(Circular) (Six-base) MAPSORT of: pFN21ASDA1831.seq Check: 2919 from: 1 to: 6613 pFN21ASDA1831 6613 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2722 4423 278 Size: 53 83 186 936 1186 1701 2468 Fragments arranged by size: 2468 1701 1186 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3956 1937 Size: 2019 4594 Acc65I G'GTAC_C Cuts at: 3934 3934 Size: 6613 AclI AA'CG_TT Cuts at: 1563 4740 5113 1563 Size: 3177 373 3063 Fragments arranged by size: 3177 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 4676 6060 6328 1632 Size: 683 2361 1384 268 1917 Fragments arranged by size: 2361 1917 1384 683 268 AfeI AGC'GCT Cuts at: 2918 2918 Size: 6613 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6416 AflIII A'CryG_T Cuts at: 1203 5512 6489 1203 Size: 4309 977 1327 Fragments arranged by size: 4309 1327 977 AgeI A'CCGG_T Cuts at: 5444 5444 Size: 6613 AhdI GACnn_n'nnGTC Cuts at: 2638 5342 2638 Size: 2704 3909 AleI CACnn'nnGTG Cuts at: 1166 2861 1166 Size: 1695 4918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3077 1184 Size: 1893 4720 AlwNI CAG_nnn'CTG Cuts at: 2145 3268 4356 5928 2145 Size: 1123 1088 1572 2830 Fragments arranged by size: 2830 1572 1123 1088 ApaI G_GGCC'C Cuts at: 2120 2190 2622 2817 3597 3697 2120 Size: 70 432 195 780 100 5036 Fragments arranged by size: 5036 780 432 195 100 70 ApaLI G'TGCA_C Cuts at: 2110 3035 4670 5826 2110 Size: 925 1635 1156 2897 Fragments arranged by size: 2897 1635 1156 925 ApoI r'AATT_y Cuts at: 1493 3922 4148 4300 6409 1493 Size: 2429 226 152 2109 1697 Fragments arranged by size: 2429 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5167 160 Size: 872 4135 1606 Fragments arranged by size: 4135 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2587 2595 2621 3751 3788 3938 Size: 73 87 557 8 26 1130 37 150 Cuts at: 3938 1870 Size: 4545 Fragments arranged by size: 4545 1130 557 150 87 73 37 26 8 BamHI G'GATC_C Cuts at: 3389 3943 4310 3389 Size: 554 367 5692 Fragments arranged by size: 5692 554 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2402 3119 3771 3934 Size: 325 217 627 336 279 717 652 163 Cuts at: 3934 5389 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 717 652 627 336 325 279 217 163 BanII G_rGCy'C Cuts at: 1391 2120 2190 2349 2594 2622 2706 2817 2852 Size: 729 70 159 245 28 84 111 35 Cuts at: 2852 3336 3458 3597 3678 3697 3720 3746 3789 Size: 484 122 139 81 19 23 26 43 Cuts at: 3789 3798 3932 1391 Size: 9 134 4072 Fragments arranged by size: 4072 729 484 245 159 139 134 122 111 84 81 70 43 35 28 26 23 19 9 BbeI G_GCGC'C Cuts at: 2127 3123 2127 Size: 996 5617 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2170 3638 928 Size: 971 271 1468 3903 Fragments arranged by size: 3903 1468 971 271 BbvCI CC'TCA_GC Cuts at: 3564 3689 3872 3564 Size: 125 183 6305 Fragments arranged by size: 6305 183 125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2884 2918 3797 3831 4804 4838 1903 Size: 34 947 34 879 34 973 34 3678 Fragments arranged by size: 3678 973 947 879 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4505 5715 4505 Size: 1210 5403 BclI T'GATC_A Cuts at: 1548 1548 Size: 6613 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2994 3233 5224 Size: 107 122 71 631 605 1322 239 1991 Cuts at: 5224 136 Size: 1525 Fragments arranged by size: 1991 1525 1322 631 605 239 122 107 71 BglII A'GATC_T Cuts at: 6608 6608 Size: 6613 BlpI GC'TnA_GC Cuts at: 2696 4030 2696 Size: 1334 5279 Bme1580I G_kGCm'C Cuts at: 1790 2095 2114 2120 2155 2190 2466 2622 2817 Size: 305 19 6 35 35 276 156 195 Cuts at: 2817 3039 3597 3697 4674 5830 1790 Size: 222 558 100 977 1156 2573 Fragments arranged by size: 2573 1156 977 558 305 276 222 195 156 100 35 35 19 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6613 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2180 4344 5302 Size: 416 53 83 399 24 763 2164 958 Cuts at: 5302 442 Size: 1753 Fragments arranged by size: 2164 1753 958 763 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6613 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2407 2407 Size: 6613 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3475 3489 5273 1239 Size: 2236 14 1784 2579 Fragments arranged by size: 2579 2236 1784 14 Bpu10I CC'TnA_GC Cuts at: 3564 3689 3714 3872 3564 Size: 125 25 158 6305 Fragments arranged by size: 6305 158 125 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4738 5603 5901 4738 Size: 865 298 5450 Fragments arranged by size: 5450 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2378 5276 882 Size: 617 879 2898 2219 Fragments arranged by size: 2898 2219 879 617 BsaAI yAC'GTr Cuts at: 493 1204 2068 3910 4404 493 Size: 711 864 1842 494 2702 Fragments arranged by size: 2702 1842 864 711 494 BsaBI GATnn'nnATC Cuts at: 6607 6607 Size: 6613 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 2175 2537 2628 Size: 53 83 186 936 591 51 362 91 Cuts at: 2628 2719 2907 3120 3183 4420 4802 5437 275 Size: 91 188 213 63 1237 382 635 1451 Fragments arranged by size: 1451 1237 936 635 591 382 362 213 188 186 91 91 83 63 53 51 BsaWI w'CCGG_w Cuts at: 2786 5046 5444 5718 5865 6276 2786 Size: 2260 398 274 147 411 3123 Fragments arranged by size: 3123 2260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2999 3029 1178 Size: 30 165 30 1596 30 4762 Fragments arranged by size: 4762 1596 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3020 3409 3527 3605 3668 3671 3890 1186 Size: 1834 389 118 78 63 3 219 3909 Fragments arranged by size: 3909 1834 389 219 118 78 63 3 BseYI C'CCAG_C Cuts at: 2076 2148 2239 2284 2950 3404 3575 3697 3767 Size: 72 91 45 666 454 171 122 70 Cuts at: 3767 3819 3894 5816 6312 2076 Size: 52 75 1922 496 2377 Fragments arranged by size: 2377 1922 666 496 454 171 122 91 75 72 70 52 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6040 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2667 2823 4063 4824 5852 1540 Size: 275 186 666 156 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 666 275 186 156 BsiHKAI G_wGCw'C Cuts at: 2114 3039 3789 4674 4759 5830 2114 Size: 925 750 885 85 1071 2897 Fragments arranged by size: 2897 1071 925 885 750 85 BsmI GAATG_Cn' Cuts at: 3881 4124 4217 3881 Size: 243 93 6277 Fragments arranged by size: 6277 243 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3594 3594 Size: 6613 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2114 2120 2155 2190 2349 2466 Size: 399 305 19 6 35 35 159 117 Cuts at: 2466 2594 2622 2706 2817 2852 3039 3336 3458 Size: 128 28 84 111 35 187 297 122 Cuts at: 3458 3597 3678 3697 3720 3746 3789 3798 3932 Size: 139 81 19 23 26 43 9 134 Cuts at: 3932 4674 4759 5830 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 305 297 187 159 139 134 128 122 117 111 85 84 81 43 35 35 35 28 26 23 19 19 9 6 BspEI T'CCGG_A Cuts at: 6276 6276 Size: 6613 BspHI T'CATG_A Cuts at: 4502 6232 4502 Size: 1730 4883 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3276 3968 844 Size: 2432 692 3489 Fragments arranged by size: 3489 2432 692 BsrBI CCG'CTC Cuts at: 1394 1823 2711 4500 1394 Size: 429 888 1789 3507 Fragments arranged by size: 3507 1789 888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5108 5282 65 Size: 1183 3860 174 1396 Fragments arranged by size: 3860 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2666 2958 3027 5257 5444 1700 Size: 110 856 292 69 2230 187 2869 Fragments arranged by size: 2869 2230 856 292 187 110 69 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6613 BssHII G'CGCG_C Cuts at: 1925 2881 1925 Size: 956 5657 BssSI C'ACGA_G Cuts at: 4673 5685 4673 Size: 1012 5601 BstAPI GCAn_nnn'nTGC Cuts at: 794 3277 794 Size: 2483 4130 BstBI TT'CG_AA Cuts at: 2007 5417 2007 Size: 3410 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6613 BstXI CCAn_nnnn'nTGG Cuts at: 3841 3841 Size: 6613 BstYI r'GATC_y Cuts at: 1972 3389 3943 4310 4695 4712 6164 6250 6262 Size: 1417 554 367 385 17 1452 86 12 Cuts at: 6262 6608 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1417 554 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3510 3737 3510 Size: 227 6386 BtgI C'CryG_G Cuts at: 513 1065 2272 2437 3228 3855 513 Size: 552 1207 165 791 627 3271 Fragments arranged by size: 3271 1207 791 627 552 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3706 487 Size: 683 56 2480 3394 Fragments arranged by size: 3394 2480 683 56 BtsI GCAGTG_nn' Cuts at: 3204 3217 3458 4129 4923 4943 3204 Size: 13 241 671 794 20 4874 Fragments arranged by size: 4874 794 671 241 20 13 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6613 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6578 DraI TTT'AAA Cuts at: 3917 3917 Size: 6613 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3110 5620 809 Size: 507 1794 2510 1802 Fragments arranged by size: 2510 1802 1794 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2518 2613 2664 Size: 54 1425 50 275 18 688 95 51 Cuts at: 2664 2820 2853 3246 4060 4949 6578 8 Size: 156 33 393 814 889 1629 43 Fragments arranged by size: 1629 1425 889 814 688 393 275 156 95 54 51 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2664 2820 4060 1537 Size: 275 852 156 1240 4090 Fragments arranged by size: 4090 1240 852 275 156 EarI CTCTTCn'nnn_ Cuts at: 1353 3666 3777 4543 1353 Size: 2313 111 766 3423 Fragments arranged by size: 3423 2313 766 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2208 2685 2730 5200 5574 5720 Size: 1064 138 619 477 45 2470 374 146 Cuts at: 5720 387 Size: 1280 Fragments arranged by size: 2470 1280 1064 619 477 374 146 138 45 EcoICRI GAG'CTC Cuts at: 3787 3787 Size: 6613 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 3475 3489 4676 5273 6060 6328 Size: 393 683 1160 14 1187 597 787 268 Cuts at: 6328 1239 Size: 1524 Fragments arranged by size: 1524 1187 1160 787 683 597 393 268 14 EcoNI CCTnn'n_nnAGG Cuts at: 2843 3191 3341 3468 2843 Size: 348 150 127 5988 Fragments arranged by size: 5988 348 150 127 EcoO109I rG'GnC_Cy Cuts at: 2117 2186 2814 3265 3286 3482 3593 3693 3694 Size: 69 628 451 21 196 111 100 1 Cuts at: 3694 3758 2117 Size: 64 4972 Fragments arranged by size: 4972 628 451 196 111 100 69 64 21 1 EcoRI G'AATT_C Cuts at: 3922 4300 3922 Size: 378 6235 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2062 2062 Size: 6613 FspI TGC'GCA Cuts at: 2756 5119 2756 Size: 2363 4250 HaeII r_GCGC'y Cuts at: 1678 2127 2437 2483 2831 2920 3123 3154 5760 Size: 449 310 46 348 89 203 31 2606 Cuts at: 5760 1678 Size: 2531 Fragments arranged by size: 2606 2531 449 348 310 203 89 46 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 5254 5286 5328 Size: 32 135 32 1052 32 2598 32 42 Cuts at: 5328 5360 1373 Size: 32 2626 Fragments arranged by size: 2626 2598 1052 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3957 4203 669 Size: 988 281 2019 246 3079 Fragments arranged by size: 3079 2019 988 281 246 HindIII A'AGCT_T Cuts at: 748 3501 748 Size: 2753 3860 HpaI GTT'AAC Cuts at: 4203 4203 Size: 6613 KasI G'GCGC_C Cuts at: 2123 3119 2123 Size: 996 5617 KpnI G_GTAC'C Cuts at: 3938 3938 Size: 6613 MfeI C'AATT_G Cuts at: 4212 4212 Size: 6613 MluI A'CGCG_T Cuts at: 6489 6489 Size: 6613 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2418 2946 3348 5727 5911 1305 Size: 1113 528 402 2379 184 2007 Fragments arranged by size: 2379 2007 1113 528 402 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3248 10 Size: 54 1425 343 1416 3375 Fragments arranged by size: 3375 1425 1416 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 3725 4571 4930 5089 518 Size: 648 1695 864 846 359 159 2042 Fragments arranged by size: 2042 1695 864 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2142 2262 2274 2307 2700 2811 2898 3167 Size: 536 120 12 33 393 111 87 269 Cuts at: 3167 3688 3800 4029 4706 5854 6099 1606 Size: 521 112 229 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 536 521 393 269 245 229 120 112 111 87 33 12 NaeI GCC'GGC Cuts at: 2668 2668 Size: 6613 NarI GG'CG_CC Cuts at: 2124 3120 2124 Size: 996 5617 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6061 NdeI CA'TA_TG Cuts at: 387 387 Size: 6613 NgoMIV G'CCGG_C Cuts at: 2666 2666 Size: 6613 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6613 NotI GC'GGCC_GC Cuts at: 4060 4060 Size: 6613 NspI r_CATG'y Cuts at: 1132 1229 3971 5516 1132 Size: 97 2742 1545 2229 Fragments arranged by size: 2742 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2128 2731 3353 1348 Size: 780 603 622 4608 Fragments arranged by size: 4608 780 622 603 PciI A'CATG_T Cuts at: 5512 5512 Size: 6613 PflMI CCAn_nnn'nTGG Cuts at: 1108 3504 1108 Size: 2396 4217 PfoI T'CCnGG_A Cuts at: 2364 3466 3864 2364 Size: 1102 398 5113 Fragments arranged by size: 5113 1102 398 PmeI GTTT'AAAC Cuts at: 3917 3917 Size: 6613 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4682 4714 6195 6227 4682 Size: 32 1481 32 5068 Fragments arranged by size: 5068 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3265 3482 3758 3265 Size: 217 276 6120 Fragments arranged by size: 6120 276 217 PshAI GACnn'nnGTC Cuts at: 1613 3480 1613 Size: 1867 4746 PsiI TTA'TAA Cuts at: 4183 4183 Size: 6613 PspOMI G'GGCC_C Cuts at: 2116 2186 2618 2813 3593 3693 2116 Size: 70 432 195 780 100 5036 Fragments arranged by size: 5036 780 432 195 100 70 PspXI vC'TCGA_Gb Cuts at: 3751 3751 Size: 6613 PstI C_TGCA'G Cuts at: 830 3273 3629 3965 830 Size: 2443 356 336 3478 Fragments arranged by size: 3478 2443 356 336 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6613 RsrII CG'GwC_CG Cuts at: 2789 2789 Size: 6613 SacI G_AGCT'C Cuts at: 3789 3789 Size: 6613 SacII CC_GC'GG Cuts at: 2275 2275 Size: 6613 SalI G'TCGA_C Cuts at: 1936 3955 1936 Size: 2019 4594 SanDI GG'GwC_CC Cuts at: 3482 3482 Size: 6613 SapI GCTCTTCn'nnn_ Cuts at: 3666 3777 3666 Size: 111 6502 SbfI CC_TGCA'GG Cuts at: 3965 3965 Size: 6613 ScaI AGT'ACT Cuts at: 4861 6573 4861 Size: 1712 4901 SfcI C'TryA_G Cuts at: 826 1045 3269 3625 3961 5096 5777 5968 826 Size: 219 2224 356 336 1135 681 191 1471 Fragments arranged by size: 2224 1471 1135 681 356 336 219 191 SfoI GGC'GCC Cuts at: 2125 3121 2125 Size: 996 5617 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6613 SmaI CCC'GGG Cuts at: 1872 2032 2597 2623 3940 1872 Size: 160 565 26 1317 4545 Fragments arranged by size: 4545 1317 565 160 26 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4717 5618 5880 820 Size: 197 926 1808 966 901 262 1553 Fragments arranged by size: 1808 1553 966 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3910 4404 493 Size: 3417 494 2702 Fragments arranged by size: 3417 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6613 SphI G_CATG'C Cuts at: 1132 3971 1132 Size: 2839 3774 SspI AAT'ATT Cuts at: 5 52 4537 5 Size: 47 4485 2081 Fragments arranged by size: 4485 2081 47 StuI AGG'CCT Cuts at: 1517 3814 1517 Size: 2297 4316 StyI C'CwwG_G Cuts at: 513 1065 4052 513 Size: 552 2987 3074 Fragments arranged by size: 3074 2987 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3759 4640 4657 4810 4995 5440 325 Size: 1255 2179 881 17 153 185 445 1498 Fragments arranged by size: 2179 1498 1255 881 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 3015 4859 Size: 274 80 33 51 1443 159 879 1844 Cuts at: 4859 6571 96 Size: 1712 138 Fragments arranged by size: 1844 1712 1443 879 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6573 6605 6573 Size: 32 6581 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6613 XbaI T'CTAG_A Cuts at: 3949 3949 Size: 6613 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3727 3841 3727 Size: 114 6499 XhoI C'TCGA_G Cuts at: 1943 3751 1943 Size: 1808 4805 XmaI C'CCGG_G Cuts at: 1870 2030 2595 2621 3938 1870 Size: 160 565 26 1317 4545 Fragments arranged by size: 4545 1317 565 160 26 XmnI GAAnn'nnTTC Cuts at: 4742 4742 Size: 6613 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2720 4421 276 Size: 53 83 186 936 1186 1701 2468 Fragments arranged by size: 2468 1701 1186 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI PvuII SexAI SfiI SrfI SwaI