(Circular) (Six-base) MAPSORT of: pFN21ASDA1957.seq Check: 6534 from: 1 to: 5992 pFN21ASDA1957 5992 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3006 3006 Size: 5992 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2462 3802 278 Size: 53 83 186 936 926 1340 2468 Fragments arranged by size: 2468 1340 936 926 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2354 2638 4055 5439 5707 1632 Size: 722 284 1417 1384 268 1917 Fragments arranged by size: 1917 1417 1384 722 284 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 2037 4891 5868 1203 Size: 834 2854 977 1327 Fragments arranged by size: 2854 1327 977 834 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 2383 2677 4721 2383 Size: 294 2044 3654 Fragments arranged by size: 3654 2044 294 AleI CACnn'nnGTG Cuts at: 1166 3107 1166 Size: 1941 4051 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2345 1184 Size: 1161 4831 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2501 2533 2501 Size: 32 5960 AlwNI CAG_nnn'CTG Cuts at: 2254 3735 5307 2254 Size: 1481 1572 2939 Fragments arranged by size: 2939 1572 1481 ApaI G_GGCC'C Cuts at: 2130 2201 2234 2612 2632 2936 3168 2130 Size: 71 33 378 20 304 232 4954 Fragments arranged by size: 4954 378 304 232 71 33 20 ApaLI G'TGCA_C Cuts at: 2993 4049 5205 2993 Size: 1056 1156 3780 Fragments arranged by size: 3780 1156 1056 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 2085 2194 2843 2891 2937 3242 3317 Size: 73 142 109 649 48 46 305 75 Cuts at: 3317 1870 Size: 4545 Fragments arranged by size: 4545 649 305 142 109 75 73 48 46 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2423 2443 2568 2853 2872 Size: 325 217 627 636 20 125 285 19 Cuts at: 2872 3073 3313 4768 618 Size: 201 240 1455 1842 Fragments arranged by size: 1842 1455 636 627 325 285 240 217 201 125 20 19 BanII G_rGCy'C Cuts at: 1391 2130 2201 2234 2291 2492 2539 2555 2612 Size: 739 71 33 57 201 47 16 57 Cuts at: 2612 2632 2642 2936 3168 3311 1391 Size: 20 10 294 232 143 4072 Fragments arranged by size: 4072 739 294 232 201 143 71 57 57 47 33 20 16 10 BbeI G_GCGC'C Cuts at: 2572 2857 2572 Size: 285 5707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3124 928 Size: 971 1225 3796 Fragments arranged by size: 3796 1225 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3088 3122 4183 4217 1903 Size: 34 1151 34 1061 34 3678 Fragments arranged by size: 3678 1151 1061 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2264 2309 2480 Size: 107 122 71 631 605 592 45 171 Cuts at: 2480 2802 2940 2978 4603 136 Size: 322 138 38 1625 1525 Fragments arranged by size: 1625 1525 631 605 592 322 171 138 122 107 71 45 38 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 2130 2201 2234 2448 2612 2632 2875 2936 Size: 340 71 33 214 164 20 243 61 Cuts at: 2936 2997 3168 4053 5209 1790 Size: 61 171 885 1156 2573 Fragments arranged by size: 2573 1156 885 340 243 214 171 164 71 61 61 33 20 BmgBI CAC'GTC Cuts at: 1318 2241 1318 Size: 923 5069 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3723 4681 442 Size: 416 53 83 399 24 2306 958 1753 Fragments arranged by size: 2306 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4652 1239 Size: 3413 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3209 4117 4982 5280 3209 Size: 908 865 298 3921 Fragments arranged by size: 3921 908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4655 882 Size: 617 3156 2219 Fragments arranged by size: 3156 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2353 2929 3289 3783 493 Size: 711 1149 576 360 494 2702 Fragments arranged by size: 2702 1149 711 576 494 360 BsaBI GATnn'nnATC Cuts at: 2360 5986 2360 Size: 3626 2366 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2150 2403 2459 2569 Size: 53 83 186 936 617 253 56 110 Cuts at: 2569 2835 2854 3036 3799 4181 4816 275 Size: 266 19 182 763 382 635 1451 Fragments arranged by size: 1451 936 763 635 617 382 266 253 186 182 110 83 56 53 19 BsaWI w'CCGG_w Cuts at: 3177 4425 4823 5097 5244 5655 3177 Size: 1248 398 274 147 411 3514 Fragments arranged by size: 3514 1248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 2501 2531 1178 Size: 30 165 30 699 30 369 30 4639 Fragments arranged by size: 4639 699 369 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 1186 Size: 1219 4773 BseYI C'CCAG_C Cuts at: 2227 5195 5691 2227 Size: 2968 496 2528 Fragments arranged by size: 2968 2528 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5419 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2647 2688 3025 3442 4203 5231 Size: 275 186 646 41 337 417 761 1028 Cuts at: 5231 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 646 417 337 275 186 41 BsiHKAI G_wGCw'C Cuts at: 2532 2919 2997 4053 4138 5209 2532 Size: 387 78 1056 85 1071 3315 Fragments arranged by size: 3315 1071 1056 387 85 78 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2201 2234 2291 2448 2492 2532 Size: 399 340 71 33 57 157 44 40 Cuts at: 2532 2539 2555 2612 2632 2642 2875 2919 2936 Size: 7 16 57 20 10 233 44 17 Cuts at: 2936 2997 3168 3311 4053 4138 5209 1391 Size: 61 171 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 340 233 171 157 143 85 71 61 57 57 44 44 40 33 20 17 16 10 7 BspEI T'CCGG_A Cuts at: 3177 5655 3177 Size: 2478 3514 BspHI T'CATG_A Cuts at: 2634 3881 5611 2634 Size: 1247 1730 3015 Fragments arranged by size: 3015 1730 1247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3006 3347 844 Size: 2162 341 3489 Fragments arranged by size: 3489 2162 341 BsrBI CCG'CTC Cuts at: 1394 1823 2053 2724 3879 1394 Size: 429 230 671 1155 3507 Fragments arranged by size: 3507 1155 671 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 2146 4487 4661 65 Size: 1183 898 2341 174 1396 Fragments arranged by size: 2341 1396 1183 898 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2850 2972 4636 4823 1700 Size: 110 1040 122 1664 187 2869 Fragments arranged by size: 2869 1664 1040 187 122 110 BsrGI T'GTAC_A Cuts at: 96 2133 96 Size: 2037 3955 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 2497 4052 5064 2497 Size: 1555 1012 3425 Fragments arranged by size: 3425 1555 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3051 794 Size: 2257 3735 BstBI TT'CG_AA Cuts at: 2007 4796 2007 Size: 2789 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2909 2909 Size: 5992 BstYI r'GATC_y Cuts at: 1972 2160 3322 3689 4074 4091 5543 5629 5641 Size: 188 1162 367 385 17 1452 86 12 Cuts at: 5641 5987 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1162 385 367 346 188 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3191 3191 Size: 5992 BtgI C'CryG_G Cuts at: 513 1065 2202 2282 2790 2800 513 Size: 552 1137 80 508 10 3705 Fragments arranged by size: 3705 1137 552 508 80 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 3508 4302 4322 3508 Size: 794 20 5178 Fragments arranged by size: 5178 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DraIII CAC_nnn'GTG Cuts at: 2791 2791 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2481 2644 2685 Size: 54 1425 50 275 18 651 163 41 Cuts at: 2685 2958 2974 3003 3439 4328 5957 8 Size: 273 16 29 436 889 1629 43 Fragments arranged by size: 1629 1425 889 651 436 275 273 163 54 50 43 41 29 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2644 2685 3439 1537 Size: 275 832 41 754 4090 Fragments arranged by size: 4090 832 754 275 41 EarI CTCTTCn'nnn_ Cuts at: 1353 3922 1353 Size: 2569 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2638 4055 4652 5439 5707 1239 Size: 393 722 284 1417 597 787 268 1524 Fragments arranged by size: 1524 1417 787 722 597 393 284 268 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2333 Size: 5992 EcoO109I rG'GnC_Cy Cuts at: 2126 2127 2198 2231 2608 2609 2628 2629 2679 Size: 1 71 33 377 1 19 1 50 Cuts at: 2679 2706 2933 3013 3164 2126 Size: 27 227 80 151 4954 Fragments arranged by size: 4954 377 227 151 80 71 50 33 27 19 1 1 1 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FseI GG_CCGG'CC Cuts at: 2976 2976 Size: 5992 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 2572 2857 5139 1678 Size: 894 285 2282 2531 Fragments arranged by size: 2531 2282 894 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2369 2401 2663 Size: 32 135 32 530 32 235 32 262 Cuts at: 2663 2695 4633 4665 4707 4739 1373 Size: 32 1938 32 42 32 2626 Fragments arranged by size: 2626 1938 530 262 235 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2148 3336 3582 669 Size: 988 281 210 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 210 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KasI G'GCGC_C Cuts at: 2568 2853 2568 Size: 285 5707 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 5106 5290 1305 Size: 918 2883 184 2007 Fragments arranged by size: 2883 2007 918 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4170 Fragments arranged by size: 4170 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3107 3950 4309 4468 518 Size: 648 1941 843 359 159 2042 Fragments arranged by size: 2042 1941 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 2582 3408 4085 5233 5478 1606 Size: 598 378 826 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 677 598 378 245 NaeI GCC'GGC Cuts at: 2852 2974 2852 Size: 122 5870 NarI GG'CG_CC Cuts at: 2569 2854 2569 Size: 285 5707 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5440 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NgoMIV G'CCGG_C Cuts at: 2850 2972 2850 Size: 122 5870 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2041 3350 4895 1132 Size: 97 812 1309 1545 2229 Fragments arranged by size: 2229 1545 1309 812 97 PasI CC'CwG_GG Cuts at: 1348 2419 2558 2613 1348 Size: 1071 139 55 4727 Fragments arranged by size: 4727 1071 139 55 PciI A'CATG_T Cuts at: 2037 4891 2037 Size: 2854 3138 PflMI CCAn_nnn'nTGG Cuts at: 1108 2040 1108 Size: 932 5060 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PmlI CAC'GTG Cuts at: 2353 2929 2353 Size: 576 5416 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2243 2275 2501 2533 4061 4093 5574 5606 2243 Size: 32 226 32 1528 32 1481 32 2629 Fragments arranged by size: 2629 1528 1481 226 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2679 2706 2679 Size: 27 5965 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 2126 2197 2230 2608 2628 2932 3164 2126 Size: 71 33 378 20 304 232 4954 Fragments arranged by size: 4954 378 304 232 71 33 20 PstI C_TGCA'G Cuts at: 830 3090 3344 830 Size: 2260 254 3478 Fragments arranged by size: 3478 2260 254 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2582 2582 Size: 5992 SacII CC_GC'GG Cuts at: 2205 2205 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SanDI GG'GwC_CC Cuts at: 2706 2706 Size: 5992 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SfcI C'TryA_G Cuts at: 826 1045 3086 3340 4475 5156 5347 826 Size: 219 2041 254 1135 681 191 1471 Fragments arranged by size: 2041 1471 1135 681 254 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2264 2978 2264 Size: 714 5278 SfoI GGC'GCC Cuts at: 2570 2855 2570 Size: 285 5707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 2087 2845 3319 1872 Size: 215 758 474 4545 Fragments arranged by size: 4545 758 474 215 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 4096 4997 5259 820 Size: 197 926 1281 872 901 262 1553 Fragments arranged by size: 1553 1281 926 901 872 262 197 SnaBI TAC'GTA Cuts at: 493 3289 3783 493 Size: 2796 494 2702 Fragments arranged by size: 2796 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 2117 1517 Size: 600 5392 StyI C'CwwG_G Cuts at: 513 1065 2121 2448 2586 2766 3431 513 Size: 552 1056 327 138 180 665 3074 Fragments arranged by size: 3074 1056 665 552 327 180 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4019 4036 4189 4374 4819 325 Size: 1255 2439 17 153 185 445 1498 Fragments arranged by size: 2439 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 4238 5950 Size: 274 80 33 51 1443 156 2105 1712 Cuts at: 5950 96 Size: 138 Fragments arranged by size: 2105 1712 1443 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5952 5984 5952 Size: 32 5960 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 2085 2843 3317 1870 Size: 215 758 474 4545 Fragments arranged by size: 4545 758 474 215 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2460 3800 276 Size: 53 83 186 936 926 1340 2468 Fragments arranged by size: 2468 1340 936 926 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SexAI SrfI SwaI XcmI