size: 1023 403 383 554 921 1384 268 1917
Fragments arranged by size:
1917 1384 1023 921 554 403 383 268
AfeI AGC'GCT
Cuts at: 2441 2441
Size: 6853
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6656
AflIII A'CryG_T
Cuts at: 1203 5752 6729 1203
Size: 4549 977 1327
Fragments arranged by size:
4549 1327 977
AgeI A'CCGG_T
Cuts at: 5684 5684
Size: 6853
AhdI GACnn_n'nnGTC
Cuts at: 5582 5582
Size: 6853
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6853
AlwNI CAG_nnn'CTG
Cuts at: 4596 6168 4596
Size: 1572 5281
ApaI G_GGCC'C
Cuts at: 2137 2324 2365 2377 2484 2137
Size: 187 41 12 107 6506
Fragments arranged by size:
6506 187 107 41 12
ApaLI G'TGCA_C
Cuts at: 4910 6066 4910
Size: 1156 5697
ApoI r'AATT_y
Cuts at: 1493 4162 4388 4540 6649 1493
Size: 2669 226 152 2109 1697
Fragments arranged by size:
2669 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5407 160
Size: 872 4375 1606
Fragments arranged by size:
4375 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2323 2435 2787 4178 1870
Size: 73 103 277 112 352 1391 4545
Fragments arranged by size:
4545 1391 352 277 112 103 73
BamHI G'GATC_C
Cuts at: 3309 4183 4550 3309
Size: 874 367 5612
Fragments arranged by size:
5612 874 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 4174 5629 618
Size: 325 217 627 445 1942 1455 1842
Fragments arranged by size:
1942 1842 1455 627 445 325 217
BanII G_rGCy'C
Cuts at: 1391 2137 2244 2324 2365 2377 2484 4172 1391
Size: 746 107 80 41 12 107 1688 4072
Fragments arranged by size:
4072 1688 746 107 107 80 41 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3809 4032 928
Size: 971 1910 223 3749
Fragments arranged by size:
3749 1910 971 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5044 5078 1903
Size: 34 3107 34 3678
Fragments arranged by size:
3678 3107 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2659 3437 4745 5955 2659
Size: 778 1308 1210 3557
Fragments arranged by size:
3557 1308 1210 778
BclI T'GATC_A
Cuts at: 1548 3526 1548
Size: 1978 4875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2097 2206 2315
Size: 107 122 71 631 605 425 109 109
Cuts at: 2315 5464 136
Size: 3149 1525
Fragments arranged by size:
3149 1525 631 605 425 122 109 109
107 71
BglII A'GATC_T
Cuts at: 3330 6848 3330
Size: 3518 3335
BlpI GC'TnA_GC
Cuts at: 4270 4270
Size: 6853
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2324 2365 2377 2484 4914 6070 1790
Size: 347 187 41 12 107 2430 1156 2573
Fragments arranged by size:
2573 2430 1156 347 187 107 41 12
BmgBI CAC'GTC
Cuts at: 1318 2756 1318
Size: 1438 5415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2139 3977 4584
Size: 416 53 83 399 24 722 1838 607
Cuts at: 4584 5542 442
Size: 958 1753
Fragments arranged by size:
1838 1753 958 722 607 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2964 3669 5513 1239
Size: 1019 706 705 1844 2579
Fragments arranged by size:
2579 1844 1019 706 705
Bpu10I CC'TnA_GC
Cuts at: 2848 2848
Size: 6853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2611 4978 5843 6141 2611
Size: 2367 865 298 3323
Fragments arranged by size:
3323 2367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5516 882
Size: 617 4017 2219
Fragments arranged by size:
4017 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4150 4644 493
Size: 711 2946 494 2702
Fragments arranged by size:
2946 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3525 4110 6847 3525
Size: 585 2737 3531
Fragments arranged by size:
3531 2737 585
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4660 5042 5677 275
Size: 53 83 186 936 3127 382 635 1451
Fragments arranged by size:
3127 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5286 5684 5958 6105 6516 5286
Size: 398 274 147 411 5623
Fragments arranged by size:
5623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2828 2858 2962 2992 3131
Size: 30 165 30 1425 30 104 30 139
Cuts at: 3131 3161 1178
Size: 30 4870
Fragments arranged by size:
4870 1425 165 139 104 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3186 1186
Size: 2000 4853
BseYI C'CCAG_C
Cuts at: 2358 2407 6056 6552 2358
Size: 49 3649 496 2659
Fragments arranged by size:
3649 2659 496 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2736 1163
Size: 573 1000 5280
Fragments arranged by size:
5280 1000 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2210 4303 5064 6092 1540
Size: 275 186 209 2093 761 1028 2301
Fragments arranged by size:
2301 2093 1028 761 275 209 186
BsiHKAI G_wGCw'C
Cuts at: 2542 4914 4999 6070 2542
Size: 2372 85 1071 3325
Fragments arranged by size:
3325 2372 1071 85
BsmI GAATG_Cn'
Cuts at: 4364 4457 4364
Size: 93 6760
BsmBI CGTCTCn'nnnn_
Cuts at: 2105 2762 3040 2105
Size: 657 278 5918
Fragments arranged by size:
5918 657 278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2244 2324 2365 2377 2484 2542
Size: 399 347 107 80 41 12 107 58
Cuts at: 2542 4172 4914 4999 6070 1391
Size: 1630 742 85 1071 2174
Fragments arranged by size:
2174 1630 1071 742 399 347 107 107
85 80 58 41 12
BspEI T'CCGG_A
Cuts at: 6516 6516
Size: 6853
BspHI T'CATG_A
Cuts at: 4742 6472 4742
Size: 1730 5123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2024 3475 3614 4208 844
Size: 1180 1451 139 594 3489
Fragments arranged by size:
3489 1451 1180 594 139
BsrBI CCG'CTC
Cuts at: 1394 1823 2075 3343 4740 1394
Size: 429 252 1268 1397 3507
Fragments arranged by size:
3507 1397 1268 429 252
BsrDI GCAATG_nn'
Cuts at: 65 1248 3142 5348 5522 65
Size: 1183 1894 2206 174 1396
Fragments arranged by size:
2206 1894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 4086 5497 5684 1700
Size: 110 281 1995 1411 187 2869
Fragments arranged by size:
2869 1995 1411 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6853
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6853
BssSI C'ACGA_G
Cuts at: 4913 5925 4913
Size: 1012 5841
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6853
BstBI TT'CG_AA
Cuts at: 2007 5657 2007
Size: 3650 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6853
BstXI CCAn_nnnn'nTGG
Cuts at: 4132 4132
Size: 6853
BstYI r'GATC_y
Cuts at: 1972 3309 3330 3591 4183 4550 4935 4952 6404
Size: 1337 21 261 592 367 385 17 1452
Cuts at: 6404 6490 6502 6848 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1337 592 385 367 346 261
86 21 17 12
BstZ17I GTA'TAC
Cuts at: 3501 3501
Size: 6853
BtgI C'CryG_G
Cuts at: 513 1065 2022 2058 2204 2347 2475 3305 513
Size: 552 957 36 146 143 128 830 4061
Fragments arranged by size:
4061 957 830 552 146 143 128 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2795 487
Size: 683 56 1569 4545
Fragments arranged by size:
4545 1569 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 3895 4369 5163 5183 3643
Size: 252 474 794 20 5313
Fragments arranged by size:
5313 794 474 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2264 2299 529
Size: 35 1700 35 5083
Fragments arranged by size:
5083 1700 35 35
DraI TTT'AAA
Cuts at: 4157 4157
Size: 6853
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5860 809
Size: 507 4544 1802
Fragments arranged by size:
4544 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2272 2386
Size: 54 1425 50 275 18 377 65 114
Cuts at: 2386 3217 4134 4300 5189 6818 8
Size: 831 917 166 889 1629 43
Fragments arranged by size:
1629 1425 917 889 831 377 275 166
114 65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2207 4300 1537
Size: 275 395 2093 4090
Fragments arranged by size:
4090 2093 395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2059 2921 3399 3852 4783 1353
Size: 706 862 478 453 931 3423
Fragments arranged by size:
3423 931 862 706 478 453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 5440 5814 5960 387
Size: 1064 138 2501 1350 374 146 1280
Fragments arranged by size:
2501 1350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2655 2964 3058 3441 3669 3995
Size: 393 626 397 309 94 383 228 326
Cuts at: 3995 4916 5513 6300 6568 1239
Size: 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 626 597 397 393 383
326 309 268 228 94
EcoNI CCTnn'n_nnAGG
Cuts at: 2787 2787
Size: 6853
EcoO109I rG'GnC_Cy
Cuts at: 2133 2134 2480 3487 2133
Size: 1 346 1007 5499
Fragments arranged by size:
5499 1007 346 1
EcoRI G'AATT_C
Cuts at: 4162 4540 4162
Size: 378 6475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3392 3881 3392
Size: 489 6364
FspI TGC'GCA
Cuts at: 5359 5359
Size: 6853
HaeII r_GCGC'y
Cuts at: 1678 2443 6000 1678
Size: 765 3557 2531
Fragments arranged by size:
3557 2531 765
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2529 2561 3131 3163 3796
Size: 32 135 32 957 32 570 32 633
Cuts at: 3796 3828 5494 5526 5568 5600 1373
Size: 32 1666 32 42 32 2626
Fragments arranged by size:
2626 1666 957 633 570 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3681 4197 4443 669
Size: 988 281 1077 666 516 246 3079
Fragments arranged by size:
3079 1077 988 666 516 281 246
HindIII A'AGCT_T
Cuts at: 748 2587 4007 748
Size: 1839 1420 3594
Fragments arranged by size:
3594 1839 1420
HpaI GTT'AAC
Cuts at: 4443 4443
Size: 6853
KpnI G_GTAC'C
Cuts at: 4178 4178
Size: 6853
MfeI C'AATT_G
Cuts at: 3797 4452 3797
Size: 655 6198
MluI A'CGCG_T
Cuts at: 6729 6729
Size: 6853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3012 5967 6151 1305
Size: 1707 2955 184 2007
Fragments arranged by size:
2955 2007 1707 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5031
Fragments arranged by size:
5031 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2972 4811 5170 5329 518
Size: 648 1806 1839 359 159 2042
Fragments arranged by size:
2042 1839 1806 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2103 2197 2206 2258 2349 2477 3030
Size: 454 43 94 9 52 91 128 553
Cuts at: 3030 4269 4946 6094 6339 1606
Size: 1239 677 1148 245 2120
Fragments arranged by size:
2120 1239 1148 677 553 454 245 128
94 91 52 43 9
NaeI GCC'GGC
Cuts at: 2093 4088 2093
Size: 1995 4858
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5344
Fragments arranged by size:
5344 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6853
NgoMIV G'CCGG_C
Cuts at: 2091 4086 2091
Size: 1995 4858
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6853
NotI GC'GGCC_GC
Cuts at: 4300 4300
Size: 6853
NspI r_CATG'y
Cuts at: 1132 1229 4060 4211 5756 1132
Size: 97 2831 151 1545 2229
Fragments arranged by size:
2831 2229 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6853
PciI A'CATG_T
Cuts at: 5752 5752
Size: 6853
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6853
PmeI GTTT'AAAC
Cuts at: 4157 4157
Size: 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4922 4954 6435 6467 4922
Size: 32 1481 32 5308
Fragments arranged by size:
5308 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3487 3487
Size: 6853
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6853
PsiI TTA'TAA
Cuts at: 4423 4423
Size: 6853
PspOMI G'GGCC_C
Cuts at: 2133 2320 2361 2373 2480 2133
Size: 187 41 12 107 6506
Fragments arranged by size:
6506 187 107 41 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2706 2738 2825 2857 2706
Size: 32 87 32 6702
Fragments arranged by size:
6702 87 32 32
PstI C_TGCA'G
Cuts at: 830 2035 2190 4047 4205 830
Size: 1205 155 1857 158 3478
Fragments arranged by size:
3478 1857 1205 158 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6853
SacII CC_GC'GG
Cuts at: 2061 2207 2350 2478 2061
Size: 146 143 128 6436
Fragments arranged by size:
6436 146 143 128
SalI G'TCGA_C
Cuts at: 1936 4195 1936
Size: 2259 4594
SapI GCTCTTCn'nnn_
Cuts at: 2921 3852 2921
Size: 931 5922
SbfI CC_TGCA'GG
Cuts at: 4205 4205
Size: 6853
ScaI AGT'ACT
Cuts at: 2044 2724 5101 6813 2044
Size: 680 2377 1712 2084
Fragments arranged by size:
2377 2084 1712 680
SfcI C'TryA_G
Cuts at: 826 1045 2031 2186 2899 4043 4201 5336 6017
Size: 219 986 155 713 1144 158 1135 681
Cuts at: 6017 6208 826
Size: 191 1471
Fragments arranged by size:
1471 1144 1135 986 713 681 219 191
158 155
SfiI GGCCn_nnn'nGGCC
Cuts at: 2097 2097
Size: 6853
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6853
SmaI CCC'GGG
Cuts at: 1872 2325 4180 1872
Size: 453 1855 4545
Fragments arranged by size:
4545 1855 453
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2590 2787 4957 5858 6120 820
Size: 197 926 647 197 2170 901 262 1553
Fragments arranged by size:
2170 1553 926 901 647 262 197 197
SnaBI TAC'GTA
Cuts at: 493 4150 4644 493
Size: 3657 494 2702
Fragments arranged by size:
3657 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6853
SphI G_CATG'C
Cuts at: 1132 4211 1132
Size: 3079 3774
SspI AAT'ATT
Cuts at: 5 52 4777 5
Size: 47 4725 2081
Fragments arranged by size:
4725 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6853
StyI C'CwwG_G
Cuts at: 513 1065 2022 2877 2961 3090 4292 513
Size: 552 957 855 84 129 1202 3074
Fragments arranged by size:
3074 1202 957 855 552 129 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3761 4044 4880 4897 5050 5235 5680
Size: 1255 2181 283 836 17 153 185 445
Cuts at: 5680 325
Size: 1498
Fragments arranged by size:
2181 1498 1255 836 445 283 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2042 2722 3191
Size: 274 80 33 51 1443 65 680 469
Cuts at: 3191 5099 6811 96
Size: 1908 1712 138
Fragments arranged by size:
1908 1712 1443 680 469 274 138 80
65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6813 6845 6813
Size: 32 6821
Tth111I GACn'n_nGTC
Cuts at: 1316 3983 1316
Size: 2667 4186
XbaI T'CTAG_A
Cuts at: 4189 4189
Size: 6853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3958 3958
Size: 6853
XhoI C'TCGA_G
Cuts at: 1943 2787 1943
Size: 844 6009
XmaI C'CCGG_G
Cuts at: 1870 2323 4178 1870
Size: 453 1855 4545
Fragments arranged by size:
4545 1855 453
XmnI GAAnn'nnTTC
Cuts at: 4982 4982
Size: 6853
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4661 276
Size: 53 83 186 936 3127 2468
Fragments arranged by size:
3127 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PvuII RsrII SacI SanDI SexAI SfoI SrfI SwaI