(Circular) (Six-base) MAPSORT of: pFN21ASDA1860.seq Check: 8084 from: 1 to: 6793 pFN21ASDA1860 6793 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4603 278 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4136 1937 Size: 2199 4594 Acc65I G'GTAC_C Cuts at: 4114 4114 Size: 6793 AclI AA'CG_TT Cuts at: 1563 4920 5293 1563 Size: 3357 373 3063 Fragments arranged by size: 3357 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 2611 3383 4856 6240 6508 1632 Size: 956 23 772 1473 1384 268 1917 Fragments arranged by size: 1917 1473 1384 956 772 268 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6596 AflIII A'CryG_T Cuts at: 1203 2661 2856 5692 6669 1203 Size: 1458 195 2836 977 1327 Fragments arranged by size: 2836 1458 1327 977 195 AgeI A'CCGG_T Cuts at: 5624 5624 Size: 6793 AhdI GACnn_n'nnGTC Cuts at: 4015 5522 4015 Size: 1507 5286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6793 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6793 AlwNI CAG_nnn'CTG Cuts at: 2329 4536 6108 2329 Size: 2207 1572 3014 Fragments arranged by size: 3014 2207 1572 ApaI G_GGCC'C Cuts at: 3795 3795 Size: 6793 ApaLI G'TGCA_C Cuts at: 2528 4850 6006 2528 Size: 2322 1156 3315 Fragments arranged by size: 3315 2322 1156 ApoI r'AATT_y Cuts at: 1493 4102 4328 4480 6589 1493 Size: 2609 226 152 2109 1697 Fragments arranged by size: 2609 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5347 160 Size: 872 4315 1606 Fragments arranged by size: 4315 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2171 2496 2924 2996 3574 4118 Size: 73 104 124 325 428 72 578 544 Cuts at: 4118 1870 Size: 4545 Fragments arranged by size: 4545 578 544 428 325 124 104 73 72 BamHI G'GATC_C Cuts at: 3968 4123 4490 3968 Size: 155 367 6271 Fragments arranged by size: 6271 367 155 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2138 3155 3187 3227 Size: 325 217 627 291 60 1017 32 40 Cuts at: 3227 3626 3637 3670 3950 4114 5569 618 Size: 399 11 33 280 164 1455 1842 Fragments arranged by size: 1842 1455 1017 627 399 325 291 280 217 164 60 40 33 32 11 BanII G_rGCy'C Cuts at: 1391 2678 2997 3795 4112 1391 Size: 1287 319 798 317 4072 Fragments arranged by size: 4072 1287 798 319 317 BbeI G_GCGC'C Cuts at: 3159 3191 3954 3159 Size: 32 763 5998 Fragments arranged by size: 5998 763 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 2414 3137 928 Size: 971 120 395 723 4584 Fragments arranged by size: 4584 971 723 395 120 BbvCI CC'TCA_GC Cuts at: 2207 2207 Size: 6793 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4984 5018 1903 Size: 34 3047 34 3678 Fragments arranged by size: 3678 3047 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4685 5895 4685 Size: 1210 5583 BclI T'GATC_A Cuts at: 1548 1548 Size: 6793 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3821 3841 5404 Size: 107 122 71 631 605 2149 20 1563 Cuts at: 5404 136 Size: 1525 Fragments arranged by size: 2149 1563 1525 631 605 122 107 71 20 BglII A'GATC_T Cuts at: 6788 6788 Size: 6793 BlpI GC'TnA_GC Cuts at: 3582 4210 3582 Size: 628 6165 Bme1580I G_kGCm'C Cuts at: 1790 2143 2532 2799 3642 3795 4854 6010 1790 Size: 353 389 267 843 153 1059 1156 2573 Fragments arranged by size: 2573 1156 1059 843 389 353 267 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6793 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2144 4524 5482 Size: 416 53 83 399 24 727 2380 958 Cuts at: 5482 442 Size: 1753 Fragments arranged by size: 2380 1753 958 727 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6793 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2463 2761 3421 3640 4101 5453 1239 Size: 1224 298 660 219 461 1352 2579 Fragments arranged by size: 2579 1352 1224 660 461 298 219 Bpu10I CC'TnA_GC Cuts at: 2207 3622 2207 Size: 1415 5378 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 4918 5783 6081 2789 Size: 2129 865 298 3501 Fragments arranged by size: 3501 2129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5456 882 Size: 617 3957 2219 Fragments arranged by size: 3957 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2188 4090 4584 493 Size: 711 984 1902 494 2702 Fragments arranged by size: 2702 1902 984 711 494 BsaBI GATnn'nnATC Cuts at: 2954 6787 2954 Size: 3833 2960 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3156 3188 3951 4600 Size: 53 83 186 936 1623 32 763 649 Cuts at: 4600 4982 5617 275 Size: 382 635 1451 Fragments arranged by size: 1623 1451 936 763 649 635 382 186 83 53 32 BsaWI w'CCGG_w Cuts at: 5226 5624 5898 6045 6456 5226 Size: 398 274 147 411 5563 Fragments arranged by size: 5563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3403 3433 4074 4104 1178 Size: 30 165 30 2000 30 641 30 3867 Fragments arranged by size: 3867 2000 641 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2089 2189 2987 3014 3152 3383 1186 Size: 903 100 798 27 138 231 4596 Fragments arranged by size: 4596 903 798 231 138 100 27 BseYI C'CCAG_C Cuts at: 2301 2312 2644 2905 3121 3191 5996 6492 2301 Size: 11 332 261 216 70 2805 496 2602 Fragments arranged by size: 2805 2602 496 332 261 216 70 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3297 3305 3803 3810 1163 Size: 573 1561 8 498 7 4146 Fragments arranged by size: 4146 1561 573 498 8 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2059 4243 5004 6032 1540 Size: 275 186 58 2184 761 1028 2301 Fragments arranged by size: 2301 2184 1028 761 275 186 58 BsiHKAI G_wGCw'C Cuts at: 2532 3347 3512 4854 4939 6010 2532 Size: 815 165 1342 85 1071 3315 Fragments arranged by size: 3315 1342 1071 815 165 85 BsmI GAATG_Cn' Cuts at: 4304 4397 4304 Size: 93 6700 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2532 2678 2799 2997 3347 3512 Size: 399 353 389 146 121 198 350 165 Cuts at: 3512 3642 3795 4112 4854 4939 6010 1391 Size: 130 153 317 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 389 353 350 317 198 165 153 146 130 121 85 BspEI T'CCGG_A Cuts at: 6456 6456 Size: 6793 BspHI T'CATG_A Cuts at: 2349 2934 4682 6412 2349 Size: 585 1748 1730 2730 Fragments arranged by size: 2730 1748 1730 585 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4148 844 Size: 3304 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2097 2130 3373 3490 3688 4680 1394 Size: 429 274 33 1243 117 198 992 3507 Fragments arranged by size: 3507 1243 992 429 274 198 117 33 BsrDI GCAATG_nn' Cuts at: 65 1248 5288 5462 65 Size: 1183 4040 174 1396 Fragments arranged by size: 4040 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3750 5437 5624 1700 Size: 110 1940 1687 187 2869 Fragments arranged by size: 2869 1940 1687 187 110 BsrGI T'GTAC_A Cuts at: 96 2554 2760 96 Size: 2458 206 4129 Fragments arranged by size: 4129 2458 206 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6793 BssSI C'ACGA_G Cuts at: 2462 3505 4853 5865 2462 Size: 1043 1348 1012 3390 Fragments arranged by size: 3390 1348 1043 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6793 BstBI TT'CG_AA Cuts at: 2007 5597 2007 Size: 3590 3203 BstEII G'GTnAC_C Cuts at: 1184 3901 1184 Size: 2717 4076 BstXI CCAn_nnnn'nTGG Cuts at: 3445 3445 Size: 6793 BstYI r'GATC_y Cuts at: 1972 3480 3968 4123 4490 4875 4892 6344 6430 Size: 1508 488 155 367 385 17 1452 86 Cuts at: 6430 6442 6788 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1508 1452 488 385 367 346 155 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4013 4013 Size: 6793 BtgI C'CryG_G Cuts at: 513 1065 3405 3444 3632 3730 513 Size: 552 2340 39 188 98 3576 Fragments arranged by size: 3576 2340 552 188 98 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2143 487 Size: 683 56 917 5137 Fragments arranged by size: 5137 917 683 56 BtsI GCAGTG_nn' Cuts at: 2095 4309 5103 5123 2095 Size: 2214 794 20 3765 Fragments arranged by size: 3765 2214 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6793 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6758 DraI TTT'AAA Cuts at: 4097 4097 Size: 6793 DraIII CAC_nnn'GTG Cuts at: 2138 2138 Size: 6793 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5800 809 Size: 507 4484 1802 Fragments arranged by size: 4484 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2473 3736 Size: 54 1425 50 275 18 262 381 1263 Cuts at: 3736 4240 5129 6758 8 Size: 504 889 1629 43 Fragments arranged by size: 1629 1425 1263 889 504 381 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4240 1537 Size: 275 2428 4090 Fragments arranged by size: 4090 2428 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3265 3872 4723 1353 Size: 1912 607 851 3423 Fragments arranged by size: 3423 1912 851 607 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3371 3508 5380 5754 5900 387 Size: 1064 138 1782 137 1872 374 146 1280 Fragments arranged by size: 1872 1782 1280 1064 374 146 138 137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 2588 2611 2761 3383 3421 3640 Size: 393 831 125 23 150 622 38 219 Cuts at: 3640 4101 4856 5453 6240 6508 1239 Size: 461 755 597 787 268 1524 Fragments arranged by size: 1524 831 787 755 622 597 461 393 268 219 150 125 38 23 EcoO109I rG'GnC_Cy Cuts at: 2356 2563 2845 3792 4009 2356 Size: 207 282 947 217 5140 Fragments arranged by size: 5140 947 282 217 207 EcoRI G'AATT_C Cuts at: 4102 4480 4102 Size: 378 6415 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2245 2245 Size: 6793 FspI TGC'GCA Cuts at: 5299 5299 Size: 6793 HaeII r_GCGC'y Cuts at: 1678 3159 3191 3459 3589 3954 5940 1678 Size: 1481 32 268 130 365 1986 2531 Fragments arranged by size: 2531 1986 1481 365 268 130 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 3403 3435 3575 Size: 32 135 32 476 32 1323 32 140 Cuts at: 3575 3607 3960 3992 4001 4033 5434 5466 5508 Size: 32 353 32 9 32 1401 32 42 Cuts at: 5508 5540 1373 Size: 32 2626 Fragments arranged by size: 2626 1401 1323 476 353 140 135 42 32 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 2862 4137 4383 669 Size: 988 281 924 1275 246 3079 Fragments arranged by size: 3079 1275 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6793 HpaI GTT'AAC Cuts at: 4383 4383 Size: 6793 KasI G'GCGC_C Cuts at: 3155 3187 3950 3155 Size: 32 763 5998 Fragments arranged by size: 5998 763 32 KpnI G_GTAC'C Cuts at: 4118 4118 Size: 6793 MfeI C'AATT_G Cuts at: 4392 4392 Size: 6793 MluI A'CGCG_T Cuts at: 6669 6669 Size: 6793 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2533 5907 6091 1305 Size: 1228 3374 184 2007 Fragments arranged by size: 3374 2007 1228 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3738 10 Size: 54 1425 343 1906 3065 Fragments arranged by size: 3065 1906 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4751 5110 5269 518 Size: 648 3585 359 159 2042 Fragments arranged by size: 3585 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2305 2775 3250 3407 3939 4209 4886 6034 Size: 699 470 475 157 532 270 677 1148 Cuts at: 6034 6279 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 699 677 532 475 470 270 245 157 NarI GG'CG_CC Cuts at: 3156 3188 3951 3156 Size: 32 763 5998 Fragments arranged by size: 5998 763 32 NcoI C'CATG_G Cuts at: 513 1065 3444 3730 513 Size: 552 2379 286 3576 Fragments arranged by size: 3576 2379 552 286 NdeI CA'TA_TG Cuts at: 387 2683 387 Size: 2296 4497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6793 NotI GC'GGCC_GC Cuts at: 4240 4240 Size: 6793 NspI r_CATG'y Cuts at: 1132 1229 2860 4151 5696 1132 Size: 97 1631 1291 1545 2229 Fragments arranged by size: 2229 1631 1545 1291 97 PasI CC'CwG_GG Cuts at: 1348 3161 3964 1348 Size: 1813 803 4177 Fragments arranged by size: 4177 1813 803 PciI A'CATG_T Cuts at: 2856 5692 2856 Size: 2836 3957 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6793 PfoI T'CCnGG_A Cuts at: 3744 3744 Size: 6793 PmeI GTTT'AAAC Cuts at: 4097 4097 Size: 6793 PmlI CAC'GTG Cuts at: 2188 2188 Size: 6793 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4862 4894 6375 6407 4862 Size: 32 1481 32 5248 Fragments arranged by size: 5248 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2563 2845 4009 2563 Size: 282 1164 5347 Fragments arranged by size: 5347 1164 282 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6793 PsiI TTA'TAA Cuts at: 4363 4363 Size: 6793 PspOMI G'GGCC_C Cuts at: 3791 3791 Size: 6793 PspXI vC'TCGA_Gb Cuts at: 2496 2496 Size: 6793 PstI C_TGCA'G Cuts at: 830 2325 3401 4145 830 Size: 1495 1076 744 3478 Fragments arranged by size: 3478 1495 1076 744 PvuI CG_AT'CG Cuts at: 2001 2059 2001 Size: 58 6735 PvuII CAG'CTG Cuts at: 2305 2305 Size: 6793 SacII CC_GC'GG Cuts at: 3408 3408 Size: 6793 SalI G'TCGA_C Cuts at: 1936 4135 1936 Size: 2199 4594 SanDI GG'GwC_CC Cuts at: 2845 2845 Size: 6793 SbfI CC_TGCA'GG Cuts at: 4145 4145 Size: 6793 ScaI AGT'ACT Cuts at: 2826 5041 6753 2826 Size: 2215 1712 2866 Fragments arranged by size: 2866 2215 1712 SexAI A'CCwGG_T Cuts at: 2418 2418 Size: 6793 SfcI C'TryA_G Cuts at: 826 1045 2321 3397 4141 5276 5957 6148 826 Size: 219 1276 1076 744 1135 681 191 1471 Fragments arranged by size: 1471 1276 1135 1076 744 681 219 191 SfoI GGC'GCC Cuts at: 3157 3189 3952 3157 Size: 32 763 5998 Fragments arranged by size: 5998 763 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6793 SmaI CCC'GGG Cuts at: 1872 2049 3576 4120 1872 Size: 177 1527 544 4545 Fragments arranged by size: 4545 1527 544 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 2496 2804 2924 4897 5798 6060 Size: 197 926 553 308 120 1973 901 262 Cuts at: 6060 820 Size: 1553 Fragments arranged by size: 1973 1553 926 901 553 308 262 197 120 SnaBI TAC'GTA Cuts at: 493 4090 4584 493 Size: 3597 494 2702 Fragments arranged by size: 3597 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6793 SphI G_CATG'C Cuts at: 1132 4151 1132 Size: 3019 3774 SspI AAT'ATT Cuts at: 5 52 2551 4717 5 Size: 47 2499 2166 2081 Fragments arranged by size: 2499 2166 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6793 StyI C'CwwG_G Cuts at: 513 1065 2082 3444 3730 4036 4232 513 Size: 552 1017 1362 286 306 196 3074 Fragments arranged by size: 3074 1362 1017 552 306 286 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 4820 4837 4990 5175 5620 325 Size: 1255 546 2694 17 153 185 445 1498 Fragments arranged by size: 2694 1498 1255 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2554 2760 2824 Size: 274 80 33 51 1443 577 206 64 Cuts at: 2824 2918 3090 5039 6751 96 Size: 94 172 1949 1712 138 Fragments arranged by size: 1949 1712 1443 577 274 206 172 138 94 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6753 6785 6753 Size: 32 6761 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6793 XbaI T'CTAG_A Cuts at: 4129 4129 Size: 6793 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2242 3664 2131 Size: 111 1422 5260 Fragments arranged by size: 5260 1422 111 XhoI C'TCGA_G Cuts at: 1943 2496 2924 1943 Size: 553 428 5812 Fragments arranged by size: 5812 553 428 XmaI C'CCGG_G Cuts at: 1870 2047 3574 4118 1870 Size: 177 1527 544 4545 Fragments arranged by size: 4545 1527 544 177 XmnI GAAnn'nnTTC Cuts at: 2330 3214 4922 2330 Size: 884 1708 4201 Fragments arranged by size: 4201 1708 884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4601 276 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacI SapI SfiI SrfI SwaI