Restriction map for
pFN21ASDA1991
(Linear) (Six-base) MAPSORT of: KIAA1991.seq Check: 3870 from: 1 to: 2124
KIAA1991.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 1044 1477 2124
Size: 1044 433 647
Fragments arranged by size:
1044 647 433
AclI AA'CG_TT
Cuts at: 0 720 2124
Size: 720 1404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1035 1418 1972 2124
Size: 632 403 383 554 152
Fragments arranged by size:
632 554 403 383 152
AfeI AGC'GCT
Cuts at: 0 418 2124
Size: 418 1706
ApaI G_GGCC'C
Cuts at: 0 114 301 342 354 461 2124
Size: 114 187 41 12 107 1663
Fragments arranged by size:
1663 187 114 107 41 12
AvaI C'yCGr_G
Cuts at: 0 23 300 412 764 2124
Size: 23 277 112 352 1360
Fragments arranged by size:
1360 352 277 112 23
BamHI G'GATC_C
Cuts at: 0 1286 2124
Size: 1286 838
BanI G'GyrC_C
Cuts at: 0 209 2124
Size: 209 1915
BanII G_rGCy'C
Cuts at: 0 114 221 301 342 354 461 2124
Size: 114 107 80 41 12 107 1663
Fragments arranged by size:
1663 114 107 107 80 41 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 1786 2009 2124
Size: 1786 223 115
Fragments arranged by size:
1786 223 115
BciVI GTATCCnnnnn_n'
Cuts at: 0 636 1414 2124
Size: 636 778 710
Fragments arranged by size:
778 710 636
BclI T'GATC_A
Cuts at: 0 1503 2124
Size: 1503 621
BglI GCCn_nnn'nGGC
Cuts at: 0 74 183 292 2124
Size: 74 109 109 1832
Fragments arranged by size:
1832 109 109 74
BglII A'GATC_T
Cuts at: 0 1307 2124
Size: 1307 817
Bme1580I G_kGCm'C
Cuts at: 0 114 301 342 354 461 2124
Size: 114 187 41 12 107 1663
Fragments arranged by size:
1663 187 114 107 41 12
BmgBI CAC'GTC
Cuts at: 0 733 2124
Size: 733 1391
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 1954 2124
Size: 116 1838 170
Fragments arranged by size:
1838 170 116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 941 1646 2124
Size: 235 706 705 478
Fragments arranged by size:
706 705 478 235
Bpu10I CC'TnA_GC
Cuts at: 0 825 2124
Size: 825 1299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 2124
Size: 588 1536
BsaBI GATnn'nnATC
Cuts at: 0 1502 2087 2124
Size: 1502 585 37
Fragments arranged by size:
1502 585 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 939 969 1108 1138 2124
Size: 805 30 104 30 139 30 986
Fragments arranged by size:
986 805 139 104 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1163 2124
Size: 1163 961
BseYI C'CCAG_C
Cuts at: 0 335 384 2124
Size: 335 49 1740
Fragments arranged by size:
1740 335 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 2124
Size: 713 1411
BsiEI CG_ry'CG
Cuts at: 0 187 2124
Size: 187 1937
BsiHKAI G_wGCw'C
Cuts at: 0 519 2124
Size: 519 1605
BsmBI CGTCTCn'nnnn_
Cuts at: 0 82 739 1017 2124
Size: 82 657 278 1107
Fragments arranged by size:
1107 657 278 82
Bsp1286I G_dGCh'C
Cuts at: 0 114 221 301 342 354 461 519 2124
Size: 114 107 80 41 12 107 58 1605
Fragments arranged by size:
1605 114 107 107 80 58 41 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1 1452 1591 2124
Size: 1 1451 139 533
Fragments arranged by size:
1451 533 139 1
BsrBI CCG'CTC
Cuts at: 0 52 1320 2124
Size: 52 1268 804
Fragments arranged by size:
1268 804 52
BsrDI GCAATG_nn'
Cuts at: 0 1119 2124
Size: 1119 1005
BsrFI r'CCGG_y
Cuts at: 0 68 2063 2124
Size: 68 1995 61
Fragments arranged by size:
1995 68 61
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2109 2124
Size: 2109 15
BstYI r'GATC_y
Cuts at: 0 1286 1307 1568 2124
Size: 1286 21 261 556
Fragments arranged by size:
1286 556 261 21
BstZ17I GTA'TAC
Cuts at: 0 1478 2124
Size: 1478 646
BtgI C'CryG_G
Cuts at: 0 35 181 324 452 1282 2124
Size: 35 146 143 128 830 842
Fragments arranged by size:
842 830 146 143 128 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 772 2124
Size: 772 1352
BtsI GCAGTG_nn'
Cuts at: 0 1620 1872 2124
Size: 1620 252 252
Fragments arranged by size:
1620 252 252
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 241 276 2124
Size: 241 35 1848
Fragments arranged by size:
1848 241 35
EaeI y'GGCC_r
Cuts at: 0 184 249 363 1194 2111 2124
Size: 184 65 114 831 917 13
Fragments arranged by size:
917 831 184 114 65 13
EagI C'GGCC_G
Cuts at: 0 184 2124
Size: 184 1940
EarI CTCTTCn'nnn_
Cuts at: 0 36 898 1376 1829 2124
Size: 36 862 478 453 295
Fragments arranged by size:
862 478 453 295 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2067 2124
Size: 2067 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 632 941 1035 1418 1646 1972 2124
Size: 235 397 309 94 383 228 326 152
Fragments arranged by size:
397 383 326 309 235 228 152 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 764 2124
Size: 764 1360
EcoO109I rG'GnC_Cy
Cuts at: 0 110 111 457 1464 2124
Size: 110 1 346 1007 660
Fragments arranged by size:
1007 660 346 110 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1369 1858 2124
Size: 1369 489 266
Fragments arranged by size:
1369 489 266
HaeII r_GCGC'y
Cuts at: 0 420 2124
Size: 420 1704
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 506 538 1108 1140 1773 1805 2124
Size: 506 32 570 32 633 32 319
Fragments arranged by size:
633 570 506 319 32 32 32
HincII GTy'rAC
Cuts at: 0 992 1658 2124
Size: 992 666 466
Fragments arranged by size:
992 666 466
HindIII A'AGCT_T
Cuts at: 0 564 1984 2124
Size: 564 1420 140
Fragments arranged by size:
1420 564 140
MfeI C'AATT_G
Cuts at: 0 1774 2124
Size: 1774 350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 2124
Size: 989 1135
MslI CAynn'nnrTG
Cuts at: 0 949 2124
Size: 949 1175
MspA1I CmG'CkG
Cuts at: 0 37 80 174 183 235 326 454 1007
Size: 37 43 94 9 52 91 128 553
Cuts at: 1007 2124
Size: 1117
Fragments arranged by size:
1117 553 128 94 91 52 43 37
9
NaeI GCC'GGC
Cuts at: 0 70 2065 2124
Size: 70 1995 59
Fragments arranged by size:
1995 70 59
NgoMIV G'CCGG_C
Cuts at: 0 68 2063 2124
Size: 68 1995 61
Fragments arranged by size:
1995 68 61
NspI r_CATG'y
Cuts at: 0 2037 2124
Size: 2037 87
PpuMI rG'GwC_Cy
Cuts at: 0 1464 2124
Size: 1464 660
PspOMI G'GGCC_C
Cuts at: 0 110 297 338 350 457 2124
Size: 110 187 41 12 107 1667
Fragments arranged by size:
1667 187 110 107 41 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 683 715 802 834 2124
Size: 683 32 87 32 1290
Fragments arranged by size:
1290 683 87 32 32
PstI C_TGCA'G
Cuts at: 0 12 167 2024 2124
Size: 12 155 1857 100
Fragments arranged by size:
1857 155 100 12
SacII CC_GC'GG
Cuts at: 0 38 184 327 455 2124
Size: 38 146 143 128 1669
Fragments arranged by size:
1669 146 143 128 38
SapI GCTCTTCn'nnn_
Cuts at: 0 898 1829 2124
Size: 898 931 295
Fragments arranged by size:
931 898 295
ScaI AGT'ACT
Cuts at: 0 21 701 2124
Size: 21 680 1423
Fragments arranged by size:
1423 680 21
SfcI C'TryA_G
Cuts at: 0 8 163 876 2020 2124
Size: 8 155 713 1144 104
Fragments arranged by size:
1144 713 155 104 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 74 2124
Size: 74 2050
SmaI CCC'GGG
Cuts at: 0 302 2124
Size: 302 1822
SmlI C'TyrA_G
Cuts at: 0 567 764 2124
Size: 567 197 1360
Fragments arranged by size:
1360 567 197
StyI C'CwwG_G
Cuts at: 0 854 938 1067 2124
Size: 854 84 129 1057
Fragments arranged by size:
1057 854 129 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1738 2021 2124
Size: 1738 283 103
Fragments arranged by size:
1738 283 103
TatI w'GTAC_w
Cuts at: 0 19 699 1168 2124
Size: 19 680 469 956
Fragments arranged by size:
956 680 469 19
Tth111I GACn'n_nGTC
Cuts at: 0 1960 2124
Size: 1960 164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1935 2124
Size: 1935 189
XhoI C'TCGA_G
Cuts at: 0 764 2124
Size: 764 1360
XmaI C'CCGG_G
Cuts at: 0 300 2124
Size: 300 1824
Enzymes that do cut:
AccI AclI AcuI AfeI ApaI AvaI BamHI BanI
BanII BbsI BciVI BclI BglI BglII Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I
NaeI NgoMIV NspI PpuMI PspOMI PsrI PstI SacII
SapI ScaI SfcI SfiI SmaI SmlI StyI TaqII
TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BfrBI BlpI BmtI
BplI BsaI BsaAI BsaHI BsaWI BsiWI BsmI BspEI
BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII Bsu36I
ClaI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI PvuII RsrII SacI SalI SanDI SbfI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XmnI ZraI