Restriction map for
pFN21ASDA1912
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflIII AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AvrII
BalI BamHI BbeI BcgI BciVI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoRI EcoT14I EcoT22I FseI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1222 GTCTAC GT^[AC][GT]AC
AflII 1567 CTTAAG C^TTAAG
AgeI 959 ACCGGT A^CCGGT
AscI 170 GGCGCGCC GG^CGCGCC
AspI 383 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 538 TGCGCA TGC^GCA
BbrPI 922 CACGTG CAC^GTG
BbsI 298 GAAGAC GAAGAC(2/6)
BbvCI 56 CCTCAGC CCTCAGC(-5/-2)
BclI 1110 TGATCA T^GATCA
BfrI 1567 CTTAAG C^TTAAG
BpuAI 298 GAAGAC GAAGAC(2/6)
BsmFI 317 GGGAC GGGAC(10/14)
BssHII 171 GCGCGC G^CGCGC
BstAPI 545 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1202 GGTCACC G^GT[ACGT]ACC
BstPI 1202 GGTCACC G^GT[ACGT]ACC
BstYI 260 AGATCC [GA]^GATC[CT]
EcoNI 1253 CCTATGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1202 GGTCACC G^GT[ACGT]ACC
EcoRV 277 GATATC GAT^ATC
FbaI 1110 TGATCA T^GATCA
FspI 538 TGCGCA TGC^GCA
HaeII 757 AGCGCC [GA]GCGC^[CT]
HinfI 1240 GAATC G^A[ACGT]TC
MflI 260 AGATCC [GA]^GATC[CT]
MseI 1568 TTAA T^TAA
MslI 1213 CACCGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 67 GCCGGC GCC^GGC
NgoMIV 67 GCCGGC G^CCGGC
NheI 1510 GCTAGC G^CTAGC
PflFI 383 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 959 ACCGGT A^CCGGT
PmaCI 922 CACGTG CAC^GTG
PmlI 922 CACGTG CAC^GTG
SgrAI 92 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1449 CCCGGG CCC^GGG
SphI 1304 GCATGC GCATG^C
TfiI 1240 GAATC G^A[AT]TC
Tru9I 1568 TTAA T^TAA
Tsp509I 1617 AATT ^AATT
Tth111I 383 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 681 CCACGCGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 260 AGATCC [GA]^GATC[CT]
XmaCI 1449 CCCGGG C^CCGGG
XmaI 1449 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 1558 GTAC GT^AC
1644 GTAC
AlwI 156 GATCC GGATC(4/5)
261 GATCC
BsaAI 607 TACGTG [CT]AC^GT[GA]
922 CACGTG
BsaWI 959 ACCGGT [AT]^CCGG[AT]
1214 ACCGGA
BsgI 247 CTGCAC GTGCAG(16/14)
530 GTGCAG
BsiEI 11 CGGCCG CG[GA][CT]^CG
65 CGGCCG
BsmAI 381 GAGAC GTCTC(1/5)
366 GTCTC
DsaI 77 CCGCGG C^C[GA][CT]GG
264 CCGCGG
EaeI 11 CGGCCG [CT]^GGCC[GA]
65 CGGCCG
EagI 11 CGGCCG C^GGCCG
65 CGGCCG
EclXI 11 CGGCCG C^GGCCG
65 CGGCCG
Eco52I 11 CGGCCG C^GGCCG
65 CGGCCG
KspI 77 CCGCGG CCGC^GG
264 CCGCGG
MaeII 608 ACGT A^CGT
923 ACGT
NspI 1304 GCATGC [GA]CATG^[CT]
1524 ACATGC
RsaI 1558 GTAC GT^AC
1644 GTAC
SacI 490 GAGCTC GAGCT^C
499 GAGCTC
SacII 77 CCGCGG CCGC^GG
264 CCGCGG
TaqI 4 TCGA T^CGA
560 TCGA
Tsp45I 623 GTGAC ^GT[GC]AC
1203 GTCAC
TthHB8I 4 TCGA T^CGA
560 TCGA
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TseI TspRI