Restriction map for
pFN21ASDA1802
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrBI BssHII BssSI BstAPI BstBI
BstUI CfoI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NarI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 100 GGTACC G^GTACC
AccI 229 GTATAC GT^[AC][GT]AC
AocI 1325 CCTCAGG CC^T[ACGT]AGG
Asp718I 100 GGTACC G^GTACC
AvaI 1594 CCCGAG C^[CT]CG[GA]G
BanI 100 GGTACC G^G[CT][GA]CC
BglII 1338 AGATCT A^GATCT
BlpI 1885 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1885 GCTGAGC GC^T[ACGT]AGC
BsmI 7 GCATTC GAATGC(1/-1)
BsoBI 1594 CCCGAG C^[CT]CG[GA]G
Bsp1407I 84 TGTACA T^GTACA
BspDI 1561 ATCGAT AT^CGAT
BsrDI 52 CATTGC GCAATG(2/0)
BsrFI 2366 GCCGGC [GA]^CCGG[CT]
BsrGI 84 TGTACA T^GTACA
Bst1107I 229 GTATAC GTA^TAC
BstEII 1331 GGTCACC G^GT[ACGT]ACC
BstPI 1331 GGTCACC G^GT[ACGT]ACC
BstZ17I 229 GTATAC GTA^TAC
Bsu36I 1325 CCTCAGG CC^T[ACGT]AGG
CelII 1885 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2366 GCCGGC [GA]^CCGG[CT]
ClaI 1561 ATCGAT AT^CGAT
Eco81I 1325 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1331 GGTCACC G^GT[ACGT]ACC
EcoRV 1537 GATATC GAT^ATC
EcoT22I 254 ATGCAT ATGCA^T
FauI 913 CCCGC CCCGC(4/6)
HgaI 1882 GACGC GACGC(5/10)
KpnI 100 GGTACC GGTAC^C
NaeI 2366 GCCGGC GCC^GGC
NcoI 2277 CCATGG C^CATGG
NdeI 94 CATATG CA^TATG
NgoMIV 2366 GCCGGC G^CCGGC
NsiI 254 ATGCAT ATGCA^T
PflMI 1130 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 782 CAGCTG CAG^CTG
SexAI 1494 ACCAGGT A^CC[AT]GGT
SmlI 1855 CTCAAG C^T[CT][GA]AG
SpeI 2020 ACTAGT A^CTAGT
SphI 1668 GCATGC GCATG^C
SspBI 84 TGTACA T^GTACA
StuI 961 AGGCCT AGG^CCT
TaqI 1562 TCGA T^CGA
TthHB8I 1562 TCGA T^CGA
Van91I 1130 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2035 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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Asp700I 870 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1792 GAAGAACTTC
AspHI 1312 GAGCTC G[AT]GC[AT]^C
1875 GAGCTC
AvrII 898 CCTAGG C^CTAGG
958 CCTAGG
BlnI 898 CCTAGG C^CTAGG
958 CCTAGG
BsaI 903 GAGACC GGTCTC(1/5)
1483 GAGACC
BsiHKAI 1312 GAGCTC G[AT]GC[AT]^C
1875 GAGCTC
BsiYI 387 CCTTTCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1130 CCAGCTCCTGG
BslI 387 CCTTTCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1130 CCAGCTCCTGG
DsaI 1416 CCGTGG C^C[GA][CT]GG
2277 CCATGG
HindIII 1348 AAGCTT A^AGCTT
2069 AAGCTT
NspI 252 ACATGC [GA]CATG^[CT]
1668 GCATGC
SacI 1312 GAGCTC GAGCT^C
1875 GAGCTC
SfcI 499 CTACAG C^T[GA][CT]AG
2047 CTACAG
XmnI 870 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1792 GAAGAACTTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII