Restriction map for
pFN21ASDA1882
(Linear) (Six-base) MAPSORT of: KIAA1882.seq Check: 5036 from: 1 to: 3444
KIAA1882.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2723 3038 3444
Size: 2723 315 406
Fragments arranged by size:
2723 406 315
AccI GT'mk_AC
Cuts at: 0 1403 3444
Size: 1403 2041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 854 980 1087 2627 2992 3444
Size: 78 776 126 107 1540 365 452
Fragments arranged by size:
1540 776 452 365 126 107 78
AfeI AGC'GCT
Cuts at: 0 1159 3444
Size: 1159 2285
AflIII A'CryG_T
Cuts at: 0 680 1056 1691 3419 3444
Size: 680 376 635 1728 25
Fragments arranged by size:
1728 680 635 376 25
AgeI A'CCGG_T
Cuts at: 0 1590 3444
Size: 1590 1854
AhdI GACnn_n'nnGTC
Cuts at: 0 1482 3444
Size: 1482 1962
AleI CACnn'nnGTG
Cuts at: 0 391 1739 3422 3444
Size: 391 1348 1683 22
Fragments arranged by size:
1683 1348 391 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2122 3400 3440 3444
Size: 2122 1278 40 4
Fragments arranged by size:
2122 1278 40 4
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1516 1548 3444
Size: 1516 32 1896
Fragments arranged by size:
1896 1516 32
AlwNI CAG_nnn'CTG
Cuts at: 0 45 757 1364 2015 3444
Size: 45 712 607 651 1429
Fragments arranged by size:
1429 712 651 607 45
ApaI G_GGCC'C
Cuts at: 0 3170 3315 3444
Size: 3170 145 129
Fragments arranged by size:
3170 145 129
ApaLI G'TGCA_C
Cuts at: 0 3214 3415 3444
Size: 3214 201 29
Fragments arranged by size:
3214 201 29
ApoI r'AATT_y
Cuts at: 0 1096 1151 2574 3444
Size: 1096 55 1423 870
Fragments arranged by size:
1423 1096 870 55
AvaI C'yCGr_G
Cuts at: 0 187 2489 2518 2595 2672 2918 3307 3444
Size: 187 2302 29 77 77 246 389 137
Fragments arranged by size:
2302 389 246 187 137 77 77 29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2147 2180 3444
Size: 2147 33 1264
Fragments arranged by size:
2147 1264 33
BamHI G'GATC_C
Cuts at: 0 1329 3444
Size: 1329 2115
BanI G'GyrC_C
Cuts at: 0 173 599 886 1593 3039 3444
Size: 173 426 287 707 1446 405
Fragments arranged by size:
1446 707 426 405 287 173
BanII G_rGCy'C
Cuts at: 0 804 1181 1559 3154 3170 3256 3315 3444
Size: 804 377 378 1595 16 86 59 129
Fragments arranged by size:
1595 804 378 377 129 86 59 16
BbeI G_GCGC'C
Cuts at: 0 3043 3444
Size: 3043 401
BbsI GAAGACnn'nnnn_
Cuts at: 0 342 968 1989 2560 2721 3444
Size: 342 626 1021 571 161 723
Fragments arranged by size:
1021 723 626 571 342 161
BbvCI CC'TCA_GC
Cuts at: 0 29 3444
Size: 29 3415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2464 2498 3444
Size: 2464 34 946
Fragments arranged by size:
2464 946 34
BglI GCCn_nnn'nGGC
Cuts at: 0 3110 3433 3444
Size: 3110 323 11
Fragments arranged by size:
3110 323 11
BglII A'GATC_T
Cuts at: 0 311 2113 3444
Size: 311 1802 1331
Fragments arranged by size:
1802 1331 311
Bme1580I G_kGCm'C
Cuts at: 0 864 891 1598 3170 3218 3315 3419 3429
Size: 864 27 707 1572 48 97 104 10
Cuts at: 3429 3444
Size: 15
Fragments arranged by size:
1572 864 707 104 97 48 27 15
10
BmgBI CAC'GTC
Cuts at: 0 393 937 3444
Size: 393 544 2507
Fragments arranged by size:
2507 544 393
BmrI ACTGGGnnnn_n'
Cuts at: 0 1924 2322 3404 3444
Size: 1924 398 1082 40
Fragments arranged by size:
1924 1082 398 40
BmtI G_CTAG'C
Cuts at: 0 1652 3444
Size: 1652 1792
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 818 2271 3444
Size: 818 1453 1173
Fragments arranged by size:
1453 1173 818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 2266 2609 2917 2947 2976 3043 3148
Size: 496 1770 343 308 30 29 67 105
Cuts at: 3148 3240 3444
Size: 92 204
Fragments arranged by size:
1770 496 343 308 204 105 92 67
30 29
Bpu10I CC'TnA_GC
Cuts at: 0 29 952 3444
Size: 29 923 2492
Fragments arranged by size:
2492 923 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2319 3444
Size: 2319 1125
BsaAI yAC'GTr
Cuts at: 0 300 624 1057 2550 3420 3444
Size: 300 324 433 1493 870 24
Fragments arranged by size:
1493 870 433 324 300 24
BsaHI Gr'CG_yC
Cuts at: 0 3040 3444
Size: 3040 404
BsaWI w'CCGG_w
Cuts at: 0 1590 3146 3353 3444
Size: 1590 1556 207 91
Fragments arranged by size:
1590 1556 207 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1255 1285 1867 1897 3226 3256 3444
Size: 1255 30 582 30 1329 30 188
Fragments arranged by size:
1329 1255 582 188 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 1282 3307 3444
Size: 97 1185 2025 137
Fragments arranged by size:
2025 1185 137 97
BseYI C'CCAG_C
Cuts at: 0 146 1364 1680 2688 2943 3444
Size: 146 1218 316 1008 255 501
Fragments arranged by size:
1218 1008 501 316 255 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 2102 2773 3214 3301 3444
Size: 322 1780 671 441 87 143
Fragments arranged by size:
1780 671 441 322 143 87
BsiHKAI G_wGCw'C
Cuts at: 0 804 1181 3029 3218 3419 3444
Size: 804 377 1848 189 201 25
Fragments arranged by size:
1848 804 377 201 189 25
BsmI GAATG_Cn'
Cuts at: 0 1020 1113 3444
Size: 1020 93 2331
Fragments arranged by size:
2331 1020 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 81 3246 3444
Size: 81 3165 198
Fragments arranged by size:
3165 198 81
Bsp1286I G_dGCh'C
Cuts at: 0 804 864 891 1181 1559 1598 3029 3154
Size: 804 60 27 290 378 39 1431 125
Cuts at: 3154 3170 3218 3256 3315 3419 3429 3444
Size: 16 48 38 59 104 10 15
Fragments arranged by size:
1431 804 378 290 125 104 60 59
48 39 38 27 16 15 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2039 2466 2723 3038 3236 3444
Size: 2039 427 257 315 198 208
Fragments arranged by size:
2039 427 315 257 208 198
BsrBI CCG'CTC
Cuts at: 0 1752 2907 3444
Size: 1752 1155 537
Fragments arranged by size:
1752 1155 537
BsrDI GCAATG_nn'
Cuts at: 0 2125 3444
Size: 2125 1319
BsrFI r'CCGG_y
Cuts at: 0 851 1590 2794 3444
Size: 851 739 1204 650
Fragments arranged by size:
1204 851 739 650
BssSI C'ACGA_G
Cuts at: 0 49 3361 3444
Size: 49 3312 83
Fragments arranged by size:
3312 83 49
BstEII G'GTnAC_C
Cuts at: 0 1981 2250 3444
Size: 1981 269 1194
Fragments arranged by size:
1981 1194 269
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 1872 2895 3395 3444
Size: 489 1383 1023 500 49
Fragments arranged by size:
1383 1023 500 489 49
BstYI r'GATC_y
Cuts at: 0 98 311 521 1329 2113 2418 2930 3444
Size: 98 213 210 808 784 305 512 514
Fragments arranged by size:
808 784 514 512 305 213 210 98
BtgI C'CryG_G
Cuts at: 0 1550 1740 1991 3444
Size: 1550 190 251 1453
Fragments arranged by size:
1550 1453 251 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1186 1242 1545 3444
Size: 1186 56 303 1899
Fragments arranged by size:
1899 1186 303 56
BtsI GCAGTG_nn'
Cuts at: 0 28 1246 2713 2985 3055 3444
Size: 28 1218 1467 272 70 389
Fragments arranged by size:
1467 1218 389 272 70 28
EaeI y'GGCC_r
Cuts at: 0 263 274 853 1229 1657 2354 3291 3444
Size: 263 11 579 376 428 697 937 153
Fragments arranged by size:
937 697 579 428 376 263 153 11
EarI CTCTTCn'nnn_
Cuts at: 0 430 787 1139 1742 1910 2897 3325 3444
Size: 430 357 352 603 168 987 428 119
Fragments arranged by size:
987 603 430 428 357 352 168 119
EcoICRI GAG'CTC
Cuts at: 0 802 1179 3444
Size: 802 377 2265
Fragments arranged by size:
2265 802 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 496 854 980 1087 2266 2609 2627
Size: 78 418 358 126 107 1179 343 18
Cuts at: 2627 2917 2947 2976 2992 3043 3148 3240 3444
Size: 290 30 29 16 51 105 92 204
Fragments arranged by size:
1179 418 358 343 290 204 126 107
105 92 78 51 30 29 18 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1413 3444
Size: 1413 2031
EcoO109I rG'GnC_Cy
Cuts at: 0 630 1277 1947 3166 3167 3311 3312 3444
Size: 630 647 670 1219 1 144 1 132
Fragments arranged by size:
1219 670 647 630 144 132 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2853 3444
Size: 2853 591
HaeII r_GCGC'y
Cuts at: 0 1161 1900 3043 3444
Size: 1161 739 1143 401
Fragments arranged by size:
1161 1143 739 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 1468 1500 1867 1899 2078 2110
Size: 244 32 1192 32 367 32 179 32
Cuts at: 2110 2137 2169 3226 3258 3444
Size: 27 32 1057 32 186
Fragments arranged by size:
1192 1057 367 244 186 179 32 32
32 32 32 32 27
HincII GTy'rAC
Cuts at: 0 1697 3444
Size: 1697 1747
HindIII A'AGCT_T
Cuts at: 0 1606 1674 3444
Size: 1606 68 1770
Fragments arranged by size:
1770 1606 68
KasI G'GCGC_C
Cuts at: 0 3039 3444
Size: 3039 405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1466 3444
Size: 1466 1978
MscI TGG'CCA
Cuts at: 0 276 2356 3444
Size: 276 2080 1088
Fragments arranged by size:
2080 1088 276
MslI CAynn'nnrTG
Cuts at: 0 391 685 1739 2377 3422 3444
Size: 391 294 1054 638 1045 22
Fragments arranged by size:
1054 1045 638 391 294 22
MspA1I CmG'CkG
Cuts at: 0 1806 1868 2895 3036 3444
Size: 1806 62 1027 141 408
Fragments arranged by size:
1806 1027 408 141 62
NaeI GCC'GGC
Cuts at: 0 2796 3444
Size: 2796 648
NarI GG'CG_CC
Cuts at: 0 3040 3444
Size: 3040 404
NcoI C'CATG_G
Cuts at: 0 1550 3444
Size: 1550 1894
NgoMIV G'CCGG_C
Cuts at: 0 2794 3444
Size: 2794 650
NheI G'CTAG_C
Cuts at: 0 1648 3444
Size: 1648 1796
NspI r_CATG'y
Cuts at: 0 21 615 684 1627 2644 3444
Size: 21 594 69 943 1017 800
Fragments arranged by size:
1017 943 800 594 69 21
PasI CC'CwG_GG
Cuts at: 0 2631 2860 3444
Size: 2631 229 584
Fragments arranged by size:
2631 584 229
PciI A'CATG_T
Cuts at: 0 680 3444
Size: 680 2764
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 272 429 1398 2811 3444
Size: 66 206 157 969 1413 633
Fragments arranged by size:
1413 969 633 206 157 66
PfoI T'CCnGG_A
Cuts at: 0 1295 2720 2849 3020 3194 3444
Size: 1295 1425 129 171 174 250
Fragments arranged by size:
1425 1295 250 174 171 129
PmlI CAC'GTG
Cuts at: 0 300 624 2550 3420 3444
Size: 300 324 1926 870 24
Fragments arranged by size:
1926 870 324 300 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 3444
Size: 448 32 2964
Fragments arranged by size:
2964 448 32
PpuMI rG'GwC_Cy
Cuts at: 0 630 1947 3444
Size: 630 1317 1497
Fragments arranged by size:
1497 1317 630
PshAI GACnn'nnGTC
Cuts at: 0 1449 1939 3444
Size: 1449 490 1505
Fragments arranged by size:
1505 1449 490
PspOMI G'GGCC_C
Cuts at: 0 3166 3311 3444
Size: 3166 145 133
Fragments arranged by size:
3166 145 133
PstI C_TGCA'G
Cuts at: 0 362 644 659 2093 2417 3035 3050 3444
Size: 362 282 15 1434 324 618 15 394
Fragments arranged by size:
1434 618 394 362 324 282 15 15
PvuII CAG'CTG
Cuts at: 0 1806 2895 3036 3444
Size: 1806 1089 141 408
Fragments arranged by size:
1806 1089 408 141
SacI G_AGCT'C
Cuts at: 0 804 1181 3444
Size: 804 377 2263
Fragments arranged by size:
2263 804 377
SanDI GG'GwC_CC
Cuts at: 0 1947 3444
Size: 1947 1497
SapI GCTCTTCn'nnn_
Cuts at: 0 1742 2897 3444
Size: 1742 1155 547
Fragments arranged by size:
1742 1155 547
SbfI CC_TGCA'GG
Cuts at: 0 644 2417 3444
Size: 644 1773 1027
Fragments arranged by size:
1773 1027 644
ScaI AGT'ACT
Cuts at: 0 924 3444
Size: 924 2520
SfcI C'TryA_G
Cuts at: 0 168 358 640 655 1161 1314 1462 2089
Size: 168 190 282 15 506 153 148 627
Cuts at: 2089 2413 2541 3031 3046 3444
Size: 324 128 490 15 398
Fragments arranged by size:
627 506 490 398 324 282 190 168
153 148 128 15 15
SfoI GGC'GCC
Cuts at: 0 3041 3444
Size: 3041 403
SgrAI Cr'CCGG_yG
Cuts at: 0 1590 2794 3444
Size: 1590 1204 650
Fragments arranged by size:
1590 1204 650
SmaI CCC'GGG
Cuts at: 0 2597 3444
Size: 2597 847
SmlI C'TyrA_G
Cuts at: 0 2298 3444
Size: 2298 1146
SphI G_CATG'C
Cuts at: 0 615 3444
Size: 615 2829
SrfI GCCC'GGGC
Cuts at: 0 2597 3444
Size: 2597 847
SspI AAT'ATT
Cuts at: 0 2220 3444
Size: 2220 1224
StuI AGG'CCT
Cuts at: 0 2924 3444
Size: 2924 520
StyI C'CwwG_G
Cuts at: 0 266 603 1550 3444
Size: 266 337 947 1894
Fragments arranged by size:
1894 947 337 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1259 3444
Size: 1259 2185
TatI w'GTAC_w
Cuts at: 0 922 3444
Size: 922 2522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 730 762 1255 1287 1593 1625 2426 2458
Size: 730 32 493 32 306 32 801 32
Cuts at: 2458 3444
Size: 986
Fragments arranged by size:
986 801 730 493 306 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3238 3444
Size: 3238 206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1242 2179 3444
Size: 1242 937 1265
Fragments arranged by size:
1265 1242 937
XmaI C'CCGG_G
Cuts at: 0 2595 3444
Size: 2595 849
XmnI GAAnn'nnTTC
Cuts at: 0 91 1100 2069 3444
Size: 91 1009 969 1375
Fragments arranged by size:
1375 1009 969 91
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SapI SbfI ScaI SfcI SfoI SgrAI
SmaI SmlI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AscI AseI AsiSI AvrII
BciVI BclI BfrBI BlpI BsaI BsaBI BsiEI BsiWI
BspEI BspHI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI
NdeI NotI NruI NsiI PacI PmeI PsiI PspXI
PsrI PvuI RsrII SacII SalI SexAI SfiI SgfI
SnaBI SpeI SwaI XbaI XhoI ZraI