(Circular) (Six-base) MAPSORT of: pFN21ASDA1917.seq Check: 2612 from: 1 to: 6766 pFN21ASDA1917 6766 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3249 3249 Size: 6766 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4576 278 Size: 53 83 186 936 3040 2468 Fragments arranged by size: 3040 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2745 4059 4109 1937 Size: 808 1314 50 4594 Fragments arranged by size: 4594 1314 808 50 Acc65I G'GTAC_C Cuts at: 4087 4087 Size: 6766 AclI AA'CG_TT Cuts at: 1563 4893 5266 1563 Size: 3330 373 3063 Fragments arranged by size: 3330 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 2750 2841 2975 3096 3675 4829 6213 Size: 558 560 91 134 121 579 1154 1384 Cuts at: 6213 6481 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1154 579 560 558 268 134 121 91 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6569 AflIII A'CryG_T Cuts at: 1203 5665 6642 1203 Size: 4462 977 1327 Fragments arranged by size: 4462 1327 977 AgeI A'CCGG_T Cuts at: 5597 5597 Size: 6766 AhdI GACnn_n'nnGTC Cuts at: 2074 3277 3499 5495 2074 Size: 1203 222 1996 3345 Fragments arranged by size: 3345 1996 1203 222 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6766 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6766 AlwNI CAG_nnn'CTG Cuts at: 4509 6081 4509 Size: 1572 5194 ApaI G_GGCC'C Cuts at: 2032 3023 3150 3703 4049 2032 Size: 991 127 553 346 4749 Fragments arranged by size: 4749 991 553 346 127 ApaLI G'TGCA_C Cuts at: 4823 5979 4823 Size: 1156 5610 ApoI r'AATT_y Cuts at: 1493 4075 4301 4453 6562 1493 Size: 2582 226 152 2109 1697 Fragments arranged by size: 2582 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5320 160 Size: 872 4288 1606 Fragments arranged by size: 4288 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 AvaI C'yCGr_G Cuts at: 1870 1943 2406 4091 1870 Size: 73 463 1685 4545 Fragments arranged by size: 4545 1685 463 73 BamHI G'GATC_C Cuts at: 2557 4096 4463 2557 Size: 1539 367 4860 Fragments arranged by size: 4860 1539 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3944 4087 5542 618 Size: 325 217 627 2157 143 1455 1842 Fragments arranged by size: 2157 1842 1455 627 325 217 143 BanII G_rGCy'C Cuts at: 1391 2032 2342 3023 3150 3703 4049 4085 1391 Size: 641 310 681 127 553 346 36 4072 Fragments arranged by size: 4072 681 641 553 346 310 127 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2273 2829 928 Size: 971 374 556 4865 Fragments arranged by size: 4865 971 556 374 BbvCI CC'TCA_GC Cuts at: 2325 2325 Size: 6766 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4957 4991 1903 Size: 34 3020 34 3678 Fragments arranged by size: 3678 3020 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4658 5868 4658 Size: 1210 5556 BclI T'GATC_A Cuts at: 1548 1548 Size: 6766 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3698 3953 5377 Size: 107 122 71 631 605 2026 255 1424 Cuts at: 5377 136 Size: 1525 Fragments arranged by size: 2026 1525 1424 631 605 255 122 107 71 BglII A'GATC_T Cuts at: 2772 6761 2772 Size: 3989 2777 BlpI GC'TnA_GC Cuts at: 4183 4183 Size: 6766 Bme1580I G_kGCm'C Cuts at: 1790 2032 2986 3023 3150 3703 4049 4827 5983 Size: 242 954 37 127 553 346 778 1156 Cuts at: 5983 1790 Size: 2573 Fragments arranged by size: 2573 1156 954 778 553 346 242 127 37 BmgBI CAC'GTC Cuts at: 1318 3843 1318 Size: 2525 4241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3565 4497 5455 Size: 416 53 83 399 24 2148 932 958 Cuts at: 5455 442 Size: 1753 Fragments arranged by size: 2148 1753 958 932 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6766 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3691 3689 Size: 2 6764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2364 2474 3152 3435 3504 3723 3789 4034 Size: 1125 110 678 283 69 219 66 245 Cuts at: 4034 5426 1239 Size: 1392 2579 Fragments arranged by size: 2579 1392 1125 678 283 245 219 110 69 66 Bpu10I CC'TnA_GC Cuts at: 2325 2325 Size: 6766 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4891 5756 6054 4891 Size: 865 298 5603 Fragments arranged by size: 5603 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3403 5429 882 Size: 617 1904 2026 2219 Fragments arranged by size: 2219 2026 1904 617 BsaAI yAC'GTr Cuts at: 493 1204 3184 4063 4557 493 Size: 711 1980 879 494 2702 Fragments arranged by size: 2702 1980 879 711 494 BsaBI GATnn'nnATC Cuts at: 6760 6760 Size: 6766 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3515 4573 4955 5590 Size: 53 83 186 936 1982 1058 382 635 Cuts at: 5590 275 Size: 1451 Fragments arranged by size: 1982 1451 1058 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2108 5199 5597 5871 6018 6429 2108 Size: 3091 398 274 147 411 2445 Fragments arranged by size: 3091 2445 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3602 3632 1178 Size: 30 165 30 2199 30 4312 Fragments arranged by size: 4312 2199 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2157 2532 2594 3610 3674 1186 Size: 889 82 375 62 1016 64 4278 Fragments arranged by size: 4278 1016 889 375 82 64 62 BseYI C'CCAG_C Cuts at: 2677 3163 3684 3763 3984 5969 6465 2677 Size: 486 521 79 221 1985 496 2978 Fragments arranged by size: 2978 1985 521 496 486 221 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2525 2946 1163 Size: 573 789 421 4983 Fragments arranged by size: 4983 789 573 421 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4216 4977 6005 1540 Size: 275 186 2215 761 1028 2301 Fragments arranged by size: 2301 2215 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3926 4827 4912 5983 3926 Size: 901 85 1071 4709 Fragments arranged by size: 4709 1071 901 85 BsmI GAATG_Cn' Cuts at: 3216 3373 3985 4277 4370 3216 Size: 157 612 292 93 5612 Fragments arranged by size: 5612 612 292 157 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2640 3622 4019 2640 Size: 982 397 5387 Fragments arranged by size: 5387 982 397 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2342 2986 3023 3150 3703 3926 Size: 399 242 310 644 37 127 553 223 Cuts at: 3926 4049 4085 4827 4912 5983 1391 Size: 123 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 553 399 310 242 223 127 123 85 37 36 BspEI T'CCGG_A Cuts at: 2108 6429 2108 Size: 4321 2445 BspHI T'CATG_A Cuts at: 4655 6385 4655 Size: 1730 5036 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3249 4121 844 Size: 2405 872 3489 Fragments arranged by size: 3489 2405 872 BsrBI CCG'CTC Cuts at: 1394 1823 4653 1394 Size: 429 2830 3507 Fragments arranged by size: 3507 2830 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2662 5261 5435 65 Size: 1183 1414 2599 174 1396 Fragments arranged by size: 2599 1414 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 3990 5410 5597 1700 Size: 110 230 1950 1420 187 2869 Fragments arranged by size: 2869 1950 1420 230 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6766 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6766 BssSI C'ACGA_G Cuts at: 4826 5838 4826 Size: 1012 5754 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6766 BstBI TT'CG_AA Cuts at: 2007 5570 2007 Size: 3563 3203 BstEII G'GTnAC_C Cuts at: 1184 2174 1184 Size: 990 5776 BstXI CCAn_nnnn'nTGG Cuts at: 4012 4012 Size: 6766 BstYI r'GATC_y Cuts at: 1972 2557 2772 2994 4096 4463 4848 4865 6317 Size: 585 215 222 1102 367 385 17 1452 Cuts at: 6317 6403 6415 6761 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1102 585 385 367 346 222 215 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2436 2553 2631 3585 513 Size: 552 957 414 117 78 954 3694 Fragments arranged by size: 3694 957 954 552 414 117 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3625 3874 487 Size: 683 56 2399 249 3379 Fragments arranged by size: 3379 2399 683 249 56 BtsI GCAGTG_nn' Cuts at: 2984 3235 4282 5076 5096 2984 Size: 251 1047 794 20 4654 Fragments arranged by size: 4654 1047 794 251 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6766 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6731 DraI TTT'AAA Cuts at: 4070 4070 Size: 6766 DraIII CAC_nnn'GTG Cuts at: 2554 2554 Size: 6766 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3520 3968 5773 809 Size: 507 2204 448 1805 1802 Fragments arranged by size: 2204 1805 1802 507 448 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2659 3196 3235 Size: 54 1425 50 275 18 829 537 39 Cuts at: 3235 4213 5102 6731 8 Size: 978 889 1629 43 Fragments arranged by size: 1629 1425 978 889 829 537 275 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 4213 1537 Size: 275 2401 4090 Fragments arranged by size: 4090 2401 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2343 2436 2571 3072 3132 3349 3354 Size: 977 13 93 135 501 60 217 5 Cuts at: 3354 3651 3682 3801 4696 1353 Size: 297 31 119 895 3423 Fragments arranged by size: 3423 977 895 501 297 217 135 119 93 60 31 13 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5353 5727 5873 387 Size: 1064 138 3764 374 146 1280 Fragments arranged by size: 3764 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2364 2474 2750 2841 2975 3096 Size: 393 558 174 110 276 91 134 121 Cuts at: 3096 3152 3435 3504 3675 3723 3789 4034 4829 Size: 56 283 69 171 48 66 245 795 Cuts at: 4829 5426 6213 6481 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 795 787 597 558 393 283 276 268 245 174 171 134 121 110 91 69 66 56 48 EcoNI CCTnn'n_nnAGG Cuts at: 2765 2765 Size: 6766 EcoO109I rG'GnC_Cy Cuts at: 2028 2029 3020 3136 3147 3548 3699 3700 4032 Size: 1 991 116 11 401 151 1 332 Cuts at: 4032 4045 4046 2028 Size: 13 1 4748 Fragments arranged by size: 4748 991 401 332 151 116 13 11 1 1 1 EcoRI G'AATT_C Cuts at: 4075 4453 4075 Size: 378 6388 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2600 2600 Size: 6766 FspI TGC'GCA Cuts at: 3847 5272 3847 Size: 1425 5341 FspAI rTGC'GCAy Cuts at: 3847 3847 Size: 6766 HaeII r_GCGC'y Cuts at: 1678 3991 3997 5913 1678 Size: 2313 6 1916 2531 Fragments arranged by size: 2531 2313 1916 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 3071 3103 3250 Size: 32 135 32 488 32 979 32 147 Cuts at: 3250 3263 3282 3291 3295 3323 3485 3517 3602 Size: 13 19 9 4 28 162 32 85 Cuts at: 3602 3634 3800 3832 5407 5439 5481 5513 1373 Size: 32 166 32 1575 32 42 32 2626 Fragments arranged by size: 2626 1575 979 488 166 162 147 135 85 42 32 32 32 32 32 32 32 32 32 28 19 13 9 4 HincII GTy'rAC Cuts at: 669 1657 1938 4110 4356 669 Size: 988 281 2172 246 3079 Fragments arranged by size: 3079 2172 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6766 HpaI GTT'AAC Cuts at: 4356 4356 Size: 6766 KpnI G_GTAC'C Cuts at: 4091 4091 Size: 6766 MfeI C'AATT_G Cuts at: 4365 4365 Size: 6766 MluI A'CGCG_T Cuts at: 6642 6642 Size: 6766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 3369 3956 5880 6064 1305 Size: 1170 894 587 1924 184 2007 Fragments arranged by size: 2007 1924 1170 894 587 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2661 3198 3237 10 Size: 54 1425 343 829 537 39 3539 Fragments arranged by size: 3539 1425 829 537 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2376 2667 2700 3584 4724 5083 5242 Size: 648 1210 291 33 884 1140 359 159 Cuts at: 5242 518 Size: 2042 Fragments arranged by size: 2042 1210 1140 884 648 359 291 159 33 MspA1I CmG'CkG Cuts at: 1606 3731 3767 4182 4859 6007 6252 1606 Size: 2125 36 415 677 1148 245 2120 Fragments arranged by size: 2125 2120 1148 677 415 245 36 NaeI GCC'GGC Cuts at: 2042 3992 2042 Size: 1950 4816 NcoI C'CATG_G Cuts at: 513 1065 2022 3585 513 Size: 552 957 1563 3694 Fragments arranged by size: 3694 1563 957 552 NdeI CA'TA_TG Cuts at: 387 3223 387 Size: 2836 3930 NgoMIV G'CCGG_C Cuts at: 2040 3990 2040 Size: 1950 4816 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6766 NotI GC'GGCC_GC Cuts at: 4213 4213 Size: 6766 NspI r_CATG'y Cuts at: 1132 1229 2630 4124 5669 1132 Size: 97 1401 1494 1545 2229 Fragments arranged by size: 2229 1545 1494 1401 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6766 PciI A'CATG_T Cuts at: 5665 5665 Size: 6766 PflMI CCAn_nnn'nTGG Cuts at: 1108 2554 1108 Size: 1446 5320 PfoI T'CCnGG_A Cuts at: 2874 2874 Size: 6766 PmeI GTTT'AAAC Cuts at: 4070 4070 Size: 6766 PmlI CAC'GTG Cuts at: 3184 3184 Size: 6766 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4835 4867 6348 6380 4835 Size: 32 1481 32 5221 Fragments arranged by size: 5221 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6766 PsiI TTA'TAA Cuts at: 4336 4336 Size: 6766 PspOMI G'GGCC_C Cuts at: 2028 3019 3146 3699 4045 2028 Size: 991 127 553 346 4749 Fragments arranged by size: 4749 991 553 346 127 PstI C_TGCA'G Cuts at: 830 3383 4118 830 Size: 2553 735 3478 Fragments arranged by size: 3478 2553 735 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6766 PvuII CAG'CTG Cuts at: 3767 3767 Size: 6766 SalI G'TCGA_C Cuts at: 1936 4108 1936 Size: 2172 4594 SapI GCTCTTCn'nnn_ Cuts at: 2330 3072 2330 Size: 742 6024 SbfI CC_TGCA'GG Cuts at: 4118 4118 Size: 6766 ScaI AGT'ACT Cuts at: 5014 6726 5014 Size: 1712 5054 SexAI A'CCwGG_T Cuts at: 3940 3940 Size: 6766 SfcI C'TryA_G Cuts at: 826 1045 3379 4114 5249 5930 6121 826 Size: 219 2334 735 1135 681 191 1471 Fragments arranged by size: 2334 1471 1135 735 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 SgrAI Cr'CCGG_yG Cuts at: 1700 3990 1700 Size: 2290 4476 SmaI CCC'GGG Cuts at: 1872 4093 1872 Size: 2221 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4870 5771 6033 820 Size: 197 926 2927 901 262 1553 Fragments arranged by size: 2927 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4063 4557 493 Size: 3570 494 2702 Fragments arranged by size: 3570 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6766 SphI G_CATG'C Cuts at: 1132 4124 1132 Size: 2992 3774 SspI AAT'ATT Cuts at: 5 52 3100 4690 5 Size: 47 3048 1590 2081 Fragments arranged by size: 3048 2081 1590 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6766 StyI C'CwwG_G Cuts at: 513 1065 2022 3585 4205 513 Size: 552 957 1563 620 3074 Fragments arranged by size: 3074 1563 957 620 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2616 3835 4793 4810 4963 5148 5593 Size: 1255 1036 1219 958 17 153 185 445 Cuts at: 5593 325 Size: 1498 Fragments arranged by size: 1498 1255 1219 1036 958 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 2671 2793 Size: 274 80 33 51 1443 531 163 122 Cuts at: 2793 5012 6724 96 Size: 2219 1712 138 Fragments arranged by size: 2219 1712 1443 531 274 163 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2542 2574 6726 6758 2542 Size: 32 4152 32 2550 Fragments arranged by size: 4152 2550 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2750 3614 1316 Size: 1434 864 4468 Fragments arranged by size: 4468 1434 864 XbaI T'CTAG_A Cuts at: 4102 4102 Size: 6766 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3562 3562 Size: 6766 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6766 XmaI C'CCGG_G Cuts at: 1870 4091 1870 Size: 2221 4545 XmnI GAAnn'nnTTC Cuts at: 4895 4895 Size: 6766 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4574 276 Size: 53 83 186 936 3040 2468 Fragments arranged by size: 3040 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI