(Circular) (Six-base) MAPSORT of: pFN21ASDA1802.seq Check: 4129 from: 1 to: 7165 pFN21ASDA1802 7165 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4975 278 Size: 53 83 186 936 3439 2468 Fragments arranged by size: 3439 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2253 4508 1937 Size: 316 2255 4594 Fragments arranged by size: 4594 2255 316 Acc65I G'GTAC_C Cuts at: 2123 4486 2123 Size: 2363 4802 AclI AA'CG_TT Cuts at: 1563 5292 5665 1563 Size: 3729 373 3063 Fragments arranged by size: 3729 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2727 2856 2988 4101 4125 5228 6612 6880 Size: 1095 129 132 1113 24 1103 1384 268 Cuts at: 6880 1632 Size: 1917 Fragments arranged by size: 1917 1384 1113 1103 1095 268 132 129 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6968 AflIII A'CryG_T Cuts at: 1203 6064 7041 1203 Size: 4861 977 1327 Fragments arranged by size: 4861 1327 977 AgeI A'CCGG_T Cuts at: 5996 5996 Size: 7165 AhdI GACnn_n'nnGTC Cuts at: 5894 5894 Size: 7165 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7165 AlwNI CAG_nnn'CTG Cuts at: 2336 2597 3159 3908 4908 6480 2336 Size: 261 562 749 1000 1572 3021 Fragments arranged by size: 3021 1572 1000 749 562 261 ApaLI G'TGCA_C Cuts at: 5222 6378 5222 Size: 1156 6009 ApoI r'AATT_y Cuts at: 1493 2138 4158 4345 4455 4474 4700 4852 6961 Size: 645 2020 187 110 19 226 152 2109 Cuts at: 6961 1493 Size: 1697 Fragments arranged by size: 2109 2020 1697 645 226 187 152 110 19 AseI AT'TA_AT Cuts at: 160 1032 5719 160 Size: 872 4687 1606 Fragments arranged by size: 4687 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7165 AvaI C'yCGr_G Cuts at: 1870 1943 3617 4490 1870 Size: 73 1674 873 4545 Fragments arranged by size: 4545 1674 873 73 AvrII C'CTAG_G Cuts at: 2921 2981 2921 Size: 60 7105 BamHI G'GATC_C Cuts at: 4495 4862 4495 Size: 367 6798 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 4486 5941 618 Size: 325 217 627 336 2363 1455 1842 Fragments arranged by size: 2363 1842 1455 627 336 325 217 BanII G_rGCy'C Cuts at: 1391 3339 3684 3902 4484 1391 Size: 1948 345 218 582 4072 Fragments arranged by size: 4072 1948 582 345 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 3459 3490 928 Size: 971 1212 348 31 4603 Fragments arranged by size: 4603 1212 971 348 31 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5356 5390 1903 Size: 34 3419 34 3678 Fragments arranged by size: 3678 3419 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5057 6267 5057 Size: 1210 5955 BclI T'GATC_A Cuts at: 1548 3382 3787 3982 1548 Size: 1834 405 195 4731 Fragments arranged by size: 4731 1834 405 195 BfrBI ATG'CAT Cuts at: 2279 2279 Size: 7165 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5776 136 Size: 107 122 71 631 605 4104 1525 Fragments arranged by size: 4104 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3361 7160 3361 Size: 3799 3366 BlpI GC'TnA_GC Cuts at: 3909 4582 3909 Size: 673 6492 Bme1580I G_kGCm'C Cuts at: 1790 3670 5226 6382 1790 Size: 1880 1556 1156 2573 Fragments arranged by size: 2573 1880 1556 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3257 4896 5854 Size: 416 53 83 399 24 1840 1639 958 Cuts at: 5854 442 Size: 1753 Fragments arranged by size: 1840 1753 1639 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7165 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2433 2504 2619 2739 2799 3255 3315 3633 Size: 1194 71 115 120 60 456 60 318 Cuts at: 3633 4463 5825 1239 Size: 830 1362 2579 Fragments arranged by size: 2579 1362 1194 830 456 318 120 115 71 60 60 Bpu10I CC'TnA_GC Cuts at: 2517 2544 2850 2517 Size: 27 306 6832 Fragments arranged by size: 6832 306 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3863 5290 6155 6453 3863 Size: 1427 865 298 4575 Fragments arranged by size: 4575 1427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2920 3500 5828 882 Size: 617 1421 580 2328 2219 Fragments arranged by size: 2328 2219 1421 617 580 BsaAI yAC'GTr Cuts at: 493 1204 4462 4956 493 Size: 711 3258 494 2702 Fragments arranged by size: 3258 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7159 7159 Size: 7165 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4972 5354 5989 275 Size: 53 83 186 936 3439 382 635 1451 Fragments arranged by size: 3439 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5598 5996 6270 6417 6828 5598 Size: 398 274 147 411 5935 Fragments arranged by size: 5935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3195 3220 3225 3250 3622 Size: 30 165 30 1792 25 5 25 372 Cuts at: 3622 3652 1178 Size: 30 4691 Fragments arranged by size: 4691 1792 372 165 30 30 30 25 25 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2340 2460 4124 4460 1186 Size: 1154 120 1664 336 3891 Fragments arranged by size: 3891 1664 1154 336 120 BseYI C'CCAG_C Cuts at: 2803 3152 6368 6864 2803 Size: 349 3216 496 3104 Fragments arranged by size: 3216 3104 496 349 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6592 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4615 5376 6404 1540 Size: 275 186 2614 761 1028 2301 Fragments arranged by size: 2614 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3339 3902 5226 5311 6382 3339 Size: 563 1324 85 1071 4122 Fragments arranged by size: 4122 1324 1071 563 85 BsmI GAATG_Cn' Cuts at: 2030 4676 4769 2030 Size: 2646 93 4426 Fragments arranged by size: 4426 2646 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3339 3670 3684 3902 4484 5226 5311 Size: 399 1549 331 14 218 582 742 85 Cuts at: 5311 6382 1391 Size: 1071 2174 Fragments arranged by size: 2174 1549 1071 742 582 399 331 218 85 14 BspEI T'CCGG_A Cuts at: 6828 6828 Size: 7165 BspHI T'CATG_A Cuts at: 5054 6784 5054 Size: 1730 5435 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2413 2566 2659 3046 3613 3982 4520 844 Size: 1569 153 93 387 567 369 538 3489 Fragments arranged by size: 3489 1569 567 538 387 369 153 93 BsrBI CCG'CTC Cuts at: 1394 1823 5052 1394 Size: 429 3229 3507 Fragments arranged by size: 3507 3229 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2074 5660 5834 65 Size: 1183 826 3586 174 1396 Fragments arranged by size: 3586 1396 1183 826 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4389 5809 5996 1700 Size: 110 2579 1420 187 2869 Fragments arranged by size: 2869 2579 1420 187 110 BsrGI T'GTAC_A Cuts at: 96 2107 96 Size: 2011 5154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7165 BssSI C'ACGA_G Cuts at: 5225 6237 5225 Size: 1012 6153 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7165 BstBI TT'CG_AA Cuts at: 2007 5969 2007 Size: 3962 3203 BstEII G'GTnAC_C Cuts at: 1184 3354 1184 Size: 2170 4995 BstXI CCAn_nnnn'nTGG Cuts at: 2292 2790 2955 3366 2292 Size: 498 165 411 6091 Fragments arranged by size: 6091 498 411 165 BstYI r'GATC_y Cuts at: 1972 2332 3309 3326 3361 4495 4862 5247 5264 Size: 360 977 17 35 1134 367 385 17 Cuts at: 5264 6716 6802 6814 7160 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1134 977 385 367 360 346 86 35 17 17 12 BstZ17I GTA'TAC Cuts at: 2254 2254 Size: 7165 Bsu36I CC'TnA_GG Cuts at: 3349 3349 Size: 7165 BtgI C'CryG_G Cuts at: 513 1065 2022 3439 4300 513 Size: 552 957 1417 861 3378 Fragments arranged by size: 3378 1417 957 861 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3965 487 Size: 683 56 2739 3687 Fragments arranged by size: 3687 2739 683 56 BtsI GCAGTG_nn' Cuts at: 2209 3985 4681 5475 5495 2209 Size: 1776 696 794 20 3879 Fragments arranged by size: 3879 1776 794 696 20 ClaI AT'CG_AT Cuts at: 1554 3585 1554 Size: 2031 5134 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7130 DraI TTT'AAA Cuts at: 4469 4469 Size: 7165 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6172 809 Size: 507 4856 1802 Fragments arranged by size: 4856 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4612 5501 7130 Size: 54 1425 50 275 18 2782 889 1629 Cuts at: 7130 8 Size: 43 Fragments arranged by size: 2782 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4612 1537 Size: 275 2800 4090 Fragments arranged by size: 4090 2800 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 2538 4101 4395 5095 1353 Size: 1005 180 1563 294 700 3423 Fragments arranged by size: 3423 1563 1005 700 294 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5752 6126 6272 387 Size: 1064 138 4163 374 146 1280 Fragments arranged by size: 4163 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3337 3900 3337 Size: 563 6602 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 2504 2619 2727 2739 2799 2856 Size: 393 801 71 115 108 12 60 57 Cuts at: 2856 2988 3255 3315 3633 4101 4125 4463 5228 Size: 132 267 60 318 468 24 338 765 Cuts at: 5228 5825 6612 6880 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 801 787 765 597 468 393 338 318 268 267 132 115 108 71 60 60 57 24 12 EcoO109I rG'GnC_Cy Cuts at: 2976 3017 3521 2976 Size: 41 504 6620 Fragments arranged by size: 6620 504 41 EcoRI G'AATT_C Cuts at: 4474 4852 4474 Size: 378 6787 EcoRV GAT'ATC Cuts at: 3562 3562 Size: 7165 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3487 3859 3487 Size: 372 6793 FspI TGC'GCA Cuts at: 5671 5671 Size: 7165 HaeII r_GCGC'y Cuts at: 1678 6312 1678 Size: 4634 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3301 3333 3989 4021 5806 Size: 32 135 32 1729 32 656 32 1785 Cuts at: 5806 5838 5880 5912 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1785 1729 656 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4509 4755 669 Size: 988 281 2571 246 3079 Fragments arranged by size: 3079 2571 988 281 246 HindIII A'AGCT_T Cuts at: 748 3371 4092 748 Size: 2623 721 3821 Fragments arranged by size: 3821 2623 721 HpaI GTT'AAC Cuts at: 4755 4755 Size: 7165 KpnI G_GTAC'C Cuts at: 2127 4490 2127 Size: 2363 4802 MfeI C'AATT_G Cuts at: 4764 4764 Size: 7165 MluI A'CGCG_T Cuts at: 7041 7041 Size: 7165 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6279 6463 1305 Size: 4974 184 2007 Fragments arranged by size: 4974 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5343 Fragments arranged by size: 5343 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5123 5482 5641 518 Size: 648 3957 359 159 2042 Fragments arranged by size: 3957 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2807 2939 3388 4581 5258 6406 6651 1606 Size: 1201 132 449 1193 677 1148 245 2120 Fragments arranged by size: 2120 1201 1193 1148 677 449 245 132 NaeI GCC'GGC Cuts at: 4391 4391 Size: 7165 NcoI C'CATG_G Cuts at: 513 1065 2022 4300 513 Size: 552 957 2278 3378 Fragments arranged by size: 3378 2278 957 552 NdeI CA'TA_TG Cuts at: 387 2118 387 Size: 1731 5434 NgoMIV G'CCGG_C Cuts at: 4389 4389 Size: 7165 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7165 NotI GC'GGCC_GC Cuts at: 4612 4612 Size: 7165 NsiI A_TGCA'T Cuts at: 2281 2281 Size: 7165 NspI r_CATG'y Cuts at: 1132 1229 2279 3695 4523 6068 1132 Size: 97 1050 1416 828 1545 2229 Fragments arranged by size: 2229 1545 1416 1050 828 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7165 PciI A'CATG_T Cuts at: 6064 6064 Size: 7165 PflMI CCAn_nnn'nTGG Cuts at: 1108 3159 1108 Size: 2051 5114 PfoI T'CCnGG_A Cuts at: 2034 2721 3055 3088 3128 3158 2034 Size: 687 334 33 40 30 6041 Fragments arranged by size: 6041 687 334 40 33 30 PmeI GTTT'AAAC Cuts at: 4469 4469 Size: 7165 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5234 5266 6747 6779 5234 Size: 32 1481 32 5620 Fragments arranged by size: 5620 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2976 3017 3521 2976 Size: 41 504 6620 Fragments arranged by size: 6620 504 41 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7165 PsiI TTA'TAA Cuts at: 4735 4735 Size: 7165 PstI C_TGCA'G Cuts at: 830 4517 830 Size: 3687 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7165 PvuII CAG'CTG Cuts at: 2807 2807 Size: 7165 SacI G_AGCT'C Cuts at: 3339 3902 3339 Size: 563 6602 SalI G'TCGA_C Cuts at: 1936 4507 1936 Size: 2571 4594 SanDI GG'GwC_CC Cuts at: 2976 2976 Size: 7165 SbfI CC_TGCA'GG Cuts at: 4517 4517 Size: 7165 ScaI AGT'ACT Cuts at: 5413 7125 5413 Size: 1712 5453 SexAI A'CCwGG_T Cuts at: 3517 3517 Size: 7165 SfcI C'TryA_G Cuts at: 826 1045 2522 4070 4513 5648 6329 6520 826 Size: 219 1477 1548 443 1135 681 191 1471 Fragments arranged by size: 1548 1477 1471 1135 681 443 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7165 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7165 SmaI CCC'GGG Cuts at: 1872 4492 1872 Size: 2620 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3878 5269 6170 6432 820 Size: 197 926 1935 1391 901 262 1553 Fragments arranged by size: 1935 1553 1391 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4462 4956 493 Size: 3969 494 2702 Fragments arranged by size: 3969 2702 494 SpeI A'CTAG_T Cuts at: 152 4043 152 Size: 3891 3274 SphI G_CATG'C Cuts at: 1132 3695 4523 1132 Size: 2563 828 3774 Fragments arranged by size: 3774 2563 828 SspI AAT'ATT Cuts at: 5 52 5089 5 Size: 47 5037 2081 Fragments arranged by size: 5037 2081 47 StuI AGG'CCT Cuts at: 1517 2986 1517 Size: 1469 5696 StyI C'CwwG_G Cuts at: 513 1065 2022 2789 2855 2888 2921 2981 2987 Size: 552 957 767 66 33 33 60 6 Cuts at: 2987 3020 3062 3095 4300 4604 513 Size: 33 42 33 1205 304 3074 Fragments arranged by size: 3074 1205 957 767 552 304 66 60 42 33 33 33 33 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3166 5192 5209 5362 5547 5992 325 Size: 1255 1586 2026 17 153 185 445 1498 Fragments arranged by size: 2026 1586 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2107 2241 3927 Size: 274 80 33 51 1443 130 134 1686 Cuts at: 3927 5411 7123 96 Size: 1484 1712 138 Fragments arranged by size: 1712 1686 1484 1443 274 138 134 130 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 3195 3220 3227 3252 3399 3431 7125 Size: 32 911 25 7 25 147 32 3694 Cuts at: 7125 7157 2252 Size: 32 2260 Fragments arranged by size: 3694 2260 911 147 32 32 32 25 25 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7165 XbaI T'CTAG_A Cuts at: 4058 4501 4058 Size: 443 6722 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7165 XmaI C'CCGG_G Cuts at: 1870 4490 1870 Size: 2620 4545 XmnI GAAnn'nnTTC Cuts at: 2897 3819 5294 2897 Size: 922 1475 4768 Fragments arranged by size: 4768 1475 922 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4973 276 Size: 53 83 186 936 3439 2468 Fragments arranged by size: 3439 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI DraIII EcoNI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI