(Circular) (Six-base) MAPSORT of: pFN21ASDA1971.seq Check: 2872 from: 1 to: 7156 pFN21ASDA1971 7156 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4108 4108 Size: 7156 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4966 278 Size: 53 83 186 936 3430 2468 Fragments arranged by size: 3430 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4499 1937 Size: 2562 4594 Acc65I G'GTAC_C Cuts at: 4477 4477 Size: 7156 AclI AA'CG_TT Cuts at: 1563 2679 5283 5656 1563 Size: 1116 2604 373 3063 Fragments arranged by size: 3063 2604 1116 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2886 2907 3023 3543 4301 5219 6603 6871 Size: 1254 21 116 520 758 918 1384 268 Cuts at: 6871 1632 Size: 1917 Fragments arranged by size: 1917 1384 1254 918 758 520 268 116 21 AfeI AGC'GCT Cuts at: 2330 2330 Size: 7156 AflII C'TTAA_G Cuts at: 820 1017 4171 820 Size: 197 3154 3805 Fragments arranged by size: 3805 3154 197 AflIII A'CryG_T Cuts at: 1203 6055 7032 1203 Size: 4852 977 1327 Fragments arranged by size: 4852 1327 977 AgeI A'CCGG_T Cuts at: 5987 5987 Size: 7156 AhdI GACnn_n'nnGTC Cuts at: 5885 5885 Size: 7156 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7156 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 2126 Size: 32 7124 AlwNI CAG_nnn'CTG Cuts at: 2455 3424 3447 3707 4899 6471 2455 Size: 969 23 260 1192 1572 3140 Fragments arranged by size: 3140 1572 1192 969 260 23 ApaI G_GGCC'C Cuts at: 2200 2211 2200 Size: 11 7145 ApaLI G'TGCA_C Cuts at: 5213 6369 5213 Size: 1156 6000 ApoI r'AATT_y Cuts at: 1493 3114 3195 3221 3385 4465 4691 4843 6952 Size: 1621 81 26 164 1080 226 152 2109 Cuts at: 6952 1493 Size: 1697 Fragments arranged by size: 2109 1697 1621 1080 226 164 152 81 26 AseI AT'TA_AT Cuts at: 160 1032 2672 5710 160 Size: 872 1640 3038 1606 Fragments arranged by size: 3038 1640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7156 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2210 2216 2393 3054 4481 1870 Size: 73 147 120 6 177 661 1427 4545 Fragments arranged by size: 4545 1427 661 177 147 120 73 6 AvrII C'CTAG_G Cuts at: 2827 2827 Size: 7156 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2149 2182 3529 3562 2149 Size: 33 1347 33 5743 Fragments arranged by size: 5743 1347 33 33 BamHI G'GATC_C Cuts at: 4486 4853 4486 Size: 367 6789 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2097 2507 4477 5932 Size: 325 217 627 272 38 410 1970 1455 Cuts at: 5932 618 Size: 1842 Fragments arranged by size: 1970 1842 1455 627 410 325 272 217 38 BanII G_rGCy'C Cuts at: 1391 2091 2200 2211 2217 2223 2283 2394 4057 Size: 700 109 11 6 6 60 111 1663 Cuts at: 4057 4189 4475 1391 Size: 132 286 4072 Fragments arranged by size: 4072 1663 700 286 132 111 109 60 11 6 6 BbeI G_GCGC'C Cuts at: 2511 2511 Size: 7156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6185 BbvCI CC'TCA_GC Cuts at: 2538 2538 Size: 7156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4082 4116 5347 5381 1903 Size: 34 2145 34 1231 34 3678 Fragments arranged by size: 3678 2145 1231 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5048 6258 5048 Size: 1210 5946 BclI T'GATC_A Cuts at: 1548 3898 1548 Size: 2350 4806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 2095 5767 Size: 107 122 71 631 605 380 43 3672 Cuts at: 5767 136 Size: 1525 Fragments arranged by size: 3672 1525 631 605 380 122 107 71 43 BglII A'GATC_T Cuts at: 3133 7151 3133 Size: 4018 3138 BlpI GC'TnA_GC Cuts at: 4104 4573 4104 Size: 469 6687 Bme1580I G_kGCm'C Cuts at: 1790 2200 2211 5217 6373 1790 Size: 410 11 3006 1156 2573 Fragments arranged by size: 3006 2573 1156 410 11 BmgBI CAC'GTC Cuts at: 1318 2362 4079 1318 Size: 1044 1717 4395 Fragments arranged by size: 4395 1717 1044 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4887 5845 442 Size: 416 53 83 399 24 3470 958 1753 Fragments arranged by size: 3470 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3053 1056 Size: 1997 5159 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 4208 3482 Size: 726 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2345 2873 3446 4041 4361 5816 1239 Size: 830 276 528 573 595 320 1455 2579 Fragments arranged by size: 2579 1455 830 595 573 528 320 276 Bpu10I CC'TnA_GC Cuts at: 2538 2538 Size: 7156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4005 4373 5281 6146 6444 4005 Size: 368 908 865 298 4717 Fragments arranged by size: 4717 908 865 368 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5819 882 Size: 617 4320 2219 Fragments arranged by size: 4320 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4115 4453 4947 493 Size: 711 2911 338 494 2702 Fragments arranged by size: 2911 2702 711 494 338 BsaBI GATnn'nnATC Cuts at: 3649 7150 3649 Size: 3501 3655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2508 4963 5345 5980 Size: 53 83 186 936 975 2455 382 635 Cuts at: 5980 275 Size: 1451 Fragments arranged by size: 2455 1451 975 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3468 4194 5589 5987 6261 6408 6819 3468 Size: 726 1395 398 274 147 411 3805 Fragments arranged by size: 3805 1395 726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 1178 Size: 30 165 30 723 30 6178 Fragments arranged by size: 6178 723 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3605 3936 3939 3942 3984 4179 4372 4424 Size: 2419 331 3 3 42 195 193 52 Cuts at: 4424 1186 Size: 3918 Fragments arranged by size: 3918 2419 331 195 193 52 42 3 3 BseYI C'CCAG_C Cuts at: 2055 2253 3738 4313 6359 6855 2055 Size: 198 1485 575 2046 496 2356 Fragments arranged by size: 2356 2046 1485 575 496 198 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6583 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2164 2274 2493 2553 4606 5367 Size: 275 186 163 110 219 60 2053 761 Cuts at: 5367 6395 1540 Size: 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 219 186 163 110 60 BsiHKAI G_wGCw'C Cuts at: 4006 4189 4248 5217 5302 6373 4006 Size: 183 59 969 85 1071 4789 Fragments arranged by size: 4789 1071 969 183 85 59 BsmI GAATG_Cn' Cuts at: 2964 4667 4760 2964 Size: 1703 93 5360 Fragments arranged by size: 5360 1703 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2245 2905 2245 Size: 660 6496 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2200 2211 2217 2223 2283 2394 Size: 399 301 109 11 6 6 60 111 Cuts at: 2394 4006 4057 4189 4248 4475 5217 5302 6373 Size: 1612 51 132 59 227 742 85 1071 Cuts at: 6373 1391 Size: 2174 Fragments arranged by size: 2174 1612 1071 742 399 301 227 132 111 109 85 60 59 51 11 6 6 BspEI T'CCGG_A Cuts at: 3468 4194 6819 3468 Size: 726 2625 3805 Fragments arranged by size: 3805 2625 726 BspHI T'CATG_A Cuts at: 3072 5045 6775 3072 Size: 1973 1730 3453 Fragments arranged by size: 3453 1973 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3729 4108 4511 844 Size: 2885 379 403 3489 Fragments arranged by size: 3489 2885 403 379 BsrBI CCG'CTC Cuts at: 1394 1823 2584 5043 1394 Size: 429 761 2459 3507 Fragments arranged by size: 3507 2459 761 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5651 5825 65 Size: 1183 4403 174 1396 Fragments arranged by size: 4403 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2100 2258 2295 4210 5800 5987 Size: 110 252 38 158 37 1915 1590 187 Cuts at: 5987 1700 Size: 2869 Fragments arranged by size: 2869 1915 1590 252 187 158 110 38 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7156 BssHII G'CGCG_C Cuts at: 1925 2289 2422 2593 2595 1925 Size: 364 133 171 2 6486 Fragments arranged by size: 6486 364 171 133 2 BssSI C'ACGA_G Cuts at: 5216 6228 5216 Size: 1012 6144 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 794 Size: 1310 5846 BstBI TT'CG_AA Cuts at: 2007 5960 2007 Size: 3953 3203 BstEII G'GTnAC_C Cuts at: 1184 2716 1184 Size: 1532 5624 BstXI CCAn_nnnn'nTGG Cuts at: 2662 4445 2662 Size: 1783 5373 BstYI r'GATC_y Cuts at: 1972 3133 3444 4145 4486 4853 5238 5255 6707 Size: 1161 311 701 341 367 385 17 1452 Cuts at: 6707 6793 6805 7151 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1161 701 385 367 346 341 311 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2229 2229 Size: 7156 BtgI C'CryG_G Cuts at: 513 1065 2022 2274 2284 2339 2661 2721 513 Size: 552 957 252 10 55 322 60 4948 Fragments arranged by size: 4948 957 552 322 252 60 55 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6417 Fragments arranged by size: 6417 683 56 BtsI GCAGTG_nn' Cuts at: 3419 3932 4672 5466 5486 3419 Size: 513 740 794 20 5089 Fragments arranged by size: 5089 794 740 513 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7156 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7121 DraI TTT'AAA Cuts at: 3096 3658 4460 3096 Size: 562 802 5792 Fragments arranged by size: 5792 802 562 DraIII CAC_nnn'GTG Cuts at: 3769 3769 Size: 7156 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6163 809 Size: 507 4847 1802 Fragments arranged by size: 4847 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2315 2414 Size: 54 1425 50 275 18 441 44 99 Cuts at: 2414 2490 2550 4435 4603 5492 7121 8 Size: 76 60 1885 168 889 1629 43 Fragments arranged by size: 1885 1629 1425 889 441 275 168 99 76 60 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2271 2490 2550 4603 1537 Size: 275 459 219 60 2053 4090 Fragments arranged by size: 4090 2053 459 275 219 60 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2534 2981 3582 5086 1353 Size: 731 450 447 601 1504 3423 Fragments arranged by size: 3423 1504 731 601 450 447 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2627 5743 6117 6263 387 Size: 1064 138 1038 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 1038 374 146 138 EcoICRI GAG'CTC Cuts at: 4187 4187 Size: 7156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2345 2873 2886 2907 3023 3446 Size: 393 437 276 528 13 21 116 423 Cuts at: 3446 3543 4041 4301 4361 5219 5816 6603 6871 Size: 97 498 260 60 858 597 787 268 Cuts at: 6871 1239 Size: 1524 Fragments arranged by size: 1524 858 787 597 528 498 437 423 393 276 268 260 116 97 60 21 13 EcoNI CCTnn'n_nnAGG Cuts at: 4171 4171 Size: 7156 EcoO109I rG'GnC_Cy Cuts at: 2074 2196 2207 2376 2429 2831 4429 2074 Size: 122 11 169 53 402 1598 4801 Fragments arranged by size: 4801 1598 402 169 122 53 11 EcoRI G'AATT_C Cuts at: 4465 4843 4465 Size: 378 6778 EcoRV GAT'ATC Cuts at: 2914 2914 Size: 7156 FseI GG_CCGG'CC Cuts at: 2262 2262 Size: 7156 FspI TGC'GCA Cuts at: 5662 5662 Size: 7156 HaeII r_GCGC'y Cuts at: 1678 2312 2332 2408 2447 2511 6303 1678 Size: 634 20 76 39 64 3792 2531 Fragments arranged by size: 3792 2531 634 76 64 39 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2980 3012 3118 3150 3914 Size: 32 135 32 1408 32 106 32 764 Cuts at: 3914 3946 5797 5829 5871 5903 1373 Size: 32 1851 32 42 32 2626 Fragments arranged by size: 2626 1851 1408 764 135 106 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4500 4746 669 Size: 988 281 2562 246 3079 Fragments arranged by size: 3079 2562 988 281 246 HindIII A'AGCT_T Cuts at: 748 2792 748 Size: 2044 5112 HpaI GTT'AAC Cuts at: 4746 4746 Size: 7156 KasI G'GCGC_C Cuts at: 2507 2507 Size: 7156 KpnI G_GTAC'C Cuts at: 4481 4481 Size: 7156 MfeI C'AATT_G Cuts at: 4755 4755 Size: 7156 MluI A'CGCG_T Cuts at: 7032 7032 Size: 7156 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 3246 3664 6270 6454 1305 Size: 1704 237 418 2606 184 2007 Fragments arranged by size: 2606 2007 1704 418 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4437 10 Size: 54 1425 343 2605 2729 Fragments arranged by size: 2729 2605 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5114 5473 5632 518 Size: 648 3948 359 159 2042 Fragments arranged by size: 3948 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2179 2253 2276 2286 2341 2467 3424 4572 Size: 573 74 23 10 55 126 957 1148 Cuts at: 4572 5249 6397 6642 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1148 957 677 573 245 126 74 55 23 10 NaeI GCC'GGC Cuts at: 2260 4212 2260 Size: 1952 5204 NarI GG'CG_CC Cuts at: 2508 2508 Size: 7156 NcoI C'CATG_G Cuts at: 513 1065 2022 2661 513 Size: 552 957 639 5008 Fragments arranged by size: 5008 957 639 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7156 NgoMIV G'CCGG_C Cuts at: 2258 4210 2258 Size: 1952 5204 NheI G'CTAG_C Cuts at: 1052 3049 1052 Size: 1997 5159 NotI GC'GGCC_GC Cuts at: 4603 4603 Size: 7156 NspI r_CATG'y Cuts at: 1132 1229 4514 6059 1132 Size: 97 3285 1545 2229 Fragments arranged by size: 3285 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7156 PciI A'CATG_T Cuts at: 6055 6055 Size: 7156 PflMI CCAn_nnn'nTGG Cuts at: 1108 2713 3424 1108 Size: 1605 711 4840 Fragments arranged by size: 4840 1605 711 PfoI T'CCnGG_A Cuts at: 2067 2577 4141 2067 Size: 510 1564 5082 Fragments arranged by size: 5082 1564 510 PmeI GTTT'AAAC Cuts at: 4460 4460 Size: 7156 PmlI CAC'GTG Cuts at: 4115 4115 Size: 7156 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2126 2158 5225 5257 6738 6770 2126 Size: 32 3067 32 1481 32 2512 Fragments arranged by size: 3067 2512 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2429 2831 4429 2429 Size: 402 1598 5156 Fragments arranged by size: 5156 1598 402 PshAI GACnn'nnGTC Cuts at: 1613 2829 1613 Size: 1216 5940 PsiI TTA'TAA Cuts at: 3287 4726 3287 Size: 1439 5717 PspOMI G'GGCC_C Cuts at: 2196 2207 2196 Size: 11 7145 PstI C_TGCA'G Cuts at: 830 2451 3375 4508 830 Size: 1621 924 1133 3478 Fragments arranged by size: 3478 1621 1133 924 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7156 PvuII CAG'CTG Cuts at: 2253 2467 3424 2253 Size: 214 957 5985 Fragments arranged by size: 5985 957 214 RsrII CG'GwC_CG Cuts at: 2065 2065 Size: 7156 SacI G_AGCT'C Cuts at: 4189 4189 Size: 7156 SacII CC_GC'GG Cuts at: 2277 2287 2342 2277 Size: 10 55 7091 Fragments arranged by size: 7091 55 10 SalI G'TCGA_C Cuts at: 1936 4498 1936 Size: 2562 4594 SanDI GG'GwC_CC Cuts at: 2429 2429 Size: 7156 SbfI CC_TGCA'GG Cuts at: 4508 4508 Size: 7156 ScaI AGT'ACT Cuts at: 2744 5404 7116 2744 Size: 2660 1712 2784 Fragments arranged by size: 2784 2660 1712 SfcI C'TryA_G Cuts at: 826 1045 2447 3371 4504 5639 6320 6511 826 Size: 219 1402 924 1133 1135 681 191 1471 Fragments arranged by size: 1471 1402 1135 1133 924 681 219 191 SfoI GGC'GCC Cuts at: 2509 2509 Size: 7156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7156 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7156 SmaI CCC'GGG Cuts at: 1872 4483 1872 Size: 2611 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3054 4020 4171 4352 5260 6161 Size: 197 926 1111 966 151 181 908 901 Cuts at: 6161 6423 820 Size: 262 1553 Fragments arranged by size: 1553 1111 966 926 908 901 262 197 181 151 SnaBI TAC'GTA Cuts at: 493 4453 4947 493 Size: 3960 494 2702 Fragments arranged by size: 3960 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7156 SphI G_CATG'C Cuts at: 1132 4514 1132 Size: 3382 3774 SspI AAT'ATT Cuts at: 5 52 5080 5 Size: 47 5028 2081 Fragments arranged by size: 5028 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7156 StyI C'CwwG_G Cuts at: 513 1065 2022 2661 2827 4122 4595 513 Size: 552 957 639 166 1295 473 3074 Fragments arranged by size: 3074 1295 957 639 552 473 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2047 5183 5200 5353 5538 5983 325 Size: 1255 467 3136 17 153 185 445 1498 Fragments arranged by size: 3136 1498 1255 467 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 5402 7114 Size: 274 80 33 51 1443 765 2660 1712 Cuts at: 7114 96 Size: 138 Fragments arranged by size: 2660 1712 1443 765 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3004 3036 7116 7148 3004 Size: 32 4080 32 3012 Fragments arranged by size: 4080 3012 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7156 XbaI T'CTAG_A Cuts at: 4492 4492 Size: 7156 XhoI C'TCGA_G Cuts at: 1943 3054 1943 Size: 1111 6045 XmaI C'CCGG_G Cuts at: 1870 4481 1870 Size: 2611 4545 XmnI GAAnn'nnTTC Cuts at: 3036 3135 5285 3036 Size: 99 2150 4907 Fragments arranged by size: 4907 2150 99 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4964 276 Size: 53 83 186 936 3430 2468 Fragments arranged by size: 3430 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BfrBI BsiWI BstZ17I FalI FspAI NruI NsiI PacI PspXI PsrI SapI SexAI SfiI SrfI SwaI XcmI