Restriction map for
pFN21ASDA1990
(Circular) (Six-base) MAPSORT of: pFN21ASDA1990.seq Check: 4158 from: 1 to: 7366
pFN21ASDA1990 7366 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3873 5176 278
Size: 53 83 186 936 2337 1303 2468
Fragments arranged by size:
2468 2337 1303 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4089 4709 1937
Size: 2152 620 4594
Fragments arranged by size:
4594 2152 620
Acc65I G'GTAC_C
Cuts at: 4687 4687
Size: 7366
AclI AA'CG_TT
Cuts at: 1563 3255 3277 5493 5866 1563
Size: 1692 22 2216 373 3063
Fragments arranged by size:
3063 2216 1692 373 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 3374 3579 5429 6813 7081 1632
Size: 830 912 205 1850 1384 268 1917
Fragments arranged by size:
1917 1850 1384 912 830 268 205
AflII C'TTAA_G
Cuts at: 820 1017 3827 820
Size: 197 2810 4359
Fragments arranged by size:
4359 2810 197
AflIII A'CryG_T
Cuts at: 1203 3597 6265 7242 1203
Size: 2394 2668 977 1327
Fragments arranged by size:
2668 2394 1327 977
AgeI A'CCGG_T
Cuts at: 2034 3380 6197 2034
Size: 1346 2817 3203
Fragments arranged by size:
3203 2817 1346
AhdI GACnn_n'nnGTC
Cuts at: 6095 6095
Size: 7366
AleI CACnn'nnGTG
Cuts at: 1166 2189 1166
Size: 1023 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3326 1184
Size: 2142 5224
AlwNI CAG_nnn'CTG
Cuts at: 2274 5109 6681 2274
Size: 2835 1572 2959
Fragments arranged by size:
2959 2835 1572
ApaLI G'TGCA_C
Cuts at: 2534 4472 5423 6579 2534
Size: 1938 951 1156 3321
Fragments arranged by size:
3321 1938 1156 951
ApoI r'AATT_y
Cuts at: 1493 2892 4675 4901 5053 7162 1493
Size: 1399 1783 226 152 2109 1697
Fragments arranged by size:
2109 1783 1697 1399 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5920 160
Size: 872 4888 1606
Fragments arranged by size:
4888 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
AvaI C'yCGr_G
Cuts at: 1870 1943 2564 3909 4239 4691 1870
Size: 73 621 1345 330 452 4545
Fragments arranged by size:
4545 1345 621 452 330 73
BamHI G'GATC_C
Cuts at: 2373 4011 4696 5063 2373
Size: 1638 685 367 4676
Fragments arranged by size:
4676 1638 685 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4687 6142 618
Size: 325 217 627 2900 1455 1842
Fragments arranged by size:
2900 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3289 4386 4685 1391
Size: 1898 1097 299 4072
Fragments arranged by size:
4072 1898 1097 299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2062 2574 3009 928
Size: 971 163 512 435 5285
Fragments arranged by size:
5285 971 512 435 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3122 3156 5557 5591 1903
Size: 34 1185 34 2401 34 3678
Fragments arranged by size:
3678 2401 1185 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2486 2804 4553 5258 6468 2486
Size: 318 1749 705 1210 3384
Fragments arranged by size:
3384 1749 1210 705 318
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7366
BfrBI ATG'CAT
Cuts at: 2557 2557
Size: 7366
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5977 136
Size: 107 122 71 631 605 4305 1525
Fragments arranged by size:
4305 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2637 7361 2637
Size: 4724 2642
BlpI GC'TnA_GC
Cuts at: 4783 4783
Size: 7366
Bme1580I G_kGCm'C
Cuts at: 1790 2186 2538 4476 5427 6583 1790
Size: 396 352 1938 951 1156 2573
Fragments arranged by size:
2573 1938 1156 951 396 352
BmgBI CAC'GTC
Cuts at: 1318 2109 2593 1318
Size: 791 484 6091
Fragments arranged by size:
6091 791 484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3016 3642 5097
Size: 416 53 83 399 24 1599 626 1455
Cuts at: 5097 6055 442
Size: 958 1753
Fragments arranged by size:
1753 1599 1455 958 626 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 7366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2712 3002 3089 3275 3884 3939 4466 6026
Size: 1473 290 87 186 609 55 527 1560
Cuts at: 6026 1239
Size: 2579
Fragments arranged by size:
2579 1560 1473 609 527 290 186 87
55
Bpu10I CC'TnA_GC
Cuts at: 2288 2288
Size: 7366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5491 6356 6654 5491
Size: 865 298 6203
Fragments arranged by size:
6203 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2693 2837 3092 6029 882
Size: 617 1194 144 255 2937 2219
Fragments arranged by size:
2937 2219 1194 617 255 144
BsaAI yAC'GTr
Cuts at: 493 1204 4663 5157 493
Size: 711 3459 494 2702
Fragments arranged by size:
3459 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4550 7360 4550
Size: 2810 4556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3870 4281 5173 5555
Size: 53 83 186 936 2337 411 892 382
Cuts at: 5555 6190 275
Size: 635 1451
Fragments arranged by size:
2337 1451 936 892 635 411 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2034 2646 3380 4128 4392 5799 6197 6471 6618
Size: 612 734 748 264 1407 398 274 147
Cuts at: 6618 7029 2034
Size: 411 2371
Fragments arranged by size:
2371 1407 748 734 612 411 398 274
264 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3653 3683 1178
Size: 30 165 30 2250 30 4861
Fragments arranged by size:
4861 2250 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 2795 3058 3216 3393 1186
Size: 1330 279 263 158 177 5159
Fragments arranged by size:
5159 1330 279 263 177 158
BseYI C'CCAG_C
Cuts at: 2764 3366 4136 6569 7065 2764
Size: 602 770 2433 496 3065
Fragments arranged by size:
3065 2433 770 602 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3167 1163
Size: 573 1431 5362
Fragments arranged by size:
5362 1431 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3415 4816 5577 6605 1540
Size: 275 186 1414 1401 761 1028 2301
Fragments arranged by size:
2301 1414 1401 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2294 2538 4476 5427 5512 6583 2294
Size: 244 1938 951 85 1071 3077
Fragments arranged by size:
3077 1938 1071 951 244 85
BsmI GAATG_Cn'
Cuts at: 2726 4138 4877 4970 2726
Size: 1412 739 93 5122
Fragments arranged by size:
5122 1412 739 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2294 2538 3289 4386 4476 4685
Size: 399 396 108 244 751 1097 90 209
Cuts at: 4685 5427 5512 6583 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1097 1071 751 742 399 396 244
209 108 90 85
BspEI T'CCGG_A
Cuts at: 4128 7029 4128
Size: 2901 4465
BspHI T'CATG_A
Cuts at: 3075 3783 5255 6985 3075
Size: 708 1472 1730 3456
Fragments arranged by size:
3456 1730 1472 708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3796 3864 4721 844
Size: 2952 68 857 3489
Fragments arranged by size:
3489 2952 857 68
BsrBI CCG'CTC
Cuts at: 1394 1823 5253 1394
Size: 429 3430 3507
Fragments arranged by size:
3507 3430 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5861 6035 65
Size: 1183 4613 174 1396
Fragments arranged by size:
4613 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 2785 3380 3414 6010 6197 1700
Size: 110 224 751 595 34 2596 187 2869
Fragments arranged by size:
2869 2596 751 595 224 187 110 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7366
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7366
BssSI C'ACGA_G
Cuts at: 5426 6438 5426
Size: 1012 6354
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2615 794
Size: 1821 5545
BstBI TT'CG_AA
Cuts at: 2007 6170 2007
Size: 4163 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7366
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 3442 3679 4219 2506
Size: 936 237 540 5653
Fragments arranged by size:
5653 936 540 237
BstYI r'GATC_y
Cuts at: 1972 2373 2637 4011 4696 5063 5448 5465 6917
Size: 401 264 1374 685 367 385 17 1452
Cuts at: 6917 7003 7015 7361 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1374 685 401 385 367 346
264 86 17 12
BstZ17I GTA'TAC
Cuts at: 4090 4090
Size: 7366
BtgI C'CryG_G
Cuts at: 513 1065 2130 513
Size: 552 1065 5749
Fragments arranged by size:
5749 1065 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4537 487
Size: 683 56 3311 3316
Fragments arranged by size:
3316 3311 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 2580 3150 4432 4882 5676 5696 2292
Size: 288 570 1282 450 794 20 3962
Fragments arranged by size:
3962 1282 794 570 450 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2856 2891 3709 3744 529
Size: 35 2292 35 818 35 4151
Fragments arranged by size:
4151 2292 818 35 35 35
DraI TTT'AAA
Cuts at: 4670 4670
Size: 7366
DraIII CAC_nnn'GTG
Cuts at: 3703 3703
Size: 7366
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3806 6373 809
Size: 507 2490 2567 1802
Fragments arranged by size:
2567 2490 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2508 2746 4111
Size: 54 1425 50 275 18 678 238 1365
Cuts at: 4111 4813 5702 7331 8
Size: 702 889 1629 43
Fragments arranged by size:
1629 1425 1365 889 702 678 275 238
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4813 1537
Size: 275 3001 4090
Fragments arranged by size:
4090 3001 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3671 4155 4494 4610 5296 1353
Size: 2318 484 339 116 686 3423
Fragments arranged by size:
3423 2318 686 484 339 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5953 6327 6473 387
Size: 1064 138 4364 374 146 1280
Fragments arranged by size:
4364 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 2712 3002 3089 3275 3374 3579
Size: 393 830 250 290 87 186 99 205
Cuts at: 3579 3884 3939 4466 5429 6026 6813 7081 1239
Size: 305 55 527 963 597 787 268 1524
Fragments arranged by size:
1524 963 830 787 597 527 393 305
290 268 250 205 186 99 87 55
EcoO109I rG'GnC_Cy
Cuts at: 3493 3493
Size: 7366
EcoRI G'AATT_C
Cuts at: 4675 5053 4675
Size: 378 6988
FspI TGC'GCA
Cuts at: 5872 5872
Size: 7366
HaeII r_GCGC'y
Cuts at: 1678 4453 4621 6513 1678
Size: 2775 168 1892 2531
Fragments arranged by size:
2775 2531 1892 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2160 2192 2636 2668 2977
Size: 32 135 32 588 32 444 32 309
Cuts at: 2977 3009 3334 3366 3587 3611 3619 3643 3892
Size: 32 325 32 221 24 8 24 249
Cuts at: 3892 3911 3924 3943 4041 4073 6007 6039 6081
Size: 19 13 19 98 32 1934 32 42
Cuts at: 6081 6113 1373
Size: 32 2626
Fragments arranged by size:
2626 1934 588 444 325 309 249 221
135 98 42 32 32 32 32 32
32 32 32 32 24 24 19 19
13 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2737 4710 4956 669
Size: 988 281 799 1973 246 3079
Fragments arranged by size:
3079 1973 988 799 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7366
HpaI GTT'AAC
Cuts at: 4956 4956
Size: 7366
KpnI G_GTAC'C
Cuts at: 4691 4691
Size: 7366
MfeI C'AATT_G
Cuts at: 4965 4965
Size: 7366
MluI A'CGCG_T
Cuts at: 7242 7242
Size: 7366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2032 2799 3818 6480 6664 1305
Size: 727 767 1019 2662 184 2007
Fragments arranged by size:
2662 2007 1019 767 727 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2748 4113 10
Size: 54 1425 343 916 1365 3263
Fragments arranged by size:
3263 1425 1365 916 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 3596 5324 5683 5842 518
Size: 648 1023 1407 1728 359 159 2042
Fragments arranged by size:
2042 1728 1407 1023 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 2588 2980 3366 4782 5459 6607 6852
Size: 508 474 392 386 1416 677 1148 245
Cuts at: 6852 1606
Size: 2120
Fragments arranged by size:
2120 1416 1148 677 508 474 392 386
245
NcoI C'CATG_G
Cuts at: 513 1065 2130 513
Size: 552 1065 5749
Fragments arranged by size:
5749 1065 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7366
NotI GC'GGCC_GC
Cuts at: 4813 4813
Size: 7366
NruI TCG'CGA
Cuts at: 4278 4278
Size: 7366
NsiI A_TGCA'T
Cuts at: 2559 2559
Size: 7366
NspI r_CATG'y
Cuts at: 1132 1229 2561 2807 3601 3718 3841 4088 4724
Size: 97 1332 246 794 117 123 247 636
Cuts at: 4724 6269 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1332 794 636 247 246 123
117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7366
PciI A'CATG_T
Cuts at: 3597 6265 3597
Size: 2668 4698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7366
PfoI T'CCnGG_A
Cuts at: 3861 4335 3861
Size: 474 6892
PmeI GTTT'AAAC
Cuts at: 4670 4670
Size: 7366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2075 2107 5435 5467 6948 6980 2075
Size: 32 3328 32 1481 32 2461
Fragments arranged by size:
3328 2461 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3493 3493
Size: 7366
PshAI GACnn'nnGTC
Cuts at: 1613 4234 1613
Size: 2621 4745
PsiI TTA'TAA
Cuts at: 4936 4936
Size: 7366
PspXI vC'TCGA_Gb
Cuts at: 3909 3909
Size: 7366
PstI C_TGCA'G
Cuts at: 830 2856 3145 3752 4293 4718 830
Size: 2026 289 607 541 425 3478
Fragments arranged by size:
3478 2026 607 541 425 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7366
PvuII CAG'CTG
Cuts at: 2114 2980 2114
Size: 866 6500
SalI G'TCGA_C
Cuts at: 1936 4708 1936
Size: 2772 4594
SapI GCTCTTCn'nnn_
Cuts at: 4155 4494 4155
Size: 339 7027
SbfI CC_TGCA'GG
Cuts at: 4718 4718
Size: 7366
ScaI AGT'ACT
Cuts at: 3473 5614 7326 3473
Size: 2141 1712 3513
Fragments arranged by size:
3513 2141 1712
SexAI A'CCwGG_T
Cuts at: 2267 2541 2267
Size: 274 7092
SfcI C'TryA_G
Cuts at: 826 1045 2852 2996 3141 3656 3748 4289 4432
Size: 219 1807 144 145 515 92 541 143
Cuts at: 4432 4438 4714 5849 6530 6721 826
Size: 6 276 1135 681 191 1471
Fragments arranged by size:
1807 1471 1135 681 541 515 276 219
191 145 144 143 92 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7366
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7366
SmaI CCC'GGG
Cuts at: 1872 4693 1872
Size: 2821 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3827 3909 5470 6371 6633 820
Size: 197 926 1884 82 1561 901 262 1553
Fragments arranged by size:
1884 1561 1553 926 901 262 197 82
SnaBI TAC'GTA
Cuts at: 493 4663 5157 493
Size: 4170 494 2702
Fragments arranged by size:
4170 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7366
SphI G_CATG'C
Cuts at: 1132 3718 4724 1132
Size: 2586 1006 3774
Fragments arranged by size:
3774 2586 1006
SspI AAT'ATT
Cuts at: 5 52 5290 5
Size: 47 5238 2081
Fragments arranged by size:
5238 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7366
StyI C'CwwG_G
Cuts at: 513 1065 2130 2838 4370 4575 4805 513
Size: 552 1065 708 1532 205 230 3074
Fragments arranged by size:
3074 1532 1065 708 552 230 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2442 2717 4625 5393 5410 5563
Size: 1255 579 283 275 1908 768 17 153
Cuts at: 5563 5748 6193 325
Size: 185 445 1498
Fragments arranged by size:
1908 1498 1255 768 579 445 283 275
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3471 3882 5612
Size: 274 80 33 51 1443 1494 411 1730
Cuts at: 5612 7324 96
Size: 1712 138
Fragments arranged by size:
1730 1712 1494 1443 411 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 7326 7358 3569
Size: 32 3725 32 3577
Fragments arranged by size:
3725 3577 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7366
XbaI T'CTAG_A
Cuts at: 4702 4702
Size: 7366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4333 4333
Size: 7366
XhoI C'TCGA_G
Cuts at: 1943 3909 1943
Size: 1966 5400
XmaI C'CCGG_G
Cuts at: 1870 4691 1870
Size: 2821 4545
XmnI GAAnn'nnTTC
Cuts at: 5495 5495
Size: 7366
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3871 5174 276
Size: 53 83 186 936 2337 1303 2468
Fragments arranged by size:
2468 2337 1303 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV PacI PmlI
PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI