(Circular) (Six-base) MAPSORT of: pFN21ASDA1809.seq Check: 8294 from: 1 to: 7048 pFN21ASDA1809 7048 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3660 4858 278 Size: 53 83 186 936 2124 1198 2468 Fragments arranged by size: 2468 2124 1198 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4391 1937 Size: 2454 4594 Acc65I G'GTAC_C Cuts at: 4369 4369 Size: 7048 AclI AA'CG_TT Cuts at: 1563 5175 5548 1563 Size: 3612 373 3063 Fragments arranged by size: 3612 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2649 3302 3679 5111 6495 6763 1632 Size: 1017 653 377 1432 1384 268 1917 Fragments arranged by size: 1917 1432 1384 1017 653 377 268 AflII C'TTAA_G Cuts at: 820 1017 2056 4334 820 Size: 197 1039 2278 3534 Fragments arranged by size: 3534 2278 1039 197 AflIII A'CryG_T Cuts at: 1203 2534 2997 3258 3984 5947 6924 1203 Size: 1331 463 261 726 1963 977 1327 Fragments arranged by size: 1963 1331 1327 977 726 463 261 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 5777 5777 Size: 7048 AleI CACnn'nnGTG Cuts at: 1166 2904 1166 Size: 1738 5310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7048 AlwNI CAG_nnn'CTG Cuts at: 2247 3718 4791 6363 2247 Size: 1471 1073 1572 2932 Fragments arranged by size: 2932 1572 1471 1073 ApaI G_GGCC'C Cuts at: 4211 4211 Size: 7048 ApaLI G'TGCA_C Cuts at: 3203 3262 5105 6261 3203 Size: 59 1843 1156 3990 Fragments arranged by size: 3990 1843 1156 59 ApoI r'AATT_y Cuts at: 1493 2069 2379 3182 4357 4583 4735 6844 1493 Size: 576 310 803 1175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 803 576 310 226 152 AscI GG'CGCG_CC Cuts at: 3587 3587 Size: 7048 AseI AT'TA_AT Cuts at: 160 1032 5602 160 Size: 872 4570 1606 Fragments arranged by size: 4570 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2902 3847 3862 3935 4130 4153 Size: 73 135 824 945 15 73 195 23 Cuts at: 4153 4210 4324 4373 1870 Size: 57 114 49 4545 Fragments arranged by size: 4545 945 824 195 135 114 73 73 57 49 23 15 BamHI G'GATC_C Cuts at: 4378 4745 4378 Size: 367 6681 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3374 4369 5824 618 Size: 325 217 627 1587 995 1455 1842 Fragments arranged by size: 1842 1587 1455 995 627 325 217 BanII G_rGCy'C Cuts at: 1391 2137 2461 2523 3914 4211 4294 4333 4367 Size: 746 324 62 1391 297 83 39 34 Cuts at: 4367 1391 Size: 4072 Fragments arranged by size: 4072 1391 746 324 297 83 62 39 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3302 3789 928 Size: 971 1403 487 4187 Fragments arranged by size: 4187 1403 971 487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 5239 5273 1903 Size: 34 1979 34 1289 34 3678 Fragments arranged by size: 3678 1979 1289 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2669 4940 6150 2669 Size: 2271 1210 3567 Fragments arranged by size: 3567 2271 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7048 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3222 5659 136 Size: 107 122 71 631 605 1550 2437 1525 Fragments arranged by size: 2437 1550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7043 7043 Size: 7048 BlpI GC'TnA_GC Cuts at: 3572 3677 3908 4465 3572 Size: 105 231 557 6155 Fragments arranged by size: 6155 557 231 105 Bme1580I G_kGCm'C Cuts at: 1790 3207 3266 4211 5109 6265 1790 Size: 1417 59 945 898 1156 2573 Fragments arranged by size: 2573 1417 1156 945 898 59 BmgBI CAC'GTC Cuts at: 1318 3000 1318 Size: 1682 5366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4779 5737 442 Size: 416 53 83 399 24 3362 958 1753 Fragments arranged by size: 3362 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3622 3877 4111 3622 Size: 255 234 6559 Fragments arranged by size: 6559 255 234 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 2443 3599 4003 4042 5708 1239 Size: 882 322 1156 404 39 1666 2579 Fragments arranged by size: 2579 1666 1156 882 404 322 39 Bpu10I CC'TnA_GC Cuts at: 4043 4043 Size: 7048 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3395 4278 5173 6038 6336 3395 Size: 883 895 865 298 4107 Fragments arranged by size: 4107 895 883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3350 5711 882 Size: 617 1851 2361 2219 Fragments arranged by size: 2361 2219 1851 617 BsaAI yAC'GTr Cuts at: 493 1204 4345 4839 493 Size: 711 3141 494 2702 Fragments arranged by size: 3141 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2761 7042 2761 Size: 4281 2767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3092 3657 4855 5237 Size: 53 83 186 936 1559 565 1198 382 Cuts at: 5237 5872 275 Size: 635 1451 Fragments arranged by size: 1559 1451 1198 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5481 5879 6153 6300 6711 5481 Size: 398 274 147 411 5818 Fragments arranged by size: 5818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3332 3362 3971 4001 4031 Size: 30 165 30 1929 30 609 30 30 Cuts at: 4031 1178 Size: 4195 Fragments arranged by size: 4195 1929 609 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2425 3096 3114 3491 3500 3527 3536 Size: 961 278 671 18 377 9 27 9 Cuts at: 3536 3539 3542 3545 4069 4084 1186 Size: 3 3 3 524 15 4150 Fragments arranged by size: 4150 961 671 524 377 278 27 18 15 9 9 3 3 3 BseYI C'CCAG_C Cuts at: 2049 2522 2691 3029 3132 3889 3899 4220 6251 Size: 473 169 338 103 757 10 321 2031 Cuts at: 6251 6747 2049 Size: 496 2350 Fragments arranged by size: 2350 2031 757 496 473 338 321 169 103 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2433 2505 2697 1163 Size: 573 666 31 72 192 5514 Fragments arranged by size: 5514 666 573 192 72 31 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3621 3963 4498 5259 6287 1540 Size: 275 186 1620 342 535 761 1028 2301 Fragments arranged by size: 2301 1620 1028 761 535 342 275 186 BsiHKAI G_wGCw'C Cuts at: 2137 2220 2461 3207 3266 3678 4092 4187 4294 Size: 83 241 746 59 412 414 95 107 Cuts at: 4294 5109 5194 6265 2137 Size: 815 85 1071 2920 Fragments arranged by size: 2920 1071 815 746 414 412 241 107 95 85 83 59 BsmI GAATG_Cn' Cuts at: 4559 4652 4559 Size: 93 6955 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2220 2461 2523 3207 3266 3678 Size: 399 347 83 241 62 684 59 412 Cuts at: 3678 3914 4092 4187 4211 4294 4333 4367 5109 Size: 236 178 95 24 83 39 34 742 Cuts at: 5109 5194 6265 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 684 412 399 347 241 236 178 95 85 83 83 62 59 39 34 24 BspEI T'CCGG_A Cuts at: 6711 6711 Size: 7048 BspHI T'CATG_A Cuts at: 2149 4937 6667 2149 Size: 2788 1730 2530 Fragments arranged by size: 2788 2530 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2748 3960 4403 844 Size: 1904 1212 443 3489 Fragments arranged by size: 3489 1904 1212 443 BsrBI CCG'CTC Cuts at: 1394 1823 4935 1394 Size: 429 3112 3507 Fragments arranged by size: 3507 3112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2242 5543 5717 65 Size: 1183 994 3301 174 1396 Fragments arranged by size: 3301 1396 1183 994 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3024 3207 3794 4004 5692 5879 1700 Size: 110 1214 183 587 210 1688 187 2869 Fragments arranged by size: 2869 1688 1214 587 210 187 183 110 BsrGI T'GTAC_A Cuts at: 96 2537 96 Size: 2441 4607 BssHII G'CGCG_C Cuts at: 1925 3405 3587 1925 Size: 1480 182 5386 Fragments arranged by size: 5386 1480 182 BssSI C'ACGA_G Cuts at: 3596 5108 6120 3596 Size: 1512 1012 4524 Fragments arranged by size: 4524 1512 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2247 2492 3249 794 Size: 1453 245 757 4593 Fragments arranged by size: 4593 1453 757 245 BstBI TT'CG_AA Cuts at: 2007 5852 2007 Size: 3845 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7048 BstXI CCAn_nnnn'nTGG Cuts at: 2317 3385 4199 2317 Size: 1068 814 5166 Fragments arranged by size: 5166 1068 814 BstYI r'GATC_y Cuts at: 1972 4033 4149 4378 4745 5130 5147 6599 6685 Size: 2061 116 229 367 385 17 1452 86 Cuts at: 6685 6697 7043 1972 Size: 12 346 1977 Fragments arranged by size: 2061 1977 1452 385 367 346 229 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2119 2316 2648 2704 2958 3417 3835 Size: 552 1054 197 332 56 254 459 418 Cuts at: 3835 513 Size: 3726 Fragments arranged by size: 3726 1054 552 459 418 332 254 197 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3980 487 Size: 683 56 2754 3555 Fragments arranged by size: 3555 2754 683 56 BtsI GCAGTG_nn' Cuts at: 2177 4001 4564 5358 5378 2177 Size: 1824 563 794 20 3847 Fragments arranged by size: 3847 1824 794 563 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4283 4318 529 Size: 35 3719 35 3259 Fragments arranged by size: 3719 3259 35 35 DraI TTT'AAA Cuts at: 4352 4352 Size: 7048 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3331 6055 809 Size: 507 2015 2724 1802 Fragments arranged by size: 2724 2015 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2889 3423 3739 Size: 54 1425 50 275 18 1059 534 316 Cuts at: 3739 3747 3792 3832 3960 4495 5384 7013 8 Size: 8 45 40 128 535 889 1629 43 Fragments arranged by size: 1629 1425 1059 889 535 534 316 275 128 54 50 45 43 40 18 8 EagI C'GGCC_G Cuts at: 1537 1812 3960 4495 1537 Size: 275 2148 535 4090 Fragments arranged by size: 4090 2148 535 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 3477 3495 3504 3513 4978 1353 Size: 1518 606 18 9 9 1465 3423 Fragments arranged by size: 3423 1518 1465 606 18 9 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2888 3106 5635 6009 6155 387 Size: 1064 138 1299 218 2529 374 146 1280 Fragments arranged by size: 2529 1299 1280 1064 374 218 146 138 EcoICRI GAG'CTC Cuts at: 2135 2459 4292 2135 Size: 324 1833 4891 Fragments arranged by size: 4891 1833 324 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2443 2649 3302 3599 3679 4003 Size: 393 489 322 206 653 297 80 324 Cuts at: 4003 4042 5111 5708 6495 6763 1239 Size: 39 1069 597 787 268 1524 Fragments arranged by size: 1524 1069 787 653 597 489 393 324 322 297 268 206 80 39 EcoNI CCTnn'n_nnAGG Cuts at: 2914 3749 2914 Size: 835 6213 EcoO109I rG'GnC_Cy Cuts at: 2428 3715 3868 2428 Size: 1287 153 5608 Fragments arranged by size: 5608 1287 153 EcoRI G'AATT_C Cuts at: 2069 4357 4735 2069 Size: 2288 378 4382 Fragments arranged by size: 4382 2288 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2114 3097 2114 Size: 983 6065 FseI GG_CCGG'CC Cuts at: 3028 3028 Size: 7048 FspI TGC'GCA Cuts at: 5554 5554 Size: 7048 HaeII r_GCGC'y Cuts at: 1678 2797 2866 3289 3399 6195 1678 Size: 1119 69 423 110 2796 2531 Fragments arranged by size: 2796 2531 1119 423 110 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2448 2480 2595 Size: 32 135 32 773 32 71 32 115 Cuts at: 2595 2627 3332 3364 3770 3802 4307 4339 5689 Size: 32 705 32 406 32 505 32 1350 Cuts at: 5689 5721 5763 5795 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 773 705 505 406 135 115 71 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4392 4638 669 Size: 988 281 2454 246 3079 Fragments arranged by size: 3079 2454 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7048 HpaI GTT'AAC Cuts at: 4638 4638 Size: 7048 KpnI G_GTAC'C Cuts at: 4373 4373 Size: 7048 MfeI C'AATT_G Cuts at: 4647 4647 Size: 7048 MluI A'CGCG_T Cuts at: 3258 6924 3258 Size: 3666 3382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3360 3783 4065 6162 6346 1305 Size: 2055 423 282 2097 184 2007 Fragments arranged by size: 2097 2055 2007 423 282 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2891 3425 10 Size: 54 1425 343 1059 534 3633 Fragments arranged by size: 3633 1425 1059 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2904 4197 5006 5365 5524 518 Size: 648 1738 1293 809 359 159 2042 Fragments arranged by size: 2042 1738 1293 809 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2650 3013 3837 4164 4464 5141 6289 6534 Size: 1044 363 824 327 300 677 1148 245 Cuts at: 6534 1606 Size: 2120 Fragments arranged by size: 2120 1148 1044 824 677 363 327 300 245 NaeI GCC'GGC Cuts at: 3026 3796 4006 3026 Size: 770 210 6068 Fragments arranged by size: 6068 770 210 NcoI C'CATG_G Cuts at: 513 1065 2704 2958 513 Size: 552 1639 254 4603 Fragments arranged by size: 4603 1639 552 254 NdeI CA'TA_TG Cuts at: 387 387 Size: 7048 NgoMIV G'CCGG_C Cuts at: 3024 3794 4004 3024 Size: 770 210 6068 Fragments arranged by size: 6068 770 210 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 4495 4495 Size: 7048 NspI r_CATG'y Cuts at: 1132 1229 2538 3307 4406 5951 1132 Size: 97 1309 769 1099 1545 2229 Fragments arranged by size: 2229 1545 1309 1099 769 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7048 PciI A'CATG_T Cuts at: 2534 5947 2534 Size: 3413 3635 PflMI CCAn_nnn'nTGG Cuts at: 1108 2568 3718 1108 Size: 1460 1150 4438 Fragments arranged by size: 4438 1460 1150 PfoI T'CCnGG_A Cuts at: 3459 3459 Size: 7048 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 3929 3961 3974 4006 5117 5149 6630 Size: 32 1886 32 13 32 1111 32 1481 Cuts at: 6630 6662 2011 Size: 32 2397 Fragments arranged by size: 2397 1886 1481 1111 32 32 32 32 32 13 PpuMI rG'GwC_Cy Cuts at: 3715 3715 Size: 7048 PshAI GACnn'nnGTC Cuts at: 1613 3342 1613 Size: 1729 5319 PsiI TTA'TAA Cuts at: 4618 4618 Size: 7048 PspOMI G'GGCC_C Cuts at: 4207 4207 Size: 7048 PstI C_TGCA'G Cuts at: 830 4400 830 Size: 3570 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 RsrII CG'GwC_CG Cuts at: 3805 3805 Size: 7048 SacI G_AGCT'C Cuts at: 2137 2461 4294 2137 Size: 324 1833 4891 Fragments arranged by size: 4891 1833 324 SacII CC_GC'GG Cuts at: 2651 3838 2651 Size: 1187 5861 SalI G'TCGA_C Cuts at: 1936 4390 1936 Size: 2454 4594 SbfI CC_TGCA'GG Cuts at: 4400 4400 Size: 7048 ScaI AGT'ACT Cuts at: 5296 7008 5296 Size: 1712 5336 SexAI A'CCwGG_T Cuts at: 3195 3711 3195 Size: 516 6532 SfcI C'TryA_G Cuts at: 826 1045 4396 5531 6212 6403 826 Size: 219 3351 1135 681 191 1471 Fragments arranged by size: 3351 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 3207 1700 Size: 1507 5541 SmaI CCC'GGG Cuts at: 1872 3849 4132 4212 4375 1872 Size: 1977 283 80 163 4545 Fragments arranged by size: 4545 1977 283 163 80 SmlI C'TyrA_G Cuts at: 820 1017 1943 2056 3410 3935 4293 4324 4334 Size: 197 926 113 1354 525 358 31 10 Cuts at: 4334 5152 6053 6315 820 Size: 818 901 262 1553 Fragments arranged by size: 1553 1354 926 901 818 525 358 262 197 113 31 10 SnaBI TAC'GTA Cuts at: 493 4345 4839 493 Size: 3852 494 2702 Fragments arranged by size: 3852 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7048 SphI G_CATG'C Cuts at: 1132 4406 1132 Size: 3274 3774 SrfI GCCC'GGGC Cuts at: 4212 4212 Size: 7048 SspI AAT'ATT Cuts at: 5 52 4972 5 Size: 47 4920 2081 Fragments arranged by size: 4920 2081 47 StuI AGG'CCT Cuts at: 1517 3704 1517 Size: 2187 4861 StyI C'CwwG_G Cuts at: 513 1065 2704 2958 4487 513 Size: 552 1639 254 1529 3074 Fragments arranged by size: 3074 1639 1529 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2818 2909 3281 5075 5092 5245 5430 Size: 1255 1238 91 372 1794 17 153 185 Cuts at: 5430 5875 325 Size: 445 1498 Fragments arranged by size: 1794 1498 1255 1238 445 372 185 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2537 3987 5294 Size: 274 80 33 51 1443 560 1450 1307 Cuts at: 5294 7006 96 Size: 1712 138 Fragments arranged by size: 1712 1450 1443 1307 560 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3299 3331 7008 7040 3299 Size: 32 3677 32 3307 Fragments arranged by size: 3677 3307 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7048 XbaI T'CTAG_A Cuts at: 2372 4384 2372 Size: 2012 5036 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3385 3385 Size: 7048 XhoI C'TCGA_G Cuts at: 1943 3935 4324 1943 Size: 1992 389 4667 Fragments arranged by size: 4667 1992 389 XmaI C'CCGG_G Cuts at: 1870 3847 4130 4210 4373 1870 Size: 1977 283 80 163 4545 Fragments arranged by size: 4545 1977 283 163 80 XmnI GAAnn'nnTTC Cuts at: 2094 3944 5177 2094 Size: 1850 1233 3965 Fragments arranged by size: 3965 1850 1233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3658 4856 276 Size: 53 83 186 936 2124 1198 2468 Fragments arranged by size: 2468 2124 1198 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII SanDI SapI SfiI SfoI SwaI