Restriction map for
pFN21ASDA1886
(Circular) (Six-base) MAPSORT of: pFN21ASDA1886.seq Check: 4172 from: 1 to: 7489
pFN21ASDA1886 7489 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5299 278
Size: 53 83 186 936 3763 2468
Fragments arranged by size:
3763 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3387 4412 4832 1937
Size: 1450 1025 420 4594
Fragments arranged by size:
4594 1450 1025 420
Acc65I G'GTAC_C
Cuts at: 4810 4810
Size: 7489
AclI AA'CG_TT
Cuts at: 1563 5616 5989 1563
Size: 4053 373 3063
Fragments arranged by size:
4053 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 3578 3721 3836 4142 4386 4588 5552
Size: 696 1250 143 115 306 244 202 964
Cuts at: 5552 6936 7204 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1250 964 696 306 268 244
202 143 115
AfeI AGC'GCT
Cuts at: 4127 4127
Size: 7489
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7292
AflIII A'CryG_T
Cuts at: 1203 3180 6388 7365 1203
Size: 1977 3208 977 1327
Fragments arranged by size:
3208 1977 1327 977
AgeI A'CCGG_T
Cuts at: 6320 6320
Size: 7489
AhdI GACnn_n'nnGTC
Cuts at: 2813 3895 6218 2813
Size: 1082 2323 4084
Fragments arranged by size:
4084 2323 1082
AleI CACnn'nnGTG
Cuts at: 1166 2153 4619 1166
Size: 987 2466 4036
Fragments arranged by size:
4036 2466 987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2319 2934 1184
Size: 1135 615 5739
Fragments arranged by size:
5739 1135 615
AlwNI CAG_nnn'CTG
Cuts at: 2287 2471 2498 2557 2608 2758 2800 2857 2980
Size: 184 27 59 51 150 42 57 123
Cuts at: 2980 4238 5232 6804 2287
Size: 1258 994 1572 2972
Fragments arranged by size:
2972 1572 1258 994 184 150 123 59
57 51 42 27
ApaI G_GGCC'C
Cuts at: 2210 2374 2684 3771 4196 4510 4632 4727 2210
Size: 164 310 1087 425 314 122 95 4972
Fragments arranged by size:
4972 1087 425 314 310 164 122 95
ApaLI G'TGCA_C
Cuts at: 3644 5546 6702 3644
Size: 1902 1156 4431
Fragments arranged by size:
4431 1902 1156
ApoI r'AATT_y
Cuts at: 1493 4798 5024 5176 7285 1493
Size: 3305 226 152 2109 1697
Fragments arranged by size:
3305 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6043 160
Size: 872 5011 1606
Fragments arranged by size:
5011 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7489
AvaI C'yCGr_G
Cuts at: 1870 1943 2404 2625 2718 3109 3855 4348 4814
Size: 73 461 221 93 391 746 493 466
Cuts at: 4814 1870
Size: 4545
Fragments arranged by size:
4545 746 493 466 461 391 221 93
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2756 2789 2756
Size: 33 7456
BamHI G'GATC_C
Cuts at: 3840 4819 5186 3840
Size: 979 367 6143
Fragments arranged by size:
6143 979 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 3041 3746 3758 4438
Size: 325 217 627 528 726 705 12 680
Cuts at: 4438 4810 6265 618
Size: 372 1455 1842
Fragments arranged by size:
1842 1455 726 705 680 627 528 372
325 217 12
BanII G_rGCy'C
Cuts at: 1391 2210 2374 2684 3327 3771 4145 4196 4510
Size: 819 164 310 643 444 374 51 314
Cuts at: 4510 4632 4727 4808 1391
Size: 122 95 81 4072
Fragments arranged by size:
4072 819 643 444 374 314 310 164
122 95 81 51
BbeI G_GCGC'C
Cuts at: 2319 3045 2319
Size: 726 6763
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3358 928
Size: 971 1459 5059
Fragments arranged by size:
5059 1459 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5680 5714 1903
Size: 34 3743 34 3678
Fragments arranged by size:
3743 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2784 2850 5381 6591 2784
Size: 66 2531 1210 3682
Fragments arranged by size:
3682 2531 1210 66
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7489
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2358 2409 2525
Size: 107 122 71 631 605 686 51 116
Cuts at: 2525 2688 3081 3576 4111 4655 6100 136
Size: 163 393 495 535 544 1445 1525
Fragments arranged by size:
1525 1445 686 631 605 544 535 495
393 163 122 116 107 71 51
BglII A'GATC_T
Cuts at: 7484 7484
Size: 7489
BlpI GC'TnA_GC
Cuts at: 2558 3705 4906 2558
Size: 1147 1201 5141
Fragments arranged by size:
5141 1201 1147
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2374 2684 2946 3648 3771 3824 4074
Size: 420 164 310 262 702 123 53 250
Cuts at: 4074 4196 4510 4632 4727 5550 6706 1790
Size: 122 314 122 95 823 1156 2573
Fragments arranged by size:
2573 1156 823 702 420 314 310 262
250 164 123 122 122 95 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7489
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2884 2968 3114
Size: 416 53 83 399 24 1467 84 146
Cuts at: 3114 5220 6178 442
Size: 2106 958 1753
Fragments arranged by size:
2106 1753 1467 958 416 399 146 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 3341 3437 3914 3986 3995 4497 4665
Size: 1280 822 96 477 72 9 502 168
Cuts at: 4665 6149 1239
Size: 1484 2579
Fragments arranged by size:
2579 1484 1280 822 502 477 168 96
72 9
Bpu10I CC'TnA_GC
Cuts at: 4579 4579
Size: 7489
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 5614 6479 6777 3209
Size: 2405 865 298 3921
Fragments arranged by size:
3921 2405 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6152 882
Size: 617 4653 2219
Fragments arranged by size:
4653 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2332 4053 4153 4786 5280 493
Size: 711 1128 1721 100 633 494 2702
Fragments arranged by size:
2702 1721 1128 711 633 494 100
BsaBI GATnn'nnATC
Cuts at: 4040 7483 4040
Size: 3443 4046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 3042 5296 5678
Size: 53 83 186 936 783 726 2254 382
Cuts at: 5678 6313 275
Size: 635 1451
Fragments arranged by size:
2254 1451 936 783 726 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5922 6320 6594 6741 7152 5922
Size: 398 274 147 411 6259
Fragments arranged by size:
6259 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3224 3254 4719 4749 1178
Size: 30 165 30 1821 30 1465 30 3918
Fragments arranged by size:
3918 1821 1465 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3671 4046 4115 1186
Size: 2485 375 69 4560
Fragments arranged by size:
4560 2485 375 69
BseYI C'CCAG_C
Cuts at: 2793 2879 2900 3994 4163 4244 4319 4385 4521
Size: 86 21 1094 169 81 75 66 136
Cuts at: 4521 4546 4634 6692 7188 2793
Size: 25 88 2058 496 3094
Fragments arranged by size:
3094 2058 1094 496 169 136 88 86
81 75 66 25 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 3503 4075 4511 1163
Size: 573 618 1149 572 436 4141
Fragments arranged by size:
4141 1149 618 573 572 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4939 5700 6728 1540
Size: 275 186 2938 761 1028 2301
Fragments arranged by size:
2938 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3648 3711 4158 5550 5635 6706 3648
Size: 63 447 1392 85 1071 4431
Fragments arranged by size:
4431 1392 1071 447 85 63
BsmI GAATG_Cn'
Cuts at: 5000 5093 5000
Size: 93 7396
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2374 2684 2946 3327 3648 3711
Size: 399 420 164 310 262 381 321 63
Cuts at: 3711 3771 3824 4074 4145 4158 4196 4510 4632
Size: 60 53 250 71 13 38 314 122
Cuts at: 4632 4727 4808 5550 5635 6706 1391
Size: 95 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 381 321 314
310 262 250 164 122 95 85 81
71 63 60 53 38 13
BspEI T'CCGG_A
Cuts at: 7152 7152
Size: 7489
BspHI T'CATG_A
Cuts at: 3862 5378 7108 3862
Size: 1516 1730 4243
Fragments arranged by size:
4243 1730 1516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2056 3475 3912 4436 4686 4767 4844 844
Size: 1212 1419 437 524 250 81 77 3489
Fragments arranged by size:
3489 1419 1212 524 437 250 81 77
BsrBI CCG'CTC
Cuts at: 1394 1823 3508 3663 3922 5376 1394
Size: 429 1685 155 259 1454 3507
Fragments arranged by size:
3507 1685 1454 429 259 155
BsrDI GCAATG_nn'
Cuts at: 65 1248 5984 6158 65
Size: 1183 4736 174 1396
Fragments arranged by size:
4736 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 3450 6133 6320 1700
Size: 110 315 1325 2683 187 2869
Fragments arranged by size:
2869 2683 1325 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 2346 96
Size: 2250 5239
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7489
BssSI C'ACGA_G
Cuts at: 3443 5549 6561 3443
Size: 2106 1012 4371
Fragments arranged by size:
4371 2106 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2471 2498 2758 2857 2950 3643 794
Size: 1677 27 260 99 93 693 4640
Fragments arranged by size:
4640 1677 693 260 99 93 27
BstBI TT'CG_AA
Cuts at: 2007 6293 2007
Size: 4286 3203
BstEII G'GTnAC_C
Cuts at: 1184 4448 1184
Size: 3264 4225
BstXI CCAn_nnnn'nTGG
Cuts at: 4171 4276 4171
Size: 105 7384
BstYI r'GATC_y
Cuts at: 1972 3840 4819 5186 5571 5588 7040 7126 7138
Size: 1868 979 367 385 17 1452 86 12
Cuts at: 7138 7484 1972
Size: 346 1977
Fragments arranged by size:
1977 1868 1452 979 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2202 2353 2523 2997 513
Size: 552 1137 151 170 474 5005
Fragments arranged by size:
5005 1137 552 474 170 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2588 3237 3321 487
Size: 683 56 1362 649 84 4655
Fragments arranged by size:
4655 1362 683 649 84 56
BtsI GCAGTG_nn'
Cuts at: 2496 2798 3128 3709 5005 5799 5819 2496
Size: 302 330 581 1296 794 20 4166
Fragments arranged by size:
4166 1296 794 581 330 302 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7454
DraI TTT'AAA
Cuts at: 4793 4793
Size: 7489
DraIII CAC_nnn'GTG
Cuts at: 2072 2072
Size: 7489
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6496 809
Size: 507 5180 1802
Fragments arranged by size:
5180 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3470 4258 4936
Size: 54 1425 50 275 18 1640 788 678
Cuts at: 4936 5825 7454 8
Size: 889 1629 43
Fragments arranged by size:
1640 1629 1425 889 788 678 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4936 1537
Size: 275 3124 4090
Fragments arranged by size:
4090 3124 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3632 5419 1353
Size: 2279 1787 3423
Fragments arranged by size:
3423 2279 1787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2254 3320 6076 6450 6596 387
Size: 1064 138 665 1066 2756 374 146 1280
Fragments arranged by size:
2756 1280 1066 1064 665 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2519 3341 3437 3578 3721 3836
Size: 393 696 191 822 96 141 143 115
Cuts at: 3836 3914 3986 3995 4142 4386 4497 4588 4665
Size: 78 72 9 147 244 111 91 77
Cuts at: 4665 5552 6149 6936 7204 1239
Size: 887 597 787 268 1524
Fragments arranged by size:
1524 887 822 787 696 597 393 268
244 191 147 143 141 115 111 96
91 78 77 72 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2960 3835 3855 4614 2960
Size: 875 20 759 5835
Fragments arranged by size:
5835 875 759 20
EcoO109I rG'GnC_Cy
Cuts at: 2207 2370 2371 2410 2621 2630 2681 3676 3970
Size: 163 1 39 211 9 51 995 294
Cuts at: 3970 4193 4280 4506 4507 4517 4583 4628 4629
Size: 223 87 226 1 10 66 45 1
Cuts at: 4629 4657 2207
Size: 28 5039
Fragments arranged by size:
5039 995 294 226 223 211 163 87
66 51 45 39 28 10 9 1
1 1
EcoRI G'AATT_C
Cuts at: 4798 5176 4798
Size: 378 7111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3226 3288 3751 3226
Size: 62 463 6964
Fragments arranged by size:
6964 463 62
FspI TGC'GCA
Cuts at: 5995 5995
Size: 7489
HaeII r_GCGC'y
Cuts at: 1678 2118 2319 3045 3694 4129 4203 6636 1678
Size: 440 201 726 649 435 74 2433 2531
Fragments arranged by size:
2531 2433 726 649 440 435 201 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2799 2831 3188 3220 3369
Size: 32 135 32 1227 32 357 32 149
Cuts at: 3369 3401 3881 3913 4697 4729 6130 6162 6204
Size: 32 480 32 784 32 1401 32 42
Cuts at: 6204 6236 1373
Size: 32 2626
Fragments arranged by size:
2626 1401 1227 784 480 357 149 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4833 5079 669
Size: 988 281 2895 246 3079
Fragments arranged by size:
3079 2895 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7489
HpaI GTT'AAC
Cuts at: 5079 5079
Size: 7489
KasI G'GCGC_C
Cuts at: 2315 3041 2315
Size: 726 6763
KpnI G_GTAC'C
Cuts at: 4814 4814
Size: 7489
MfeI C'AATT_G
Cuts at: 5088 5088
Size: 7489
MluI A'CGCG_T
Cuts at: 7365 7365
Size: 7489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4521 6603 6787 1305
Size: 3216 2082 184 2007
Fragments arranged by size:
3216 2082 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3472 4260 10
Size: 54 1425 343 1640 788 3239
Fragments arranged by size:
3239 1640 1425 788 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2153 4619 5447 5806 5965 518
Size: 648 907 80 2466 828 359 159 2042
Fragments arranged by size:
2466 2042 907 828 648 359 159 80
MspA1I CmG'CkG
Cuts at: 1606 2355 2468 2477 2645 3373 3744 4059 4525
Size: 749 113 9 168 728 371 315 466
Cuts at: 4525 4905 5582 6730 6975 1606
Size: 380 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 728 677 466 380 371
315 245 168 113 9
NaeI GCC'GGC
Cuts at: 2127 2127
Size: 7489
NarI GG'CG_CC
Cuts at: 2316 3042 2316
Size: 726 6763
NcoI C'CATG_G
Cuts at: 513 1065 2202 513
Size: 552 1137 5800
Fragments arranged by size:
5800 1137 552
NdeI CA'TA_TG
Cuts at: 387 2089 387
Size: 1702 5787
NgoMIV G'CCGG_C
Cuts at: 2125 2125
Size: 7489
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7489
NotI GC'GGCC_GC
Cuts at: 4936 4936
Size: 7489
NspI r_CATG'y
Cuts at: 1132 1229 2432 3184 3566 3994 4090 4642 4847
Size: 97 1203 752 382 428 96 552 205
Cuts at: 4847 6392 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1203 752 552 428 382 205
97 96
PasI CC'CwG_GG
Cuts at: 1348 2366 2906 2939 3764 4189 1348
Size: 1018 540 33 825 425 4648
Fragments arranged by size:
4648 1018 825 540 425 33
PciI A'CATG_T
Cuts at: 3180 6388 3180
Size: 3208 4281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2191 1108
Size: 1083 6406
PfoI T'CCnGG_A
Cuts at: 2535 3318 4683 2535
Size: 783 1365 5341
Fragments arranged by size:
5341 1365 783
PmeI GTTT'AAAC
Cuts at: 4793 4793
Size: 7489
PmlI CAC'GTG
Cuts at: 4053 4153 4053
Size: 100 7389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5558 5590 7071 7103 5558
Size: 32 1481 32 5944
Fragments arranged by size:
5944 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2621 4517 4583 2621
Size: 1896 66 5527
Fragments arranged by size:
5527 1896 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7489
PsiI TTA'TAA
Cuts at: 3012 5059 3012
Size: 2047 5442
PspOMI G'GGCC_C
Cuts at: 2206 2370 2680 3767 4192 4506 4628 4723 2206
Size: 164 310 1087 425 314 122 95 4972
Fragments arranged by size:
4972 1087 425 314 310 164 122 95
PstI C_TGCA'G
Cuts at: 830 2215 2476 2613 2961 3339 3639 3675 3909
Size: 1385 261 137 348 378 300 36 234
Cuts at: 3909 4224 4841 830
Size: 315 617 3478
Fragments arranged by size:
3478 1385 617 378 348 315 300 261
234 137 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7489
PvuII CAG'CTG
Cuts at: 2468 2477 3373 4059 4525 2468
Size: 9 896 686 466 5432
Fragments arranged by size:
5432 896 686 466 9
SacII CC_GC'GG
Cuts at: 2356 2356
Size: 7489
SalI G'TCGA_C
Cuts at: 1936 4831 1936
Size: 2895 4594
SbfI CC_TGCA'GG
Cuts at: 2215 4841 2215
Size: 2626 4863
ScaI AGT'ACT
Cuts at: 5737 7449 5737
Size: 1712 5777
SfcI C'TryA_G
Cuts at: 826 1045 2211 2472 2609 2957 3070 3335 3635
Size: 219 1166 261 137 348 113 265 300
Cuts at: 3635 3671 3905 4220 4837 5972 6653 6844 826
Size: 36 234 315 617 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 681 617 348 315 300
265 261 234 219 191 137 113 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 2409 2688 2409
Size: 279 7210
SfoI GGC'GCC
Cuts at: 2317 3043 2317
Size: 726 6763
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7489
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7489
SmaI CCC'GGG
Cuts at: 1872 2406 2627 2720 3111 4350 4816 1872
Size: 534 221 93 391 1239 466 4545
Fragments arranged by size:
4545 1239 534 466 391 221 93
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 5593 6494 6756 820
Size: 197 926 1281 2369 901 262 1553
Fragments arranged by size:
2369 1553 1281 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4786 5280 493
Size: 4293 494 2702
Fragments arranged by size:
4293 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7489
SphI G_CATG'C
Cuts at: 1132 4847 1132
Size: 3715 3774
SspI AAT'ATT
Cuts at: 5 52 5413 5
Size: 47 5361 2081
Fragments arranged by size:
5361 2081 47
StuI AGG'CCT
Cuts at: 1517 3409 1517
Size: 1892 5597
StyI C'CwwG_G
Cuts at: 513 1065 2102 2202 2700 4501 4563 4928 513
Size: 552 1037 100 498 1801 62 365 3074
Fragments arranged by size:
3074 1801 1037 552 498 365 100 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 3166 4324 5516 5533 5686 5871
Size: 1255 1313 273 1158 1192 17 153 185
Cuts at: 5871 6316 325
Size: 445 1498
Fragments arranged by size:
1498 1313 1255 1192 1158 445 273 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 3987 5735
Size: 274 80 33 51 1443 369 1641 1748
Cuts at: 5735 7447 96
Size: 1712 138
Fragments arranged by size:
1748 1712 1641 1443 369 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 4453 4485 4719 4751 7449 7481 3638
Size: 32 783 32 234 32 2698 32 3646
Fragments arranged by size:
3646 2698 783 234 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2974 3383 1316
Size: 1658 409 5422
Fragments arranged by size:
5422 1658 409
XbaI T'CTAG_A
Cuts at: 4825 4825
Size: 7489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2521 4621 2521
Size: 2100 5389
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7489
XmaI C'CCGG_G
Cuts at: 1870 2404 2625 2718 3109 4348 4814 1870
Size: 534 221 93 391 1239 466 4545
Fragments arranged by size:
4545 1239 534 466 391 221 93
XmnI GAAnn'nnTTC
Cuts at: 5618 5618
Size: 7489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5297 276
Size: 53 83 186 936 3763 2468
Fragments arranged by size:
3763 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SanDI SapI
SexAI SrfI SwaI