Restriction map for
pFN21ASDA1857
(Circular) (Six-base) MAPSORT of: pFN21ASDA1857.seq Check: 32 from: 1 to: 6319
pFN21ASDA1857 6319 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2868 3434 2868
Size: 566 5753
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 4382 5766 6034 1632
Size: 548 2202 1384 268 1917
Fragments arranged by size:
2202 1917 1384 548 268
AfeI AGC'GCT
Cuts at: 3443 3443
Size: 6319
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 2201 2574 3384 5218 6195 1203
Size: 998 373 810 1834 977 1327
Fragments arranged by size:
1834 1327 998 977 810 373
AgeI A'CCGG_T
Cuts at: 3279 5150 3279
Size: 1871 4448
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 2816 3470 1166
Size: 1650 654 4015
Fragments arranged by size:
4015 1650 654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2804 2834 3446 1184
Size: 1620 30 612 4057
Fragments arranged by size:
4057 1620 612 30
AlwNI CAG_nnn'CTG
Cuts at: 3406 4062 5634 3406
Size: 656 1572 4091
Fragments arranged by size:
4091 1572 656
ApaI G_GGCC'C
Cuts at: 2117 2569 3329 2117
Size: 452 760 5107
Fragments arranged by size:
5107 760 452
ApaLI G'TGCA_C
Cuts at: 3272 4376 5532 3272
Size: 1104 1156 4059
Fragments arranged by size:
4059 1156 1104
ApoI r'AATT_y
Cuts at: 1493 2986 3628 3854 4006 6115 1493
Size: 1493 642 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 3539 3644 1870
Size: 73 1596 105 4545
Fragments arranged by size:
4545 1596 105 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2810 3363 3422 3640 5095
Size: 325 217 627 1023 553 59 218 1455
Cuts at: 5095 618
Size: 1842
Fragments arranged by size:
1842 1455 1023 627 553 325 218 217
59
BanII G_rGCy'C
Cuts at: 1391 2117 2190 2569 3329 3638 1391
Size: 726 73 379 760 309 4072
Fragments arranged by size:
4072 760 726 379 309 73
BbeI G_GCGC'C
Cuts at: 3367 3367
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5348
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2564 3192 3315
Size: 107 122 71 631 605 892 628 123
Cuts at: 3315 3459 4930 136
Size: 144 1471 1525
Fragments arranged by size:
1525 1471 892 631 628 605 144 123
122 107 71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 2117 2569 3276 3329 4380 5536 1790
Size: 327 452 707 53 1051 1156 2573
Fragments arranged by size:
2573 1156 1051 707 452 327 53
BmgBI CAC'GTC
Cuts at: 1318 2431 1318
Size: 1113 5206
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4050 5008 442
Size: 416 53 83 399 24 2633 958 1753
Fragments arranged by size:
2633 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2377 2495 2569 2753 4979 1239
Size: 1138 118 74 184 2226 2579
Fragments arranged by size:
2579 2226 1138 184 118 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2735 4444 5309 5607 2735
Size: 1709 865 298 3447
Fragments arranged by size:
3447 1709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2202 3284 4982 882
Size: 617 703 1082 1698 2219
Fragments arranged by size:
2219 1698 1082 703 617
BsaAI yAC'GTr
Cuts at: 493 1204 2653 3616 4110 493
Size: 711 1449 963 494 2702
Fragments arranged by size:
2702 1449 963 711 494
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 3364 4126 4508
Size: 53 83 186 936 735 1096 762 382
Cuts at: 4508 5143 275
Size: 635 1451
Fragments arranged by size:
1451 1096 936 762 735 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3279 4752 5150 5424 5571 5982 3279
Size: 1473 398 274 147 411 3616
Fragments arranged by size:
3616 1473 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2840 2870 2894 2924 3080
Size: 30 165 30 1437 30 24 30 156
Cuts at: 3080 3110 1178
Size: 30 4387
Fragments arranged by size:
4387 1437 165 156 30 30 30 30
30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2336 2627 1186
Size: 1147 3 291 4878
Fragments arranged by size:
4878 1147 291 3
BseYI C'CCAG_C
Cuts at: 2369 2625 2803 3190 3586 5522 6018 2369
Size: 256 178 387 396 1936 496 2670
Fragments arranged by size:
2670 1936 496 396 387 256 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2590 2840 1163
Size: 573 297 557 250 4642
Fragments arranged by size:
4642 573 557 297 250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2455 3280 3532 3769 4530 5558
Size: 275 186 454 825 252 237 761 1028
Cuts at: 5558 1540
Size: 2301
Fragments arranged by size:
2301 1028 825 761 454 275 252 237
186
BsiHKAI G_wGCw'C
Cuts at: 2604 2912 2949 3276 4380 4465 5536 2604
Size: 308 37 327 1104 85 1071 3387
Fragments arranged by size:
3387 1104 1071 327 308 85 37
BsmI GAATG_Cn'
Cuts at: 3089 3830 3923 3089
Size: 741 93 5485
Fragments arranged by size:
5485 741 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2190 2569 2604 2912 2949 3276
Size: 399 327 73 379 35 308 37 327
Cuts at: 3276 3329 3638 4380 4465 5536 1391
Size: 53 309 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 327 327 309
308 85 73 53 37 35
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2709 2784 2868 2901 3030 3053 3146 3434
Size: 1865 75 84 33 129 23 93 288
Cuts at: 3434 3674 844
Size: 240 3489
Fragments arranged by size:
3489 1865 288 240 129 93 84 75
33 23
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2454 2690 2957 3011 3186 3279 3293
Size: 110 644 236 267 54 175 93 14
Cuts at: 3293 3467 4963 5150 1700
Size: 174 1496 187 2869
Fragments arranged by size:
2869 1496 644 267 236 187 175 174
110 93 54 14
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 2589 3534 1925
Size: 664 945 4710
Fragments arranged by size:
4710 945 664
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2599 794
Size: 1805 4514
BstBI TT'CG_AA
Cuts at: 2007 5123 2007
Size: 3116 3203
BstEII G'GTnAC_C
Cuts at: 1184 2098 3133 1184
Size: 914 1035 4370
Fragments arranged by size:
4370 1035 914
BstXI CCAn_nnnn'nTGG
Cuts at: 2377 2467 2377
Size: 90 6229
BstYI r'GATC_y
Cuts at: 1972 3649 4016 4401 4418 5870 5956 5968 6314
Size: 1677 367 385 17 1452 86 12 346
Cuts at: 6314 1972
Size: 1977
Fragments arranged by size:
1977 1677 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2191 2191
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2412 2460 3034 513
Size: 552 1347 48 574 3798
Fragments arranged by size:
3798 1347 574 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2050 2939 3172 3181 3835 4629 4649 2050
Size: 889 233 9 654 794 20 3720
Fragments arranged by size:
3720 889 794 654 233 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 2599 2599
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 2452 3594
Size: 54 1425 50 275 18 501 121 1142
Cuts at: 3594 3766 4655 6284 8
Size: 172 889 1629 43
Fragments arranged by size:
1629 1425 1142 889 501 275 172 121
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2452 3766 1537
Size: 275 640 1314 4090
Fragments arranged by size:
4090 1314 640 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 4249 1353
Size: 695 2201 3423
Fragments arranged by size:
3423 2201 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2545 4906 5280 5426 387
Size: 1064 138 956 2361 374 146 1280
Fragments arranged by size:
2361 1280 1064 956 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2377 2495 2569 2753 4382 4979
Size: 393 548 197 118 74 184 1629 597
Cuts at: 4979 5766 6034 1239
Size: 787 268 1524
Fragments arranged by size:
1629 1524 787 597 548 393 268 197
184 118 74
EcoO109I rG'GnC_Cy
Cuts at: 2113 2114 2326 2746 3326 2113
Size: 1 212 420 580 5106
Fragments arranged by size:
5106 580 420 212 1
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 6319
FseI GG_CCGG'CC
Cuts at: 2458 2458
Size: 6319
FspI TGC'GCA
Cuts at: 2705 2780 4825 2705
Size: 75 2045 4199
Fragments arranged by size:
4199 2045 75
HaeII r_GCGC'y
Cuts at: 1678 2042 2742 2804 3367 3445 5466 1678
Size: 364 700 62 563 78 2021 2531
Fragments arranged by size:
2531 2021 700 563 364 78 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 4960 4992 5034
Size: 32 135 32 992 32 2364 32 42
Cuts at: 5034 5066 1373
Size: 32 2626
Fragments arranged by size:
2626 2364 992 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 3363 3363
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 2883 3123 5433 5617 1305
Size: 993 585 240 2310 184 2007
Fragments arranged by size:
2310 2007 993 585 240 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 10
Size: 54 1425 343 501 3996
Fragments arranged by size:
3996 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2816 3470 4277 4636 4795 518
Size: 648 1299 351 654 807 359 159 2042
Fragments arranged by size:
2042 1299 807 654 648 359 351 159
MspA1I CmG'CkG
Cuts at: 1606 2377 2824 3028 3059 3105 3147 3403 3492
Size: 771 447 204 31 46 42 256 89
Cuts at: 3492 3735 4412 5560 5805 1606
Size: 243 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 677 447 256 245 243
204 89 46 42 31
NaeI GCC'GGC
Cuts at: 2456 2692 3013 3188 2456
Size: 236 321 175 5587
Fragments arranged by size:
5587 321 236 175
NarI GG'CG_CC
Cuts at: 3364 3364
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 3034 513
Size: 552 1969 3798
Fragments arranged by size:
3798 1969 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2454 2690 3011 3186 2454
Size: 236 321 175 5587
Fragments arranged by size:
5587 321 236 175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2205 2578 2785 2900 3677 5222 1132
Size: 97 976 373 207 115 777 1545 2229
Fragments arranged by size:
2229 1545 976 777 373 207 115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 2201 2574 5218 2201
Size: 373 2644 3302
Fragments arranged by size:
3302 2644 373
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2340 1108
Size: 1232 5087
PfoI T'CCnGG_A
Cuts at: 2472 2472
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 2653 2653
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3140 3516 1613
Size: 1527 376 4416
Fragments arranged by size:
4416 1527 376
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2113 2565 3325 2113
Size: 452 760 5107
Fragments arranged by size:
5107 760 452
PstI C_TGCA'G
Cuts at: 830 2934 3064 3110 3435 3671 830
Size: 2104 130 46 325 236 3478
Fragments arranged by size:
3478 2104 325 236 130 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 3147 3403 3147
Size: 256 6063
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SapI GCTCTTCn'nnn_
Cuts at: 2048 2048
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 2618 2843 4567 6279 2618
Size: 225 1724 1712 2658
Fragments arranged by size:
2658 1724 1712 225
SfcI C'TryA_G
Cuts at: 826 1045 2930 3060 3106 3431 3667 4802 5483
Size: 219 1885 130 46 325 236 1135 681
Cuts at: 5483 5674 826
Size: 191 1471
Fragments arranged by size:
1885 1471 1135 681 325 236 219 191
130 46
SfoI GGC'GCC
Cuts at: 3365 3365
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 3467 1700
Size: 1767 4552
SmaI CCC'GGG
Cuts at: 1872 3541 3646 1872
Size: 1669 105 4545
Fragments arranged by size:
4545 1669 105
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2750 4423 5324 5586 820
Size: 197 926 807 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 807 262 197
SnaBI TAC'GTA
Cuts at: 493 3616 4110 493
Size: 3123 494 2702
Fragments arranged by size:
3123 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 2785 2900 3677 1132
Size: 1653 115 777 3774
Fragments arranged by size:
3774 1653 777 115
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 2543 1517
Size: 1026 5293
StyI C'CwwG_G
Cuts at: 513 1065 2145 2742 3034 3758 513
Size: 552 1080 597 292 724 3074
Fragments arranged by size:
3074 1080 724 597 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2086 2438 4346 4363 4516 4701 5146
Size: 1255 506 352 1908 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
1908 1498 1255 506 445 352 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 2841 4565
Size: 274 80 33 51 1443 639 225 1724
Cuts at: 4565 6277 96
Size: 1712 138
Fragments arranged by size:
1724 1712 1443 639 274 225 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 2168 1316
Size: 852 5467
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2281
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3539 3644 1870
Size: 1669 105 4545
Fragments arranged by size:
4545 1669 105
XmnI GAAnn'nnTTC
Cuts at: 2987 4448 2987
Size: 1461 4858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FspAI NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI