Restriction map for
pFN21ASDA1872
(Linear) (Six-base) MAPSORT of: KIAA1872.seq Check: 1536 from: 1 to: 1113
KIAA1872.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 814 1003 1058 1113
Size: 769 45 189 55 55
Fragments arranged by size:
769 189 55 55 45
AflIII A'CryG_T
Cuts at: 0 936 1113
Size: 936 177
AleI CACnn'nnGTG
Cuts at: 0 367 1113
Size: 367 746
ApaLI G'TGCA_C
Cuts at: 0 895 1113
Size: 895 218
ApoI r'AATT_y
Cuts at: 0 153 1113
Size: 153 960
AvaI C'yCGr_G
Cuts at: 0 391 1113
Size: 391 722
BanI G'GyrC_C
Cuts at: 0 452 709 1056 1113
Size: 452 257 347 57
Fragments arranged by size:
452 347 257 57
BanII G_rGCy'C
Cuts at: 0 671 1113
Size: 671 442
BbeI G_GCGC'C
Cuts at: 0 456 1113
Size: 456 657
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 187 362 570 914 1113
Size: 131 56 175 208 344 199
Fragments arranged by size:
344 208 199 175 131 56
BbvCI CC'TCA_GC
Cuts at: 0 680 1113
Size: 680 433
BciVI GTATCCnnnnn_n'
Cuts at: 0 249 1113
Size: 249 864
BclI T'GATC_A
Cuts at: 0 470 803 1113
Size: 470 333 310
Fragments arranged by size:
470 333 310
BglII A'GATC_T
Cuts at: 0 66 1113
Size: 66 1047
Bme1580I G_kGCm'C
Cuts at: 0 899 1113
Size: 899 214
BmgBI CAC'GTC
Cuts at: 0 42 939 1113
Size: 42 897 174
Fragments arranged by size:
897 174 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 330 1113
Size: 330 783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 367 934 1113
Size: 340 27 567 179
Fragments arranged by size:
567 340 179 27
Bpu10I CC'TnA_GC
Cuts at: 0 680 1113
Size: 680 433
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 301 1089 1113
Size: 289 12 788 24
Fragments arranged by size:
788 289 24 12
BsaAI yAC'GTr
Cuts at: 0 894 1113
Size: 894 219
BsaHI Gr'CG_yC
Cuts at: 0 453 1113
Size: 453 660
BsaWI w'CCGG_w
Cuts at: 0 23 773 1113
Size: 23 750 340
Fragments arranged by size:
750 340 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 120 150 1113
Size: 120 30 963
Fragments arranged by size:
963 120 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 128 1113
Size: 128 985
BseYI C'CCAG_C
Cuts at: 0 1010 1113
Size: 1010 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1113
Size: 487 626
BsiEI CG_ry'CG
Cuts at: 0 774 1113
Size: 774 339
BsiHKAI G_wGCw'C
Cuts at: 0 491 671 899 1113
Size: 491 180 228 214
Fragments arranged by size:
491 228 214 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 1113
Size: 85 1028
Bsp1286I G_dGCh'C
Cuts at: 0 491 671 899 1113
Size: 491 180 228 214
Fragments arranged by size:
491 228 214 180
BspEI T'CCGG_A
Cuts at: 0 23 1113
Size: 23 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 509 1113
Size: 509 604
BssSI C'ACGA_G
Cuts at: 0 819 1113
Size: 819 294
BstEII G'GTnAC_C
Cuts at: 0 531 1113
Size: 531 582
BstYI r'GATC_y
Cuts at: 0 66 405 884 944 1113
Size: 66 339 479 60 169
Fragments arranged by size:
479 339 169 66 60
BtgI C'CryG_G
Cuts at: 0 368 537 1113
Size: 368 169 576
Fragments arranged by size:
576 368 169
EaeI y'GGCC_r
Cuts at: 0 371 842 1113
Size: 371 471 271
Fragments arranged by size:
471 371 271
EarI CTCTTCn'nnn_
Cuts at: 0 83 646 965 1034 1113
Size: 83 563 319 69 79
Fragments arranged by size:
563 319 83 79 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 477 738 1113
Size: 477 261 375
Fragments arranged by size:
477 375 261
EcoICRI GAG'CTC
Cuts at: 0 669 1113
Size: 669 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 367 769 814 934 1003 1058 1113
Size: 340 27 402 45 120 69 55 55
Fragments arranged by size:
402 340 120 69 55 55 45 27
EcoO109I rG'GnC_Cy
Cuts at: 0 230 1007 1113
Size: 230 777 106
Fragments arranged by size:
777 230 106
HaeII r_GCGC'y
Cuts at: 0 443 456 1113
Size: 443 13 657
Fragments arranged by size:
657 443 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 1113
Size: 118 32 963
Fragments arranged by size:
963 118 32
KasI G'GCGC_C
Cuts at: 0 452 1113
Size: 452 661
MscI TGG'CCA
Cuts at: 0 373 844 1113
Size: 373 471 269
Fragments arranged by size:
471 373 269
MslI CAynn'nnrTG
Cuts at: 0 367 526 694 928 1113
Size: 367 159 168 234 185
Fragments arranged by size:
367 234 185 168 159
NarI GG'CG_CC
Cuts at: 0 453 1113
Size: 453 660
NspI r_CATG'y
Cuts at: 0 390 1110 1113
Size: 390 720 3
Fragments arranged by size:
720 390 3
PpuMI rG'GwC_Cy
Cuts at: 0 230 1113
Size: 230 883
SacI G_AGCT'C
Cuts at: 0 671 1113
Size: 671 442
SanDI GG'GwC_CC
Cuts at: 0 230 1113
Size: 230 883
SfoI GGC'GCC
Cuts at: 0 454 1113
Size: 454 659
SmaI CCC'GGG
Cuts at: 0 393 1113
Size: 393 720
StuI AGG'CCT
Cuts at: 0 560 1113
Size: 560 553
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 550 1113
Size: 325 225 563
Fragments arranged by size:
563 325 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 120 152 1113
Size: 120 32 961
Fragments arranged by size:
961 120 32
Tth111I GACn'n_nGTC
Cuts at: 0 955 1113
Size: 955 158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 1113
Size: 24 1089
XmaI C'CCGG_G
Cuts at: 0 391 1113
Size: 391 722
XmnI GAAnn'nnTTC
Cuts at: 0 68 640 1113
Size: 68 572 473
Fragments arranged by size:
572 473 68
Enzymes that do cut:
AcuI AflIII AleI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII Bme1580I BmgBI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BssSI BstEII BstYI BtgI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI
NarI NspI PpuMI SacI SanDI SfoI SmaI StuI
TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BfrBI BglI BlpI BmrI
BmtI BpuEI BsaBI BsiWI BsmI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI TatI XbaI XhoI ZraI