(Circular) (Six-base) MAPSORT of: pFN21ASDA1872.seq Check: 3060 from: 1 to: 5842 pFN21ASDA1872 5842 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2792 2837 3026 3081 3905 5289 5557 1632 Size: 1160 45 189 55 824 1384 268 1917 Fragments arranged by size: 1917 1384 1160 824 268 189 55 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 2959 4741 5718 1203 Size: 1756 1782 977 1327 Fragments arranged by size: 1782 1756 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 2390 1166 Size: 1224 4618 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5842 AlwNI CAG_nnn'CTG Cuts at: 3585 5157 3585 Size: 1572 4270 ApaLI G'TGCA_C Cuts at: 2918 3899 5055 2918 Size: 981 1156 3705 Fragments arranged by size: 3705 1156 981 ApoI r'AATT_y Cuts at: 1493 2176 3151 3377 3529 5638 1493 Size: 683 975 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 683 226 152 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 2414 3167 1870 Size: 73 471 753 4545 Fragments arranged by size: 4545 753 471 73 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2475 2732 3079 3163 4618 Size: 325 217 627 688 257 347 84 1455 Cuts at: 4618 618 Size: 1842 Fragments arranged by size: 1842 1455 688 627 347 325 257 217 84 BanII G_rGCy'C Cuts at: 1391 2694 3161 1391 Size: 1303 467 4072 Fragments arranged by size: 4072 1303 467 BbeI G_GCGC'C Cuts at: 2479 2479 Size: 5842 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2154 2210 2385 2593 2937 928 Size: 971 255 56 175 208 344 3833 Fragments arranged by size: 3833 971 344 255 208 175 56 BbvCI CC'TCA_GC Cuts at: 2703 2703 Size: 5842 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 3734 4944 2272 Size: 1462 1210 3170 Fragments arranged by size: 3170 1462 1210 BclI T'GATC_A Cuts at: 1548 2493 2826 1548 Size: 945 333 4564 Fragments arranged by size: 4564 945 333 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4453 136 Size: 107 122 71 631 605 2781 1525 Fragments arranged by size: 2781 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2089 5837 2089 Size: 3748 2094 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 2922 3903 5059 1790 Size: 1132 981 1156 2573 Fragments arranged by size: 2573 1156 1132 981 BmgBI CAC'GTC Cuts at: 1318 2065 2962 1318 Size: 747 897 4198 Fragments arranged by size: 4198 897 747 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3573 4531 442 Size: 416 53 83 399 24 2156 958 1753 Fragments arranged by size: 2156 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 2353 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2390 2957 4502 1239 Size: 1124 27 567 1545 2579 Fragments arranged by size: 2579 1545 1124 567 27 Bpu10I CC'TnA_GC Cuts at: 2703 2703 Size: 5842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3967 4832 5130 3967 Size: 865 298 4679 Fragments arranged by size: 4679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 2324 3112 4505 882 Size: 617 813 12 788 1393 2219 Fragments arranged by size: 2219 1393 813 788 617 12 BsaAI yAC'GTr Cuts at: 493 1204 2917 3139 3633 493 Size: 711 1713 222 494 2702 Fragments arranged by size: 2702 1713 711 494 222 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2476 3649 4031 4666 Size: 53 83 186 936 943 1173 382 635 Cuts at: 4666 275 Size: 1451 Fragments arranged by size: 1451 1173 943 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2046 2796 4275 4673 4947 5094 5505 2046 Size: 750 1479 398 274 147 411 2383 Fragments arranged by size: 2383 1479 750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2143 2173 1178 Size: 30 165 30 740 30 4847 Fragments arranged by size: 4847 740 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2151 1186 Size: 965 4877 BseYI C'CCAG_C Cuts at: 3033 5045 5541 3033 Size: 2012 496 3334 Fragments arranged by size: 3334 2012 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 1163 Size: 573 774 4495 Fragments arranged by size: 4495 774 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2797 3292 4053 5081 1540 Size: 275 186 796 495 761 1028 2301 Fragments arranged by size: 2301 1028 796 761 495 275 186 BsiHKAI G_wGCw'C Cuts at: 2514 2694 2922 3903 3988 5059 2514 Size: 180 228 981 85 1071 3297 Fragments arranged by size: 3297 1071 981 228 180 85 BsmI GAATG_Cn' Cuts at: 3353 3446 3353 Size: 93 5749 BsmBI CGTCTCn'nnnn_ Cuts at: 2108 2108 Size: 5842 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2514 2694 2922 3161 3903 3988 5059 Size: 399 724 180 228 239 742 85 1071 Cuts at: 5059 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 724 399 239 228 180 85 BspEI T'CCGG_A Cuts at: 2046 5505 2046 Size: 3459 2383 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2532 3197 844 Size: 1688 665 3489 Fragments arranged by size: 3489 1688 665 BsrBI CCG'CTC Cuts at: 1394 1823 3729 1394 Size: 429 1906 3507 Fragments arranged by size: 3507 1906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4337 4511 65 Size: 1183 3089 174 1396 Fragments arranged by size: 3089 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4486 4673 1700 Size: 110 2676 187 2869 Fragments arranged by size: 2869 2676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 2842 3902 4914 2842 Size: 1060 1012 3770 Fragments arranged by size: 3770 1060 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 2007 4646 2007 Size: 2639 3203 BstEII G'GTnAC_C Cuts at: 1184 2554 1184 Size: 1370 4472 BstYI r'GATC_y Cuts at: 1972 2089 2428 2907 2967 3172 3539 3924 3941 Size: 117 339 479 60 205 367 385 17 Cuts at: 3941 5393 5479 5491 5837 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 479 385 367 346 339 205 117 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2391 2560 513 Size: 552 957 369 169 3795 Fragments arranged by size: 3795 957 552 369 169 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5103 Fragments arranged by size: 5103 683 56 BtsI GCAGTG_nn' Cuts at: 3358 4152 4172 3358 Size: 794 20 5028 Fragments arranged by size: 5028 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 3146 3146 Size: 5842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2394 2865 3289 Size: 54 1425 50 275 18 564 471 424 Cuts at: 3289 4178 5807 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 564 471 424 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 2669 2988 3057 3772 1353 Size: 753 563 319 69 715 3423 Fragments arranged by size: 3423 753 715 563 319 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2500 2761 4429 4803 4949 387 Size: 1064 138 911 261 1668 374 146 1280 Fragments arranged by size: 1668 1280 1064 911 374 261 146 138 EcoICRI GAG'CTC Cuts at: 2692 2692 Size: 5842 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2390 2792 2837 2957 3026 3081 Size: 393 731 27 402 45 120 69 55 Cuts at: 3081 3905 4502 5289 5557 1239 Size: 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 731 597 402 393 268 120 69 55 45 27 EcoO109I rG'GnC_Cy Cuts at: 2253 3030 2253 Size: 777 5065 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 2466 2479 4989 1678 Size: 788 13 2510 2531 Fragments arranged by size: 2531 2510 788 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 4483 4515 4557 Size: 32 135 32 569 32 2310 32 42 Cuts at: 4557 4589 1373 Size: 32 2626 Fragments arranged by size: 2626 2310 569 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 3432 669 Size: 988 281 1248 246 3079 Fragments arranged by size: 3079 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KasI G'GCGC_C Cuts at: 2475 2475 Size: 5842 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4956 5140 1305 Size: 3651 184 2007 Fragments arranged by size: 3651 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 2867 10 Size: 54 1425 343 564 471 2985 Fragments arranged by size: 2985 1425 564 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 2549 2717 2951 3800 4159 4318 Size: 648 1224 159 168 234 849 359 159 Cuts at: 4318 518 Size: 2042 Fragments arranged by size: 2042 1224 849 648 359 234 168 159 159 MspA1I CmG'CkG Cuts at: 1606 3258 3935 5083 5328 1606 Size: 1652 677 1148 245 2120 Fragments arranged by size: 2120 1652 1148 677 245 NarI GG'CG_CC Cuts at: 2476 2476 Size: 5842 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4333 Fragments arranged by size: 4333 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 2413 3133 3200 4745 1132 Size: 97 1184 720 67 1545 2229 Fragments arranged by size: 2229 1545 1184 720 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5842 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2253 2253 Size: 5842 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PstI C_TGCA'G Cuts at: 830 3194 830 Size: 2364 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 SacI G_AGCT'C Cuts at: 2694 2694 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SanDI GG'GwC_CC Cuts at: 2253 2253 Size: 5842 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 4090 5802 4090 Size: 1712 4130 SfcI C'TryA_G Cuts at: 826 1045 3190 4325 5006 5197 826 Size: 219 2145 1135 681 191 1471 Fragments arranged by size: 2145 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2477 2477 Size: 5842 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 2416 3169 1872 Size: 544 753 4545 Fragments arranged by size: 4545 753 544 SmlI C'TyrA_G Cuts at: 820 1017 1943 3946 4847 5109 820 Size: 197 926 2003 901 262 1553 Fragments arranged by size: 2003 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3139 3633 493 Size: 2646 494 2702 Fragments arranged by size: 2702 2646 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 2583 1517 Size: 1066 4776 StyI C'CwwG_G Cuts at: 513 1065 2022 3281 513 Size: 552 957 1259 3074 Fragments arranged by size: 3074 1259 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2348 2573 3869 3886 4039 4224 4669 Size: 1255 768 225 1296 17 153 185 445 Cuts at: 4669 325 Size: 1498 Fragments arranged by size: 1498 1296 1255 768 445 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4088 5800 96 Size: 274 80 33 51 1443 2111 1712 138 Fragments arranged by size: 2111 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 5802 5834 2143 Size: 32 3627 32 2151 Fragments arranged by size: 3627 2151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2978 1316 Size: 1662 4180 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2047 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 2414 3167 1870 Size: 544 753 4545 Fragments arranged by size: 4545 753 544 XmnI GAAnn'nnTTC Cuts at: 2091 2663 3971 2091 Size: 572 1308 3962 Fragments arranged by size: 3962 1308 572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI