(Circular) (Six-base) MAPSORT of: pFN21AA1962.seq Check: 2434 from: 1 to: 6937 pFN21AA1962 6937 bp With 182 enzymes: SgfI * March 28, 2012 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4747 278 Size: 53 83 186 936 3211 2468 Fragments arranged by size: 3211 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2973 4280 1937 Size: 1036 1307 4594 Fragments arranged by size: 4594 1307 1036 Acc65I G'GTAC_C Cuts at: 4258 4258 Size: 6937 AclI AA'CG_TT Cuts at: 1563 5064 5437 1563 Size: 3501 373 3063 Fragments arranged by size: 3501 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2931 3004 3102 3667 4003 5000 6384 6652 Size: 1299 73 98 565 336 997 1384 268 Cuts at: 6652 1632 Size: 1917 Fragments arranged by size: 1917 1384 1299 997 565 336 268 98 73 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6740 AflIII A'CryG_T Cuts at: 1203 3659 5836 6813 1203 Size: 2456 2177 977 1327 Fragments arranged by size: 2456 2177 1327 977 AgeI A'CCGG_T Cuts at: 5768 5768 Size: 6937 AhdI GACnn_n'nnGTC Cuts at: 5666 5666 Size: 6937 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6937 AlwNI CAG_nnn'CTG Cuts at: 2245 2305 4680 6252 2245 Size: 60 2375 1572 2930 Fragments arranged by size: 2930 2375 1572 60 ApaLI G'TGCA_C Cuts at: 4994 6150 4994 Size: 1156 5781 ApoI r'AATT_y Cuts at: 1493 2624 2842 2900 3381 3549 3633 3717 3775 Size: 1131 218 58 481 168 84 84 58 Cuts at: 3775 3801 3969 4053 4137 4246 4472 4624 6733 Size: 26 168 84 84 109 226 152 2109 Cuts at: 6733 1493 Size: 1697 Fragments arranged by size: 2109 1697 1131 481 226 218 168 168 152 109 84 84 84 84 58 58 26 AseI AT'TA_AT Cuts at: 160 1032 5491 160 Size: 872 4459 1606 Fragments arranged by size: 4459 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6937 AvaI C'yCGr_G Cuts at: 1870 1943 4262 1870 Size: 73 2319 4545 Fragments arranged by size: 4545 2319 73 BamHI G'GATC_C Cuts at: 4267 4634 4267 Size: 367 6570 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2757 4258 5713 618 Size: 325 217 627 970 1501 1455 1842 Fragments arranged by size: 1842 1501 1455 970 627 325 217 BanII G_rGCy'C Cuts at: 1391 2232 4256 1391 Size: 841 2024 4072 Fragments arranged by size: 4072 2024 841 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4187 928 Size: 971 2288 3678 Fragments arranged by size: 3678 2288 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5128 5162 1903 Size: 34 50 34 3107 34 3678 Fragments arranged by size: 3678 3107 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4829 6039 4829 Size: 1210 5727 BclI T'GATC_A Cuts at: 1548 2773 1548 Size: 1225 5712 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5548 136 Size: 107 122 71 631 605 3876 1525 Fragments arranged by size: 3876 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6932 6932 Size: 6937 BlpI GC'TnA_GC Cuts at: 4354 4354 Size: 6937 Bme1580I G_kGCm'C Cuts at: 1790 2022 4998 6154 1790 Size: 232 2976 1156 2573 Fragments arranged by size: 2976 2573 1156 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6937 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4668 5626 442 Size: 416 53 83 399 24 3251 958 1753 Fragments arranged by size: 3251 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6937 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2473 4050 2473 Size: 1577 5360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 3411 3579 3663 3747 3831 5597 1239 Size: 1346 826 168 84 84 84 1766 2579 Fragments arranged by size: 2579 1766 1346 826 168 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2412 2998 5062 5927 6225 2412 Size: 586 2064 865 298 3124 Fragments arranged by size: 3124 2064 865 586 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2242 3586 5600 882 Size: 617 743 1344 2014 2219 Fragments arranged by size: 2219 2014 1344 743 617 BsaAI yAC'GTr Cuts at: 493 1204 4728 493 Size: 711 3524 2702 Fragments arranged by size: 3524 2702 711 BsaBI GATnn'nnATC Cuts at: 2772 6931 2772 Size: 4159 2778 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4744 5126 5761 275 Size: 53 83 186 936 3211 382 635 1451 Fragments arranged by size: 3211 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4145 5370 5768 6042 6189 6600 4145 Size: 1225 398 274 147 411 4482 Fragments arranged by size: 4482 1225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2872 2902 1178 Size: 30 165 30 1469 30 5213 Fragments arranged by size: 5213 1469 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 2562 2834 1186 Size: 1279 97 272 5289 Fragments arranged by size: 5289 1279 272 97 BseYI C'CCAG_C Cuts at: 2664 2796 6140 6636 2664 Size: 132 3344 496 2965 Fragments arranged by size: 3344 2965 496 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6364 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4387 5148 6176 1540 Size: 275 186 2386 761 1028 2301 Fragments arranged by size: 2386 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 3529 4047 4998 5083 6154 2232 Size: 1297 518 951 85 1071 3015 Fragments arranged by size: 3015 1297 1071 951 518 85 BsmI GAATG_Cn' Cuts at: 4448 4541 4448 Size: 93 6844 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2232 3529 4047 4256 4998 5083 Size: 399 232 210 1297 518 209 742 85 Cuts at: 5083 6154 1391 Size: 1071 2174 Fragments arranged by size: 2174 1297 1071 742 518 399 232 210 209 85 BspEI T'CCGG_A Cuts at: 6600 6600 Size: 6937 BspHI T'CATG_A Cuts at: 4826 6556 4826 Size: 1730 5207 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4292 844 Size: 3448 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4824 1394 Size: 429 3001 3507 Fragments arranged by size: 3507 3001 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5432 5606 65 Size: 1183 4184 174 1396 Fragments arranged by size: 4184 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5581 5768 1700 Size: 110 3771 187 2869 Fragments arranged by size: 3771 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6937 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6937 BssSI C'ACGA_G Cuts at: 4997 6009 4997 Size: 1012 5925 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6937 BstBI TT'CG_AA Cuts at: 5741 5741 Size: 6937 BstEII G'GTnAC_C Cuts at: 1184 2374 1184 Size: 1190 5747 BstYI r'GATC_y Cuts at: 1972 2416 4267 4634 5019 5036 6488 6574 6586 Size: 444 1851 367 385 17 1452 86 12 Cuts at: 6586 6932 1972 Size: 346 1977 Fragments arranged by size: 1977 1851 1452 444 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2612 2762 2612 Size: 150 6787 BtgI C'CryG_G Cuts at: 513 1065 2687 513 Size: 552 1622 4763 Fragments arranged by size: 4763 1622 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3429 487 Size: 683 56 2203 3995 Fragments arranged by size: 3995 2203 683 56 BtsI GCAGTG_nn' Cuts at: 3947 4453 5247 5267 3947 Size: 506 794 20 5617 Fragments arranged by size: 5617 794 506 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6937 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6902 DraI TTT'AAA Cuts at: 4189 4241 4189 Size: 52 6885 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5944 809 Size: 507 4628 1802 Fragments arranged by size: 4628 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4384 5273 6902 Size: 54 1425 50 275 18 2554 889 1629 Cuts at: 6902 8 Size: 43 Fragments arranged by size: 2554 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4384 1537 Size: 275 2572 4090 Fragments arranged by size: 4090 2572 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2848 3121 4867 1353 Size: 1495 273 1746 3423 Fragments arranged by size: 3423 1746 1495 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5524 5898 6044 387 Size: 1064 138 3935 374 146 1280 Fragments arranged by size: 3935 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2230 2230 Size: 6937 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 2931 3004 3102 3411 3579 3663 Size: 393 953 346 73 98 309 168 84 Cuts at: 3663 3667 3747 3831 4003 5000 5597 6384 6652 Size: 4 80 84 172 997 597 787 268 Cuts at: 6652 1239 Size: 1524 Fragments arranged by size: 1524 997 953 787 597 393 346 309 268 172 168 98 84 84 80 73 4 EcoO109I rG'GnC_Cy Cuts at: 2086 3168 2086 Size: 1082 5855 EcoRI G'AATT_C Cuts at: 3381 3549 3633 3717 3775 3801 3969 4053 4246 Size: 168 84 84 58 26 168 84 193 Cuts at: 4246 4624 3381 Size: 378 5694 Fragments arranged by size: 5694 378 193 168 168 84 84 84 58 26 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3034 3034 Size: 6937 FspI TGC'GCA Cuts at: 5443 5443 Size: 6937 HaeII r_GCGC'y Cuts at: 1678 3360 6084 1678 Size: 1682 2724 2531 Fragments arranged by size: 2724 2531 1682 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2408 2440 3428 Size: 32 135 32 563 32 241 32 988 Cuts at: 3428 3460 3596 3628 3862 3894 5578 5610 5652 Size: 32 136 32 234 32 1684 32 42 Cuts at: 5652 5684 1373 Size: 32 2626 Fragments arranged by size: 2626 1684 988 563 241 234 136 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2219 4281 4527 669 Size: 988 281 281 2062 246 3079 Fragments arranged by size: 3079 2062 988 281 281 246 HindIII A'AGCT_T Cuts at: 748 3426 748 Size: 2678 4259 HpaI GTT'AAC Cuts at: 4527 4527 Size: 6937 KpnI G_GTAC'C Cuts at: 4262 4262 Size: 6937 MfeI C'AATT_G Cuts at: 3667 4536 3667 Size: 869 6068 MluI A'CGCG_T Cuts at: 6813 6813 Size: 6937 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3233 6051 6235 1305 Size: 1928 2818 184 2007 Fragments arranged by size: 2818 2007 1928 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5115 Fragments arranged by size: 5115 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3826 3978 4895 5254 5413 518 Size: 648 2660 152 917 359 159 2042 Fragments arranged by size: 2660 2042 917 648 359 159 152 MspA1I CmG'CkG Cuts at: 1606 4353 5030 6178 6423 1606 Size: 2747 677 1148 245 2120 Fragments arranged by size: 2747 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2687 513 Size: 552 1622 4763 Fragments arranged by size: 4763 1622 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6937 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6937 NotI GC'GGCC_GC Cuts at: 4384 4384 Size: 6937 NspI r_CATG'y Cuts at: 1132 1229 3663 4295 5840 1132 Size: 97 2434 632 1545 2229 Fragments arranged by size: 2434 2229 1545 632 97 PacI TTA_AT'TAA Cuts at: 2958 2958 Size: 6937 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6937 PciI A'CATG_T Cuts at: 3659 5836 3659 Size: 2177 4760 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6937 PmeI GTTT'AAAC Cuts at: 4241 4241 Size: 6937 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5006 5038 6519 6551 5006 Size: 32 1481 32 5392 Fragments arranged by size: 5392 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 3168 2086 Size: 1082 5855 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6937 PsiI TTA'TAA Cuts at: 3573 3993 4507 3573 Size: 420 514 6003 Fragments arranged by size: 6003 514 420 PstI C_TGCA'G Cuts at: 830 4234 4289 830 Size: 3404 55 3478 Fragments arranged by size: 3478 3404 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6937 SacI G_AGCT'C Cuts at: 2232 2232 Size: 6937 SalI G'TCGA_C Cuts at: 1936 4279 1936 Size: 2343 4594 SbfI CC_TGCA'GG Cuts at: 4289 4289 Size: 6937 ScaI AGT'ACT Cuts at: 5185 6897 5185 Size: 1712 5225 SfcI C'TryA_G Cuts at: 826 1045 2013 3352 4230 4285 5420 6101 6292 Size: 219 968 1339 878 55 1135 681 191 Cuts at: 6292 826 Size: 1471 Fragments arranged by size: 1471 1339 1135 968 878 681 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6937 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6937 SmaI CCC'GGG Cuts at: 1872 4264 1872 Size: 2392 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2427 2977 5041 5942 6204 820 Size: 197 926 484 550 2064 901 262 1553 Fragments arranged by size: 2064 1553 926 901 550 484 262 197 SnaBI TAC'GTA Cuts at: 493 4728 493 Size: 4235 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6937 SphI G_CATG'C Cuts at: 1132 4295 1132 Size: 3163 3774 SspI AAT'ATT Cuts at: 5 52 2804 4861 5 Size: 47 2752 2057 2081 Fragments arranged by size: 2752 2081 2057 47 StuI AGG'CCT Cuts at: 1517 4203 1517 Size: 2686 4251 StyI C'CwwG_G Cuts at: 513 1065 2687 4376 513 Size: 552 1622 1689 3074 Fragments arranged by size: 3074 1689 1622 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4964 4981 5134 5319 5764 325 Size: 1255 3384 17 153 185 445 1498 Fragments arranged by size: 3384 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5183 6895 96 Size: 274 80 33 51 1443 3206 1712 138 Fragments arranged by size: 3206 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2470 2502 6897 6929 2470 Size: 32 4395 32 2478 Fragments arranged by size: 4395 2478 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6937 XbaI T'CTAG_A Cuts at: 4273 4273 Size: 6937 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2804 2804 Size: 6937 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6937 XmaI C'CCGG_G Cuts at: 1870 4262 1870 Size: 2392 4545 XmnI GAAnn'nnTTC Cuts at: 3017 3485 3512 3932 4016 5066 3017 Size: 468 27 420 84 1050 4888 Fragments arranged by size: 4888 1050 468 420 84 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4745 276 Size: 53 83 186 936 3211 2468 Fragments arranged by size: 3211 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI