(Circular) (Six-base) MAPSORT of: pFN21ASDA1911.seq Check: 596 from: 1 to: 7249 pFN21ASDA1911 7249 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5059 278 Size: 53 83 186 936 3523 2468 Fragments arranged by size: 3523 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3151 3899 4592 1937 Size: 1214 748 693 4594 Fragments arranged by size: 4594 1214 748 693 Acc65I G'GTAC_C Cuts at: 4570 4570 Size: 7249 AclI AA'CG_TT Cuts at: 1563 5376 5749 1563 Size: 3813 373 3063 Fragments arranged by size: 3813 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2766 3423 4059 5312 6696 6964 1632 Size: 1134 657 636 1253 1384 268 1917 Fragments arranged by size: 1917 1384 1253 1134 657 636 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7052 AflIII A'CryG_T Cuts at: 1203 2542 6148 7125 1203 Size: 1339 3606 977 1327 Fragments arranged by size: 3606 1339 1327 977 AgeI A'CCGG_T Cuts at: 6080 6080 Size: 7249 AhdI GACnn_n'nnGTC Cuts at: 5978 5978 Size: 7249 AleI CACnn'nnGTG Cuts at: 1166 4123 1166 Size: 2957 4292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7249 AlwNI CAG_nnn'CTG Cuts at: 2876 3934 4313 4992 6564 2876 Size: 1058 379 679 1572 3561 Fragments arranged by size: 3561 1572 1058 679 379 ApaLI G'TGCA_C Cuts at: 5306 6462 5306 Size: 1156 6093 ApoI r'AATT_y Cuts at: 1493 2053 2089 2103 2442 2617 3215 3553 3659 Size: 560 36 14 339 175 598 338 106 Cuts at: 3659 3993 4558 4784 4936 7045 1493 Size: 334 565 226 152 2109 1697 Fragments arranged by size: 2109 1697 598 565 560 339 338 334 226 175 152 106 36 14 AseI AT'TA_AT Cuts at: 160 1032 2271 2604 3396 3417 3438 4524 5803 Size: 872 1239 333 792 21 21 1086 1279 Cuts at: 5803 160 Size: 1606 Fragments arranged by size: 1606 1279 1239 1086 872 792 333 21 21 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7249 AvaI C'yCGr_G Cuts at: 1870 1943 4574 1870 Size: 73 2631 4545 Fragments arranged by size: 4545 2631 73 BamHI G'GATC_C Cuts at: 4579 4946 4579 Size: 367 6882 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4570 6025 618 Size: 325 217 627 2783 1455 1842 Fragments arranged by size: 2783 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3460 4568 1391 Size: 2069 1108 4072 Fragments arranged by size: 4072 2069 1108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2717 4047 928 Size: 971 818 1330 4130 Fragments arranged by size: 4130 1330 971 818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2722 2756 3092 3126 5440 5474 1903 Size: 34 785 34 336 34 2314 34 3678 Fragments arranged by size: 3678 2314 785 336 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5141 6351 5141 Size: 1210 6039 BclI T'GATC_A Cuts at: 1548 1548 Size: 7249 BfrBI ATG'CAT Cuts at: 3310 3310 Size: 7249 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5860 136 Size: 107 122 71 631 605 4188 1525 Fragments arranged by size: 4188 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7244 7244 Size: 7249 BlpI GC'TnA_GC Cuts at: 3172 4666 3172 Size: 1494 5755 Bme1580I G_kGCm'C Cuts at: 1790 5310 6466 1790 Size: 3520 1156 2573 Fragments arranged by size: 3520 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7249 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4980 5938 442 Size: 416 53 83 399 24 3563 958 1753 Fragments arranged by size: 3563 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7249 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2074 2074 Size: 7249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2918 5909 1239 Size: 1679 2991 2579 Fragments arranged by size: 2991 2579 1679 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3234 5374 6239 6537 3234 Size: 2140 865 298 3946 Fragments arranged by size: 3946 2140 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3044 5912 882 Size: 617 1545 2868 2219 Fragments arranged by size: 2868 2219 1545 617 BsaAI yAC'GTr Cuts at: 493 1204 4542 4546 5040 493 Size: 711 3338 4 494 2702 Fragments arranged by size: 3338 2702 711 494 4 BsaBI GATnn'nnATC Cuts at: 2592 7243 2592 Size: 4651 2598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5056 5438 6073 275 Size: 53 83 186 936 3523 382 635 1451 Fragments arranged by size: 3523 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2379 5682 6080 6354 6501 6912 2379 Size: 3303 398 274 147 411 2716 Fragments arranged by size: 3303 2716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7024 Fragments arranged by size: 7024 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7249 BseYI C'CCAG_C Cuts at: 6452 6948 6452 Size: 496 6753 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3248 1163 Size: 573 1512 5164 Fragments arranged by size: 5164 1512 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4699 5460 6488 1540 Size: 275 186 2698 761 1028 2301 Fragments arranged by size: 2698 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5310 5395 6466 5310 Size: 85 1071 6093 Fragments arranged by size: 6093 1071 85 BsiWI C'GTAC_G Cuts at: 4542 4542 Size: 7249 BsmI GAATG_Cn' Cuts at: 3897 4394 4760 4853 3897 Size: 497 366 93 6293 Fragments arranged by size: 6293 497 366 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2718 2718 Size: 7249 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3460 4568 5310 5395 6466 1391 Size: 399 1670 1108 742 85 1071 2174 Fragments arranged by size: 2174 1670 1108 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6912 6912 Size: 7249 BspHI T'CATG_A Cuts at: 5138 6868 5138 Size: 1730 5519 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4604 844 Size: 3760 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5136 1394 Size: 429 3313 3507 Fragments arranged by size: 3507 3313 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3110 5744 5918 65 Size: 1183 1862 2634 174 1396 Fragments arranged by size: 2634 1862 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5893 6080 1700 Size: 110 4083 187 2869 Fragments arranged by size: 4083 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7249 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7249 BssSI C'ACGA_G Cuts at: 5309 6321 5309 Size: 1012 6237 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7249 BstBI TT'CG_AA Cuts at: 2007 6053 2007 Size: 4046 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7249 BstYI r'GATC_y Cuts at: 1972 2690 4450 4579 4946 5331 5348 6800 6886 Size: 718 1760 129 367 385 17 1452 86 Cuts at: 6886 6898 7244 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1760 1452 718 385 367 346 129 86 17 12 BstZ17I GTA'TAC Cuts at: 3152 3900 3152 Size: 748 6501 Bsu36I CC'TnA_GG Cuts at: 4394 4394 Size: 7249 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6697 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6510 Fragments arranged by size: 6510 683 56 BtsI GCAGTG_nn' Cuts at: 2504 3724 4765 5559 5579 2504 Size: 1220 1041 794 20 4174 Fragments arranged by size: 4174 1220 1041 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7249 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7214 DraI TTT'AAA Cuts at: 4553 4553 Size: 7249 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6256 809 Size: 507 4940 1802 Fragments arranged by size: 4940 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3477 4696 5585 Size: 54 1425 50 275 18 1647 1219 889 Cuts at: 5585 7214 8 Size: 1629 43 Fragments arranged by size: 1647 1629 1425 1219 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4696 1537 Size: 275 2884 4090 Fragments arranged by size: 4090 2884 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 2716 3042 4157 4221 5179 1353 Size: 1190 173 326 1115 64 958 3423 Fragments arranged by size: 3423 1190 1115 958 326 173 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5836 6210 6356 387 Size: 1064 138 4247 374 146 1280 Fragments arranged by size: 4247 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2766 2918 3423 4059 5312 5909 6696 Size: 393 1134 152 505 636 1253 597 787 Cuts at: 6696 6964 1239 Size: 268 1524 Fragments arranged by size: 1524 1253 1134 787 636 597 505 393 268 152 EcoNI CCTnn'n_nnAGG Cuts at: 3264 4061 3264 Size: 797 6452 EcoRI G'AATT_C Cuts at: 2053 2089 3993 4558 4936 2053 Size: 36 1904 565 378 4366 Fragments arranged by size: 4366 1904 565 378 36 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4250 4250 Size: 7249 FspI TGC'GCA Cuts at: 5755 5755 Size: 7249 HaeII r_GCGC'y Cuts at: 1678 6396 1678 Size: 4718 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3527 3559 5890 5922 5964 Size: 32 135 32 1955 32 2331 32 42 Cuts at: 5964 5996 1373 Size: 32 2626 Fragments arranged by size: 2626 2331 1955 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4069 4090 4593 4839 669 Size: 988 281 2131 21 503 246 3079 Fragments arranged by size: 3079 2131 988 503 281 246 21 HindIII A'AGCT_T Cuts at: 748 748 Size: 7249 HpaI GTT'AAC Cuts at: 4839 4839 Size: 7249 KpnI G_GTAC'C Cuts at: 4574 4574 Size: 7249 MfeI C'AATT_G Cuts at: 4848 4848 Size: 7249 MluI A'CGCG_T Cuts at: 7125 7125 Size: 7249 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3213 3222 4340 6363 6547 1305 Size: 1908 9 1118 2023 184 2007 Fragments arranged by size: 2023 2007 1908 1118 184 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 3479 10 Size: 54 1425 343 1647 3780 Fragments arranged by size: 3780 1647 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4123 5207 5566 5725 518 Size: 648 2957 1084 359 159 2042 Fragments arranged by size: 2957 2042 1084 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4665 5342 6490 6735 1606 Size: 3059 677 1148 245 2120 Fragments arranged by size: 3059 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6697 NdeI CA'TA_TG Cuts at: 387 387 Size: 7249 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7249 NotI GC'GGCC_GC Cuts at: 4696 4696 Size: 7249 NsiI A_TGCA'T Cuts at: 3312 3312 Size: 7249 NspI r_CATG'y Cuts at: 1132 1229 2546 4607 6152 1132 Size: 97 1317 2061 1545 2229 Fragments arranged by size: 2229 2061 1545 1317 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7249 PciI A'CATG_T Cuts at: 2542 6148 2542 Size: 3606 3643 PflMI CCAn_nnn'nTGG Cuts at: 1108 3568 1108 Size: 2460 4789 PmeI GTTT'AAAC Cuts at: 4553 4553 Size: 7249 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5318 5350 6831 6863 5318 Size: 32 1481 32 5704 Fragments arranged by size: 5704 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7249 PsiI TTA'TAA Cuts at: 3956 4819 3956 Size: 863 6386 PstI C_TGCA'G Cuts at: 830 4601 830 Size: 3771 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7249 SalI G'TCGA_C Cuts at: 1936 4591 1936 Size: 2655 4594 SbfI CC_TGCA'GG Cuts at: 4601 4601 Size: 7249 ScaI AGT'ACT Cuts at: 2509 2559 4487 5497 7209 2509 Size: 50 1928 1010 1712 2549 Fragments arranged by size: 2549 1928 1712 1010 50 SfcI C'TryA_G Cuts at: 826 1045 4213 4597 5732 6413 6604 826 Size: 219 3168 384 1135 681 191 1471 Fragments arranged by size: 3168 1471 1135 681 384 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7249 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7249 SmaI CCC'GGG Cuts at: 1872 4576 1872 Size: 2704 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3249 5353 6254 6516 820 Size: 197 926 1306 2104 901 262 1553 Fragments arranged by size: 2104 1553 1306 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4546 5040 493 Size: 4053 494 2702 Fragments arranged by size: 4053 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7249 SphI G_CATG'C Cuts at: 1132 4607 1132 Size: 3475 3774 SspI AAT'ATT Cuts at: 5 52 5173 5 Size: 47 5121 2081 Fragments arranged by size: 5121 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7249 StyI C'CwwG_G Cuts at: 513 1065 4688 513 Size: 552 3623 3074 Fragments arranged by size: 3623 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5276 5293 5446 5631 6076 325 Size: 1255 3696 17 153 185 445 1498 Fragments arranged by size: 3696 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2222 2344 2507 Size: 274 80 33 51 1443 245 122 163 Cuts at: 2507 2557 3329 4485 5495 7207 96 Size: 50 772 1156 1010 1712 138 Fragments arranged by size: 1712 1443 1156 1010 772 274 245 163 138 122 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7209 7241 7209 Size: 32 7217 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7249 XbaI T'CTAG_A Cuts at: 4585 4585 Size: 7249 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7249 XmaI C'CCGG_G Cuts at: 1870 4574 1870 Size: 2704 4545 XmnI GAAnn'nnTTC Cuts at: 3745 3993 5378 3745 Size: 248 1385 5616 Fragments arranged by size: 5616 1385 248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5057 276 Size: 53 83 186 936 3523 2468 Fragments arranged by size: 3523 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BstXI DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI