(Circular) (Six-base) MAPSORT of: pFN21ASDA1956.seq Check: 4675 from: 1 to: 6757 pFN21ASDA1956 6757 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2234 2234 Size: 6757 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4567 278 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4100 1937 Size: 2163 4594 Acc65I G'GTAC_C Cuts at: 4078 4078 Size: 6757 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3901 4820 6204 6472 1632 Size: 2269 919 1384 268 1917 Fragments arranged by size: 2269 1917 1384 919 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6560 AflIII A'CryG_T Cuts at: 1203 2668 5656 6633 1203 Size: 1465 2988 977 1327 Fragments arranged by size: 2988 1465 1327 977 AgeI A'CCGG_T Cuts at: 5588 5588 Size: 6757 AhdI GACnn_n'nnGTC Cuts at: 5486 5486 Size: 6757 AleI CACnn'nnGTG Cuts at: 1166 2271 2355 3117 1166 Size: 1105 84 762 4806 Fragments arranged by size: 4806 1105 762 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6757 AlwNI CAG_nnn'CTG Cuts at: 2347 4500 6072 2347 Size: 2153 1572 3032 Fragments arranged by size: 3032 2153 1572 ApaLI G'TGCA_C Cuts at: 4814 5970 4814 Size: 1156 5601 ApoI r'AATT_y Cuts at: 1493 3740 4066 4292 4444 6553 1493 Size: 2247 326 226 152 2109 1697 Fragments arranged by size: 2247 2109 1697 326 226 152 AseI AT'TA_AT Cuts at: 160 1032 5311 160 Size: 872 4279 1606 Fragments arranged by size: 4279 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 3864 4082 1870 Size: 73 1921 218 4545 Fragments arranged by size: 4545 1921 218 73 BamHI G'GATC_C Cuts at: 4087 4454 4087 Size: 367 6390 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 3170 4078 5533 618 Size: 325 217 627 398 985 908 1455 1842 Fragments arranged by size: 1842 1455 985 908 627 398 325 217 BanII G_rGCy'C Cuts at: 1391 3674 4076 1391 Size: 2283 402 4072 Fragments arranged by size: 4072 2283 402 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5786 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4948 4982 1903 Size: 34 3011 34 3678 Fragments arranged by size: 3678 3011 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4649 5859 4649 Size: 1210 5547 BclI T'GATC_A Cuts at: 1548 1548 Size: 6757 BfrBI ATG'CAT Cuts at: 2646 2646 Size: 6757 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5368 136 Size: 107 122 71 631 605 3696 1525 Fragments arranged by size: 3696 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6752 6752 Size: 6757 BlpI GC'TnA_GC Cuts at: 4174 4174 Size: 6757 Bme1580I G_kGCm'C Cuts at: 1790 2033 3173 4818 5974 1790 Size: 243 1140 1645 1156 2573 Fragments arranged by size: 2573 1645 1156 1140 243 BmgBI CAC'GTC Cuts at: 1318 2119 1318 Size: 801 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 2577 2790 Size: 416 53 83 399 24 726 434 213 Cuts at: 2790 2874 4488 5446 442 Size: 84 1614 958 1753 Fragments arranged by size: 1753 1614 958 726 434 416 399 213 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2970 3054 3306 3390 3474 3558 3642 Size: 908 823 84 252 84 84 84 84 Cuts at: 3642 3733 3801 3969 5417 1239 Size: 91 68 168 1448 2579 Fragments arranged by size: 2579 1448 908 823 252 168 91 84 84 84 84 84 68 Bpu10I CC'TnA_GC Cuts at: 2536 2536 Size: 6757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4882 5747 6045 4882 Size: 865 298 5594 Fragments arranged by size: 5594 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5420 882 Size: 617 3921 2219 Fragments arranged by size: 3921 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2671 4054 4548 493 Size: 711 1467 1383 494 2702 Fragments arranged by size: 2702 1467 1383 711 494 BsaBI GATnn'nnATC Cuts at: 6751 6751 Size: 6757 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4564 4946 5581 275 Size: 53 83 186 936 3031 382 635 1451 Fragments arranged by size: 3031 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5190 5588 5862 6009 6420 5190 Size: 398 274 147 411 5527 Fragments arranged by size: 5527 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6532 Fragments arranged by size: 6532 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2195 2504 1186 Size: 901 108 309 5439 Fragments arranged by size: 5439 901 309 108 BseYI C'CCAG_C Cuts at: 2531 2718 5960 6456 2531 Size: 187 3242 496 2832 Fragments arranged by size: 3242 2832 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 3745 3904 3988 1163 Size: 573 597 1412 159 84 3932 Fragments arranged by size: 3932 1412 597 573 159 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4207 4968 5996 1540 Size: 275 186 2206 761 1028 2301 Fragments arranged by size: 2301 2206 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3674 4818 4903 5974 3674 Size: 1144 85 1071 4457 Fragments arranged by size: 4457 1144 1071 85 BsmI GAATG_Cn' Cuts at: 2648 3803 4268 4361 2648 Size: 1155 465 93 5044 Fragments arranged by size: 5044 1155 465 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 3173 3674 4076 4818 4903 5974 Size: 399 243 1140 501 402 742 85 1071 Cuts at: 5974 1391 Size: 2174 Fragments arranged by size: 2174 1140 1071 742 501 402 399 243 85 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 4646 6376 4646 Size: 1730 5027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2234 4112 844 Size: 1390 1878 3489 Fragments arranged by size: 3489 1878 1390 BsrBI CCG'CTC Cuts at: 1394 1823 4644 1394 Size: 429 2821 3507 Fragments arranged by size: 3507 2821 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5252 5426 65 Size: 1183 4004 174 1396 Fragments arranged by size: 4004 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5401 5588 1700 Size: 110 3591 187 2869 Fragments arranged by size: 3591 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6757 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6757 BssSI C'ACGA_G Cuts at: 4817 5829 4817 Size: 1012 5745 BstAPI GCAn_nnn'nTGC Cuts at: 794 2347 794 Size: 1553 5204 BstBI TT'CG_AA Cuts at: 2007 5561 2007 Size: 3554 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6757 BstYI r'GATC_y Cuts at: 1972 4087 4454 4839 4856 6308 6394 6406 6752 Size: 2115 367 385 17 1452 86 12 346 Cuts at: 6752 1972 Size: 1977 Fragments arranged by size: 2115 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3260 3260 Size: 6757 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6205 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6018 Fragments arranged by size: 6018 683 56 BtsI GCAGTG_nn' Cuts at: 2446 3702 3976 4273 5067 5087 2446 Size: 1256 274 297 794 20 4116 Fragments arranged by size: 4116 1256 794 297 274 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6757 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6722 DraI TTT'AAA Cuts at: 3844 4061 3844 Size: 217 6540 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5764 809 Size: 507 4448 1802 Fragments arranged by size: 4448 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4204 5093 6722 Size: 54 1425 50 275 18 2374 889 1629 Cuts at: 6722 8 Size: 43 Fragments arranged by size: 2374 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4204 1537 Size: 275 2392 4090 Fragments arranged by size: 4090 2392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 2459 3679 4006 4687 1353 Size: 839 267 1220 327 681 3423 Fragments arranged by size: 3423 1220 839 681 327 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5344 5718 5864 387 Size: 1064 138 3755 374 146 1280 Fragments arranged by size: 3755 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3672 3672 Size: 6757 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2970 3054 3306 3390 3474 3558 Size: 393 515 823 84 252 84 84 84 Cuts at: 3558 3642 3733 3801 3901 3969 4820 5417 6204 Size: 84 91 68 100 68 851 597 787 Cuts at: 6204 6472 1239 Size: 268 1524 Fragments arranged by size: 1524 851 823 787 597 515 393 268 252 100 91 84 84 84 84 84 68 68 EcoNI CCTnn'n_nnAGG Cuts at: 2090 2241 2090 Size: 151 6606 EcoRI G'AATT_C Cuts at: 4066 4444 4066 Size: 378 6379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4059 4059 Size: 6757 FspI TGC'GCA Cuts at: 3837 5263 3837 Size: 1426 5331 HaeII r_GCGC'y Cuts at: 1678 5904 1678 Size: 4226 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2480 2512 2585 2617 5398 Size: 32 135 32 908 32 73 32 2781 Cuts at: 5398 5430 5472 5504 1373 Size: 32 42 32 2626 Fragments arranged by size: 2781 2626 908 135 73 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4101 4347 669 Size: 988 281 2163 246 3079 Fragments arranged by size: 3079 2163 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6757 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KpnI G_GTAC'C Cuts at: 4082 4082 Size: 6757 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5871 6055 1305 Size: 4566 184 2007 Fragments arranged by size: 4566 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4935 Fragments arranged by size: 4935 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2271 2355 3117 3469 4715 5074 5233 Size: 648 1105 84 762 352 1246 359 159 Cuts at: 5233 518 Size: 2042 Fragments arranged by size: 2042 1246 1105 762 648 359 352 159 84 MspA1I CmG'CkG Cuts at: 1606 2634 4173 4850 5998 6243 1606 Size: 1028 1539 677 1148 245 2120 Fragments arranged by size: 2120 1539 1148 1028 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6205 NdeI CA'TA_TG Cuts at: 387 3466 387 Size: 3079 3678 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6757 NotI GC'GGCC_GC Cuts at: 4204 4204 Size: 6757 NsiI A_TGCA'T Cuts at: 2648 2648 Size: 6757 NspI r_CATG'y Cuts at: 1132 1229 4115 5660 1132 Size: 97 2886 1545 2229 Fragments arranged by size: 2886 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2152 1348 Size: 804 5953 PciI A'CATG_T Cuts at: 5656 5656 Size: 6757 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6757 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4826 4858 6339 6371 4826 Size: 32 1481 32 5212 Fragments arranged by size: 5212 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6757 PsiI TTA'TAA Cuts at: 4327 4327 Size: 6757 PspXI vC'TCGA_Gb Cuts at: 3864 3864 Size: 6757 PstI C_TGCA'G Cuts at: 830 2553 3709 4109 830 Size: 1723 1156 400 3478 Fragments arranged by size: 3478 1723 1156 400 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6757 PvuII CAG'CTG Cuts at: 2634 2634 Size: 6757 SacI G_AGCT'C Cuts at: 3674 3674 Size: 6757 SalI G'TCGA_C Cuts at: 1936 4099 1936 Size: 2163 4594 SapI GCTCTTCn'nnn_ Cuts at: 2192 3679 4006 2192 Size: 1487 327 4943 Fragments arranged by size: 4943 1487 327 SbfI CC_TGCA'GG Cuts at: 4109 4109 Size: 6757 ScaI AGT'ACT Cuts at: 5005 6717 5005 Size: 1712 5045 SfcI C'TryA_G Cuts at: 826 1045 2431 2549 3705 4105 5240 5921 6112 Size: 219 1386 118 1156 400 1135 681 191 Cuts at: 6112 826 Size: 1471 Fragments arranged by size: 1471 1386 1156 1135 681 400 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6757 SmaI CCC'GGG Cuts at: 1872 4084 1872 Size: 2212 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3864 4861 5762 6024 820 Size: 197 926 1921 997 901 262 1553 Fragments arranged by size: 1921 1553 997 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4054 4548 493 Size: 3561 494 2702 Fragments arranged by size: 3561 2702 494 SpeI A'CTAG_T Cuts at: 152 3116 152 Size: 2964 3793 SphI G_CATG'C Cuts at: 1132 4115 1132 Size: 2983 3774 SspI AAT'ATT Cuts at: 5 52 4681 5 Size: 47 4629 2081 Fragments arranged by size: 4629 2081 47 StuI AGG'CCT Cuts at: 1517 3379 3631 3790 3958 1517 Size: 1862 252 159 168 4316 Fragments arranged by size: 4316 1862 252 168 159 StyI C'CwwG_G Cuts at: 513 1065 2416 4196 513 Size: 552 1351 1780 3074 Fragments arranged by size: 3074 1780 1351 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4784 4801 4954 5139 5584 325 Size: 1255 3204 17 153 185 445 1498 Fragments arranged by size: 3204 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3940 5003 6715 Size: 274 80 33 51 1443 1963 1063 1712 Cuts at: 6715 96 Size: 138 Fragments arranged by size: 1963 1712 1443 1063 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6717 6749 6717 Size: 32 6725 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6757 XbaI T'CTAG_A Cuts at: 2699 4093 2699 Size: 1394 5363 XhoI C'TCGA_G Cuts at: 1943 3864 1943 Size: 1921 4836 XmaI C'CCGG_G Cuts at: 1870 4082 1870 Size: 2212 4545 XmnI GAAnn'nnTTC Cuts at: 3743 4000 4886 3743 Size: 257 886 5614 Fragments arranged by size: 5614 886 257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4565 276 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI