(Circular) (Six-base) MAPSORT of: pFN21ASDA1848.seq Check: 6142 from: 1 to: 5941 pFN21ASDA1848 5941 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2790 2790 Size: 5941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3141 3284 1937 Size: 1204 143 4594 Fragments arranged by size: 4594 1204 143 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 4004 5388 5656 1632 Size: 938 1434 1384 268 1917 Fragments arranged by size: 1917 1434 1384 938 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 2582 4840 5817 1203 Size: 1379 2258 977 1327 Fragments arranged by size: 2258 1379 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 2771 2903 1166 Size: 1605 132 4204 Fragments arranged by size: 4204 1605 132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 2384 3684 5256 2384 Size: 1300 1572 3069 Fragments arranged by size: 3069 1572 1300 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2099 3250 3476 3628 5737 1493 Size: 606 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 606 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2154 2232 3069 3266 1870 Size: 73 130 81 78 837 197 4545 Fragments arranged by size: 4545 837 197 130 81 78 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2599 2882 2897 2909 2980 Size: 325 217 627 812 283 15 12 71 Cuts at: 2980 3262 4717 618 Size: 282 1455 1842 Fragments arranged by size: 1842 1455 812 627 325 283 282 217 71 15 12 BanII G_rGCy'C Cuts at: 1391 2697 2922 3132 3260 1391 Size: 1306 225 210 128 4072 Fragments arranged by size: 4072 1306 225 210 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2123 2366 2486 928 Size: 971 224 243 120 4383 Fragments arranged by size: 4383 971 243 224 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 2367 1548 Size: 819 5122 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2999 3119 3153 Size: 107 122 71 631 605 1327 120 34 Cuts at: 3153 4552 136 Size: 1399 1525 Fragments arranged by size: 1525 1399 1327 631 605 122 120 107 71 34 BglII A'GATC_T Cuts at: 2187 5936 2187 Size: 3749 2192 BlpI GC'TnA_GC Cuts at: 3230 3358 3230 Size: 128 5813 Bme1580I G_kGCm'C Cuts at: 1790 2233 2912 2985 4002 5158 1790 Size: 443 679 73 1017 1156 2573 Fragments arranged by size: 2573 1156 1017 679 443 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2220 2405 3672 Size: 416 53 83 399 24 803 185 1267 Cuts at: 3672 4630 442 Size: 958 1753 Fragments arranged by size: 1753 1267 958 803 416 399 185 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2486 2771 4601 1239 Size: 1247 285 1830 2579 Fragments arranged by size: 2579 1830 1247 285 Bpu10I CC'TnA_GC Cuts at: 2936 2936 Size: 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2212 2263 4066 4931 5229 2212 Size: 51 1803 865 298 2924 Fragments arranged by size: 2924 1803 865 298 51 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2583 2773 3238 3732 493 Size: 711 1379 190 465 494 2702 Fragments arranged by size: 2702 1379 711 494 465 190 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 2518 3748 4130 Size: 53 83 186 936 515 470 1230 382 Cuts at: 4130 4765 275 Size: 635 1451 Fragments arranged by size: 1451 1230 936 635 515 470 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2234 2264 2414 2444 2583 Size: 30 165 30 831 30 150 30 139 Cuts at: 2583 2613 1178 Size: 30 4506 Fragments arranged by size: 4506 831 165 150 139 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2255 2495 1186 Size: 922 147 240 4632 Fragments arranged by size: 4632 922 240 147 BseYI C'CCAG_C Cuts at: 2335 2866 5144 5640 2335 Size: 531 2278 496 2636 Fragments arranged by size: 2636 2278 531 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 1163 Size: 573 366 5002 Fragments arranged by size: 5002 573 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2697 3078 3132 4002 4087 5158 2697 Size: 381 54 870 85 1071 3480 Fragments arranged by size: 3480 1071 870 381 85 54 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2747 2747 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2697 2912 2922 2985 3078 3132 Size: 399 443 464 215 10 63 93 54 Cuts at: 3132 3260 4002 4087 5158 1391 Size: 128 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 464 443 399 215 128 93 85 63 54 10 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2790 3296 844 Size: 1946 506 3489 Fragments arranged by size: 3489 1946 506 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2514 2721 4585 4772 1700 Size: 110 704 207 1864 187 2869 Fragments arranged by size: 2869 1864 704 207 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 2795 4001 5013 2795 Size: 1206 1012 3723 Fragments arranged by size: 3723 1206 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 2007 4745 2007 Size: 2738 3203 BstEII G'GTnAC_C Cuts at: 1184 2284 1184 Size: 1100 4841 BstXI CCAn_nnnn'nTGG Cuts at: 2335 2335 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2187 3271 3638 4023 4040 5492 5578 5590 Size: 215 1084 367 385 17 1452 86 12 Cuts at: 5590 5936 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1084 385 367 346 215 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2403 513 Size: 552 957 381 4051 Fragments arranged by size: 4051 957 552 381 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2955 3109 3457 4251 4271 2955 Size: 154 348 794 20 4625 Fragments arranged by size: 4625 794 348 154 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2270 4948 809 Size: 507 954 2678 1802 Fragments arranged by size: 2678 1802 954 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2325 2643 Size: 54 1425 50 275 18 274 221 318 Cuts at: 2643 2964 2991 3388 4277 5906 8 Size: 321 27 397 889 1629 43 Fragments arranged by size: 1629 1425 889 397 321 318 275 274 221 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3027 3871 1353 Size: 1674 844 3423 Fragments arranged by size: 3423 1674 844 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2695 3130 2695 Size: 435 5506 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2486 2570 2771 4004 4601 5388 5656 Size: 393 854 84 201 1233 597 787 268 Cuts at: 5656 1239 Size: 1524 Fragments arranged by size: 1524 1233 854 787 597 393 268 201 84 EcoNI CCTnn'n_nnAGG Cuts at: 3032 3032 Size: 5941 EcoO109I rG'GnC_Cy Cuts at: 2272 3057 3205 2272 Size: 785 148 5008 Fragments arranged by size: 5008 785 148 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 2456 4447 2456 Size: 1991 3950 HaeII r_GCGC'y Cuts at: 1678 2652 5088 1678 Size: 974 2436 2531 Fragments arranged by size: 2531 2436 974 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2608 2640 4582 4614 4656 Size: 32 135 32 1036 32 1942 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 1942 1036 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2031 5055 5239 1305 Size: 726 3024 184 2007 Fragments arranged by size: 3024 2007 726 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 2993 10 Size: 54 1425 343 274 887 2958 Fragments arranged by size: 2958 1425 887 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2771 2903 3899 4258 4417 518 Size: 648 1605 132 996 359 159 2042 Fragments arranged by size: 2042 1605 996 648 359 159 132 MspA1I CmG'CkG Cuts at: 1606 2405 2866 3357 4034 5182 5427 1606 Size: 799 461 491 677 1148 245 2120 Fragments arranged by size: 2120 1148 799 677 491 461 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4432 Fragments arranged by size: 4432 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2848 3299 4844 1132 Size: 97 1619 451 1545 2229 Fragments arranged by size: 2229 1619 1545 451 97 PasI CC'CwG_GG Cuts at: 1348 2878 1348 Size: 1530 4411 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2175 2848 1108 Size: 1067 673 4201 Fragments arranged by size: 4201 1067 673 PfoI T'CCnGG_A Cuts at: 2463 2463 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PmlI CAC'GTG Cuts at: 2583 2773 2583 Size: 190 5751 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2272 3057 3205 2272 Size: 785 148 5008 Fragments arranged by size: 5008 785 148 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PstI C_TGCA'G Cuts at: 830 2217 2569 3293 830 Size: 1387 352 724 3478 Fragments arranged by size: 3478 1387 724 352 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2866 2866 Size: 5941 SacI G_AGCT'C Cuts at: 2697 3132 2697 Size: 435 5506 SacII CC_GC'GG Cuts at: 2406 2406 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 3057 3057 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2213 2565 3142 3289 4424 5105 5296 Size: 219 1168 352 577 147 1135 681 191 Cuts at: 5296 826 Size: 1471 Fragments arranged by size: 1471 1168 1135 681 577 352 219 191 147 SfiI GGCCn_nnn'nGGCC Cuts at: 2999 2999 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2075 2156 3268 1872 Size: 203 81 1112 4545 Fragments arranged by size: 4545 1112 203 81 SmlI C'TyrA_G Cuts at: 820 1017 1943 2191 2278 4045 4946 5208 820 Size: 197 926 248 87 1767 901 262 1553 Fragments arranged by size: 1767 1553 926 901 262 248 197 87 SnaBI TAC'GTA Cuts at: 493 3238 3732 493 Size: 2745 494 2702 Fragments arranged by size: 2745 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SrfI GCCC'GGGC Cuts at: 2156 2156 Size: 5941 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 3017 1517 Size: 1500 4441 StyI C'CwwG_G Cuts at: 513 1065 2022 2559 3380 513 Size: 552 957 537 821 3074 Fragments arranged by size: 3074 957 821 552 537 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2965 3968 3985 4138 4323 4768 325 Size: 1255 1385 1003 17 153 185 445 1498 Fragments arranged by size: 1498 1385 1255 1003 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 4187 5899 Size: 274 80 33 51 1443 333 1877 1712 Cuts at: 5899 96 Size: 138 Fragments arranged by size: 1877 1712 1443 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 2583 2615 5901 5933 2414 Size: 32 137 32 3286 32 2422 Fragments arranged by size: 3286 2422 137 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2233 2974 2233 Size: 741 5200 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2073 2154 3266 1870 Size: 203 81 1112 4545 Fragments arranged by size: 4545 1112 203 81 XmnI GAAnn'nnTTC Cuts at: 2145 4070 2145 Size: 1925 4016 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI SexAI SfoI SwaI