Restriction map for
pFN21ASDA1899
(Linear) (Six-base) MAPSORT of: KIAA1899.seq Check: 9551 from: 1 to: 3072
KIAA1899.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1313 1744 3072
Size: 1313 431 1328
Fragments arranged by size:
1328 1313 431
AccI GT'mk_AC
Cuts at: 0 557 3072
Size: 557 2515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 515 1082 1687 1726 2333 3072
Size: 126 389 567 605 39 607 739
Fragments arranged by size:
739 607 605 567 389 126 39
AflII C'TTAA_G
Cuts at: 0 528 2655 3072
Size: 528 2127 417
Fragments arranged by size:
2127 528 417
AflIII A'CryG_T
Cuts at: 0 1535 2216 3072
Size: 1535 681 856
Fragments arranged by size:
1535 856 681
AgeI A'CCGG_T
Cuts at: 0 2816 3072
Size: 2816 256
AleI CACnn'nnGTG
Cuts at: 0 1642 3072
Size: 1642 1430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1711 1907 3072
Size: 1711 196 1165
Fragments arranged by size:
1711 1165 196
AlwNI CAG_nnn'CTG
Cuts at: 0 1704 3072
Size: 1704 1368
ApaI G_GGCC'C
Cuts at: 0 1101 1622 3072
Size: 1101 521 1450
Fragments arranged by size:
1450 1101 521
ApaLI G'TGCA_C
Cuts at: 0 1441 3072
Size: 1441 1631
ApoI r'AATT_y
Cuts at: 0 200 211 268 548 2172 2456 2783 3072
Size: 200 11 57 280 1624 284 327 289
Fragments arranged by size:
1624 327 289 284 280 200 57 11
BamHI G'GATC_C
Cuts at: 0 600 3013 3072
Size: 600 2413 59
Fragments arranged by size:
2413 600 59
BanI G'GyrC_C
Cuts at: 0 1206 1448 1462 3072
Size: 1206 242 14 1610
Fragments arranged by size:
1610 1206 242 14
BanII G_rGCy'C
Cuts at: 0 65 1101 1622 3072
Size: 65 1036 521 1450
Fragments arranged by size:
1450 1036 521 65
BbeI G_GCGC'C
Cuts at: 0 1466 3072
Size: 1466 1606
BbsI GAAGACnn'nnnn_
Cuts at: 0 1801 3072
Size: 1801 1271
BbvCI CC'TCA_GC
Cuts at: 0 2401 3072
Size: 2401 671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1683 1717 1972 2006 2623 2657 3072
Size: 1683 34 255 34 617 34 415
Fragments arranged by size:
1683 617 415 255 34 34 34
BclI T'GATC_A
Cuts at: 0 516 3072
Size: 516 2556
BfrBI ATG'CAT
Cuts at: 0 2673 3072
Size: 2673 399
Bme1580I G_kGCm'C
Cuts at: 0 1101 1445 1451 1622 2920 3072
Size: 1101 344 6 171 1298 152
Fragments arranged by size:
1298 1101 344 171 152 6
BmgBI CAC'GTC
Cuts at: 0 54 2219 2326 3072
Size: 54 2165 107 746
Fragments arranged by size:
2165 746 107 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 284 435 684 3072
Size: 284 151 249 2388
Fragments arranged by size:
2388 284 249 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2683 3072
Size: 2683 389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 964 1274 1608 2752 3072
Size: 70 894 310 334 1144 320
Fragments arranged by size:
1144 894 334 320 310 70
Bpu10I CC'TnA_GC
Cuts at: 0 2401 3072
Size: 2401 671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 3072
Size: 1210 1862
BsaBI GATnn'nnATC
Cuts at: 0 2768 3072
Size: 2768 304
BsaHI Gr'CG_yC
Cuts at: 0 1463 3072
Size: 1463 1609
BsaWI w'CCGG_w
Cuts at: 0 2816 3072
Size: 2816 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1050 1080 3072
Size: 1050 30 1992
Fragments arranged by size:
1992 1050 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2207 3072
Size: 2207 865
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 3072
Size: 1219 1853
BsiHKAI G_wGCw'C
Cuts at: 0 65 1445 1732 3072
Size: 65 1380 287 1340
Fragments arranged by size:
1380 1340 287 65
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1377 3072
Size: 1377 1695
Bsp1286I G_dGCh'C
Cuts at: 0 65 1101 1445 1451 1622 1732 2920 3072
Size: 65 1036 344 6 171 110 1188 152
Fragments arranged by size:
1188 1036 344 171 152 110 65 6
BspHI T'CATG_A
Cuts at: 0 1923 2702 2861 3072
Size: 1923 779 159 211
Fragments arranged by size:
1923 779 211 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1313 1424 1721 1744 3072
Size: 1313 111 297 23 1328
Fragments arranged by size:
1328 1313 297 111 23
BsrDI GCAATG_nn'
Cuts at: 0 1889 3072
Size: 1889 1183
BsrFI r'CCGG_y
Cuts at: 0 79 2816 3072
Size: 79 2737 256
Fragments arranged by size:
2737 256 79
BsrGI T'GTAC_A
Cuts at: 0 749 1103 1319 1797 3072
Size: 749 354 216 478 1275
Fragments arranged by size:
1275 749 478 354 216
BssSI C'ACGA_G
Cuts at: 0 1981 3072
Size: 1981 1091
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1736 3072
Size: 1736 1336
BstYI r'GATC_y
Cuts at: 0 600 2062 3013 3072
Size: 600 1462 951 59
Fragments arranged by size:
1462 951 600 59
BtgI C'CryG_G
Cuts at: 0 19 1640 2416 2423 3072
Size: 19 1621 776 7 649
Fragments arranged by size:
1621 776 649 19 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2626 3072
Size: 2626 446
BtsI GCAGTG_nn'
Cuts at: 0 762 1614 1723 1930 3072
Size: 762 852 109 207 1142
Fragments arranged by size:
1142 852 762 207 109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1175 1210 2476 2511 3072
Size: 1175 35 1266 35 561
Fragments arranged by size:
1266 1175 561 35 35
DraI TTT'AAA
Cuts at: 0 2961 2993 3072
Size: 2961 32 79
Fragments arranged by size:
2961 79 32
DraIII CAC_nnn'GTG
Cuts at: 0 1242 3072
Size: 1242 1830
EaeI y'GGCC_r
Cuts at: 0 960 3072
Size: 960 2112
EarI CTCTTCn'nnn_
Cuts at: 0 247 500 3072
Size: 247 253 2572
Fragments arranged by size:
2572 253 247
EcoICRI GAG'CTC
Cuts at: 0 63 3072
Size: 63 3009
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 126 515 964 1082 1274 1608 1687
Size: 70 56 389 449 118 192 334 79
Cuts at: 1687 1726 2333 2752 3072
Size: 39 607 419 320
Fragments arranged by size:
607 449 419 389 334 320 192 118
79 70 56 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1321 1626 3072
Size: 1321 305 1446
Fragments arranged by size:
1446 1321 305
EcoO109I rG'GnC_Cy
Cuts at: 0 683 1098 1618 1619 2412 3072
Size: 683 415 520 1 793 660
Fragments arranged by size:
793 683 660 520 415 1
EcoRI G'AATT_C
Cuts at: 0 548 3072
Size: 548 2524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1150 2491 2590 2859 3072
Size: 1150 1341 99 269 213
Fragments arranged by size:
1341 1150 269 213 99
HaeII r_GCGC'y
Cuts at: 0 1466 3072
Size: 1466 1606
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2005 2037 2054 2086 2822 2854 3072
Size: 2005 32 17 32 736 32 218
Fragments arranged by size:
2005 736 218 32 32 32 17
HincII GTy'rAC
Cuts at: 0 168 1058 2352 2804 3072
Size: 168 890 1294 452 268
Fragments arranged by size:
1294 890 452 268 168
KasI G'GCGC_C
Cuts at: 0 1462 3072
Size: 1462 1610
MfeI C'AATT_G
Cuts at: 0 2773 3072
Size: 2773 299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 161 229 595 1329 2304 3072
Size: 11 150 68 366 734 975 768
Fragments arranged by size:
975 768 734 366 150 68 11
MscI TGG'CCA
Cuts at: 0 962 3072
Size: 962 2110
MslI CAynn'nnrTG
Cuts at: 0 1642 2866 3072
Size: 1642 1224 206
Fragments arranged by size:
1642 1224 206
MspA1I CmG'CkG
Cuts at: 0 1701 3072
Size: 1701 1371
NaeI GCC'GGC
Cuts at: 0 81 3072
Size: 81 2991
NarI GG'CG_CC
Cuts at: 0 1463 3072
Size: 1463 1609
NcoI C'CATG_G
Cuts at: 0 1640 2416 3072
Size: 1640 776 656
Fragments arranged by size:
1640 776 656
NdeI CA'TA_TG
Cuts at: 0 957 2972 3072
Size: 957 2015 100
Fragments arranged by size:
2015 957 100
NgoMIV G'CCGG_C
Cuts at: 0 79 3072
Size: 79 2993
NsiI A_TGCA'T
Cuts at: 0 2675 3072
Size: 2675 397
NspI r_CATG'y
Cuts at: 0 1026 2039 2649 3072
Size: 1026 1013 610 423
Fragments arranged by size:
1026 1013 610 423
PfoI T'CCnGG_A
Cuts at: 0 86 980 3072
Size: 86 894 2092
Fragments arranged by size:
2092 894 86
PpuMI rG'GwC_Cy
Cuts at: 0 2412 3072
Size: 2412 660
PshAI GACnn'nnGTC
Cuts at: 0 464 2819 3072
Size: 464 2355 253
Fragments arranged by size:
2355 464 253
PsiI TTA'TAA
Cuts at: 0 2437 2462 3072
Size: 2437 25 610
Fragments arranged by size:
2437 610 25
PspOMI G'GGCC_C
Cuts at: 0 1097 1618 3072
Size: 1097 521 1454
Fragments arranged by size:
1454 1097 521
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1250 1282 3072
Size: 1250 32 1790
Fragments arranged by size:
1790 1250 32
PstI C_TGCA'G
Cuts at: 0 1421 1718 2550 3072
Size: 1421 297 832 522
Fragments arranged by size:
1421 832 522 297
PvuII CAG'CTG
Cuts at: 0 1701 3072
Size: 1701 1371
SacI G_AGCT'C
Cuts at: 0 65 3072
Size: 65 3007
ScaI AGT'ACT
Cuts at: 0 670 1251 2544 3072
Size: 670 581 1293 528
Fragments arranged by size:
1293 670 581 528
SfcI C'TryA_G
Cuts at: 0 1417 1714 2546 2790 2936 3006 3072
Size: 1417 297 832 244 146 70 66
Fragments arranged by size:
1417 832 297 244 146 70 66
SfoI GGC'GCC
Cuts at: 0 1464 3072
Size: 1464 1608
SmlI C'TyrA_G
Cuts at: 0 528 1225 2655 3072
Size: 528 697 1430 417
Fragments arranged by size:
1430 697 528 417
SphI G_CATG'C
Cuts at: 0 1026 3072
Size: 1026 2046
SspI AAT'ATT
Cuts at: 0 897 1111 3072
Size: 897 214 1961
Fragments arranged by size:
1961 897 214
StuI AGG'CCT
Cuts at: 0 2573 2912 3072
Size: 2573 339 160
Fragments arranged by size:
2573 339 160
StyI C'CwwG_G
Cuts at: 0 1640 1862 2416 2759 3072
Size: 1640 222 554 343 313
Fragments arranged by size:
1640 554 343 313 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 650 1383 3072
Size: 650 733 1689
Fragments arranged by size:
1689 733 650
TatI w'GTAC_w
Cuts at: 0 668 749 1103 1249 1319 1797 2542 2722
Size: 668 81 354 146 70 478 745 180
Cuts at: 2722 3072
Size: 350
Fragments arranged by size:
745 668 478 354 350 180 146 81
70
XbaI T'CTAG_A
Cuts at: 0 2097 3072
Size: 2097 975
XmnI GAAnn'nnTTC
Cuts at: 0 2176 3072
Size: 2176 896
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PfoI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuII SacI ScaI SfcI SfoI SmlI
SphI SspI StuI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AhdI AloI AscI AseI
AsiSI AvaI AvrII BaeI BciVI BglI BglII BlpI
BmtI BsaI BsaAI BseYI BsiEI BsiWI BsmI BspEI
BsrBI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI
DrdI EagI EciI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MluI NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TstI Tth111I
XcmI XhoI XmaI ZraI