Restriction map for
pFN21ASDA1882
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AocI AscI AseI AsnI Asp718I
AviII AvrII BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SexAI SfiI
SfuI SnaBI SpeI SspBI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 1402 GTCTAC GT^[AC][GT]AC
AcyI 3039 GGCGCC G[GA]^CG[CT]C
AgeI 1590 ACCGGT A^CCGGT
AhdI 1477 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1157 AGCGCT AGC^GCT
AspEI 1477 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3235 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1329 GGATCC G^GATCC
BbeI 3039 GGCGCC GGCGC^C
BbvCI 28 GCTGAGG CCTCAGC(-5/-2)
BcgI 2475 GCAGGTCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 3039 GGCGCC G[GA]^CG[CT]C
BspLU11I 680 ACATGT A^CATGT
BsrDI 2126 CATTGC GCAATG(2/0)
Eam1105I 1477 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1157 AGCGCT AGC^GCT
EcoNI 1409 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 3039 GGCGCC G[GA]^CG[CT]C
HincII 1695 GTCAAC GT[CT]^[GA]AC
HindII 1695 GTCAAC GT[CT]^[GA]AC
KasI 3039 GGCGCC G^GCGCC
NaeI 2794 GCCGGC GCC^GGC
NarI 3039 GGCGCC GG^CGCC
NcoI 1550 CCATGG C^CATGG
NgoMIV 2794 GCCGGC G^CCGGC
NheI 1648 GCTAGC G^CTAGC
PflFI 3235 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1590 ACCGGT A^CCGGT
ScaI 922 AGTACT AGT^ACT
SfoI 3039 GGCGCC GGC^GCC
SmaI 2595 CCCGGG CCC^GGG
SmlI 2298 CTTGAG C^T[CT][GA]AG
SphI 611 GCATGC GCATG^C
SspI 2218 AATATT AAT^ATT
StuI 2922 AGGCCT AGG^CCT
Tth111I 3235 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2595 CCCGGG C^CCGGG
XmaI 2595 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 3214 GTGCAC G^TGCAC
3415 GTGCAC
ApaI 3166 GGGCCC GGGCC^C
3311 GGGCCC
ApaLI 3214 GTGCAC G^TGCAC
3415 GTGCAC
BalI 274 TGGCCA TGG^CCA
2354 TGGCCA
BglI 3104 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3427 GCCCTGGAGGC
BglII 311 AGATCT A^GATCT
2113 AGATCT
BsmBI 75 CGTCTC CGTCTC(1/5)
3240 CGTCTC
BsmI 1014 GAATGC GAATGC(1/-1)
1107 GAATGC
BsrBI 1750 GAGCGG CCGCTC(-3/-3)
2905 GAGCGG
BssSI 3361 CACGAG CACGAG(-5/-1)
49 CTCGTG
BstEII 1981 GGTTACC G^GT[ACGT]ACC
2250 GGTCACC
BstPI 1981 GGTTACC G^GT[ACGT]ACC
2250 GGTCACC
EcoO65I 1981 GGTTACC G^GT[ACGT]ACC
2250 GGTCACC
HindIII 1606 AAGCTT A^AGCTT
1674 AAGCTT
MluNI 274 TGGCCA TGG^CCA
2354 TGGCCA
MscI 274 TGGCCA TGG^CCA
2354 TGGCCA
MseI 1121 TTAA T^TAA
2207 TTAA
PpuMI 629 GGGACCT [GA]G^G[AT]CC[CT]
1946 GGGTCCC
PshAI 1445 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1935 GACGCTCGTC
SacI 800 GAGCTC GAGCT^C
1177 GAGCTC
SapI 1747 GAAGAGC GCTCTTC(1/4)
2902 GAAGAGC
SbfI 639 CCTGCAGG CCTGCA^GG
2412 CCTGCAGG
SgrAI 1589 CACCGGTG C[GA]^CCGG[CT]G
2793 CGCCGGCG
Sse8387I 639 CCTGCAGG CCTGCA^GG
2412 CCTGCAGG
Tru9I 1121 TTAA T^TAA
2207 TTAA
XcmI 1235 CCATGCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2172 CCAGCTTTACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI