Restriction map for
pFN21ASDA1823
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MflI MluI MluNI MroI
MscI MslI MspI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 292 ACATGT A^C[GA][CT]GT
AvaII 539 GGTCC G^G[AT]CC
BanII 454 GAGCCC G[GA]GC[CT]^C
BclI 1056 TGATCA T^GATCA
BfaI 614 CTAG C^TAG
BglI 697 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 454 GAGCCC G[AGT]GC[ACT]^C
BsaI 593 GGTCTC GGTCTC(1/5)
BsgI 401 CTGCAC GTGCAG(16/14)
Bsp1286I 454 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 838 TGTACA T^GTACA
BspLU11I 292 ACATGT A^CATGT
BsrDI 323 CATTGC GCAATG(2/0)
BsrGI 838 TGTACA T^GTACA
BsrI 450 ACTGG ACTGG(1/-1)
BstXI 255 CCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 134 GCATGC GC[ACGT]^[ACGT]GC
DpnII 1057 GATC ^GATC
DraI 514 TTTAAA TTT^AAA
DraII 27 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 552 CCATGG C^C[GA][CT]GG
EcoNI 355 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 27 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 552 CCATGG C^C[AT][AT]GG
FbaI 1056 TGATCA T^GATCA
HphI 463 TCACC GGTGA(8/7)
MaeI 614 CTAG C^TAG
MboI 1057 GATC ^GATC
MfeI 272 CAATTG C^AATTG
MspA1I 707 CAGCTG C[AC]G^C[GT]G
MunI 272 CAATTG C^AATTG
NcoI 552 CCATGG C^CATGG
NdeII 1057 GATC ^GATC
NlaIV 453 GGAGCC GG[ACGT]^[ACGT]CC
PflMI 108 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 707 CAGCTG CAG^CTG
Sau3AI 1057 GATC ^GATC
SexAI 535 ACCAGGT A^CC[AT]GGT
SfcI 582 CTATAG C^T[GA][CT]AG
SphI 134 GCATGC GCATG^C
SspBI 838 TGTACA T^GTACA
StuI 625 AGGCCT AGG^CCT
StyI 552 CCATGG C^C[AT][AT]GG
Van91I 108 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 903 CCATTACCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BpmI 263 CTGGAG CTGGAG(16/14)
451 CTGGAG
BsaJI 552 CCATGG C^C[ACGT][ACGT]GG
1038 CCGAGG
BssSI 671 CACGAG CACGAG(-5/-1)
953 CACGAG
BstF5I 545 CATCC GGATG(2/0)
871 GGATG
CfoI 42 GCGC GCG^C
121 GCGC
Cfr13I 28 GGGCC G^G[ACGT]CC
539 GGTCC
DdeI 9 CTCAG C^T[ACGT]AG
1073 CTCAG
FokI 545 CATCC GGATG(9/13)
871 GGATG
HhaI 42 GCGC GCG^C
121 GCGC
HinP1I 42 GCGC G^CGC
121 GCGC
MwoI 44 GCAAATGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
697 GCCAAGAAGGC
Sau96I 28 GGGCC G^G[ACGT]CC
539 GGTCC
TspRI 316 CACTG CA[GC]TG[ACGT][ACGT]^
910 CACTG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
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AcsI AfaI AluI ApoI BbvI
BsiYI BslI BsmAI BssKI BstNI
EcoRII Fnu4HI HaeIII ItaI MaeIII
MboII MnlI MseI MvaI NlaIII
NspI RsaI ScrFI Tru9I TseI
Tsp45I Tsp509I