Restriction map for
pFN21ASDA1830
(Linear) (Six-base) MAPSORT of: KIAA1830.seq Check: 4364 from: 1 to: 1362
KIAA1830.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 497 1362
Size: 257 240 865
Fragments arranged by size:
865 257 240
AseI AT'TA_AT
Cuts at: 0 1007 1362
Size: 1007 355
BanII G_rGCy'C
Cuts at: 0 396 1120 1295 1334 1362
Size: 396 724 175 39 28
Fragments arranged by size:
724 396 175 39 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 995 1362
Size: 995 367
BmrI ACTGGGnnnn_n'
Cuts at: 0 1165 1362
Size: 1165 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1362
Size: 209 1153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1362
Size: 223 1139
BsaI GGTCTCn'nnnn_
Cuts at: 0 827 1362
Size: 827 535
BsaBI GATnn'nnATC
Cuts at: 0 650 1362
Size: 650 712
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1295 1325 1362
Size: 1295 30 37
Fragments arranged by size:
1295 37 30
BseYI C'CCAG_C
Cuts at: 0 1294 1362
Size: 1294 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1362
Size: 1345 17
BsiHKAI G_wGCw'C
Cuts at: 0 1120 1362
Size: 1120 242
Bsp1286I G_dGCh'C
Cuts at: 0 396 1120 1295 1334 1362
Size: 396 724 175 39 28
Fragments arranged by size:
724 396 175 39 28
BsrDI GCAATG_nn'
Cuts at: 0 266 1362
Size: 266 1096
BstXI CCAn_nnnn'nTGG
Cuts at: 0 871 1362
Size: 871 491
BstYI r'GATC_y
Cuts at: 0 1306 1362
Size: 1306 56
BtgI C'CryG_G
Cuts at: 0 151 1362
Size: 151 1211
BtsI GCAGTG_nn'
Cuts at: 0 1305 1362
Size: 1305 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 30 65 1362
Size: 30 35 1297
Fragments arranged by size:
1297 35 30
DraI TTT'AAA
Cuts at: 0 105 1362
Size: 105 1257
DraIII CAC_nnn'GTG
Cuts at: 0 1159 1362
Size: 1159 203
EaeI y'GGCC_r
Cuts at: 0 159 403 861 1362
Size: 159 244 458 501
Fragments arranged by size:
501 458 244 159
EarI CTCTTCn'nnn_
Cuts at: 0 7 436 844 1265 1362
Size: 7 429 408 421 97
Fragments arranged by size:
429 421 408 97 7
EcoICRI GAG'CTC
Cuts at: 0 1118 1362
Size: 1118 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 257 497 1362
Size: 209 48 240 865
Fragments arranged by size:
865 240 209 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 217 1362
Size: 217 1145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 1075 1107 1362
Size: 643 32 400 32 255
Fragments arranged by size:
643 400 255 32 32
HincII GTy'rAC
Cuts at: 0 418 1362
Size: 418 944
HindIII A'AGCT_T
Cuts at: 0 739 1362
Size: 739 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 322 1362
Size: 216 106 1040
Fragments arranged by size:
1040 216 106
MscI TGG'CCA
Cuts at: 0 161 863 1362
Size: 161 702 499
Fragments arranged by size:
702 499 161
MslI CAynn'nnrTG
Cuts at: 0 869 1362
Size: 869 493
MspA1I CmG'CkG
Cuts at: 0 595 1362
Size: 595 767
NcoI C'CATG_G
Cuts at: 0 151 1362
Size: 151 1211
NdeI CA'TA_TG
Cuts at: 0 434 1362
Size: 434 928
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 1362
Size: 157 1205
PvuII CAG'CTG
Cuts at: 0 595 1362
Size: 595 767
SacI G_AGCT'C
Cuts at: 0 1120 1362
Size: 1120 242
SfcI C'TryA_G
Cuts at: 0 923 1310 1319 1362
Size: 923 387 9 43
Fragments arranged by size:
923 387 43 9
SmlI C'TyrA_G
Cuts at: 0 202 1362
Size: 202 1160
SspI AAT'ATT
Cuts at: 0 955 1362
Size: 955 407
StyI C'CwwG_G
Cuts at: 0 151 1038 1334 1362
Size: 151 887 296 28
Fragments arranged by size:
887 296 151 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1147 1362
Size: 1147 215
TatI w'GTAC_w
Cuts at: 0 51 930 1362
Size: 51 879 432
Fragments arranged by size:
879 432 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 728 760 1298 1330 1362
Size: 728 32 538 32 32
Fragments arranged by size:
728 538 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 994 1362
Size: 994 368
XmnI GAAnn'nnTTC
Cuts at: 0 560 680 1362
Size: 560 120 682
Fragments arranged by size:
682 560 120
Enzymes that do cut:
AcuI AseI BanII BbsI BmrI BpmI BpuEI BsaI
BsaBI BsaXI BseYI BsgI BsiHKAI Bsp1286I BsrDI BstXI
BstYI BtgI BtsI CspCI DraI DraIII EaeI EarI
EcoICRI Eco57MI FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NcoI NdeI PflMI PvuII SacI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaHI
BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XcmI XhoI XmaI ZraI