Restriction map for
pFN21ASDA1792
(Circular) (Six-base) MAPSORT of: pFN21ASDA1792.seq Check: 3590 from: 1 to: 7021
pFN21ASDA1792 7021 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3444 3452 3444
Size: 8 7013
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4831 278
Size: 53 83 186 936 3295 2468
Fragments arranged by size:
3295 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4364 1937
Size: 2427 4594
Acc65I G'GTAC_C
Cuts at: 2978 4342 2978
Size: 1364 5657
AclI AA'CG_TT
Cuts at: 1563 5148 5521 1563
Size: 3585 373 3063
Fragments arranged by size:
3585 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3617 3836 5084 6468 6736 1632
Size: 1985 219 1248 1384 268 1917
Fragments arranged by size:
1985 1917 1384 1248 268 219
AflII C'TTAA_G
Cuts at: 820 1017 3554 820
Size: 197 2537 4287
Fragments arranged by size:
4287 2537 197
AflIII A'CryG_T
Cuts at: 1203 5920 6897 1203
Size: 4717 977 1327
Fragments arranged by size:
4717 1327 977
AgeI A'CCGG_T
Cuts at: 5852 5852
Size: 7021
AhdI GACnn_n'nnGTC
Cuts at: 5750 5750
Size: 7021
AleI CACnn'nnGTG
Cuts at: 1166 3122 1166
Size: 1956 5065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7021
AlwNI CAG_nnn'CTG
Cuts at: 2437 3073 3266 3971 4764 6336 2437
Size: 636 193 705 793 1572 3122
Fragments arranged by size:
3122 1572 793 705 636 193
ApaI G_GGCC'C
Cuts at: 3741 3741
Size: 7021
ApaLI G'TGCA_C
Cuts at: 5078 6234 5078
Size: 1156 5865
ApoI r'AATT_y
Cuts at: 1493 2258 3023 3174 3368 3413 3682 3695 4330
Size: 765 765 151 194 45 269 13 635
Cuts at: 4330 4556 4708 6817 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 765 635 269 226 194
152 151 45 13
AseI AT'TA_AT
Cuts at: 160 1032 5575 160
Size: 872 4543 1606
Fragments arranged by size:
4543 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
AvaI C'yCGr_G
Cuts at: 1870 1943 4346 1870
Size: 73 2403 4545
Fragments arranged by size:
4545 2403 73
AvrII C'CTAG_G
Cuts at: 2798 2798
Size: 7021
BamHI G'GATC_C
Cuts at: 4351 4718 4351
Size: 367 6654
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2978 4342 5797 618
Size: 325 217 627 1191 1364 1455 1842
Fragments arranged by size:
1842 1455 1364 1191 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2306 2537 3741 4340 1391
Size: 915 231 1204 599 4072
Fragments arranged by size:
4072 1204 915 599 231
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6050
BbvCI CC'TCA_GC
Cuts at: 3168 3168
Size: 7021
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2052 2086 4181 4215 5212 5246 1903
Size: 34 115 34 2095 34 997 34 3678
Fragments arranged by size:
3678 2095 997 115 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2914 4913 6123 2914
Size: 1999 1210 3812
Fragments arranged by size:
3812 1999 1210
BclI T'GATC_A
Cuts at: 1548 2640 2958 1548
Size: 1092 318 5611
Fragments arranged by size:
5611 1092 318
BfrBI ATG'CAT
Cuts at: 2417 2417
Size: 7021
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3453 3848 5632
Size: 107 122 71 631 605 1781 395 1784
Cuts at: 5632 136
Size: 1525
Fragments arranged by size:
1784 1781 1525 631 605 395 122 107
71
BglII A'GATC_T
Cuts at: 2635 7016 2635
Size: 4381 2640
BlpI GC'TnA_GC
Cuts at: 2531 4438 2531
Size: 1907 5114
Bme1580I G_kGCm'C
Cuts at: 1790 2245 3741 4079 5082 6238 1790
Size: 455 1496 338 1003 1156 2573
Fragments arranged by size:
2573 1496 1156 1003 455 338
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3736 4243 4752
Size: 416 53 83 399 24 2319 507 509
Cuts at: 4752 5710 442
Size: 958 1753
Fragments arranged by size:
2319 1753 958 509 507 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2480 2794 3361 3740 4138 5681 1239
Size: 897 344 314 567 379 398 1543 2579
Fragments arranged by size:
2579 1543 897 567 398 379 344 314
Bpu10I CC'TnA_GC
Cuts at: 3168 3528 3168
Size: 360 6661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2487 3896 5146 6011 6309 2021
Size: 466 1409 1250 865 298 2733
Fragments arranged by size:
2733 1409 1250 865 466 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2702 3716 5684 882
Size: 617 1203 1014 1968 2219
Fragments arranged by size:
2219 1968 1203 1014 617
BsaAI yAC'GTr
Cuts at: 493 1204 3124 4318 4812 493
Size: 711 1920 1194 494 2702
Fragments arranged by size:
2702 1920 1194 711 494
BsaBI GATnn'nnATC
Cuts at: 7015 7015
Size: 7021
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4828 5210 5845 275
Size: 53 83 186 936 3295 382 635 1451
Fragments arranged by size:
3295 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5454 5852 6126 6273 6684 5454
Size: 398 274 147 411 5791
Fragments arranged by size:
5791 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6796
Fragments arranged by size:
6796 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2984 3156 1186
Size: 1798 172 5051
Fragments arranged by size:
5051 1798 172
BseYI C'CCAG_C
Cuts at: 2299 2566 3451 3730 6224 6720 2299
Size: 267 885 279 2494 496 2600
Fragments arranged by size:
2600 2494 885 496 279 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 4157 1163
Size: 573 376 2045 4027
Fragments arranged by size:
4027 2045 573 376
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4471 5232 6260 1540
Size: 275 186 2470 761 1028 2301
Fragments arranged by size:
2470 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 5082 5167 6238 2537
Size: 2545 85 1071 3320
Fragments arranged by size:
3320 2545 1071 85
BsmI GAATG_Cn'
Cuts at: 3262 4532 4625 3262
Size: 1270 93 5658
Fragments arranged by size:
5658 1270 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2245 2306 2537 3741 4079 4340 5082
Size: 399 455 61 231 1204 338 261 742
Cuts at: 5082 5167 6238 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1204 1071 742 455 399 338 261
231 85 61
BspEI T'CCGG_A
Cuts at: 6684 6684
Size: 7021
BspHI T'CATG_A
Cuts at: 3141 4288 4910 6640 3141
Size: 1147 622 1730 3522
Fragments arranged by size:
3522 1730 1147 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3444 3452 4376 844
Size: 2600 8 924 3489
Fragments arranged by size:
3489 2600 924 8
BsrBI CCG'CTC
Cuts at: 1394 1823 4908 1394
Size: 429 3085 3507
Fragments arranged by size:
3507 3085 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2291 4039 5516 5690 65
Size: 1183 1043 1748 1477 174 1396
Fragments arranged by size:
1748 1477 1396 1183 1043 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3847 5665 5852 1700
Size: 110 2037 1818 187 2869
Fragments arranged by size:
2869 2037 1818 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7021
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7021
BssSI C'ACGA_G
Cuts at: 5081 6093 5081
Size: 1012 6009
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3266 794
Size: 2472 4549
BstBI TT'CG_AA
Cuts at: 2007 5825 2007
Size: 3818 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7021
BstXI CCAn_nnnn'nTGG
Cuts at: 2989 3991 4063 2989
Size: 1002 72 5947
Fragments arranged by size:
5947 1002 72
BstYI r'GATC_y
Cuts at: 1972 2635 2845 4159 4351 4718 5103 5120 6572
Size: 663 210 1314 192 367 385 17 1452
Cuts at: 6572 6658 6670 7016 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1314 663 385 367 346 210
192 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3984 4056 513
Size: 552 957 1962 72 3478
Fragments arranged by size:
3478 1962 957 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6282
Fragments arranged by size:
6282 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3267 4537 5331 5351 2245
Size: 1022 1270 794 20 3915
Fragments arranged by size:
3915 1270 1022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7021
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2305 2340 529
Size: 35 1741 35 5210
Fragments arranged by size:
5210 1741 35 35
DraI TTT'AAA
Cuts at: 2808 3001 4325 2808
Size: 193 1324 5504
Fragments arranged by size:
5504 1324 193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3794 6028 809
Size: 507 2478 2234 1802
Fragments arranged by size:
2478 2234 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4468 5357 6986
Size: 54 1425 50 275 18 2638 889 1629
Cuts at: 6986 8
Size: 43
Fragments arranged by size:
2638 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4468 1537
Size: 275 2656 4090
Fragments arranged by size:
4090 2656 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2218 2325 4178 4951 1353
Size: 865 107 1853 773 3423
Fragments arranged by size:
3423 1853 865 773 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5608 5982 6128 387
Size: 1064 138 4019 374 146 1280
Fragments arranged by size:
4019 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2535 2535
Size: 7021
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2480 2794 3361 3617 3740 3836
Size: 393 504 344 314 567 256 123 96
Cuts at: 3836 4138 5084 5681 6468 6736 1239
Size: 302 946 597 787 268 1524
Fragments arranged by size:
1524 946 787 597 567 504 393 344
314 302 268 256 123 96
EcoNI CCTnn'n_nnAGG
Cuts at: 3554 3554
Size: 7021
EcoO109I rG'GnC_Cy
Cuts at: 2146 2911 3737 3738 2146
Size: 765 826 1 5429
Fragments arranged by size:
5429 826 765 1
EcoRI G'AATT_C
Cuts at: 2258 3174 3413 3682 3695 4330 4708 2258
Size: 916 239 269 13 635 378 4571
Fragments arranged by size:
4571 916 635 378 269 239 13
FspI TGC'GCA
Cuts at: 5527 5527
Size: 7021
HaeII r_GCGC'y
Cuts at: 1678 6168 1678
Size: 4490 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3151 3183 3754 3786 5662
Size: 32 135 32 1579 32 571 32 1876
Cuts at: 5662 5694 5736 5768 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1876 1579 571 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3811 4365 4611 669
Size: 988 281 1873 554 246 3079
Fragments arranged by size:
3079 1873 988 554 281 246
HindIII A'AGCT_T
Cuts at: 748 2655 748
Size: 1907 5114
HpaI GTT'AAC
Cuts at: 3811 4611 3811
Size: 800 6221
KpnI G_GTAC'C
Cuts at: 2982 4346 2982
Size: 1364 5657
MfeI C'AATT_G
Cuts at: 4620 4620
Size: 7021
MluI A'CGCG_T
Cuts at: 6897 6897
Size: 7021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3972 6135 6319 1305
Size: 2667 2163 184 2007
Fragments arranged by size:
2667 2163 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5199
Fragments arranged by size:
5199 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3122 4061 4979 5338 5497 518
Size: 648 1956 939 918 359 159 2042
Fragments arranged by size:
2042 1956 939 918 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2599 4007 4195 4437 5114 6262 6507
Size: 693 300 1408 188 242 677 1148 245
Cuts at: 6507 1606
Size: 2120
Fragments arranged by size:
2120 1408 1148 693 677 300 245 242
188
NaeI GCC'GGC
Cuts at: 3849 3849
Size: 7021
NcoI C'CATG_G
Cuts at: 513 1065 2022 3984 4056 513
Size: 552 957 1962 72 3478
Fragments arranged by size:
3478 1962 957 552 72
NdeI CA'TA_TG
Cuts at: 387 2198 3759 387
Size: 1811 1561 3649
Fragments arranged by size:
3649 1811 1561
NgoMIV G'CCGG_C
Cuts at: 3847 3847
Size: 7021
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7021
NotI GC'GGCC_GC
Cuts at: 4468 4468
Size: 7021
NsiI A_TGCA'T
Cuts at: 2419 2419
Size: 7021
NspI r_CATG'y
Cuts at: 1132 1229 2368 3884 4093 4379 5924 1132
Size: 97 1139 1516 209 286 1545 2229
Fragments arranged by size:
2229 1545 1516 1139 286 209 97
PasI CC'CwG_GG
Cuts at: 1348 2310 3184 1348
Size: 962 874 5185
Fragments arranged by size:
5185 962 874
PciI A'CATG_T
Cuts at: 5920 5920
Size: 7021
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7021
PmeI GTTT'AAAC
Cuts at: 4325 4325
Size: 7021
PmlI CAC'GTG
Cuts at: 3124 3124
Size: 7021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5090 5122 6603 6635 5090
Size: 32 1481 32 5476
Fragments arranged by size:
5476 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2911 2146
Size: 765 6256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7021
PsiI TTA'TAA
Cuts at: 3348 4591 3348
Size: 1243 5778
PspOMI G'GGCC_C
Cuts at: 3737 3737
Size: 7021
PstI C_TGCA'G
Cuts at: 830 2548 4373 830
Size: 1718 1825 3478
Fragments arranged by size:
3478 1825 1718
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7021
PvuII CAG'CTG
Cuts at: 2299 2599 4007 4195 2299
Size: 300 1408 188 5125
Fragments arranged by size:
5125 1408 300 188
SacI G_AGCT'C
Cuts at: 2537 2537
Size: 7021
SalI G'TCGA_C
Cuts at: 1936 4363 1936
Size: 2427 4594
SbfI CC_TGCA'GG
Cuts at: 4373 4373
Size: 7021
ScaI AGT'ACT
Cuts at: 3010 3473 3625 5269 6981 3010
Size: 463 152 1644 1712 3050
Fragments arranged by size:
3050 1712 1644 463 152
SfcI C'TryA_G
Cuts at: 826 1045 2378 2544 4369 5504 6185 6376 826
Size: 219 1333 166 1825 1135 681 191 1471
Fragments arranged by size:
1825 1471 1333 1135 681 219 191 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7021
SmaI CCC'GGG
Cuts at: 1872 4348 1872
Size: 2476 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2502 3554 3875 5125 6026
Size: 197 926 93 466 1052 321 1250 901
Cuts at: 6026 6288 820
Size: 262 1553
Fragments arranged by size:
1553 1250 1052 926 901 466 321 262
197 93
SnaBI TAC'GTA
Cuts at: 493 4318 4812 493
Size: 3825 494 2702
Fragments arranged by size:
3825 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7021
SphI G_CATG'C
Cuts at: 1132 4093 4379 1132
Size: 2961 286 3774
Fragments arranged by size:
3774 2961 286
SspI AAT'ATT
Cuts at: 5 52 3097 4945 5
Size: 47 3045 1848 2081
Fragments arranged by size:
3045 2081 1848 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7021
StyI C'CwwG_G
Cuts at: 513 1065 2022 2798 3984 4056 4165 4460 513
Size: 552 957 776 1186 72 109 295 3074
Fragments arranged by size:
3074 1186 957 776 552 295 109 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2262 2323 3370 5048 5065 5218 5403
Size: 1255 682 61 1047 1678 17 153 185
Cuts at: 5403 5848 325
Size: 445 1498
Fragments arranged by size:
1678 1498 1255 1047 682 445 185 153
61 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2288 3008 3471
Size: 274 80 33 51 1443 311 720 463
Cuts at: 3471 3623 5267 6979 96
Size: 152 1644 1712 138
Fragments arranged by size:
1712 1644 1443 720 463 311 274 152
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6981 7013 6981
Size: 32 6989
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7021
XbaI T'CTAG_A
Cuts at: 4357 4357
Size: 7021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3448 4145 3448
Size: 697 6324
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7021
XmaI C'CCGG_G
Cuts at: 1870 4346 1870
Size: 2476 4545
XmnI GAAnn'nnTTC
Cuts at: 3178 4298 5150 3178
Size: 1120 852 5049
Fragments arranged by size:
5049 1120 852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4829 276
Size: 53 83 186 936 3295 2468
Fragments arranged by size:
3295 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI PacI PfoI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI