Restriction map for
pFN21ASDA1951
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BciVI BclI BfrI BglI BglII
BlnI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1087 GTAGAC GT^[AC][GT]AC
AcyI 1409 GGCGTC G[GA]^CG[CT]C
AocI 625 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1604 AGCGCT AGC^GCT
AvaII 1779 GGTCC G^G[AT]CC
AviII 1460 TGCGCA TGC^GCA
BalI 1535 TGGCCA TGG^CCA
BbrPI 1456 CACGTG CAC^GTG
BbsI 1018 GTCTTC GAAGAC(2/6)
BcgI 1161 GCATCGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 667 CTAG C^TAG
BlpI 505 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 505 GCTGAGC GC^T[ACGT]AGC
BpuAI 1018 GTCTTC GAAGAC(2/6)
BsaAI 1456 CACGTG [CT]AC^GT[GA]
BsaBI 1820 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1409 GGCGTC G[GA]^CG[CT]C
BsmI 334 GAATGC GAATGC(1/-1)
Bsp1407I 1000 TGTACA T^GTACA
BsrGI 1000 TGTACA T^GTACA
BstYI 410 AGATCC [GA]^GATC[CT]
Bsu36I 625 CCTGAGG CC^T[ACGT]AGG
CelII 505 GCTGAGC GC^T[ACGT]AGC
Eco47III 1604 AGCGCT AGC^GCT
Eco81I 625 CCTGAGG CC^T[ACGT]AGG
EcoNI 1246 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1460 TGCGCA TGC^GCA
Hin1I 1409 GGCGTC G[GA]^CG[CT]C
KspI 1098 CCGCGG CCGC^GG
MaeI 667 CTAG C^TAG
MamI 1820 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 410 AGATCC [GA]^GATC[CT]
MluNI 1535 TGGCCA TGG^CCA
MscI 1535 TGGCCA TGG^CCA
NspI 236 ACATGC [GA]CATG^[CT]
PmaCI 1456 CACGTG CAC^GTG
PmlI 1456 CACGTG CAC^GTG
SacII 1098 CCGCGG CCGC^GG
SspBI 1000 TGTACA T^GTACA
TaqI 474 TCGA T^CGA
TthHB8I 474 TCGA T^CGA
XhoII 410 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 1001 GTAC GT^AC
1390 GTAC
Alw44I 1571 GTGCAC G^TGCAC
1872 GTGCAC
AlwI 411 GATCC GGATC(4/5)
1452 GATCC
AlwNI 436 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1915 CAGCCACTG
ApaLI 1571 GTGCAC G^TGCAC
1872 GTGCAC
AvaI 454 CCCGAG C^[CT]CG[GA]G
1787 CCCGAG
BsaI 1888 GAGACC GGTCTC(1/5)
1696 GGTCTC
BsgI 1955 CTGCAC GTGCAG(16/14)
1385 GTGCAG
BsoBI 454 CCCGAG C^[CT]CG[GA]G
1787 CCCGAG
BsrDI 1157 CATTGC GCAATG(2/0)
683 GCAATG
BstAPI 231 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1044 GCAGCACTTGC
BstXI 978 CCATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1506 CCACTCATGTGG
HaeII 1604 AGCGCT [GA]GCGC^[CT]
1923 GGCGCT
HgaI 1803 GACGC GACGC(5/10)
1410 GCGTC
HincII 66 GTCAAC GT[CT]^[GA]AC
99 GTCAAC
HindII 66 GTCAAC GT[CT]^[GA]AC
99 GTCAAC
NaeI 863 GCCGGC GCC^GGC
1228 GCCGGC
NcoI 758 CCATGG C^CATGG
1751 CCATGG
NgoMIV 863 GCCGGC G^CCGGC
1228 GCCGGC
PflMI 1584 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1914 CCAGCCACTGG
RsaI 1001 GTAC GT^AC
1390 GTAC
SmlI 1039 CTTGAG C^T[CT][GA]AG
1927 CTTGAG
Van91I 1584 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1914 CCAGCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
BanI BanII BbvCI BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SgrAI StyI TseI Tsp45I
TspRI