Restriction map for
pFN21ASDA1830
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HpaI HpaII
KasI KpnI KspI MaeII MfeI
MluI MroI MspI MunI MvaI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 30 GCGG CCGC(-3/-1)
AseI 1006 ATTAAT AT^TAAT
AsnI 1006 ATTAAT AT^TAAT
AspHI 1116 GAGCTC G[AT]GC[AT]^C
AspI 991 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 988 GAAGAC GAAGAC(2/6)
BpmI 224 CTCCAG CTGGAG(16/14)
BpuAI 988 GAAGAC GAAGAC(2/6)
BsaBI 646 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 833 GAGACC GGTCTC(1/5)
BsgI 1324 GTGCAG GTGCAG(16/14)
BsiHKAI 1116 GAGCTC G[AT]GC[AT]^C
BsrDI 267 CATTGC GCAATG(2/0)
BstXI 864 CCACATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1306 GGATCT [GA]^GATC[CT]
DraI 103 TTTAAA TTT^AAA
DraIII 1154 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 151 CCATGG C^C[GA][CT]GG
HincII 416 GTCAAC GT[CT]^[GA]AC
HindII 416 GTCAAC GT[CT]^[GA]AC
HindIII 739 AAGCTT A^AGCTT
MaeIII 574 GTGAC ^GT[ACGT]AC
MamI 646 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1306 GGATCT [GA]^GATC[CT]
MslI 865 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 593 CAGCTG C[AC]G^C[GT]G
NcoI 151 CCATGG C^CATGG
NdeI 433 CATATG CA^TATG
PflFI 991 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 151 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1006 ATTAAT AT^TAAT
PvuII 593 CAGCTG CAG^CTG
SacI 1116 GAGCTC GAGCT^C
SmlI 202 CTTGAG C^T[CT][GA]AG
SspI 953 AATATT AAT^ATT
Tsp45I 574 GTGAC ^GT[GC]AC
Tth111I 991 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 151 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1306 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 52 GTAC GT^AC
931 GTAC
AlwI 665 GGATC GGATC(4/5)
1306 GGATC
Asp700I 556 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
676 GAAAGGTTTC
AvaII 343 GGACC G^G[AT]CC
996 GGTCC
BalI 159 TGGCCA TGG^CCA
861 TGGCCA
BfaI 263 CTAG C^TAG
964 CTAG
BsmFI 320 GGGAC GGGAC(10/14)
916 GTCCC
Cac8I 591 GCCAGC GC[ACGT]^[ACGT]GC
1148 GCCTGC
CfoI 38 GCGC GCG^C
148 GCGC
DdeI 549 CTCAG C^T[ACGT]AG
566 CTGAG
HhaI 38 GCGC GCG^C
148 GCGC
HinP1I 38 GCGC G^CGC
148 GCGC
MaeI 263 CTAG C^TAG
964 CTAG
MluNI 159 TGGCCA TGG^CCA
861 TGGCCA
MscI 159 TGGCCA TGG^CCA
861 TGGCCA
MwoI 591 GCCAGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1317 GCCTACAGTGC
RsaI 52 GTAC GT^AC
931 GTAC
TaqI 114 TCGA T^CGA
288 TCGA
TthHB8I 114 TCGA T^CGA
288 TCGA
XmnI 556 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
676 GAAAGGTTTC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI BanII BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BstF5I Cfr13I DpnII EaeI EarI
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MboI MboII
MnlI MseI NdeII NlaIII NlaIV
Sau3AI Sau96I SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI