(Circular) (Six-base) MAPSORT of: pFN21ASDA1783.seq Check: 4065 from: 1 to: 14017 pFN21ASDA1783 14017 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2941 6260 10431 10858 11148 2941 Size: 3319 4171 427 290 5810 Fragments arranged by size: 5810 4171 3319 427 290 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 11827 278 Size: 53 83 186 936 10291 2468 Fragments arranged by size: 10291 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6228 10238 11360 1937 Size: 4291 4010 1122 4594 Fragments arranged by size: 4594 4291 4010 1122 Acc65I G'GTAC_C Cuts at: 3212 5083 6321 11338 3212 Size: 1871 1238 5017 5891 Fragments arranged by size: 5891 5017 1871 1238 AclI AA'CG_TT Cuts at: 1563 12144 12517 1563 Size: 10581 373 3063 Fragments arranged by size: 10581 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3729 4928 6533 7147 8091 8956 9323 9401 Size: 2097 1199 1605 614 944 865 367 78 Cuts at: 9401 10121 10712 11138 12080 13464 13732 1632 Size: 720 591 426 942 1384 268 1917 Fragments arranged by size: 2097 1917 1605 1384 1199 944 942 865 720 614 591 426 367 268 78 AfeI AGC'GCT Cuts at: 2625 6212 7026 2625 Size: 3587 814 9616 Fragments arranged by size: 9616 3587 814 AflII C'TTAA_G Cuts at: 820 1017 9458 820 Size: 197 8441 5379 Fragments arranged by size: 8441 5379 197 AflIII A'CryG_T Cuts at: 1203 2469 5938 12916 13893 1203 Size: 1266 3469 6978 977 1327 Fragments arranged by size: 6978 3469 1327 1266 977 AgeI A'CCGG_T Cuts at: 4311 12848 4311 Size: 8537 5480 AhdI GACnn_n'nnGTC Cuts at: 12746 12746 Size: 14017 AleI CACnn'nnGTG Cuts at: 1166 4470 5092 5297 5589 1166 Size: 3304 622 205 292 9594 Fragments arranged by size: 9594 3304 622 292 205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7045 9239 9657 1184 Size: 5861 2194 418 5544 Fragments arranged by size: 5861 5544 2194 418 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10865 10897 10865 Size: 32 13985 AlwNI CAG_nnn'CTG Cuts at: 2056 2267 2496 2658 5398 5660 5839 6049 6115 Size: 211 229 162 2740 262 179 210 66 Cuts at: 6115 6187 6223 6382 6403 6688 7751 8749 10714 Size: 72 36 159 21 285 1063 998 1965 Cuts at: 10714 10834 10909 11029 11098 11245 11760 13332 2056 Size: 120 75 120 69 147 515 1572 2741 Fragments arranged by size: 2741 2740 1965 1572 1063 998 515 285 262 229 211 210 179 162 159 147 120 120 75 72 69 66 36 21 ApaI G_GGCC'C Cuts at: 2328 2424 2709 3095 6928 7093 7641 8501 8645 Size: 96 285 386 3833 165 548 860 144 Cuts at: 8645 8694 8724 11169 2328 Size: 49 30 2445 5176 Fragments arranged by size: 5176 3833 2445 860 548 386 285 165 144 96 49 30 ApaLI G'TGCA_C Cuts at: 12074 13230 12074 Size: 1156 12861 ApoI r'AATT_y Cuts at: 1493 9857 10535 11326 11552 11704 13813 1493 Size: 8364 678 791 226 152 2109 1697 Fragments arranged by size: 8364 2109 1697 791 678 226 152 AscI GG'CGCG_CC Cuts at: 2118 2118 Size: 14017 AseI AT'TA_AT Cuts at: 160 1032 12571 160 Size: 872 11539 1606 Fragments arranged by size: 11539 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 14017 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2553 2676 2746 2781 2829 2913 Size: 73 123 487 123 70 35 48 84 Cuts at: 2913 3494 4030 7075 7086 7260 7932 8494 8801 Size: 581 536 3045 11 174 672 562 307 Cuts at: 8801 9159 9202 9411 10062 10191 11342 1870 Size: 358 43 209 651 129 1151 4545 Fragments arranged by size: 4545 3045 1151 672 651 581 562 536 487 358 307 209 174 129 123 123 84 73 70 48 43 35 11 AvrII C'CTAG_G Cuts at: 3962 3962 Size: 14017 BamHI G'GATC_C Cuts at: 3669 6776 7227 11347 11714 3669 Size: 3107 451 4120 367 5972 Fragments arranged by size: 5972 4120 3107 451 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2346 2879 2953 3071 3212 Size: 325 217 627 559 533 74 118 141 Cuts at: 3212 3504 3971 4132 4148 5083 5257 5285 5446 Size: 292 467 161 16 935 174 28 161 Cuts at: 5446 5984 6095 6321 7252 7628 7771 8597 9914 Size: 538 111 226 931 376 143 826 1317 Cuts at: 9914 10347 11338 12793 618 Size: 433 991 1455 1842 Fragments arranged by size: 1842 1455 1317 991 935 931 826 627 559 538 533 467 433 376 325 292 226 217 174 161 161 143 141 118 111 74 28 16 BanII G_rGCy'C Cuts at: 1391 2328 2424 2594 2709 2780 2827 2920 3095 Size: 937 96 170 115 71 47 93 175 Cuts at: 3095 3142 3495 3921 3944 4926 5784 6198 6861 Size: 47 353 426 23 982 858 414 663 Cuts at: 6861 6928 7093 7603 7641 8501 8645 8694 8724 Size: 67 165 510 38 860 144 49 30 Cuts at: 8724 11055 11169 11336 1391 Size: 2331 114 167 4072 Fragments arranged by size: 4072 2331 982 937 860 858 663 510 426 414 353 175 170 167 165 144 115 114 96 93 71 67 49 47 47 38 30 23 BbeI G_GCGC'C Cuts at: 2350 2883 3075 3975 4136 7256 7632 10351 2350 Size: 533 192 900 161 3120 376 2719 6016 Fragments arranged by size: 6016 3120 2719 900 533 376 192 161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4188 4499 4645 6075 6521 8079 9132 Size: 971 2289 311 146 1430 446 1558 1053 Cuts at: 9132 11254 928 Size: 2122 3691 Fragments arranged by size: 3691 2289 2122 1558 1430 1053 971 446 311 146 BbvCI CC'TCA_GC Cuts at: 4370 5457 6726 7577 7966 8340 9521 9980 10124 Size: 1087 1269 851 389 374 1181 459 144 Cuts at: 10124 10208 10796 4370 Size: 84 588 7591 Fragments arranged by size: 7591 1269 1181 1087 851 588 459 389 374 144 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3034 3068 4147 4181 5341 5375 7913 Size: 34 1097 34 1079 34 1160 34 2538 Cuts at: 7913 7947 10390 10424 12208 12242 1903 Size: 34 2443 34 1784 34 3678 Fragments arranged by size: 3678 2538 2443 1784 1160 1097 1079 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5528 9050 11909 13119 5528 Size: 3522 2859 1210 6426 Fragments arranged by size: 6426 3522 2859 1210 BclI T'GATC_A Cuts at: 1548 3619 7335 1548 Size: 2071 3716 8230 Fragments arranged by size: 8230 3716 2071 BfrBI ATG'CAT Cuts at: 4446 4446 Size: 14017 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2071 2544 2909 Size: 107 122 71 631 605 399 473 365 Cuts at: 2909 3023 3105 3528 4400 4644 4958 6032 6918 Size: 114 82 423 872 244 314 1074 886 Cuts at: 6918 6932 7079 7088 7533 7943 8440 8496 8527 Size: 14 147 9 445 410 497 56 31 Cuts at: 8527 9357 9428 9580 10778 12628 136 Size: 830 71 152 1198 1850 1525 Fragments arranged by size: 1850 1525 1198 1074 886 872 830 631 605 497 473 445 423 410 399 365 314 244 152 147 122 114 107 82 71 71 56 31 14 9 BglII A'GATC_T Cuts at: 7548 14012 7548 Size: 6464 7553 BlpI GC'TnA_GC Cuts at: 3532 4109 4746 6495 6944 7402 8346 11144 11194 Size: 577 637 1749 449 458 944 2798 50 Cuts at: 11194 11434 3532 Size: 240 6115 Fragments arranged by size: 6115 2798 1749 944 637 577 458 449 240 50 Bme1580I G_kGCm'C Cuts at: 1790 2328 2424 2709 3095 3777 4479 4937 5987 Size: 538 96 285 386 682 702 458 1050 Cuts at: 5987 6018 6736 6928 7093 7641 7745 8501 8514 Size: 31 718 192 165 548 104 756 13 Cuts at: 8514 8602 8645 8694 8724 11169 12078 13234 1790 Size: 88 43 49 30 2445 909 1156 2573 Fragments arranged by size: 2573 2445 1156 1050 909 756 718 702 682 548 538 458 386 285 192 165 104 96 88 49 43 31 30 13 BmgBI CAC'GTC Cuts at: 1318 2472 5932 9223 10627 1318 Size: 1154 3460 3291 1404 4708 Fragments arranged by size: 4708 3460 3291 1404 1154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2847 3593 4440 Size: 416 53 83 399 24 1430 746 847 Cuts at: 4440 5779 9178 10663 11748 12706 442 Size: 1339 3399 1485 1085 958 1753 Fragments arranged by size: 3399 1753 1485 1430 1339 1085 958 847 746 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 10841 1056 Size: 9785 4232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2833 3895 4828 7195 2833 Size: 1062 933 2367 9655 Fragments arranged by size: 9655 2367 1062 933 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2372 2648 2782 3621 3865 4112 4124 Size: 839 294 276 134 839 244 247 12 Cuts at: 4124 4148 4556 4705 5120 5360 5371 5552 5732 Size: 24 408 149 415 240 11 181 180 Cuts at: 5732 5861 5993 6209 6371 6616 6803 7130 7268 Size: 129 132 216 162 245 187 327 138 Cuts at: 7268 7270 7449 7919 9389 9590 10219 10307 11174 Size: 2 179 470 1470 201 629 88 867 Cuts at: 11174 12677 1239 Size: 1503 2579 Fragments arranged by size: 2579 1503 1470 867 839 839 629 470 415 408 327 294 276 247 245 244 240 216 201 187 181 180 179 162 149 138 134 132 129 88 24 12 11 2 Bpu10I CC'TnA_GC Cuts at: 3943 4370 4486 4526 5457 6104 6726 7577 7966 Size: 427 116 40 931 647 622 851 389 Cuts at: 7966 8340 9521 9980 10124 10208 10796 3943 Size: 374 1181 459 144 84 588 7164 Fragments arranged by size: 7164 1181 931 851 647 622 588 459 427 389 374 144 116 84 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3095 4226 4733 6971 9248 12142 13007 13305 3095 Size: 1131 507 2238 2277 2894 865 298 3807 Fragments arranged by size: 3807 2894 2277 2238 1131 865 507 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3896 3998 4020 4709 5152 8048 8872 Size: 617 2397 102 22 689 443 2896 824 Cuts at: 8872 10652 12680 882 Size: 1780 2028 2219 Fragments arranged by size: 2896 2397 2219 2028 1780 824 689 617 443 102 22 BsaAI yAC'GTr Cuts at: 493 1204 10846 11314 11808 493 Size: 711 9642 468 494 2702 Fragments arranged by size: 9642 2702 711 494 468 BsaBI GATnn'nnATC Cuts at: 14011 14011 Size: 14017 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2257 2347 2447 2880 Size: 53 83 186 936 724 90 100 433 Cuts at: 2880 3072 3689 3972 4133 5132 5190 5526 6537 Size: 192 617 283 161 999 58 336 1011 Cuts at: 6537 7253 7629 10348 11824 12206 12841 275 Size: 716 376 2719 1476 382 635 1451 Fragments arranged by size: 2719 1476 1451 1011 999 936 724 716 635 617 433 382 376 336 283 192 186 161 100 90 83 58 53 BsaWI w'CCGG_w Cuts at: 2369 2500 2762 3253 4066 4311 4825 9042 9608 Size: 131 262 491 813 245 514 4217 566 Cuts at: 9608 9876 11033 12450 12848 13122 13269 13680 2369 Size: 268 1157 1417 398 274 147 411 2706 Fragments arranged by size: 4217 2706 1417 1157 813 566 514 491 411 398 274 268 262 245 147 131 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2084 2108 2114 4680 Size: 30 165 30 675 6 24 6 2566 Cuts at: 4680 4710 5333 5363 5405 5423 5435 5453 9011 Size: 30 623 30 42 18 12 18 3558 Cuts at: 9011 9041 9943 9973 10478 10508 10865 10895 11028 Size: 30 902 30 505 30 357 30 133 Cuts at: 11028 11058 11072 11102 1178 Size: 30 14 30 4093 Fragments arranged by size: 4093 3558 2566 902 675 623 505 357 165 133 42 30 30 30 30 30 30 30 30 30 30 24 18 18 14 12 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 2388 2817 3041 4450 4955 4993 4996 Size: 1183 19 429 224 1409 505 38 3 Cuts at: 4996 5251 5426 5429 5432 6206 7184 7383 8078 Size: 255 175 3 3 774 978 199 695 Cuts at: 8078 8219 8222 8225 8228 8231 8234 8237 8240 Size: 141 3 3 3 3 3 3 3 Cuts at: 8240 8243 8246 8255 8268 8271 8652 8987 9509 Size: 3 3 9 13 3 381 335 522 Cuts at: 9509 9691 9968 10502 11171 1186 Size: 182 277 534 669 4032 Fragments arranged by size: 4032 1409 1183 978 774 695 669 534 522 505 429 381 335 277 255 224 199 182 175 141 38 19 13 9 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2049 2123 2267 2383 2755 3013 3541 3930 4984 Size: 74 144 116 372 258 528 389 1054 Cuts at: 4984 5046 5600 5860 6115 6375 6472 6545 6590 Size: 62 554 260 255 260 97 73 45 Cuts at: 6590 6604 6705 6879 6921 7045 7104 7220 7363 Size: 14 101 174 42 124 59 116 143 Cuts at: 7363 7487 7596 7793 7863 8450 8584 8627 8874 Size: 124 109 197 70 587 134 43 247 Cuts at: 8874 9082 9337 9895 10286 11084 11300 13220 13716 Size: 208 255 558 391 798 216 1920 496 Cuts at: 13716 2049 Size: 2350 Fragments arranged by size: 2350 1920 1054 798 587 558 554 528 496 391 389 372 260 260 258 255 255 247 216 208 197 174 144 143 134 124 124 116 116 109 101 97 74 73 70 62 59 45 43 42 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3780 3808 3831 5939 6218 7265 7698 Size: 573 2044 28 23 2108 279 1047 433 Cuts at: 7698 8537 9245 9386 9653 9965 10171 10502 10550 Size: 839 708 141 267 312 206 331 48 Cuts at: 10550 10619 10652 1163 Size: 69 33 4528 Fragments arranged by size: 4528 2108 2044 1047 839 708 573 433 331 312 279 267 206 141 69 48 33 28 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2146 2178 2338 2392 3191 3657 Size: 275 186 145 32 160 54 799 466 Cuts at: 3657 4121 7185 10178 11467 12228 13256 1540 Size: 464 3064 2993 1289 761 1028 2301 Fragments arranged by size: 3064 2993 2301 1289 1028 799 761 466 464 275 186 160 145 54 32 BsiHKAI G_wGCw'C Cuts at: 4460 6198 6861 10320 12078 12163 13234 4460 Size: 1738 663 3459 1758 85 1071 5243 Fragments arranged by size: 5243 3459 1758 1738 1071 663 85 BsmI GAATG_Cn' Cuts at: 2725 4954 7431 11528 11621 2725 Size: 2229 2477 4097 93 5121 Fragments arranged by size: 5121 4097 2477 2229 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4164 5123 10906 4164 Size: 959 5783 7275 Fragments arranged by size: 7275 5783 959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2424 2594 2709 2780 2827 2920 Size: 399 538 96 170 115 71 47 93 Cuts at: 2920 3095 3142 3495 3777 3921 3944 4460 4479 Size: 175 47 353 282 144 23 516 19 Cuts at: 4479 4926 4937 5784 5987 6018 6198 6736 6861 Size: 447 11 847 203 31 180 538 125 Cuts at: 6861 6928 7093 7603 7641 7745 8501 8514 8602 Size: 67 165 510 38 104 756 13 88 Cuts at: 8602 8645 8694 8724 10320 11055 11169 11336 12078 Size: 43 49 30 1596 735 114 167 742 Cuts at: 12078 12163 13234 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1596 1071 847 756 742 735 538 538 516 510 447 399 353 282 203 180 175 170 167 165 144 125 115 114 104 96 93 88 85 71 67 49 47 47 43 38 31 30 23 19 13 11 BspEI T'CCGG_A Cuts at: 2369 2500 9042 9608 9876 11033 13680 2369 Size: 131 6542 566 268 1157 2647 2706 Fragments arranged by size: 6542 2706 2647 1157 566 268 131 BspHI T'CATG_A Cuts at: 11906 13636 11906 Size: 1730 12287 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2941 3574 6240 6260 9087 10147 10431 10842 Size: 2097 633 2666 20 2827 1060 284 411 Cuts at: 10842 10858 11148 11372 844 Size: 16 290 224 3489 Fragments arranged by size: 3489 2827 2666 2097 1060 633 411 290 284 224 20 16 BsrBI CCG'CTC Cuts at: 1394 1823 2532 2999 3118 3576 3876 4750 4801 Size: 429 709 467 119 458 300 874 51 Cuts at: 4801 4807 9061 9158 9647 11904 1394 Size: 6 4254 97 489 2257 3507 Fragments arranged by size: 4254 3507 2257 874 709 489 467 458 429 300 119 97 51 6 BsrDI GCAATG_nn' Cuts at: 65 1248 7743 11109 12512 12686 65 Size: 1183 6495 3366 1403 174 1396 Fragments arranged by size: 6495 3366 1403 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 2170 2842 4311 5226 8915 9556 Size: 110 264 96 672 1469 915 3689 641 Cuts at: 9556 9637 10350 10497 10641 12661 12848 1700 Size: 81 713 147 144 2020 187 2869 Fragments arranged by size: 3689 2869 2020 1469 915 713 672 641 264 187 147 144 110 96 81 BsrGI T'GTAC_A Cuts at: 96 96 Size: 14017 BssHII G'CGCG_C Cuts at: 1925 2118 1925 Size: 193 13824 BssSI C'ACGA_G Cuts at: 3326 3380 3434 3756 3953 12077 13089 3326 Size: 54 54 322 197 8124 1012 4254 Fragments arranged by size: 8124 4254 1012 322 197 54 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 4995 6403 7042 7909 8901 9397 10834 794 Size: 4201 1408 639 867 992 496 1437 3977 Fragments arranged by size: 4201 3977 1437 1408 992 867 639 496 BstBI TT'CG_AA Cuts at: 2007 12821 2007 Size: 10814 3203 BstEII G'GTnAC_C Cuts at: 1184 5591 9268 10046 1184 Size: 4407 3677 778 5155 Fragments arranged by size: 5155 4407 3677 778 BstXI CCAn_nnnn'nTGG Cuts at: 4472 6367 6713 7018 9908 10024 11047 4472 Size: 1895 346 305 2890 116 1023 7442 Fragments arranged by size: 7442 2890 1895 1023 346 305 116 BstYI r'GATC_y Cuts at: 1972 3640 3669 5055 6486 6776 7227 7548 9009 Size: 1668 29 1386 1431 290 451 321 1461 Cuts at: 9009 10081 11347 11714 12099 12116 13568 13654 13666 Size: 1072 1266 367 385 17 1452 86 12 Cuts at: 13666 14012 1972 Size: 346 1977 Fragments arranged by size: 1977 1668 1461 1452 1431 1386 1266 1072 451 385 367 346 321 290 86 29 17 12 Bsu36I CC'TnA_GG Cuts at: 2080 4049 4335 6573 8048 8546 8990 10521 10822 Size: 1969 286 2238 1475 498 444 1531 301 Cuts at: 10822 2080 Size: 5275 Fragments arranged by size: 5275 2238 1969 1531 1475 498 444 301 286 BtgI C'CryG_G Cuts at: 513 1065 2022 2146 2338 3087 3221 3298 3352 Size: 552 957 124 192 749 134 77 54 Cuts at: 3352 3732 4039 5163 5550 5587 5979 7659 7764 Size: 380 307 1124 387 37 392 1680 105 Cuts at: 7764 8377 8850 8960 9247 9532 513 Size: 613 473 110 287 285 4998 Fragments arranged by size: 4998 1680 1124 957 749 613 552 473 392 387 380 307 287 285 192 134 124 110 105 77 54 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 9466 487 Size: 683 56 8240 5038 Fragments arranged by size: 8240 5038 683 56 BtsI GCAGTG_nn' Cuts at: 2229 3892 4465 4477 4913 5998 6839 6959 6980 Size: 1663 573 12 436 1085 841 120 21 Cuts at: 6980 7277 7445 8271 8789 10183 10664 11284 11533 Size: 297 168 826 518 1394 481 620 249 Cuts at: 11533 12327 12347 2229 Size: 794 20 3899 Fragments arranged by size: 3899 1663 1394 1085 841 826 794 620 573 518 481 436 297 249 168 120 21 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 14017 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 13982 DraI TTT'AAA Cuts at: 5688 11321 5688 Size: 5633 8384 DraIII CAC_nnn'GTG Cuts at: 6561 8021 9779 10370 11295 6561 Size: 1460 1758 591 925 9283 Fragments arranged by size: 9283 1758 1460 925 591 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 8864 13024 809 Size: 507 4223 3325 4160 1802 Fragments arranged by size: 4223 4160 3325 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2157 2175 Size: 54 1425 50 275 18 273 54 18 Cuts at: 2175 2215 2335 2389 2844 3188 3571 4118 4603 Size: 40 120 54 455 344 383 547 485 Cuts at: 4603 4645 4968 5140 5196 5734 6033 6501 6839 Size: 42 323 172 56 538 299 468 338 Cuts at: 6839 7119 7182 7815 8169 8367 8380 8441 8577 Size: 280 63 633 354 198 13 61 136 Cuts at: 8577 8740 9153 9561 10175 10779 11464 12353 13982 Size: 163 413 408 614 604 685 889 1629 Cuts at: 13982 8 Size: 43 Fragments arranged by size: 1629 1425 889 685 633 614 604 547 538 485 468 455 413 408 383 354 344 338 323 299 280 275 273 198 172 163 136 120 63 61 56 54 54 54 50 43 42 40 18 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2175 2335 2389 3188 4118 7182 10175 Size: 275 363 160 54 799 930 3064 2993 Cuts at: 10175 11464 1537 Size: 1289 4090 Fragments arranged by size: 4090 3064 2993 1289 930 799 363 275 160 54 EarI CTCTTCn'nnn_ Cuts at: 1353 2373 4241 5225 5387 5567 9377 10550 10556 Size: 1020 1868 984 162 180 3810 1173 6 Cuts at: 10556 10988 11114 11947 1353 Size: 432 126 833 3423 Fragments arranged by size: 3810 3423 1868 1173 1020 984 833 432 180 162 126 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3908 6536 9079 9278 10369 12604 Size: 1064 138 2319 2628 2543 199 1091 2235 Cuts at: 12604 12978 13124 387 Size: 374 146 1280 Fragments arranged by size: 2628 2543 2319 2235 1280 1091 1064 374 199 146 138 EcoICRI GAG'CTC Cuts at: 6196 6859 6196 Size: 663 13354 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2372 2648 2782 3621 3729 3865 Size: 393 446 294 276 134 839 108 136 Cuts at: 3865 4112 4124 4148 4556 4705 4928 5120 5360 Size: 247 12 24 408 149 223 192 240 Cuts at: 5360 5371 5552 5732 5861 5993 6209 6371 6533 Size: 11 181 180 129 132 216 162 162 Cuts at: 6533 6616 6803 7130 7147 7268 7270 7449 7919 Size: 83 187 327 17 121 2 179 470 Cuts at: 7919 8091 8956 9323 9389 9401 9590 10121 10219 Size: 172 865 367 66 12 189 531 98 Cuts at: 10219 10307 10712 11138 11174 12080 12677 13464 13732 Size: 88 405 426 36 906 597 787 268 Cuts at: 13732 1239 Size: 1524 Fragments arranged by size: 1524 906 865 839 787 597 531 470 446 426 408 405 393 367 327 294 276 268 247 240 223 216 192 189 187 181 180 179 172 162 162 149 136 134 132 129 121 108 98 88 83 66 36 24 17 12 12 11 2 EcoNI CCTnn'n_nnAGG Cuts at: 2354 3759 4016 4538 4847 5198 5768 6107 6164 Size: 1405 257 522 309 351 570 339 57 Cuts at: 6164 6793 8942 10799 2354 Size: 629 2149 1857 5572 Fragments arranged by size: 5572 2149 1857 1405 629 570 522 351 339 309 257 57 EcoO109I rG'GnC_Cy Cuts at: 2420 2421 2705 2706 2802 3010 3060 3092 3586 Size: 1 284 1 96 208 50 32 494 Cuts at: 3586 3947 3959 4044 4099 4192 4283 4366 4438 Size: 361 12 85 55 93 91 83 72 Cuts at: 4438 5320 5836 5845 6046 6157 6220 6444 6925 Size: 882 516 9 201 111 63 224 481 Cuts at: 6925 7006 7050 7351 7360 7438 7457 8079 8498 Size: 81 44 301 9 78 19 622 419 Cuts at: 8498 8641 8642 8674 8691 8720 8721 8806 8814 Size: 143 1 32 17 29 1 85 8 Cuts at: 8814 9278 9538 9738 9882 10283 10831 11026 11165 Size: 464 260 200 144 401 548 195 139 Cuts at: 11165 11166 2420 Size: 1 5271 Fragments arranged by size: 5271 882 622 548 516 494 481 464 419 401 361 301 284 260 224 208 201 200 195 144 143 139 111 96 93 91 85 85 83 81 78 72 63 55 50 44 32 32 29 19 17 12 9 9 8 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 9857 10535 11326 11704 9857 Size: 678 791 378 12170 Fragments arranged by size: 12170 791 678 378 EcoRV GAT'ATC Cuts at: 7566 7566 Size: 14017 FseI GG_CCGG'CC Cuts at: 2078 2846 2078 Size: 768 13249 FspI TGC'GCA Cuts at: 8417 9491 12523 8417 Size: 1074 3032 9911 Fragments arranged by size: 9911 3032 1074 HaeII r_GCGC'y Cuts at: 1678 2048 2056 2350 2627 2883 3075 3975 4136 Size: 370 8 294 277 256 192 900 161 Cuts at: 4136 5374 6214 6481 6951 7028 7148 7256 7632 Size: 1238 840 267 470 77 120 108 376 Cuts at: 7632 7853 8786 9434 9751 10351 10752 13164 1678 Size: 221 933 648 317 600 401 2412 2531 Fragments arranged by size: 2531 2412 1238 933 900 840 648 600 470 401 376 370 317 294 277 267 256 221 192 161 120 108 77 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2084 2110 2116 2576 Size: 32 135 32 506 6 26 6 460 Cuts at: 2576 2608 2729 2761 3625 3657 5003 5035 5423 Size: 32 121 32 864 32 1346 32 388 Cuts at: 5423 5455 5566 5598 5771 5803 6274 6306 7178 Size: 32 111 32 173 32 471 32 872 Cuts at: 7178 7210 7934 7966 8015 8047 9011 9043 9725 Size: 32 724 32 49 32 964 32 682 Cuts at: 9725 9757 11028 11060 11072 11104 12658 12690 12732 Size: 32 1271 32 12 32 1554 32 42 Cuts at: 12732 12764 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 1346 1271 964 872 864 724 682 506 471 460 388 173 135 121 111 49 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 26 12 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 6229 10239 11185 11361 11607 669 Size: 988 281 4291 4010 946 176 246 3079 Fragments arranged by size: 4291 4010 3079 988 946 281 246 176 HindIII A'AGCT_T Cuts at: 748 5912 8538 748 Size: 5164 2626 6227 Fragments arranged by size: 6227 5164 2626 HpaI GTT'AAC Cuts at: 11607 11607 Size: 14017 KasI G'GCGC_C Cuts at: 2346 2879 3071 3971 4132 7252 7628 10347 2346 Size: 533 192 900 161 3120 376 2719 6016 Fragments arranged by size: 6016 3120 2719 900 533 376 192 161 KpnI G_GTAC'C Cuts at: 3216 5087 6325 11342 3216 Size: 1871 1238 5017 5891 Fragments arranged by size: 5891 5017 1871 1238 MfeI C'AATT_G Cuts at: 11616 11616 Size: 14017 MluI A'CGCG_T Cuts at: 13893 13893 Size: 14017 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 2766 2914 3879 9729 13131 13315 1305 Size: 861 600 148 965 5850 3402 184 2007 Fragments arranged by size: 5850 3402 2007 965 861 600 184 148 MscI TGG'CCA Cuts at: 10 64 1489 1832 4605 5142 5198 5736 6035 Size: 54 1425 343 2773 537 56 538 299 Cuts at: 6035 6841 8382 8579 8742 9563 10 Size: 806 1541 197 163 821 4464 Fragments arranged by size: 4464 2773 1541 1425 821 806 538 537 343 299 197 163 56 54 MslI CAynn'nnrTG Cuts at: 518 1166 4470 5092 5297 5589 7724 7769 8477 Size: 648 3304 622 205 292 2135 45 708 Cuts at: 8477 9149 11075 11975 12334 12493 518 Size: 672 1926 900 359 159 2042 Fragments arranged by size: 3304 2135 2042 1926 900 708 672 648 622 359 292 205 159 45 MspA1I CmG'CkG Cuts at: 1606 2148 2340 2755 2875 2960 3022 3300 3354 Size: 542 192 415 120 85 62 278 54 Cuts at: 3354 4984 5707 5752 6184 6352 6709 6871 7069 Size: 1630 723 45 432 168 357 162 198 Cuts at: 7069 7108 7216 7813 7918 8366 8654 9229 9301 Size: 39 108 597 105 448 288 575 72 Cuts at: 9301 9394 9700 10570 10858 10975 11131 11198 11433 Size: 93 306 870 288 117 156 67 235 Cuts at: 11433 12110 13258 13503 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1630 1148 870 723 677 597 575 542 448 432 415 357 306 288 288 278 245 235 198 192 168 162 156 120 117 108 105 93 85 72 67 62 54 45 39 NaeI GCC'GGC Cuts at: 2076 2844 9639 10352 10499 10643 2076 Size: 768 6795 713 147 144 5450 Fragments arranged by size: 6795 5450 768 713 147 144 NarI GG'CG_CC Cuts at: 2347 2880 3072 3972 4133 7253 7629 10348 2347 Size: 533 192 900 161 3120 376 2719 6016 Fragments arranged by size: 6016 3120 2719 900 533 376 192 161 NcoI C'CATG_G Cuts at: 513 1065 2022 5587 7659 7764 8377 513 Size: 552 957 3565 2072 105 613 6153 Fragments arranged by size: 6153 3565 2072 957 613 552 105 NdeI CA'TA_TG Cuts at: 387 387 Size: 14017 NgoMIV G'CCGG_C Cuts at: 2074 2842 9637 10350 10497 10641 2074 Size: 768 6795 713 147 144 5450 Fragments arranged by size: 6795 5450 768 713 147 144 NheI G'CTAG_C Cuts at: 1052 10837 1052 Size: 9785 4232 NotI GC'GGCC_GC Cuts at: 2335 2389 3188 11464 2335 Size: 54 799 8276 4888 Fragments arranged by size: 8276 4888 799 54 NruI TCG'CGA Cuts at: 3056 5152 3056 Size: 2096 11921 NsiI A_TGCA'T Cuts at: 4448 4448 Size: 14017 NspI r_CATG'y Cuts at: 1132 1229 5942 6298 8482 11023 11375 12920 1132 Size: 97 4713 356 2184 2541 352 1545 2229 Fragments arranged by size: 4713 2541 2229 2184 1545 356 352 97 PasI CC'CwG_GG Cuts at: 1348 2261 2328 2710 3142 6049 7011 8188 8810 Size: 913 67 382 432 2907 962 1177 622 Cuts at: 8810 1348 Size: 6555 Fragments arranged by size: 6555 2907 1177 962 913 622 432 382 67 PciI A'CATG_T Cuts at: 5938 12916 5938 Size: 6978 7039 PflMI CCAn_nnn'nTGG Cuts at: 1108 2056 2267 3088 5029 5755 6049 6115 6223 Size: 948 211 821 1941 726 294 66 108 Cuts at: 6223 6874 8128 10297 1108 Size: 651 1254 2169 4828 Fragments arranged by size: 4828 2169 1941 1254 948 821 726 651 294 211 108 66 PfoI T'CCnGG_A Cuts at: 2798 5520 5769 6198 7539 9966 2798 Size: 2722 249 429 1341 2427 6849 Fragments arranged by size: 6849 2722 2427 1341 429 249 PmeI GTTT'AAAC Cuts at: 11321 11321 Size: 14017 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10865 10897 12086 12118 13599 13631 10865 Size: 32 1189 32 1481 32 11251 Fragments arranged by size: 11251 1481 1189 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2802 3010 3586 3959 4044 4099 4192 4438 5845 Size: 208 576 373 85 55 93 246 1407 Cuts at: 5845 6157 7351 7360 7457 8806 8814 9538 9882 Size: 312 1194 9 97 1349 8 724 344 Cuts at: 9882 10831 2802 Size: 949 5988 Fragments arranged by size: 5988 1407 1349 1194 949 724 576 373 344 312 246 208 97 93 85 55 9 8 PshAI GACnn'nnGTC Cuts at: 1613 2748 3342 1613 Size: 1135 594 12288 Fragments arranged by size: 12288 1135 594 PsiI TTA'TAA Cuts at: 11587 11587 Size: 14017 PspOMI G'GGCC_C Cuts at: 2324 2420 2705 3091 6924 7089 7637 8497 8641 Size: 96 285 386 3833 165 548 860 144 Cuts at: 8641 8690 8720 11165 2324 Size: 49 30 2445 5176 Fragments arranged by size: 5176 3833 2445 860 548 386 285 165 144 96 49 30 PspXI vC'TCGA_Gb Cuts at: 2746 10191 2746 Size: 7445 6572 PstI C_TGCA'G Cuts at: 830 2236 2255 4017 4472 4821 4865 4920 4983 Size: 1406 19 1762 455 349 44 55 63 Cuts at: 4983 6168 6408 7236 9515 10275 10587 10791 11130 Size: 1185 240 828 2279 760 312 204 339 Cuts at: 11130 11369 830 Size: 239 3478 Fragments arranged by size: 3478 2279 1762 1406 1185 828 760 455 349 339 312 240 239 204 63 55 44 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 14017 PvuII CAG'CTG Cuts at: 4984 5707 5752 6352 6709 6871 7069 7108 7216 Size: 723 45 600 357 162 198 39 108 Cuts at: 7216 7813 8654 9229 9301 9394 9700 10570 10858 Size: 597 841 575 72 93 306 870 288 Cuts at: 10858 10975 11198 4984 Size: 117 223 7803 Fragments arranged by size: 7803 870 841 723 600 597 575 357 306 288 223 198 162 117 108 93 72 45 39 SacI G_AGCT'C Cuts at: 6198 6861 6198 Size: 663 13354 SacII CC_GC'GG Cuts at: 2149 2341 3301 3355 2149 Size: 192 960 54 12811 Fragments arranged by size: 12811 960 192 54 SalI G'TCGA_C Cuts at: 1936 6227 10237 11359 1936 Size: 4291 4010 1122 4594 Fragments arranged by size: 4594 4291 4010 1122 SanDI GG'GwC_CC Cuts at: 4044 7360 8814 4044 Size: 3316 1454 9247 Fragments arranged by size: 9247 3316 1454 SapI GCTCTTCn'nnn_ Cuts at: 2373 5387 5567 9377 10556 11114 2373 Size: 3014 180 3810 1179 558 5276 Fragments arranged by size: 5276 3810 3014 1179 558 180 SbfI CC_TGCA'GG Cuts at: 4821 4865 11369 4821 Size: 44 6504 7469 Fragments arranged by size: 7469 6504 44 ScaI AGT'ACT Cuts at: 12265 13977 12265 Size: 1712 12305 SexAI A'CCwGG_T Cuts at: 2928 5670 6816 2928 Size: 2742 1146 10129 Fragments arranged by size: 10129 2742 1146 SfcI C'TryA_G Cuts at: 826 1045 2232 2251 4013 4468 4667 4817 4861 Size: 219 1187 19 1762 455 199 150 44 Cuts at: 4861 4916 4979 5375 6164 6404 6971 7232 8681 Size: 55 63 396 789 240 567 261 1449 Cuts at: 8681 9511 10011 10271 10583 10702 10787 11126 11365 Size: 830 500 260 312 119 85 339 239 Cuts at: 11365 12500 13181 13372 826 Size: 1135 681 191 1471 Fragments arranged by size: 1762 1471 1449 1187 1135 830 789 681 567 500 455 396 339 312 261 260 240 239 219 199 191 150 119 85 63 55 44 19 SfiI GGCCn_nnn'nGGCC Cuts at: 2071 2909 6032 7079 7088 2071 Size: 838 3123 1047 9 9000 Fragments arranged by size: 9000 3123 1047 838 9 SfoI GGC'GCC Cuts at: 2348 2881 3073 3973 4134 7254 7630 10349 2348 Size: 533 192 900 161 3120 376 2719 6016 Fragments arranged by size: 6016 3120 2719 900 533 376 192 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 14017 SgrAI Cr'CCGG_yG Cuts at: 1700 10350 1700 Size: 8650 5367 SmaI CCC'GGG Cuts at: 1872 2068 2555 2678 2915 3496 4032 7262 8496 Size: 196 487 123 237 581 536 3230 1234 Cuts at: 8496 9413 10064 11344 1872 Size: 917 651 1280 4545 Fragments arranged by size: 4545 3230 1280 1234 917 651 581 536 487 237 196 123 SmlI C'TyrA_G Cuts at: 820 1017 1943 2746 3110 4241 4712 6986 9263 Size: 197 926 803 364 1131 471 2274 2277 Cuts at: 9263 9458 10191 12121 13022 13284 820 Size: 195 733 1930 901 262 1553 Fragments arranged by size: 2277 2274 1930 1553 1131 926 901 803 733 471 364 262 197 195 SnaBI TAC'GTA Cuts at: 493 11314 11808 493 Size: 10821 494 2702 Fragments arranged by size: 10821 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 14017 SphI G_CATG'C Cuts at: 1132 6298 11023 11375 1132 Size: 5166 4725 352 3774 Fragments arranged by size: 5166 4725 3774 352 SrfI GCCC'GGGC Cuts at: 2915 3496 8496 9413 2915 Size: 581 5000 917 7519 Fragments arranged by size: 7519 5000 917 581 SspI AAT'ATT Cuts at: 5 52 11941 5 Size: 47 11889 2081 Fragments arranged by size: 11889 2081 47 StuI AGG'CCT Cuts at: 1517 2903 4859 5922 6026 7245 7958 8530 9890 Size: 1386 1956 1063 104 1219 713 572 1360 Cuts at: 9890 1517 Size: 5644 Fragments arranged by size: 5644 1956 1386 1360 1219 1063 713 572 104 StyI C'CwwG_G Cuts at: 513 1065 2022 2847 3962 3999 4152 5199 5587 Size: 552 957 825 1115 37 153 1047 388 Cuts at: 5587 6142 6153 6447 7053 7659 7764 8172 8355 Size: 555 11 294 606 606 105 408 183 Cuts at: 8355 8370 8377 8383 8395 8562 8670 9274 9666 Size: 15 7 6 12 167 108 604 392 Cuts at: 9666 9761 9954 10752 11456 513 Size: 95 193 798 704 3074 Fragments arranged by size: 3074 1115 1047 957 825 798 704 606 606 604 555 552 408 392 388 294 193 183 167 153 108 105 95 37 15 12 11 7 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5434 7212 7732 8888 9329 10034 10712 Size: 1255 3854 1778 520 1156 441 705 678 Cuts at: 10712 12044 12061 12214 12399 12844 325 Size: 1332 17 153 185 445 1498 Fragments arranged by size: 3854 1778 1498 1332 1255 1156 705 678 520 445 441 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 9948 11100 12263 Size: 274 80 33 51 1443 7971 1152 1163 Cuts at: 12263 13975 96 Size: 1712 138 Fragments arranged by size: 7971 1712 1443 1163 1152 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2928 2960 4323 4355 4680 4712 5333 5365 5405 Size: 32 1363 32 325 32 621 32 40 Cuts at: 5405 5437 6951 6983 9943 9975 10478 10510 13977 Size: 32 1514 32 2960 32 503 32 3467 Cuts at: 13977 14009 2928 Size: 32 2936 Fragments arranged by size: 3467 2960 2936 1514 1363 621 503 325 40 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 9131 1316 Size: 7815 6202 XbaI T'CTAG_A Cuts at: 11353 11353 Size: 14017 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2207 4981 5191 5251 5585 6367 7762 9430 10294 Size: 2774 210 60 334 782 1395 1668 864 Cuts at: 10294 2207 Size: 5930 Fragments arranged by size: 5930 2774 1668 1395 864 782 334 210 60 XhoI C'TCGA_G Cuts at: 1943 2746 10191 1943 Size: 803 7445 5769 Fragments arranged by size: 7445 5769 803 XmaI C'CCGG_G Cuts at: 1870 2066 2553 2676 2913 3494 4030 7260 8494 Size: 196 487 123 237 581 536 3230 1234 Cuts at: 8494 9411 10062 11342 1870 Size: 917 651 1280 4545 Fragments arranged by size: 4545 3230 1280 1234 917 651 581 536 487 237 196 123 XmnI GAAnn'nnTTC Cuts at: 8117 8933 10382 12146 8117 Size: 816 1449 1764 9988 Fragments arranged by size: 9988 1764 1449 816 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 11825 276 Size: 53 83 186 936 10291 2468 Fragments arranged by size: 10291 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: BaeI BsiWI BstZ17I FalI FspAI PacI PmlI PsrI RsrII SwaI