Restriction map for
pFN21ASDA1830
(Circular) (Six-base) MAPSORT of: pFN21ASDA1830.seq Check: 4205 from: 1 to: 6091
pFN21ASDA1830 6091 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2280 2520 4154 5538 5806 1632
Size: 648 240 1634 1384 268 1917
Fragments arranged by size:
1917 1634 1384 648 268 240
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3030 4645 160
Size: 872 1998 1615 1606
Fragments arranged by size:
1998 1615 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3416 1870
Size: 73 1473 4545
Fragments arranged by size:
4545 1473 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2419 3143 3318 3357 3410 1391
Size: 1028 724 175 39 53 4072
Fragments arranged by size:
4072 1028 724 175 53 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3018 928
Size: 971 1119 4001
Fragments arranged by size:
4001 1119 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 4152 5308 1790
Size: 2362 1156 2573
Fragments arranged by size:
2573 2362 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3188 3822 4780
Size: 416 53 83 399 24 1771 634 958
Cuts at: 4780 442
Size: 1753
Fragments arranged by size:
1771 1753 958 634 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 4751 1239
Size: 993 2519 2579
Fragments arranged by size:
2579 2519 993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2246 4216 5081 5379 2246
Size: 1970 865 298 2958
Fragments arranged by size:
2958 1970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2850 4754 882
Size: 617 1351 1904 2219
Fragments arranged by size:
2219 1904 1351 617
BsaAI yAC'GTr
Cuts at: 493 1204 3388 3882 493
Size: 711 2184 494 2702
Fragments arranged by size:
2702 2184 711 494
BsaBI GATnn'nnATC
Cuts at: 2673 6085 2673
Size: 3412 2679
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3318 3348 1178
Size: 30 165 30 1915 30 3921
Fragments arranged by size:
3921 1915 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6091
BseYI C'CCAG_C
Cuts at: 3317 5294 5790 3317
Size: 1977 496 3618
Fragments arranged by size:
3618 1977 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3368 1163
Size: 573 1632 3886
Fragments arranged by size:
3886 1632 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3143 4152 4237 5308 3143
Size: 1009 85 1071 3926
Fragments arranged by size:
3926 1071 1009 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2419 3143 3318 3357 3410 4152 4237
Size: 399 629 724 175 39 53 742 85
Cuts at: 4237 5308 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 724 629 399 175 85
53 39
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 4586 4760 65
Size: 1183 1041 2297 174 1396
Fragments arranged by size:
2297 1396 1183 1041 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 2007 4895 2007
Size: 2888 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 2894 2894
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 3329 3421 3788 4173 4190 5642 5728 5740
Size: 1357 92 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1357 385 367 346 92 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2174 513
Size: 552 957 152 4430
Fragments arranged by size:
4430 957 552 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 3607 4401 4421 3328
Size: 279 794 20 4998
Fragments arranged by size:
4998 794 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2053 2088 529
Size: 35 1489 35 4532
Fragments arranged by size:
4532 1489 35 35
DraI TTT'AAA
Cuts at: 2128 3395 2128
Size: 1267 4824
DraIII CAC_nnn'GTG
Cuts at: 3182 3182
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2426 2884
Size: 54 1425 50 275 18 352 244 458
Cuts at: 2884 3538 4427 6056 8
Size: 654 889 1629 43
Fragments arranged by size:
1629 1425 889 654 458 352 275 244
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2459 2867 3288 4021 1353
Size: 677 429 408 421 733 3423
Fragments arranged by size:
3423 733 677 429 421 408
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3141 3141
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2280 2520 4154 4751 5538 5806
Size: 393 600 48 240 1634 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1634 1524 787 600 597 393 268 240
48
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2240 2240
Size: 6091
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2666 2698 3098 3130 4732
Size: 32 135 32 1094 32 400 32 1602
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1602 1094 400 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2441 3435 3681 669
Size: 988 281 503 994 246 3079
Fragments arranged by size:
3079 994 988 503 281 246
HindIII A'AGCT_T
Cuts at: 748 2762 748
Size: 2014 4077
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2021 2239 2345 5205 5389 1305
Size: 716 218 106 2860 184 2007
Fragments arranged by size:
2860 2007 716 218 184 106
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2184 2886 10
Size: 54 1425 343 352 702 3215
Fragments arranged by size:
3215 1425 702 352 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2892 4049 4408 4567 518
Size: 648 1726 1157 359 159 2042
Fragments arranged by size:
2042 1726 1157 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2618 3507 4184 5332 5577 1606
Size: 1012 889 677 1148 245 2120
Fragments arranged by size:
2120 1148 1012 889 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2174 513
Size: 552 957 152 4430
Fragments arranged by size:
4430 957 552 152
NdeI CA'TA_TG
Cuts at: 387 2457 387
Size: 2070 4021
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3449 4994 1132
Size: 97 2220 1545 2229
Fragments arranged by size:
2229 2220 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2180 1108
Size: 1072 5019
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2618 2618
Size: 6091
SacI G_AGCT'C
Cuts at: 3143 3143
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2946 3333 3342 3439 4574 5255 5446
Size: 219 1901 387 9 97 1135 681 191
Cuts at: 5446 826
Size: 1471
Fragments arranged by size:
1901 1471 1135 681 387 219 191 97
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 4195 5096 5358 820
Size: 197 926 282 1970 901 262 1553
Fragments arranged by size:
1970 1553 926 901 282 262 197
SnaBI TAC'GTA
Cuts at: 493 3388 3882 493
Size: 2895 494 2702
Fragments arranged by size:
2895 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 2978 4015 5
Size: 47 2926 1037 2081
Fragments arranged by size:
2926 2081 1037 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2022 2174 3061 3357 3530 513
Size: 552 957 152 887 296 173 3074
Fragments arranged by size:
3074 957 887 552 296 173 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3170 4118 4135 4288 4473 4918 325
Size: 1255 1590 948 17 153 185 445 1498
Fragments arranged by size:
1590 1498 1255 948 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 2953 4337
Size: 274 80 33 51 1443 97 879 1384
Cuts at: 4337 6049 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1384 879 274 138 97 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2751 2783 3321 3353 6051 6083 2751
Size: 32 538 32 2698 32 2759
Fragments arranged by size:
2759 2698 538 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3017 1316
Size: 1701 4390
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 2703 4220 2583
Size: 120 1517 4454
Fragments arranged by size:
4454 1517 120
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI