Restriction map for
pFN21ASDA1802
(Linear) (Six-base) MAPSORT of: KIAA1802.seq Check: 1927 from: 1 to: 2436
KIAA1802.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 230 2436
Size: 230 2206
Acc65I G'GTAC_C
Cuts at: 0 100 2436
Size: 100 2336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 833 965 2078 2102 2436
Size: 704 129 132 1113 24 334
Fragments arranged by size:
1113 704 334 132 129 24
AlwNI CAG_nnn'CTG
Cuts at: 0 313 574 1136 1885 2436
Size: 313 261 562 749 551
Fragments arranged by size:
749 562 551 313 261
ApoI r'AATT_y
Cuts at: 0 115 2135 2322 2436
Size: 115 2020 187 114
Fragments arranged by size:
2020 187 115 114
AvaI C'yCGr_G
Cuts at: 0 1594 2436
Size: 1594 842
AvrII C'CTAG_G
Cuts at: 0 898 958 2436
Size: 898 60 1478
Fragments arranged by size:
1478 898 60
BanI G'GyrC_C
Cuts at: 0 100 2436
Size: 100 2336
BanII G_rGCy'C
Cuts at: 0 1316 1661 1879 2436
Size: 1316 345 218 557
Fragments arranged by size:
1316 557 345 218
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 1436 1467 2436
Size: 1088 348 31 969
Fragments arranged by size:
1088 969 348 31
BclI T'GATC_A
Cuts at: 0 1359 1764 1959 2436
Size: 1359 405 195 477
Fragments arranged by size:
1359 477 405 195
BfrBI ATG'CAT
Cuts at: 0 256 2436
Size: 256 2180
BglII A'GATC_T
Cuts at: 0 1338 2436
Size: 1338 1098
BlpI GC'TnA_GC
Cuts at: 0 1886 2436
Size: 1886 550
Bme1580I G_kGCm'C
Cuts at: 0 1647 2436
Size: 1647 789
BmrI ACTGGGnnnn_n'
Cuts at: 0 1234 2436
Size: 1234 1202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 481 596 716 776 1232 1292 1610
Size: 410 71 115 120 60 456 60 318
Cuts at: 1610 2436
Size: 826
Fragments arranged by size:
826 456 410 318 120 115 71 60
60
Bpu10I CC'TnA_GC
Cuts at: 0 494 521 827 2436
Size: 494 27 306 1609
Fragments arranged by size:
1609 494 306 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1840 2436
Size: 1840 596
BsaI GGTCTCn'nnnn_
Cuts at: 0 897 1477 2436
Size: 897 580 959
Fragments arranged by size:
959 897 580
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1172 1197 1202 1227 1599 1629 2436
Size: 1172 25 5 25 372 30 807
Fragments arranged by size:
1172 807 372 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 317 437 2101 2436
Size: 317 120 1664 335
Fragments arranged by size:
1664 335 317 120
BseYI C'CCAG_C
Cuts at: 0 780 1129 2436
Size: 780 349 1307
Fragments arranged by size:
1307 780 349
BsiHKAI G_wGCw'C
Cuts at: 0 1316 1879 2436
Size: 1316 563 557
Fragments arranged by size:
1316 563 557
BsmI GAATG_Cn'
Cuts at: 0 7 2436
Size: 7 2429
Bsp1286I G_dGCh'C
Cuts at: 0 1316 1647 1661 1879 2436
Size: 1316 331 14 218 557
Fragments arranged by size:
1316 557 331 218 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 543 636 1023 1590 1959 2436
Size: 390 153 93 387 567 369 477
Fragments arranged by size:
567 477 390 387 369 153 93
BsrDI GCAATG_nn'
Cuts at: 0 51 2436
Size: 51 2385
BsrFI r'CCGG_y
Cuts at: 0 2366 2436
Size: 2366 70
BsrGI T'GTAC_A
Cuts at: 0 84 2436
Size: 84 2352
BstEII G'GTnAC_C
Cuts at: 0 1331 2436
Size: 1331 1105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 269 767 932 1343 2436
Size: 269 498 165 411 1093
Fragments arranged by size:
1093 498 411 269 165
BstYI r'GATC_y
Cuts at: 0 309 1286 1303 1338 2436
Size: 309 977 17 35 1098
Fragments arranged by size:
1098 977 309 35 17
BstZ17I GTA'TAC
Cuts at: 0 231 2436
Size: 231 2205
Bsu36I CC'TnA_GG
Cuts at: 0 1326 2436
Size: 1326 1110
BtgI C'CryG_G
Cuts at: 0 1416 2277 2436
Size: 1416 861 159
Fragments arranged by size:
1416 861 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1942 2436
Size: 1942 494
BtsI GCAGTG_nn'
Cuts at: 0 186 1962 2436
Size: 186 1776 474
Fragments arranged by size:
1776 474 186
ClaI AT'CG_AT
Cuts at: 0 1562 2436
Size: 1562 874
EarI CTCTTCn'nnn_
Cuts at: 0 335 515 2078 2372 2436
Size: 335 180 1563 294 64
Fragments arranged by size:
1563 335 294 180 64
EcoICRI GAG'CTC
Cuts at: 0 1314 1877 2436
Size: 1314 563 559
Fragments arranged by size:
1314 563 559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 481 596 704 716 776 833 965
Size: 410 71 115 108 12 60 57 132
Cuts at: 965 1232 1292 1610 2078 2102 2436
Size: 267 60 318 468 24 334
Fragments arranged by size:
468 410 334 318 267 132 115 108
71 60 60 57 24 12
EcoO109I rG'GnC_Cy
Cuts at: 0 953 994 1498 2436
Size: 953 41 504 938
Fragments arranged by size:
953 938 504 41
EcoRV GAT'ATC
Cuts at: 0 1539 2436
Size: 1539 897
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1464 1836 2436
Size: 1464 372 600
Fragments arranged by size:
1464 600 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1278 1310 1966 1998 2436
Size: 1278 32 656 32 438
Fragments arranged by size:
1278 656 438 32 32
HindIII A'AGCT_T
Cuts at: 0 1348 2069 2436
Size: 1348 721 367
Fragments arranged by size:
1348 721 367
KpnI G_GTAC'C
Cuts at: 0 104 2436
Size: 104 2332
MspA1I CmG'CkG
Cuts at: 0 784 916 1365 2436
Size: 784 132 449 1071
Fragments arranged by size:
1071 784 449 132
NaeI GCC'GGC
Cuts at: 0 2368 2436
Size: 2368 68
NcoI C'CATG_G
Cuts at: 0 2277 2436
Size: 2277 159
NdeI CA'TA_TG
Cuts at: 0 95 2436
Size: 95 2341
NgoMIV G'CCGG_C
Cuts at: 0 2366 2436
Size: 2366 70
NsiI A_TGCA'T
Cuts at: 0 258 2436
Size: 258 2178
NspI r_CATG'y
Cuts at: 0 256 1672 2436
Size: 256 1416 764
Fragments arranged by size:
1416 764 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 1136 2436
Size: 1136 1300
PfoI T'CCnGG_A
Cuts at: 0 11 698 1032 1065 1105 1135 2436
Size: 11 687 334 33 40 30 1301
Fragments arranged by size:
1301 687 334 40 33 30 11
PpuMI rG'GwC_Cy
Cuts at: 0 953 994 1498 2436
Size: 953 41 504 938
Fragments arranged by size:
953 938 504 41
PvuII CAG'CTG
Cuts at: 0 784 2436
Size: 784 1652
SacI G_AGCT'C
Cuts at: 0 1316 1879 2436
Size: 1316 563 557
Fragments arranged by size:
1316 563 557
SanDI GG'GwC_CC
Cuts at: 0 953 2436
Size: 953 1483
SexAI A'CCwGG_T
Cuts at: 0 1494 2436
Size: 1494 942
SfcI C'TryA_G
Cuts at: 0 499 2047 2436
Size: 499 1548 389
Fragments arranged by size:
1548 499 389
SmlI C'TyrA_G
Cuts at: 0 1855 2436
Size: 1855 581
SpeI A'CTAG_T
Cuts at: 0 2020 2436
Size: 2020 416
SphI G_CATG'C
Cuts at: 0 1672 2436
Size: 1672 764
StuI AGG'CCT
Cuts at: 0 963 2436
Size: 963 1473
StyI C'CwwG_G
Cuts at: 0 766 832 865 898 958 964 997 1039
Size: 766 66 33 33 60 6 33 42
Cuts at: 1039 1072 2277 2436
Size: 33 1205 159
Fragments arranged by size:
1205 766 159 66 60 42 33 33
33 33 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1143 2436
Size: 1143 1293
TatI w'GTAC_w
Cuts at: 0 84 218 1904 2436
Size: 84 134 1686 532
Fragments arranged by size:
1686 532 134 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 1172 1197 1204 1229 1376 1408
Size: 229 32 911 25 7 25 147 32
Cuts at: 1408 2436
Size: 1028
Fragments arranged by size:
1028 911 229 147 32 32 25 25
7
XbaI T'CTAG_A
Cuts at: 0 2035 2436
Size: 2035 401
XmnI GAAnn'nnTTC
Cuts at: 0 874 1796 2436
Size: 874 922 640
Fragments arranged by size:
922 874 640
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApoI AvaI AvrII BanI
BanII BbsI BclI BfrBI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI EarI
EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HindIII KpnI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PflMI
PfoI PpuMI PvuII SacI SanDI SexAI SfcI SmlI
SpeI SphI StuI StyI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BcgI BciVI BglI BmgBI
BmtI BplI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI
BstBI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoNI EcoRI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MmeI MscI MslI NarI NheI
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SspI SwaI Tth111I XcmI
XhoI XmaI ZraI