(Circular) (Six-base) MAPSORT of: pFN21ASDA1798.seq Check: 7437 from: 1 to: 7069 pFN21ASDA1798 7069 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 2284 Size: 7069 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4879 278 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3162 3474 4412 1937 Size: 1225 312 938 4594 Fragments arranged by size: 4594 1225 938 312 Acc65I G'GTAC_C Cuts at: 2218 4390 2218 Size: 2172 4897 AclI AA'CG_TT Cuts at: 1563 5196 5569 1563 Size: 3633 373 3063 Fragments arranged by size: 3633 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 3626 3663 5132 6516 6784 1632 Size: 725 1269 37 1469 1384 268 1917 Fragments arranged by size: 1917 1469 1384 1269 725 268 37 AflII C'TTAA_G Cuts at: 820 1017 3059 3820 820 Size: 197 2042 761 4069 Fragments arranged by size: 4069 2042 761 197 AflIII A'CryG_T Cuts at: 1203 3065 5968 6945 1203 Size: 1862 2903 977 1327 Fragments arranged by size: 2903 1862 1327 977 AgeI A'CCGG_T Cuts at: 5900 5900 Size: 7069 AhdI GACnn_n'nnGTC Cuts at: 4159 5798 4159 Size: 1639 5430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7069 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3993 4025 3993 Size: 32 7037 AlwNI CAG_nnn'CTG Cuts at: 4812 6384 4812 Size: 1572 5497 ApaI G_GGCC'C Cuts at: 3752 4295 3752 Size: 543 6526 ApaLI G'TGCA_C Cuts at: 5126 6282 5126 Size: 1156 5913 ApoI r'AATT_y Cuts at: 1493 3038 4166 4305 4345 4378 4604 4756 6865 Size: 1545 1128 139 40 33 226 152 2109 Cuts at: 6865 1493 Size: 1697 Fragments arranged by size: 2109 1697 1545 1128 226 152 139 40 33 AseI AT'TA_AT Cuts at: 160 1032 3668 5623 160 Size: 872 2636 1955 1606 Fragments arranged by size: 2636 1955 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 AvaI C'yCGr_G Cuts at: 1870 1943 2251 2846 3703 3993 4394 1870 Size: 73 308 595 857 290 401 4545 Fragments arranged by size: 4545 857 595 401 308 290 73 BamHI G'GATC_C Cuts at: 2350 2842 4399 4766 2350 Size: 492 1557 367 4653 Fragments arranged by size: 4653 1557 492 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 3989 4390 5845 618 Size: 325 217 627 431 1771 401 1455 1842 Fragments arranged by size: 1842 1771 1455 627 431 401 325 217 BanII G_rGCy'C Cuts at: 1391 2149 2258 3659 3752 4295 4388 1391 Size: 758 109 1401 93 543 93 4072 Fragments arranged by size: 4072 1401 758 543 109 93 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2330 2508 3942 928 Size: 971 431 178 1434 4055 Fragments arranged by size: 4055 1434 971 431 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5260 5294 1903 Size: 34 3323 34 3678 Fragments arranged by size: 3678 3323 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2888 4961 6171 2888 Size: 2073 1210 3786 Fragments arranged by size: 3786 2073 1210 BclI T'GATC_A Cuts at: 1548 3526 1548 Size: 1978 5091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5680 136 Size: 107 122 71 631 605 4008 1525 Fragments arranged by size: 4008 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7064 7064 Size: 7069 BlpI GC'TnA_GC Cuts at: 4486 4486 Size: 7069 Bme1580I G_kGCm'C Cuts at: 1790 2102 3752 3994 4295 5130 6286 1790 Size: 312 1650 242 301 835 1156 2573 Fragments arranged by size: 2573 1650 1156 835 312 301 242 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7069 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2337 2969 3253 Size: 416 53 83 399 24 920 632 284 Cuts at: 3253 4298 4800 5758 442 Size: 1045 502 958 1753 Fragments arranged by size: 1753 1045 958 920 632 502 416 399 284 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7069 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2242 2445 5729 1239 Size: 1003 203 3284 2579 Fragments arranged by size: 3284 2579 1003 203 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2214 3060 3173 3485 5194 6059 6357 2214 Size: 846 113 312 1709 865 298 2926 Fragments arranged by size: 2926 1709 865 846 312 298 113 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3903 5732 882 Size: 617 2404 1829 2219 Fragments arranged by size: 2404 2219 1829 617 BsaAI yAC'GTr Cuts at: 493 1204 4366 4860 493 Size: 711 3162 494 2702 Fragments arranged by size: 3162 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2472 7063 2472 Size: 4591 2478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4876 5258 5893 275 Size: 53 83 186 936 3343 382 635 1451 Fragments arranged by size: 3343 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2898 5502 5900 6174 6321 6732 2898 Size: 2604 398 274 147 411 3235 Fragments arranged by size: 3235 2604 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6844 Fragments arranged by size: 6844 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2756 3426 1186 Size: 1570 670 4829 Fragments arranged by size: 4829 1570 670 BseYI C'CCAG_C Cuts at: 2721 4080 6272 6768 2721 Size: 1359 2192 496 3022 Fragments arranged by size: 3022 2192 1359 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4519 5280 6308 1540 Size: 275 186 2518 761 1028 2301 Fragments arranged by size: 2518 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2258 2455 2650 4151 5130 5215 6286 2258 Size: 197 195 1501 979 85 1071 3041 Fragments arranged by size: 3041 1501 1071 979 197 195 85 BsmI GAATG_Cn' Cuts at: 2956 4580 4673 2956 Size: 1624 93 5352 Fragments arranged by size: 5352 1624 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2149 2258 2455 2650 3659 3752 Size: 399 312 47 109 197 195 1009 93 Cuts at: 3752 3994 4151 4295 4388 5130 5215 6286 1391 Size: 242 157 144 93 742 85 1071 2174 Fragments arranged by size: 2174 1071 1009 742 399 312 242 197 195 157 144 109 93 93 85 47 BspEI T'CCGG_A Cuts at: 6732 6732 Size: 7069 BspHI T'CATG_A Cuts at: 4958 6688 4958 Size: 1730 5339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2284 3412 4424 844 Size: 1440 1128 1012 3489 Fragments arranged by size: 3489 1440 1128 1012 BsrBI CCG'CTC Cuts at: 1394 1823 4956 1394 Size: 429 3133 3507 Fragments arranged by size: 3507 3133 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5564 5738 65 Size: 1183 4316 174 1396 Fragments arranged by size: 4316 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2761 2996 5713 5900 1700 Size: 110 951 235 2717 187 2869 Fragments arranged by size: 2869 2717 951 235 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7069 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7069 BssSI C'ACGA_G Cuts at: 5129 6141 5129 Size: 1012 6057 BstAPI GCAn_nnn'nTGC Cuts at: 794 2692 794 Size: 1898 5171 BstBI TT'CG_AA Cuts at: 2007 5873 2007 Size: 3866 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7069 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2978 2982 3303 2215 Size: 763 4 321 5981 Fragments arranged by size: 5981 763 321 4 BstYI r'GATC_y Cuts at: 1972 2350 2518 2842 4399 4766 5151 5168 6620 Size: 378 168 324 1557 367 385 17 1452 Cuts at: 6620 6706 6718 7064 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1557 1452 385 378 367 346 324 168 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2630 2630 Size: 7069 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6517 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6330 Fragments arranged by size: 6330 683 56 BtsI GCAGTG_nn' Cuts at: 2763 4585 5379 5399 2763 Size: 1822 794 20 4433 Fragments arranged by size: 4433 1822 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7034 DraI TTT'AAA Cuts at: 4213 4373 4213 Size: 160 6909 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6076 809 Size: 507 4760 1802 Fragments arranged by size: 4760 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2998 3721 4516 Size: 54 1425 50 275 18 1168 723 795 Cuts at: 4516 5405 7034 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1168 889 795 723 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4516 1537 Size: 275 2704 4090 Fragments arranged by size: 4090 2704 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2580 2733 3895 3939 4999 1353 Size: 1227 153 1162 44 1060 3423 Fragments arranged by size: 3423 1227 1162 1060 153 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5656 6030 6176 387 Size: 1064 138 4067 374 146 1280 Fragments arranged by size: 4067 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2256 2256 Size: 7069 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2242 2357 2445 3626 3663 5132 5729 Size: 393 610 115 88 1181 37 1469 597 Cuts at: 5729 6516 6784 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1469 1181 787 610 597 393 268 115 88 37 EcoNI CCTnn'n_nnAGG Cuts at: 2700 2738 3018 2700 Size: 38 280 6751 Fragments arranged by size: 6751 280 38 EcoO109I rG'GnC_Cy Cuts at: 3749 4292 3749 Size: 543 6526 EcoRI G'AATT_C Cuts at: 3038 4345 4378 4756 3038 Size: 1307 33 378 5351 Fragments arranged by size: 5351 1307 378 33 EcoRV GAT'ATC Cuts at: 2968 2968 Size: 7069 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2929 3909 2929 Size: 980 6089 FspI TGC'GCA Cuts at: 5575 5575 Size: 7069 HaeII r_GCGC'y Cuts at: 1678 6216 1678 Size: 4538 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2771 2803 2840 2872 4145 Size: 32 135 32 1199 32 37 32 1273 Cuts at: 4145 4177 5710 5742 5784 5816 1373 Size: 32 1533 32 42 32 2626 Fragments arranged by size: 2626 1533 1273 1199 135 42 37 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4413 4659 669 Size: 988 281 2475 246 3079 Fragments arranged by size: 3079 2475 988 281 246 HindIII A'AGCT_T Cuts at: 748 2906 3149 3461 3675 748 Size: 2158 243 312 214 4142 Fragments arranged by size: 4142 2158 312 243 214 HpaI GTT'AAC Cuts at: 4659 4659 Size: 7069 KpnI G_GTAC'C Cuts at: 2222 4394 2222 Size: 2172 4897 MfeI C'AATT_G Cuts at: 3043 3562 4668 3043 Size: 519 1106 5444 Fragments arranged by size: 5444 1106 519 MluI A'CGCG_T Cuts at: 6945 6945 Size: 7069 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2513 4130 6183 6367 1305 Size: 1208 1617 2053 184 2007 Fragments arranged by size: 2053 2007 1617 1208 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3723 10 Size: 54 1425 343 1891 3356 Fragments arranged by size: 3356 1891 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2299 2916 3549 3921 4322 5027 Size: 648 1047 86 617 633 372 401 705 Cuts at: 5027 5386 5545 518 Size: 359 159 2042 Fragments arranged by size: 2042 1047 705 648 633 617 401 372 359 159 86 MspA1I CmG'CkG Cuts at: 1606 4034 4485 5162 6310 6555 1606 Size: 2428 451 677 1148 245 2120 Fragments arranged by size: 2428 2120 1148 677 451 245 NaeI GCC'GGC Cuts at: 2763 2763 Size: 7069 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6517 NdeI CA'TA_TG Cuts at: 387 387 Size: 7069 NgoMIV G'CCGG_C Cuts at: 2761 2761 Size: 7069 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7069 NotI GC'GGCC_GC Cuts at: 4516 4516 Size: 7069 NspI r_CATG'y Cuts at: 1132 1229 3069 3920 4427 5972 1132 Size: 97 1840 851 507 1545 2229 Fragments arranged by size: 2229 1840 1545 851 507 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7069 PciI A'CATG_T Cuts at: 3065 5968 3065 Size: 2903 4166 PflMI CCAn_nnn'nTGG Cuts at: 1108 2211 1108 Size: 1103 5966 PmeI GTTT'AAAC Cuts at: 4373 4373 Size: 7069 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5138 5170 6651 6683 5138 Size: 32 1481 32 5524 Fragments arranged by size: 5524 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7069 PsiI TTA'TAA Cuts at: 4639 4639 Size: 7069 PspOMI G'GGCC_C Cuts at: 3748 4291 3748 Size: 543 6526 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3316 3348 3316 Size: 32 7037 PstI C_TGCA'G Cuts at: 830 4339 4421 830 Size: 3509 82 3478 Fragments arranged by size: 3509 3478 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7069 PvuII CAG'CTG Cuts at: 4034 4034 Size: 7069 SacI G_AGCT'C Cuts at: 2258 2258 Size: 7069 SalI G'TCGA_C Cuts at: 1936 4411 1936 Size: 2475 4594 SapI GCTCTTCn'nnn_ Cuts at: 3895 3895 Size: 7069 SbfI CC_TGCA'GG Cuts at: 4421 4421 Size: 7069 ScaI AGT'ACT Cuts at: 5317 7029 5317 Size: 1712 5357 SexAI A'CCwGG_T Cuts at: 3340 3340 Size: 7069 SfcI C'TryA_G Cuts at: 826 1045 3609 4335 4417 5552 6233 6424 826 Size: 219 2564 726 82 1135 681 191 1471 Fragments arranged by size: 2564 1471 1135 726 681 219 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7069 SmaI CCC'GGG Cuts at: 1872 3705 3995 4396 1872 Size: 1833 290 401 4545 Fragments arranged by size: 4545 1833 401 290 SmlI C'TyrA_G Cuts at: 820 1017 1943 2229 3059 3075 3152 3464 3820 Size: 197 926 286 830 16 77 312 356 Cuts at: 3820 5173 6074 6336 820 Size: 1353 901 262 1553 Fragments arranged by size: 1553 1353 926 901 830 356 312 286 262 197 77 16 SnaBI TAC'GTA Cuts at: 493 4366 4860 493 Size: 3873 494 2702 Fragments arranged by size: 3873 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7069 SphI G_CATG'C Cuts at: 1132 3920 4427 1132 Size: 2788 507 3774 Fragments arranged by size: 3774 2788 507 SspI AAT'ATT Cuts at: 5 52 4993 5 Size: 47 4941 2081 Fragments arranged by size: 4941 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7069 StyI C'CwwG_G Cuts at: 513 1065 4508 513 Size: 552 3443 3074 Fragments arranged by size: 3443 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2935 2970 2988 4017 4176 5096 5113 Size: 1255 1355 35 18 1029 159 920 17 Cuts at: 5113 5266 5451 5896 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1355 1255 1029 920 445 185 159 153 35 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2677 2862 5315 Size: 274 80 33 51 1443 700 185 2453 Cuts at: 5315 7027 96 Size: 1712 138 Fragments arranged by size: 2453 1712 1443 700 274 185 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7029 7061 7029 Size: 32 7037 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7069 XbaI T'CTAG_A Cuts at: 4405 4405 Size: 7069 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 2280 2212 Size: 68 7001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7069 XmaI C'CCGG_G Cuts at: 1870 3703 3993 4394 1870 Size: 1833 290 401 4545 Fragments arranged by size: 4545 1833 401 290 XmnI GAAnn'nnTTC Cuts at: 2370 2771 4239 5198 2370 Size: 401 1468 959 4241 Fragments arranged by size: 4241 1468 959 401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4877 276 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI