Restriction map for
pFN21ASDA1963
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BseRI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeIII MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 145 GGCGCC G[GA]^CG[CT]C
AflIII 308 ACACGT A^C[GA][CT]GT
AvaI 451 CCCGGG C^[CT]CG[GA]G
AvaII 185 GGACC G^G[AT]CC
BalI 302 TGGCCA TGG^CCA
BanII 577 GAGCTC G[GA]GC[CT]^C
BbeI 145 GGCGCC GGCGC^C
BbrPI 421 CACGTG CAC^GTG
BbsI 749 GTCTTC GAAGAC(2/6)
BfaI 922 CTAG C^TAG
BglI 137 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 749 GTCTTC GAAGAC(2/6)
BsaAI 421 CACGTG [CT]AC^GT[GA]
BsaHI 145 GGCGCC G[GA]^CG[CT]C
BsaI 609 GGTCTC GGTCTC(1/5)
BsiEI 208 CGGCCG CG[GA][CT]^CG
BsmFI 184 GGGAC GGGAC(10/14)
BsoBI 451 CCCGGG C^[CT]CG[GA]G
BspMI 416 ACCTGC ACCTGC(4/8)
BsrFI 694 ACCGGC [GA]^CCGG[CT]
BssSI 889 CTCGTG CACGAG(-5/-1)
BstAPI 733 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 809 GGATG GGATG(2/0)
BstXI 722 CCATCGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 694 ACCGGC [GA]^CCGG[CT]
DraII 184 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 208 CGGCCG C^GGCCG
EclXI 208 CGGCCG C^GGCCG
Eco52I 208 CGGCCG C^GGCCG
EcoNI 342 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 184 GGGACCC [GA]G^G[ACGT]CC[CT]
FokI 809 GGATG GGATG(9/13)
Hin1I 145 GGCGCC G[GA]^CG[CT]C
KasI 145 GGCGCC G^GCGCC
MaeI 922 CTAG C^TAG
MluNI 302 TGGCCA TGG^CCA
MscI 302 TGGCCA TGG^CCA
NarI 145 GGCGCC GG^CGCC
PleI 152 GACTC GAGTC(4/5)
PmaCI 421 CACGTG CAC^GTG
PmlI 421 CACGTG CAC^GTG
PpuMI 184 GGGACCC [GA]G^G[AT]CC[CT]
SacI 577 GAGCTC GAGCT^C
SfoI 145 GGCGCC GGC^GCC
SmaI 451 CCCGGG CCC^GGG
StuI 712 AGGCCT AGG^CCT
TfiI 817 GAATC G^A[AT]TC
XmaCI 451 CCCGGG C^CCGGG
XmaI 451 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 690 GTAC GT^AC
939 GTAC
AspHI 538 GTGCTC G[AT]GC[AT]^C
577 GAGCTC
BanI 145 GGCGCC G^G[CT][GA]CC
509 GGCACC
BpmI 574 CTGGAG CTGGAG(16/14)
699 CTGGAG
BsiHKAI 538 GTGCTC G[AT]GC[AT]^C
577 GAGCTC
BsmAI 204 GTCTC GTCTC(1/5)
610 GTCTC
BsrBI 97 CCGCTC CCGCTC(-3/-3)
157 CCGCTC
DdeI 470 CTGAG C^T[ACGT]AG
881 CTAAG
DsaI 138 CCGCGG C^C[GA][CT]GG
433 CCGCGG
EaeI 208 CGGCCG [CT]^GGCC[GA]
302 TGGCCA
EarI 541 CTCTTC CTCTTC(1/4)
580 CTCTTC
HgaI 352 GACGC GACGC(5/10)
789 GCGTC
HinfI 152 GACTC G^A[ACGT]TC
817 GAATC
Ksp632I 541 CTCTTC CTCTTC(1/4)
580 CTCTTC
KspI 138 CCGCGG CCGC^GG
433 CCGCGG
MseI 785 TTAA T^TAA
917 TTAA
MslI 57 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
723 CATCGACATG
NlaIII 63 CATG CATG^
729 CATG
PvuII 232 CAGCTG CAG^CTG
328 CAGCTG
RsaI 690 GTAC GT^AC
939 GTAC
SacII 138 CCGCGG CCGC^GG
433 CCGCGG
SapI 540 GCTCTTC GCTCTTC(1/4)
579 GCTCTTC
SfaNI 591 GATGC GCATC(5/9)
810 GATGC
SfcI 264 CTACAG C^T[GA][CT]AG
567 CTATAG
Tru9I 785 TTAA T^TAA
917 TTAA
Tsp509I 51 AATT ^AATT
960 AATT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AlwI BamHI
BbvI BcnI BmyI BsaJI BsgI
BsiYI BslI Bsp1286I BsrI BssHII
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TaqI TseI TspRI TthHB8I XhoII