(Circular) (Six-base) MAPSORT of: pFN21ASDA1896.seq Check: 2769 from: 1 to: 6232 pFN21ASDA1896 6232 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3186 3486 3575 1937 Size: 1249 300 89 4594 Fragments arranged by size: 4594 1249 300 89 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3050 3518 4295 5679 5947 1632 Size: 1418 468 777 1384 268 1917 Fragments arranged by size: 1917 1418 1384 777 468 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2784 3237 1184 Size: 1600 453 4179 Fragments arranged by size: 4179 1600 453 AlwNI CAG_nnn'CTG Cuts at: 2035 2126 2401 2724 3285 3975 5547 2035 Size: 91 275 323 561 690 1572 2720 Fragments arranged by size: 2720 1572 690 561 323 275 91 ApaI G_GGCC'C Cuts at: 2483 2483 Size: 6232 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 2294 2445 2897 3541 3767 3919 6028 1493 Size: 801 151 452 644 226 152 2109 1697 Fragments arranged by size: 2109 1697 801 644 452 226 152 151 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2573 3557 1870 Size: 73 630 984 4545 Fragments arranged by size: 4545 984 630 73 BamHI G'GATC_C Cuts at: 3093 3562 3929 3093 Size: 469 367 5396 Fragments arranged by size: 5396 469 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2114 3269 3553 5008 Size: 325 217 627 286 41 1155 284 1455 Cuts at: 5008 618 Size: 1842 Fragments arranged by size: 1842 1455 1155 627 325 286 284 217 41 BanII G_rGCy'C Cuts at: 1391 2483 3551 1391 Size: 1092 1068 4072 Fragments arranged by size: 4072 1092 1068 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3038 3059 928 Size: 971 1139 21 4101 Fragments arranged by size: 4101 1139 971 21 BbvCI CC'TCA_GC Cuts at: 2178 2330 2178 Size: 152 6080 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2725 4124 5334 2725 Size: 1399 1210 3623 Fragments arranged by size: 3623 1399 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 2483 4293 5449 1790 Size: 693 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 693 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3963 4921 442 Size: 416 53 83 399 24 2546 958 1753 Fragments arranged by size: 2546 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2055 1056 Size: 999 5233 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2152 2277 4892 1239 Size: 829 84 125 2615 2579 Fragments arranged by size: 2615 2579 829 125 84 Bpu10I CC'TnA_GC Cuts at: 2178 2330 2178 Size: 152 6080 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2435 2732 3182 4357 5222 5520 2435 Size: 297 450 1175 865 298 3147 Fragments arranged by size: 3147 1175 865 450 298 297 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2130 4895 882 Size: 617 631 2765 2219 Fragments arranged by size: 2765 2219 631 617 BsaAI yAC'GTr Cuts at: 493 1204 3481 3529 4023 493 Size: 711 2277 48 494 2702 Fragments arranged by size: 2702 2277 711 494 48 BsaBI GATnn'nnATC Cuts at: 2516 6226 2516 Size: 3710 2522 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3361 4665 5063 5337 5484 5895 3361 Size: 1304 398 274 147 411 3698 Fragments arranged by size: 3698 1304 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2540 2669 1186 Size: 1354 129 4749 Fragments arranged by size: 4749 1354 129 BseYI C'CCAG_C Cuts at: 2069 2119 3148 3460 5435 5931 2069 Size: 50 1029 312 1975 496 2370 Fragments arranged by size: 2370 1975 1029 496 312 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2710 3536 1163 Size: 573 974 826 3859 Fragments arranged by size: 3859 974 826 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 3682 4443 5471 1540 Size: 275 186 100 1581 761 1028 2301 Fragments arranged by size: 2301 1581 1028 761 275 186 100 BsiHKAI G_wGCw'C Cuts at: 2757 3258 4293 4378 5449 2757 Size: 501 1035 85 1071 3540 Fragments arranged by size: 3540 1071 1035 501 85 BsmI GAATG_Cn' Cuts at: 2596 3078 3208 3743 3836 2596 Size: 482 130 535 93 4992 Fragments arranged by size: 4992 535 482 130 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3185 3185 Size: 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2483 2757 3258 3551 4293 4378 5449 Size: 399 693 274 501 293 742 85 1071 Cuts at: 5449 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 693 501 399 293 274 85 BspEI T'CCGG_A Cuts at: 3361 5895 3361 Size: 2534 3698 BspHI T'CATG_A Cuts at: 2317 3447 4121 5851 2317 Size: 1130 674 1730 2698 Fragments arranged by size: 2698 1730 1130 674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2230 2732 2752 3587 844 Size: 1386 502 20 835 3489 Fragments arranged by size: 3489 1386 835 502 20 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2860 4727 4901 65 Size: 1183 1612 1867 174 1396 Fragments arranged by size: 1867 1612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3167 4876 5063 1700 Size: 110 1357 1709 187 2869 Fragments arranged by size: 2869 1709 1357 187 110 BsrGI T'GTAC_A Cuts at: 96 2248 3423 96 Size: 2152 1175 2905 Fragments arranged by size: 2905 2152 1175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 2960 4292 5304 2960 Size: 1332 1012 3888 Fragments arranged by size: 3888 1332 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2401 794 Size: 1607 4625 BstBI TT'CG_AA Cuts at: 2007 5036 2007 Size: 3029 3203 BstEII G'GTnAC_C Cuts at: 1184 3367 1184 Size: 2183 4049 BstXI CCAn_nnnn'nTGG Cuts at: 2209 3148 2209 Size: 939 5293 BstYI r'GATC_y Cuts at: 1972 3093 3562 3929 4314 4331 5783 5869 5881 Size: 1121 469 367 385 17 1452 86 12 Cuts at: 5881 6227 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1121 469 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2077 2184 2559 513 Size: 552 1012 107 375 4186 Fragments arranged by size: 4186 1012 552 375 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2980 487 Size: 683 56 1754 3739 Fragments arranged by size: 3739 1754 683 56 BtsI GCAGTG_nn' Cuts at: 3231 3748 4542 4562 3231 Size: 517 794 20 4901 Fragments arranged by size: 4901 794 517 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2590 2625 529 Size: 35 2026 35 4136 Fragments arranged by size: 4136 2026 35 35 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DraIII CAC_nnn'GTG Cuts at: 2560 2689 2560 Size: 129 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2414 3182 5239 809 Size: 507 1098 768 2057 1802 Fragments arranged by size: 2057 1802 1098 768 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3099 3114 3289 Size: 54 1425 50 275 18 1269 15 175 Cuts at: 3289 3297 3679 4568 6197 8 Size: 8 382 889 1629 43 Fragments arranged by size: 1629 1425 1269 889 382 275 175 54 50 43 18 15 8 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3389 4162 1353 Size: 2036 773 3423 Fragments arranged by size: 3423 2036 773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2152 2277 3050 3518 4295 4892 Size: 393 436 84 125 773 468 777 597 Cuts at: 4892 5679 5947 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 777 773 597 468 436 393 268 125 84 EcoO109I rG'GnC_Cy Cuts at: 2266 2479 2480 2979 3025 3324 3411 2266 Size: 213 1 499 46 299 87 5087 Fragments arranged by size: 5087 499 299 213 87 46 1 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 EcoRV GAT'ATC Cuts at: 2424 2424 Size: 6232 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2926 3047 3360 5379 1678 Size: 1248 121 313 2019 2531 Fragments arranged by size: 2531 2019 1248 313 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4873 4905 4947 4979 1373 Size: 32 135 32 3301 32 42 32 2626 Fragments arranged by size: 3301 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3822 669 Size: 988 281 1638 246 3079 Fragments arranged by size: 3079 1638 988 281 246 HindIII A'AGCT_T Cuts at: 748 2149 748 Size: 1401 4831 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2197 5346 5530 1305 Size: 892 3149 184 2007 Fragments arranged by size: 3149 2007 892 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3101 3291 3299 10 Size: 54 1425 343 1269 190 8 2943 Fragments arranged by size: 2943 1425 1269 343 190 54 8 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2813 4190 4549 4708 518 Size: 648 1326 321 1377 359 159 2042 Fragments arranged by size: 2042 1377 1326 648 359 321 159 MspA1I CmG'CkG Cuts at: 1606 3295 3464 3648 4325 5473 5718 1606 Size: 1689 169 184 677 1148 245 2120 Fragments arranged by size: 2120 1689 1148 677 245 184 169 NaeI GCC'GGC Cuts at: 3169 3169 Size: 6232 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5680 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NgoMIV G'CCGG_C Cuts at: 3167 3167 Size: 6232 NheI G'CTAG_C Cuts at: 1052 2051 1052 Size: 999 5233 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3148 3590 5135 1132 Size: 97 1919 442 1545 2229 Fragments arranged by size: 2229 1919 1545 442 97 PasI CC'CwG_GG Cuts at: 1348 2064 3508 1348 Size: 716 1444 4072 Fragments arranged by size: 4072 1444 716 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 2523 2560 1108 Size: 1415 37 4780 Fragments arranged by size: 4780 1415 37 PfoI T'CCnGG_A Cuts at: 2634 2634 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2266 2979 3025 3324 2266 Size: 713 46 299 5174 Fragments arranged by size: 5174 713 299 46 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 2479 2479 Size: 6232 PstI C_TGCA'G Cuts at: 830 2227 3216 3584 830 Size: 1397 989 368 3478 Fragments arranged by size: 3478 1397 989 368 PvuI CG_AT'CG Cuts at: 2001 2101 2001 Size: 100 6132 PvuII CAG'CTG Cuts at: 3295 3464 3295 Size: 169 6063 RsrII CG'GwC_CG Cuts at: 3402 3402 Size: 6232 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 3065 4480 6192 3065 Size: 1415 1712 3105 Fragments arranged by size: 3105 1712 1415 SexAI A'CCwGG_T Cuts at: 2685 2685 Size: 6232 SfcI C'TryA_G Cuts at: 826 1045 2223 3212 3580 4715 5396 5587 826 Size: 219 1178 989 368 1135 681 191 1471 Fragments arranged by size: 1471 1178 1135 989 681 368 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2450 2747 3197 4336 5237 5499 Size: 197 926 507 297 450 1139 901 262 Cuts at: 5499 820 Size: 1553 Fragments arranged by size: 1553 1139 926 901 507 450 297 262 197 SnaBI TAC'GTA Cuts at: 493 3529 4023 493 Size: 3036 494 2702 Fragments arranged by size: 3036 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2982 3671 513 Size: 552 1917 689 3074 Fragments arranged by size: 3074 1917 689 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 2606 3419 4259 4276 4429 4614 Size: 1255 481 545 813 840 17 153 185 Cuts at: 4614 5059 325 Size: 445 1498 Fragments arranged by size: 1498 1255 840 813 545 481 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 3063 3423 Size: 274 80 33 51 1443 271 815 360 Cuts at: 3423 4478 6190 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1443 1055 815 360 274 271 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2127 2206 2209 2899 3094 3109 3307 2127 Size: 79 3 690 195 15 198 5052 Fragments arranged by size: 5052 690 198 195 79 15 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 2445 4361 2445 Size: 1916 4316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SapI SfiI SfoI SrfI SwaI