Restriction map for
pFN21ASDA1872
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbrPI
BcgI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MspA1I MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 23 TCCGGA T^CCGGA
AcsI 153 AAATTT [GA]^AATT[CT]
AcyI 452 GGCGCC G[GA]^CG[CT]C
AflIII 936 ACACGT A^C[GA][CT]GT
Alw44I 895 GTGCAC G^TGCAC
ApaLI 895 GTGCAC G^TGCAC
ApoI 153 AAATTT [GA]^AATT[CT]
AspI 952 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 391 CCCGGG C^[CT]CG[GA]G
BanII 667 GAGCTC G[GA]GC[CT]^C
BbeI 452 GGCGCC GGCGC^C
BbvCI 679 GCTGAGG CCTCAGC(-5/-2)
BciVI 255 GGATAC GTATCC(6/5)
BglII 66 AGATCT A^GATCT
BsaAI 892 TACGTG [CT]AC^GT[GA]
BsaHI 452 GGCGCC G[GA]^CG[CT]C
BseAI 23 TCCGGA T^CCGGA
BseRI 137 CTCCTC GAGGAG(10/8)
BsgI 502 CTGCAC GTGCAG(16/14)
BsiEI 771 CGACCG CG[GA][CT]^CG
BsmBI 91 GAGACG CGTCTC(1/5)
BsoBI 391 CCCGGG C^[CT]CG[GA]G
BspEI 23 TCCGGA T^CCGGA
BspMI 500 ACCTGC ACCTGC(4/8)
BssSI 819 CTCGTG CACGAG(-5/-1)
BstEII 531 GGTCACC G^GT[ACGT]ACC
BstPI 531 GGTCACC G^GT[ACGT]ACC
EcoO65I 531 GGTCACC G^GT[ACGT]ACC
Hin1I 452 GGCGCC G[GA]^CG[CT]C
HphI 533 TCACC GGTGA(8/7)
KasI 452 GGCGCC G^GCGCC
MaeIII 532 GTCAC ^GT[ACGT]AC
MroI 23 TCCGGA T^CCGGA
MseI 278 TTAA T^TAA
NarI 452 GGCGCC GG^CGCC
PflFI 952 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 313 GACTC GAGTC(4/5)
PpuMI 229 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 667 GAGCTC GAGCT^C
SfoI 452 GGCGCC GGC^GCC
SmaI 391 CCCGGG CCC^GGG
StuI 558 AGGCCT AGG^CCT
TfiI 737 GAATC G^A[AT]TC
Tru9I 278 TTAA T^TAA
Tsp45I 532 GTCAC ^GT[GC]AC
Tsp509I 154 AATT ^AATT
Tth111I 952 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 17 CCACGCTCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 391 CCCGGG C^CCGGG
XmaI 391 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 402 CGCG CG^CG
1002 CGCG
Asp700I 64 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
636 GAACCTCTTC
AvaII 5 GGACC G^G[AT]CC
230 GGTCC
BalI 371 TGGCCA TGG^CCA
842 TGGCCA
BclI 470 TGATCA T^GATCA
803 TGATCA
BsaWI 23 TCCGGA [AT]^CCGG[AT]
773 ACCGGA
BstUI 402 CGCG CG^CG
1002 CGCG
DraII 229 GGGTCCC [GA]G^G[ACGT]CC[CT]
1006 AGGCCCC
DsaI 368 CCGTGG C^C[GA][CT]GG
537 CCACGG
EaeI 371 TGGCCA [CT]^GGCC[GA]
842 TGGCCA
EcoO109I 229 GGGTCCC [GA]G^G[ACGT]CC[CT]
1006 AGGCCCC
FbaI 470 TGATCA T^GATCA
803 TGATCA
HaeII 439 AGCGCC [GA]GCGC^[CT]
452 GGCGCC
HinfI 313 GACTC G^A[ACGT]TC
737 GAATC
MluNI 371 TGGCCA TGG^CCA
842 TGGCCA
MscI 371 TGGCCA TGG^CCA
842 TGGCCA
MvnI 402 CGCG CG^CG
1002 CGCG
MwoI 453 GCGCCTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
723 GCGGAGGATGC
NspI 386 ACATGC [GA]CATG^[CT]
1106 GCATGT
SfaNI 729 GATGC GCATC(5/9)
168 GCATC
TaqI 293 TCGA T^CGA
770 TCGA
TspRI 467 CAGTG CA[GC]TG[ACGT][ACGT]^
1061 CAGTG
TthHB8I 293 TCGA T^CGA
770 TCGA
XmnI 64 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
636 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MslI MspI MvaI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI XhoII