Restriction map for
pFN21AA1780
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BlnI BseAI BsiEI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 2523 CTTAAG C^TTAAG
AocI 1066 CCTGAGG CC^T[ACGT]AGG
AspI 610 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 805 CCCGAG C^[CT]CG[GA]G
BamHI 3288 GGATCC G^GATCC
BbeI 1957 GGCGCC GGCGC^C
BbvCI 1717 GCTGAGG CCTCAGC(-5/-2)
BcgI 2973 CGAGCTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2523 CTTAAG C^TTAAG
BlpI 1615 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1615 GCTGAGC GC^T[ACGT]AGC
BsaBI 418 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 964 GAGACC GGTCTC(1/5)
BsaWI 649 ACCGGA [AT]^CCGG[AT]
BseRI 728 GAGGAG GAGGAG(10/8)
BsiWI 1598 CGTACG C^GTACG
BsmBI 3277 CGTCTC CGTCTC(1/5)
BsoBI 805 CCCGAG C^[CT]CG[GA]G
BspLU11I 1195 ACATGT A^CATGT
BsrBI 1005 GAGCGG CCGCTC(-3/-3)
BssSI 171 CACGAG CACGAG(-5/-1)
Bsu36I 1066 CCTGAGG CC^T[ACGT]AGG
CelII 1615 GCTGAGC GC^T[ACGT]AGC
DraI 199 TTTAAA TTT^AAA
Eco81I 1066 CCTGAGG CC^T[ACGT]AGG
EcoRI 3036 GAATTC G^AATTC
HindIII 1220 AAGCTT A^AGCTT
KasI 1957 GGCGCC G^GCGCC
MamI 418 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1957 GGCGCC GG^CGCC
NcoI 342 CCATGG C^CATGG
PflFI 610 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1239 CAGCTG CAG^CTG
SacI 2974 GAGCTC GAGCT^C
ScaI 2536 AGTACT AGT^ACT
SfoI 1957 GGCGCC GGC^GCC
SmlI 2523 CTTAAG C^T[CT][GA]AG
TaqI 240 TCGA T^CGA
Tth111I 610 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 240 TCGA T^CGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 209 CGCG CG^CG
1561 CGCG
AcyI 1957 GGCGCC G[GA]^CG[CT]C
3275 GGCGTC
Aor51HI 1043 AGCGCT AGC^GCT
1619 AGCGCT
Asp700I 3032 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3167 GAAGAGATTC
BbsI 85 GAAGAC GAAGAC(2/6)
252 GAAGAC
BciVI 643 GGATAC GTATCC(6/5)
901 GGATAC
BfaI 2242 CTAG C^TAG
2599 CTAG
BglI 764 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1586 GCCAGGATGGC
BglII 1232 AGATCT A^GATCT
2056 AGATCT
BpuAI 85 GAAGAC GAAGAC(2/6)
252 GAAGAC
BsaHI 1957 GGCGCC G[GA]^CG[CT]C
3275 GGCGTC
BstAPI 1027 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2087 GCAGTGACTGC
BstUI 209 CGCG CG^CG
1561 CGCG
DsaI 342 CCATGG C^C[GA][CT]GG
1658 CCACGG
Eco47III 1043 AGCGCT AGC^GCT
1619 AGCGCT
EcoT14I 342 CCATGG C^C[AT][AT]GG
3252 CCAAGG
HgaI 1724 GACGC GACGC(5/10)
3276 GCGTC
Hin1I 1957 GGCGCC G[GA]^CG[CT]C
3275 GGCGTC
HincII 979 GTCAAC GT[CT]^[GA]AC
2824 GTCAAC
HindII 979 GTCAAC GT[CT]^[GA]AC
2824 GTCAAC
MaeI 2242 CTAG C^TAG
2599 CTAG
MvnI 209 CGCG CG^CG
1561 CGCG
NaeI 2940 GCCGGC GCC^GGC
3159 GCCGGC
NgoMIV 2940 GCCGGC G^CCGGC
3159 GCCGGC
SphI 1012 GCATGC GCATG^C
2666 GCATGC
StyI 342 CCATGG C^C[AT][AT]GG
3252 CCAAGG
XmnI 3032 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3167 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AvaII BanI BanII
BbrPI BbvI BclI BcnI BmyI
BpmI BsaAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII EarI EcoO109I
EcoRII FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII