(Circular) (Six-base) MAPSORT of: pFN21ASDA1787.seq Check: 2700 from: 1 to: 9415 pFN21ASDA1787 9415 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5536 5615 5875 6016 5536 Size: 79 260 141 8935 Fragments arranged by size: 8935 260 141 79 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7225 278 Size: 53 83 186 936 5689 2468 Fragments arranged by size: 5689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6758 1937 Size: 4821 4594 Acc65I G'GTAC_C Cuts at: 6736 6736 Size: 9415 AclI AA'CG_TT Cuts at: 1563 7542 7915 1563 Size: 5979 373 3063 Fragments arranged by size: 5979 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 2638 2727 4401 4840 5766 6616 7478 Size: 779 227 89 1674 439 926 850 862 Cuts at: 7478 8862 9130 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1674 1384 926 862 850 779 439 268 227 89 AfeI AGC'GCT Cuts at: 2346 2346 Size: 9415 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9218 AflIII A'CryG_T Cuts at: 1203 2275 5223 5736 8314 9291 1203 Size: 1072 2948 513 2578 977 1327 Fragments arranged by size: 2948 2578 1327 1072 977 513 AgeI A'CCGG_T Cuts at: 8246 8246 Size: 9415 AhdI GACnn_n'nnGTC Cuts at: 4339 8144 4339 Size: 3805 5610 AleI CACnn'nnGTG Cuts at: 1166 4070 5480 1166 Size: 2904 1410 5101 Fragments arranged by size: 5101 2904 1410 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3716 1184 Size: 2532 6883 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2885 2917 2885 Size: 32 9383 AlwNI CAG_nnn'CTG Cuts at: 2470 2519 3575 4100 4751 5207 5539 5915 6344 Size: 49 1056 525 651 456 332 376 429 Cuts at: 6344 7158 8730 2470 Size: 814 1572 3155 Fragments arranged by size: 3155 1572 1056 814 651 525 456 429 376 332 49 ApaI G_GGCC'C Cuts at: 2096 3998 4288 5727 2096 Size: 1902 290 1439 5784 Fragments arranged by size: 5784 1902 1439 290 ApaLI G'TGCA_C Cuts at: 2617 4515 6417 7472 8628 2617 Size: 1898 1902 1055 1156 3404 Fragments arranged by size: 3404 1902 1898 1156 1055 ApoI r'AATT_y Cuts at: 1493 3056 3293 4391 6724 6950 7102 9211 1493 Size: 1563 237 1098 2333 226 152 2109 1697 Fragments arranged by size: 2333 2109 1697 1563 1098 237 226 152 AseI AT'TA_AT Cuts at: 160 1032 3372 7969 160 Size: 872 2340 4597 1606 Fragments arranged by size: 4597 2340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9415 AvaI C'yCGr_G Cuts at: 1870 1943 2687 3530 4018 4594 4976 5417 6183 Size: 73 744 843 488 576 382 441 766 Cuts at: 6183 6426 6586 6740 1870 Size: 243 160 154 4545 Fragments arranged by size: 4545 843 766 744 576 488 441 382 243 160 154 73 BamHI G'GATC_C Cuts at: 6745 7112 6745 Size: 367 9048 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3227 3272 5303 5527 5750 Size: 325 217 627 1440 45 2031 224 223 Cuts at: 5750 6736 8191 618 Size: 986 1455 1842 Fragments arranged by size: 2031 1842 1455 1440 986 627 325 224 223 217 45 BanII G_rGCy'C Cuts at: 1391 2054 2096 2402 2694 2883 2903 3700 3998 Size: 663 42 306 292 189 20 797 298 Cuts at: 3998 4166 4288 5025 5727 6734 1391 Size: 168 122 737 702 1007 4072 Fragments arranged by size: 4072 1007 797 737 702 663 306 298 292 189 168 122 42 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2272 2715 4389 4846 5039 5680 928 Size: 971 373 443 1674 457 193 641 4663 Fragments arranged by size: 4663 1674 971 641 457 443 373 193 BbvCI CC'TCA_GC Cuts at: 4871 4878 4871 Size: 7 9408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4804 4838 6023 6057 6099 6133 6398 Size: 34 2867 34 1185 34 42 34 265 Cuts at: 6398 6432 7606 7640 1903 Size: 34 1174 34 3678 Fragments arranged by size: 3678 2867 1185 1174 265 42 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5059 7307 8517 5059 Size: 2248 1210 5957 Fragments arranged by size: 5957 2248 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3566 5382 5620 Size: 107 122 71 631 605 1894 1816 238 Cuts at: 5620 5748 8026 136 Size: 128 2278 1525 Fragments arranged by size: 2278 1894 1816 1525 631 605 238 128 122 107 71 BglII A'GATC_T Cuts at: 5706 9410 5706 Size: 3704 5711 BlpI GC'TnA_GC Cuts at: 3033 3686 6832 3033 Size: 653 3146 5616 Fragments arranged by size: 5616 3146 653 Bme1580I G_kGCm'C Cuts at: 1790 2096 2621 3275 3903 3998 4017 4288 4511 Size: 306 525 654 628 95 19 271 223 Cuts at: 4511 4519 5532 5650 5727 5744 5841 6421 6691 Size: 8 1013 118 77 17 97 580 270 Cuts at: 6691 7476 8632 1790 Size: 785 1156 2573 Fragments arranged by size: 2573 1156 1013 785 654 628 580 525 306 271 270 223 118 97 95 77 19 17 8 BmgBI CAC'GTC Cuts at: 1318 4081 1318 Size: 2763 6652 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2175 2349 3407 Size: 416 53 83 399 24 758 174 1058 Cuts at: 3407 3861 4739 5316 7146 8104 442 Size: 454 878 577 1830 958 1753 Fragments arranged by size: 1830 1753 1058 958 878 758 577 454 416 399 174 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2335 3203 3785 4077 4380 5066 5309 5447 Size: 1096 868 582 292 303 686 243 138 Cuts at: 5447 6263 8075 1239 Size: 816 1812 2579 Fragments arranged by size: 2579 1812 1096 868 816 686 582 303 292 243 138 Bpu10I CC'TnA_GC Cuts at: 2110 2643 2897 3576 4871 4878 5904 6356 6543 Size: 533 254 679 1295 7 1026 452 187 Cuts at: 6543 2110 Size: 4982 Fragments arranged by size: 4982 1295 1026 679 533 452 254 187 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2822 3902 5687 6668 7540 8405 8703 2822 Size: 1080 1785 981 872 865 298 3534 Fragments arranged by size: 3534 1785 1080 981 872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5705 6479 8078 882 Size: 617 4206 774 1599 2219 Fragments arranged by size: 4206 2219 1599 774 617 BsaAI yAC'GTr Cuts at: 493 1204 5739 6712 7206 493 Size: 711 4535 973 494 2702 Fragments arranged by size: 4535 2702 973 711 494 BsaBI GATnn'nnATC Cuts at: 3119 5168 9409 3119 Size: 2049 4241 3125 Fragments arranged by size: 4241 3125 2049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3407 4023 4263 7222 Size: 53 83 186 936 1874 616 240 2959 Cuts at: 7222 7604 8239 275 Size: 382 635 1451 Fragments arranged by size: 2959 1874 1451 936 635 616 382 240 186 83 53 BsaWI w'CCGG_w Cuts at: 5025 7848 8246 8520 8667 9078 5025 Size: 2823 398 274 147 411 5362 Fragments arranged by size: 5362 2823 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2885 2915 5296 5326 1178 Size: 30 165 30 1482 30 2381 30 5267 Fragments arranged by size: 5267 2381 1482 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2468 4817 5306 5363 5381 6162 6413 6414 Size: 1282 2349 489 57 18 781 251 1 Cuts at: 6414 6446 6671 1186 Size: 32 225 3930 Fragments arranged by size: 3930 2349 1282 781 489 251 225 57 32 18 1 BseYI C'CCAG_C Cuts at: 2096 2519 2764 3199 4504 4939 5116 5307 5532 Size: 423 245 435 1305 435 177 191 225 Cuts at: 5532 5841 5994 6248 6331 6476 6525 8618 9114 Size: 309 153 254 83 145 49 2093 496 Cuts at: 9114 2096 Size: 2397 Fragments arranged by size: 2397 2093 1305 496 435 435 423 309 254 245 225 191 177 153 145 83 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 4172 4538 4721 5564 6232 1163 Size: 573 402 2034 366 183 843 668 4346 Fragments arranged by size: 4346 2034 843 668 573 402 366 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2299 2780 6865 7626 8654 1540 Size: 275 186 298 481 4085 761 1028 2301 Fragments arranged by size: 4085 2301 1028 761 481 298 275 186 BsiHKAI G_wGCw'C Cuts at: 2621 2903 3541 4519 5025 6421 7476 7561 8632 Size: 282 638 978 506 1396 1055 85 1071 Cuts at: 8632 2621 Size: 3404 Fragments arranged by size: 3404 1396 1071 1055 978 638 506 282 85 BsiWI C'GTAC_G Cuts at: 2198 5468 2198 Size: 3270 6145 BsmI GAATG_Cn' Cuts at: 6926 7019 6926 Size: 93 9322 BsmBI CGTCTCn'nnnn_ Cuts at: 2777 3497 6144 2777 Size: 720 2647 6048 Fragments arranged by size: 6048 2647 720 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2096 2402 2621 2694 2883 2903 Size: 399 264 42 306 219 73 189 20 Cuts at: 2903 3275 3541 3700 3903 3998 4017 4166 4288 Size: 372 266 159 203 95 19 149 122 Cuts at: 4288 4511 4519 5025 5532 5650 5727 5744 5841 Size: 223 8 506 507 118 77 17 97 Cuts at: 5841 6421 6691 6734 7476 7561 8632 1391 Size: 580 270 43 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 580 507 506 399 372 306 270 266 264 223 219 203 189 159 149 122 118 97 95 85 77 73 43 42 20 19 17 8 BspEI T'CCGG_A Cuts at: 5025 9078 5025 Size: 4053 5362 BspHI T'CATG_A Cuts at: 2983 3075 3234 7304 9034 2983 Size: 92 159 4070 1730 3364 Fragments arranged by size: 4070 3364 1730 159 92 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4179 4560 4916 5536 5615 5875 6016 6204 Size: 3335 381 356 620 79 260 141 188 Cuts at: 6204 6329 6770 844 Size: 125 441 3489 Fragments arranged by size: 3489 3335 620 441 381 356 260 188 141 125 79 BsrBI CCG'CTC Cuts at: 1394 1823 2106 2319 3243 7302 1394 Size: 429 283 213 924 4059 3507 Fragments arranged by size: 4059 3507 924 429 283 213 BsrDI GCAATG_nn' Cuts at: 65 1248 5211 6298 7910 8084 65 Size: 1183 3963 1087 1612 174 1396 Fragments arranged by size: 3963 1612 1396 1183 1087 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2908 3039 4676 5333 8059 8246 1700 Size: 110 1098 131 1637 657 2726 187 2869 Fragments arranged by size: 2869 2726 1637 1098 657 187 131 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9415 BssHII G'CGCG_C Cuts at: 1925 5610 1925 Size: 3685 5730 BssSI C'ACGA_G Cuts at: 2401 2583 3626 4813 5281 7475 8487 2401 Size: 182 1043 1187 468 2194 1012 3329 Fragments arranged by size: 3329 2194 1187 1043 1012 468 182 BstAPI GCAn_nnn'nTGC Cuts at: 794 4826 6344 794 Size: 4032 1518 3865 Fragments arranged by size: 4032 3865 1518 BstBI TT'CG_AA Cuts at: 2007 8219 2007 Size: 6212 3203 BstEII G'GTnAC_C Cuts at: 1184 3166 3320 3457 1184 Size: 1982 154 137 7142 Fragments arranged by size: 7142 1982 154 137 BstXI CCAn_nnnn'nTGG Cuts at: 2362 3190 4072 5044 5287 5482 2362 Size: 828 882 972 243 195 6295 Fragments arranged by size: 6295 972 882 828 243 195 BstYI r'GATC_y Cuts at: 1972 2927 3114 5239 5706 6745 7112 7497 7514 Size: 955 187 2125 467 1039 367 385 17 Cuts at: 7514 8966 9052 9064 9410 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2125 1977 1452 1039 955 467 385 367 346 187 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3644 4113 4150 3644 Size: 469 37 8909 Fragments arranged by size: 8909 469 37 BtgI C'CryG_G Cuts at: 513 1065 2022 2236 2382 4683 5257 5434 5680 Size: 552 957 214 146 2301 574 177 246 Cuts at: 5680 513 Size: 4248 Fragments arranged by size: 4248 2301 957 574 552 246 214 177 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2283 487 Size: 683 56 1057 7619 Fragments arranged by size: 7619 1057 683 56 BtsI GCAGTG_nn' Cuts at: 2383 3456 3636 4710 4733 4827 5344 5882 6194 Size: 1073 180 1074 23 94 517 538 312 Cuts at: 6194 6931 7725 7745 2383 Size: 737 794 20 4053 Fragments arranged by size: 4053 1074 1073 794 737 538 517 312 180 94 23 20 ClaI AT'CG_AT Cuts at: 1554 3381 1554 Size: 1827 7588 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4760 4795 5194 5229 529 Size: 35 4196 35 399 35 4715 Fragments arranged by size: 4715 4196 399 35 35 35 DraI TTT'AAA Cuts at: 6719 6719 Size: 9415 DraIII CAC_nnn'GTG Cuts at: 3679 3679 Size: 9415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2303 4268 8422 809 Size: 507 987 1965 4154 1802 Fragments arranged by size: 4154 1965 1802 987 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2777 4048 4570 Size: 54 1425 50 275 18 947 1271 522 Cuts at: 4570 4642 5098 5472 5484 6862 7751 9380 8 Size: 72 456 374 12 1378 889 1629 43 Fragments arranged by size: 1629 1425 1378 1271 947 889 522 456 374 275 72 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2777 6862 1537 Size: 275 965 4085 4090 Fragments arranged by size: 4090 4085 965 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3593 4899 7345 1353 Size: 2240 1306 2446 3423 Fragments arranged by size: 3423 2446 2240 1306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3485 3533 4223 6090 6497 8002 Size: 1064 138 1896 48 690 1867 407 1505 Cuts at: 8002 8376 8522 387 Size: 374 146 1280 Fragments arranged by size: 1896 1867 1505 1280 1064 690 407 374 146 138 48 EcoICRI GAG'CTC Cuts at: 2901 5023 2901 Size: 2122 7293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2335 2411 2638 2727 3203 3785 4077 Size: 393 703 76 227 89 476 582 292 Cuts at: 4077 4380 4401 4840 5066 5309 5447 5766 6263 Size: 303 21 439 226 243 138 319 497 Cuts at: 6263 6616 7478 8075 8862 9130 1239 Size: 353 862 597 787 268 1524 Fragments arranged by size: 1524 862 787 703 597 582 497 476 439 393 353 319 303 292 268 243 227 226 138 89 76 21 EcoNI CCTnn'n_nnAGG Cuts at: 2646 5907 6387 2646 Size: 3261 480 5674 Fragments arranged by size: 5674 3261 480 EcoO109I rG'GnC_Cy Cuts at: 2063 2092 2093 2467 2733 2997 3648 3837 3994 Size: 29 1 374 266 264 651 189 157 Cuts at: 3994 3995 4285 4973 5195 6514 6590 2063 Size: 1 290 688 222 1319 76 4888 Fragments arranged by size: 4888 1319 688 651 374 290 266 264 222 189 157 76 29 1 1 EcoRI G'AATT_C Cuts at: 3056 3293 4391 6724 7102 3056 Size: 237 1098 2333 378 5369 Fragments arranged by size: 5369 2333 1098 378 237 FspI TGC'GCA Cuts at: 3728 4475 7921 3728 Size: 747 3446 5222 Fragments arranged by size: 5222 3446 747 FspAI rTGC'GCAy Cuts at: 3728 3728 Size: 9415 HaeII r_GCGC'y Cuts at: 1678 2173 2348 3040 3524 3583 4587 8562 1678 Size: 495 175 692 484 59 1004 3975 2531 Fragments arranged by size: 3975 2531 1004 692 495 484 175 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2426 2458 3128 3160 4325 Size: 32 135 32 854 32 670 32 1165 Cuts at: 4325 4357 6349 6381 6506 6538 8056 8088 8130 Size: 32 1992 32 125 32 1518 32 42 Cuts at: 8130 8162 1373 Size: 32 2626 Fragments arranged by size: 2626 1992 1518 1165 854 670 135 125 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 6759 7005 669 Size: 988 281 370 4451 246 3079 Fragments arranged by size: 4451 3079 988 370 281 246 HindIII A'AGCT_T Cuts at: 748 3128 748 Size: 2380 7035 HpaI GTT'AAC Cuts at: 7005 7005 Size: 9415 KpnI G_GTAC'C Cuts at: 6740 6740 Size: 9415 MfeI C'AATT_G Cuts at: 5268 7014 5268 Size: 1746 7669 MluI A'CGCG_T Cuts at: 2275 9291 2275 Size: 7016 2399 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2148 2430 2988 3372 4302 5217 8529 8713 Size: 843 282 558 384 930 915 3312 184 Cuts at: 8713 1305 Size: 2007 Fragments arranged by size: 3312 2007 930 915 843 558 384 282 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4050 4644 5486 10 Size: 54 1425 343 2218 594 842 3939 Fragments arranged by size: 3939 2218 1425 842 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3233 3236 4070 4430 4526 5042 5174 Size: 648 2067 3 834 360 96 516 132 Cuts at: 5174 5480 5796 5802 7373 7732 7891 518 Size: 306 316 6 1571 359 159 2042 Fragments arranged by size: 2067 2042 1571 834 648 516 360 359 316 306 159 132 96 6 3 MspA1I CmG'CkG Cuts at: 1606 2100 2142 4186 4338 4832 5055 5259 5536 Size: 494 42 2044 152 494 223 204 277 Cuts at: 5536 6302 6529 6602 6831 7508 8656 8901 1606 Size: 766 227 73 229 677 1148 245 2120 Fragments arranged by size: 2120 2044 1148 766 677 494 494 277 245 229 227 223 204 152 73 42 NaeI GCC'GGC Cuts at: 3041 3041 Size: 9415 NcoI C'CATG_G Cuts at: 513 1065 2022 4683 5434 513 Size: 552 957 2661 751 4494 Fragments arranged by size: 4494 2661 957 751 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9415 NgoMIV G'CCGG_C Cuts at: 3039 3039 Size: 9415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9415 NotI GC'GGCC_GC Cuts at: 6862 6862 Size: 9415 NspI r_CATG'y Cuts at: 1132 1229 6773 8318 1132 Size: 97 5544 1545 2229 Fragments arranged by size: 5544 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4997 1348 Size: 3649 5766 PciI A'CATG_T Cuts at: 8314 8314 Size: 9415 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 4751 5524 5539 6267 1108 Size: 1134 2509 773 15 728 4256 Fragments arranged by size: 4256 2509 1134 773 728 15 PfoI T'CCnGG_A Cuts at: 2312 3885 5424 6510 2312 Size: 1573 1539 1086 5217 Fragments arranged by size: 5217 1573 1539 1086 PmeI GTTT'AAAC Cuts at: 6719 6719 Size: 9415 PmlI CAC'GTG Cuts at: 5739 5739 Size: 9415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2885 2917 7484 7516 8997 9029 2885 Size: 32 4567 32 1481 32 3271 Fragments arranged by size: 4567 3271 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 2733 2997 3837 4973 6514 6590 2467 Size: 266 264 840 1136 1541 76 5292 Fragments arranged by size: 5292 1541 1136 840 266 264 76 PshAI GACnn'nnGTC Cuts at: 1613 2119 3464 1613 Size: 506 1345 7564 Fragments arranged by size: 7564 1345 506 PsiI TTA'TAA Cuts at: 6985 6985 Size: 9415 PspOMI G'GGCC_C Cuts at: 2092 3994 4284 5723 2092 Size: 1902 290 1439 5784 Fragments arranged by size: 5784 1902 1439 290 PspXI vC'TCGA_Gb Cuts at: 2687 5417 2687 Size: 2730 6685 PstI C_TGCA'G Cuts at: 830 2743 5203 6767 830 Size: 1913 2460 1564 3478 Fragments arranged by size: 3478 2460 1913 1564 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9415 PvuII CAG'CTG Cuts at: 4186 5055 5536 4186 Size: 869 481 8065 Fragments arranged by size: 8065 869 481 SacI G_AGCT'C Cuts at: 2903 5025 2903 Size: 2122 7293 SacII CC_GC'GG Cuts at: 5260 5260 Size: 9415 SalI G'TCGA_C Cuts at: 1936 6757 1936 Size: 4821 4594 SanDI GG'GwC_CC Cuts at: 2997 4973 6514 6590 2997 Size: 1976 1541 76 5822 Fragments arranged by size: 5822 1976 1541 76 SapI GCTCTTCn'nnn_ Cuts at: 3593 4899 3593 Size: 1306 8109 SbfI CC_TGCA'GG Cuts at: 6767 6767 Size: 9415 ScaI AGT'ACT Cuts at: 3287 4777 7663 9375 3287 Size: 1490 2886 1712 3327 Fragments arranged by size: 3327 2886 1712 1490 SexAI A'CCwGG_T Cuts at: 4125 4125 Size: 9415 SfcI C'TryA_G Cuts at: 826 1045 2739 5199 6763 7898 8579 8770 826 Size: 219 1694 2460 1564 1135 681 191 1471 Fragments arranged by size: 2460 1694 1564 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 5382 5382 Size: 9415 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9415 SgrAI Cr'CCGG_yG Cuts at: 1700 3039 1700 Size: 1339 8076 SmaI CCC'GGG Cuts at: 1872 4020 4978 6185 6588 6742 1872 Size: 2148 958 1207 403 154 4545 Fragments arranged by size: 4545 2148 1207 958 403 154 SmlI C'TyrA_G Cuts at: 820 1017 1943 2687 2801 3917 5417 5702 6647 Size: 197 926 744 114 1116 1500 285 945 Cuts at: 6647 7519 8420 8682 820 Size: 872 901 262 1553 Fragments arranged by size: 1553 1500 1116 945 926 901 872 744 285 262 197 114 SnaBI TAC'GTA Cuts at: 493 6712 7206 493 Size: 6219 494 2702 Fragments arranged by size: 6219 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9415 SphI G_CATG'C Cuts at: 1132 6773 1132 Size: 5641 3774 SspI AAT'ATT Cuts at: 5 52 7339 5 Size: 47 7287 2081 Fragments arranged by size: 7287 2081 47 StuI AGG'CCT Cuts at: 1517 2574 2922 1517 Size: 1057 348 8010 Fragments arranged by size: 8010 1057 348 StyI C'CwwG_G Cuts at: 513 1065 2022 2694 2718 4542 4579 4683 4863 Size: 552 957 672 24 1824 37 104 180 Cuts at: 4863 5434 5941 6607 6854 513 Size: 571 507 666 247 3074 Fragments arranged by size: 3074 1824 957 672 666 571 552 507 247 180 104 37 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2527 2636 3188 3638 3797 4476 5126 Size: 1255 947 109 552 450 159 679 650 Cuts at: 5126 5321 6336 7442 7459 7612 7797 8242 325 Size: 195 1015 1106 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1106 1015 947 679 650 552 450 445 195 185 159 153 109 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3285 4775 7661 Size: 274 80 33 51 1443 1308 1490 2886 Cuts at: 7661 9373 96 Size: 1712 138 Fragments arranged by size: 2886 1712 1490 1443 1308 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 4423 4455 5296 5328 9375 9407 2430 Size: 32 1961 32 841 32 4047 32 2438 Fragments arranged by size: 4047 2438 1961 841 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9415 XbaI T'CTAG_A Cuts at: 4798 6751 4798 Size: 1953 7462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 3599 4681 2131 Size: 1468 1082 6865 Fragments arranged by size: 6865 1468 1082 XhoI C'TCGA_G Cuts at: 1943 2687 5417 1943 Size: 744 2730 5941 Fragments arranged by size: 5941 2730 744 XmaI C'CCGG_G Cuts at: 1870 4018 4976 6183 6586 6740 1870 Size: 2148 958 1207 403 154 4545 Fragments arranged by size: 4545 2148 1207 958 403 154 XmnI GAAnn'nnTTC Cuts at: 4727 7544 4727 Size: 2817 6598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7223 276 Size: 53 83 186 936 5689 2468 Fragments arranged by size: 5689 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BstZ17I EcoRV FalI FseI KasI NarI NruI NsiI PacI PsrI RsrII SfoI SrfI SwaI