Restriction map for
pFN21ASDA1990
(Linear) (Six-base) MAPSORT of: KIAA1990.seq Check: 1586 from: 1 to: 2637
KIAA1990.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AatII G_ACGT'C
Cuts at: 0 1850 2637
Size: 1850 787
AccI GT'mk_AC
Cuts at: 0 2066 2637
Size: 2066 571
AclI AA'CG_TT
Cuts at: 0 1232 1254 2637
Size: 1232 22 1383
Fragments arranged by size:
1383 1232 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1351 1556 2637
Size: 439 912 205 1081
Fragments arranged by size:
1081 912 439 205
AflII C'TTAA_G
Cuts at: 0 1804 2637
Size: 1804 833
AflIII A'CryG_T
Cuts at: 0 1574 2637
Size: 1574 1063
AgeI A'CCGG_T
Cuts at: 0 11 1357 2637
Size: 11 1346 1280
Fragments arranged by size:
1346 1280 11
AleI CACnn'nnGTG
Cuts at: 0 166 2637
Size: 166 2471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1303 2637
Size: 1303 1334
AlwNI CAG_nnn'CTG
Cuts at: 0 251 2637
Size: 251 2386
ApaLI G'TGCA_C
Cuts at: 0 511 2449 2637
Size: 511 1938 188
Fragments arranged by size:
1938 511 188
ApoI r'AATT_y
Cuts at: 0 869 2637
Size: 869 1768
AvaI C'yCGr_G
Cuts at: 0 541 1886 2216 2637
Size: 541 1345 330 421
Fragments arranged by size:
1345 541 421 330
BamHI G'GATC_C
Cuts at: 0 350 1988 2637
Size: 350 1638 649
Fragments arranged by size:
1638 649 350
BanII G_rGCy'C
Cuts at: 0 1266 2363 2637
Size: 1266 1097 274
Fragments arranged by size:
1266 1097 274
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 551 986 2637
Size: 39 512 435 1651
Fragments arranged by size:
1651 512 435 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1099 1133 2637
Size: 1099 34 1504
Fragments arranged by size:
1504 1099 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 463 781 2530 2637
Size: 463 318 1749 107
Fragments arranged by size:
1749 463 318 107
BfrBI ATG'CAT
Cuts at: 0 534 2637
Size: 534 2103
BglII A'GATC_T
Cuts at: 0 614 2637
Size: 614 2023
Bme1580I G_kGCm'C
Cuts at: 0 163 515 2453 2637
Size: 163 352 1938 184
Fragments arranged by size:
1938 352 184 163
BmgBI CAC'GTC
Cuts at: 0 86 570 2637
Size: 86 484 2067
Fragments arranged by size:
2067 484 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 993 1619 2637
Size: 993 626 1018
Fragments arranged by size:
1018 993 626
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 486 2637
Size: 486 2151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 979 1066 1252 1861 1916 2443 2637
Size: 689 290 87 186 609 55 527 194
Fragments arranged by size:
689 609 527 290 194 186 87 55
Bpu10I CC'TnA_GC
Cuts at: 0 265 2637
Size: 265 2372
BsaI GGTCTCn'nnnn_
Cuts at: 0 670 814 1069 2637
Size: 670 144 255 1568
Fragments arranged by size:
1568 670 255 144
BsaBI GATnn'nnATC
Cuts at: 0 2527 2637
Size: 2527 110
BsaHI Gr'CG_yC
Cuts at: 0 1847 2258 2637
Size: 1847 411 379
Fragments arranged by size:
1847 411 379
BsaWI w'CCGG_w
Cuts at: 0 11 623 1357 2105 2369 2637
Size: 11 612 734 748 264 268
Fragments arranged by size:
748 734 612 268 264 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1630 1660 2637
Size: 1630 30 977
Fragments arranged by size:
1630 977 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 772 1035 1193 1370 2637
Size: 493 279 263 158 177 1267
Fragments arranged by size:
1267 493 279 263 177 158
BseYI C'CCAG_C
Cuts at: 0 741 1343 2113 2637
Size: 741 602 770 524
Fragments arranged by size:
770 741 602 524
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 2637
Size: 1144 1493
BsiEI CG_ry'CG
Cuts at: 0 1392 2637
Size: 1392 1245
BsiHKAI G_wGCw'C
Cuts at: 0 271 515 2453 2637
Size: 271 244 1938 184
Fragments arranged by size:
1938 271 244 184
BsmI GAATG_Cn'
Cuts at: 0 703 2115 2637
Size: 703 1412 522
Fragments arranged by size:
1412 703 522
Bsp1286I G_dGCh'C
Cuts at: 0 163 271 515 1266 2363 2453 2637
Size: 163 108 244 751 1097 90 184
Fragments arranged by size:
1097 751 244 184 163 108 90
BspEI T'CCGG_A
Cuts at: 0 2105 2637
Size: 2105 532
BspHI T'CATG_A
Cuts at: 0 1052 1760 2637
Size: 1052 708 877
Fragments arranged by size:
1052 877 708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1773 1841 2637
Size: 1773 68 796
Fragments arranged by size:
1773 796 68
BsrFI r'CCGG_y
Cuts at: 0 11 762 1357 1391 2637
Size: 11 751 595 34 1246
Fragments arranged by size:
1246 751 595 34 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 592 2637
Size: 592 2045
BstXI CCAn_nnnn'nTGG
Cuts at: 0 483 1419 1656 2196 2637
Size: 483 936 237 540 441
Fragments arranged by size:
936 540 483 441 237
BstYI r'GATC_y
Cuts at: 0 350 614 1988 2637
Size: 350 264 1374 649
Fragments arranged by size:
1374 649 350 264
BstZ17I GTA'TAC
Cuts at: 0 2067 2637
Size: 2067 570
BtgI C'CryG_G
Cuts at: 0 107 2637
Size: 107 2530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2514 2637
Size: 2514 123
BtsI GCAGTG_nn'
Cuts at: 0 269 557 1127 2409 2637
Size: 269 288 570 1282 228
Fragments arranged by size:
1282 570 288 269 228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 833 868 1686 1721 2637
Size: 833 35 818 35 916
Fragments arranged by size:
916 833 818 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1680 2637
Size: 1680 957
DrdI GACnn_nn'nnGTC
Cuts at: 0 1783 2637
Size: 1783 854
EaeI y'GGCC_r
Cuts at: 0 485 723 2088 2637
Size: 485 238 1365 549
Fragments arranged by size:
1365 549 485 238
EarI CTCTTCn'nnn_
Cuts at: 0 1648 2132 2471 2587 2637
Size: 1648 484 339 116 50
Fragments arranged by size:
1648 484 339 116 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 689 979 1066 1252 1351 1556 1861
Size: 439 250 290 87 186 99 205 305
Cuts at: 1861 1916 2443 2637
Size: 55 527 194
Fragments arranged by size:
527 439 305 290 250 205 194 186
99 87 55
EcoO109I rG'GnC_Cy
Cuts at: 0 1470 2637
Size: 1470 1167
HaeII r_GCGC'y
Cuts at: 0 2430 2598 2637
Size: 2430 168 39
Fragments arranged by size:
2430 168 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 613 645 954 986 1311 1343
Size: 137 32 444 32 309 32 325 32
Cuts at: 1343 1564 1588 1596 1620 1869 1888 1901 1920
Size: 221 24 8 24 249 19 13 19
Cuts at: 1920 2018 2050 2637
Size: 98 32 587
Fragments arranged by size:
587 444 325 309 249 221 137 98
32 32 32 32 32 24 24 19
19 13 8
HincII GTy'rAC
Cuts at: 0 714 2637
Size: 714 1923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 776 1795 2637
Size: 9 767 1019 842
Fragments arranged by size:
1019 842 767 9
MscI TGG'CCA
Cuts at: 0 725 2090 2637
Size: 725 1365 547
Fragments arranged by size:
1365 725 547
MslI CAynn'nnrTG
Cuts at: 0 166 1573 2637
Size: 166 1407 1064
Fragments arranged by size:
1407 1064 166
MspA1I CmG'CkG
Cuts at: 0 91 565 957 1343 2637
Size: 91 474 392 386 1294
Fragments arranged by size:
1294 474 392 386 91
NcoI C'CATG_G
Cuts at: 0 107 2637
Size: 107 2530
NruI TCG'CGA
Cuts at: 0 2255 2637
Size: 2255 382
NsiI A_TGCA'T
Cuts at: 0 536 2637
Size: 536 2101
NspI r_CATG'y
Cuts at: 0 538 784 1578 1695 1818 2065 2637
Size: 538 246 794 117 123 247 572
Fragments arranged by size:
794 572 538 247 246 123 117
PciI A'CATG_T
Cuts at: 0 1574 2637
Size: 1574 1063
PfoI T'CCnGG_A
Cuts at: 0 1838 2312 2637
Size: 1838 474 325
Fragments arranged by size:
1838 474 325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 2637
Size: 52 32 2553
Fragments arranged by size:
2553 52 32
PpuMI rG'GwC_Cy
Cuts at: 0 1470 2637
Size: 1470 1167
PshAI GACnn'nnGTC
Cuts at: 0 2211 2637
Size: 2211 426
PspXI vC'TCGA_Gb
Cuts at: 0 1886 2637
Size: 1886 751
PstI C_TGCA'G
Cuts at: 0 833 1122 1729 2270 2637
Size: 833 289 607 541 367
Fragments arranged by size:
833 607 541 367 289
PvuII CAG'CTG
Cuts at: 0 91 957 2637
Size: 91 866 1680
Fragments arranged by size:
1680 866 91
SapI GCTCTTCn'nnn_
Cuts at: 0 2132 2471 2637
Size: 2132 339 166
Fragments arranged by size:
2132 339 166
ScaI AGT'ACT
Cuts at: 0 1450 2637
Size: 1450 1187
SexAI A'CCwGG_T
Cuts at: 0 244 518 2637
Size: 244 274 2119
Fragments arranged by size:
2119 274 244
SfcI C'TryA_G
Cuts at: 0 829 973 1118 1633 1725 2266 2409 2415
Size: 829 144 145 515 92 541 143 6
Cuts at: 2415 2637
Size: 222
Fragments arranged by size:
829 541 515 222 145 144 143 92
6
SmlI C'TyrA_G
Cuts at: 0 1804 1886 2637
Size: 1804 82 751
Fragments arranged by size:
1804 751 82
SphI G_CATG'C
Cuts at: 0 1695 2637
Size: 1695 942
StyI C'CwwG_G
Cuts at: 0 107 815 2347 2552 2637
Size: 107 708 1532 205 85
Fragments arranged by size:
1532 708 205 107 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 419 694 2602 2637
Size: 136 283 275 1908 35
Fragments arranged by size:
1908 283 275 136 35
TatI w'GTAC_w
Cuts at: 0 1448 1859 2637
Size: 1448 411 778
Fragments arranged by size:
1448 778 411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1546 1578 2637
Size: 1546 32 1059
Fragments arranged by size:
1546 1059 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2310 2637
Size: 2310 327
XhoI C'TCGA_G
Cuts at: 0 1886 2637
Size: 1886 751
ZraI GAC'GTC
Cuts at: 0 1848 2637
Size: 1848 789
Enzymes that do cut:
AatII AccI AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AvaI BamHI BanII BbsI
BcgI BciVI BfrBI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrFI BstAPI BstXI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI Eco57MI EcoO109I
HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI
NruI NsiI NspI PciI PfoI PpiI PpuMI PshAI
PspXI PstI PvuII SapI ScaI SexAI SfcI SmlI
SphI StyI TaqII TatI TstI XcmI XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AhdI AloI ApaI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BclI BglI
BlpI BmtI BpuEI BsaAI BsiWI BsmBI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII Bsu36I ClaI DraI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI PacI PasI
PflMI PmeI PmlI PsiI PspOMI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XmaI XmnI