Restriction map for
pFN21ASDA1785
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII AccII AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI AspEI AspI AviII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FbaI FseI FspI Hin1I HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1496 GGTACC G^GTACC
AccI 766 GTAGAC GT^[AC][GT]AC
AflII 1417 CTTAAG C^TTAAG
AflIII 1483 ACATGT A^C[GA][CT]GT
Alw44I 1652 GTGCAC G^TGCAC
ApaLI 1652 GTGCAC G^TGCAC
Asp718I 1496 GGTACC G^GTACC
AspHI 1652 GTGCAC G[AT]GC[AT]^C
AvaI 32 CTCGGG C^[CT]CG[GA]G
BalI 378 TGGCCA TGG^CCA
BamHI 1116 GGATCC G^GATCC
BfrI 1417 CTTAAG C^TTAAG
BglII 780 AGATCT A^GATCT
BsaAI 450 TACGTG [CT]AC^GT[GA]
BsiHKAI 1652 GTGCAC G[AT]GC[AT]^C
BsmFI 1589 GTCCC GGGAC(10/14)
BsmI 956 GAATGC GAATGC(1/-1)
BsoBI 32 CTCGGG C^[CT]CG[GA]G
BspLU11I 1483 ACATGT A^CATGT
BssKI 146 CCAGG ^CC[ACGT]GG
BssSI 1772 CTCGTG CACGAG(-5/-1)
BstAPI 1238 GCATACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 146 CCAGG CC^[AT]GG
BstXI 59 CCAAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1034 GCGC GCG^C
DraII 1319 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1319 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 172 GAATTC G^AATTC
EcoRII 146 CCAGG ^CC[AT]GG
EcoT22I 1068 ATGCAT ATGCA^T
HaeII 1033 GGCGCT [GA]GCGC^[CT]
HapII 48 CCGG C^CGG
HgaI 1253 GCGTC GACGC(5/10)
HhaI 1034 GCGC GCG^C
HinP1I 1034 GCGC G^CGC
HincII 325 GTCAAC GT[CT]^[GA]AC
HindII 325 GTCAAC GT[CT]^[GA]AC
HpaII 48 CCGG C^CGG
HphI 56 TCACC GGTGA(8/7)
KpnI 1496 GGTACC GGTAC^C
MluNI 378 TGGCCA TGG^CCA
MscI 378 TGGCCA TGG^CCA
MspA1I 1295 CAGCTG C[AC]G^C[GT]G
MspI 48 CCGG C^CGG
MvaI 146 CCAGG CC^[AT]GG
NsiI 1068 ATGCAT ATGCA^T
NspI 1483 ACATGT [GA]CATG^[CT]
PshAI 1582 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 272 CTGCAG CTGCA^G
PvuII 1295 CAGCTG CAG^CTG
SapI 916 GAAGAGC GCTCTTC(1/4)
ScrFI 146 CCAGG CC^[ACGT]GG
SmlI 1417 CTTAAG C^T[CT][GA]AG
StuI 1197 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 569 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
1046 CAGACTCTG
AseI 733 ATTAAT AT^TAAT
969 ATTAAT
AsnI 733 ATTAAT AT^TAAT
969 ATTAAT
Asp700I 979 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1440 GAATAACTTC
AvaII 294 GGTCC G^G[AT]CC
991 GGTCC
BanI 155 GGCACC G^G[CT][GA]CC
1496 GGTACC
BanII 213 GGGCTC G[GA]GC[CT]^C
248 GGGCTC
BsaJI 896 CCAAGG C^C[ACGT][ACGT]GG
1429 CCAAGG
BseRI 353 CTCCTC GAGGAG(10/8)
85 GAGGAG
BsgI 566 GTGCAG GTGCAG(16/14)
911 GTGCAG
BsmAI 1571 GAGAC GTCTC(1/5)
1828 GAGAC
BspHI 590 TCATGA T^CATGA
1628 TCATGA
EaeI 140 TGGCCG [CT]^GGCC[GA]
378 TGGCCA
EcoT14I 896 CCAAGG C^C[AT][AT]GG
1429 CCAAGG
PshBI 733 ATTAAT AT^TAAT
969 ATTAAT
RcaI 590 TCATGA T^CATGA
1628 TCATGA
SfcI 272 CTGCAG C^T[GA][CT]AG
1180 CTACAG
SspI 682 AATATT AAT^ATT
844 AATATT
StyI 896 CCAAGG C^C[AT][AT]GG
1429 CCAAGG
TaqI 360 TCGA T^CGA
374 TCGA
TthHB8I 360 TCGA T^CGA
374 TCGA
XbaI 741 TCTAGA T^CTAGA
1374 TCTAGA
XmnI 979 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1440 GAATAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BmyI BsiYI
BslI Bsp1286I BsrDI BsrI BstF5I
BstYI Cac8I Cfr13I DdeI DpnII
EarI Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII