(Circular) (Six-base) MAPSORT of: pFN21AA1780.seq Check: 6570 from: 1 to: 8125 pFN21AA1780 8125 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2618 4126 4439 2618 Size: 1508 313 6304 Fragments arranged by size: 6304 1508 313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5935 278 Size: 53 83 186 936 4399 2468 Fragments arranged by size: 4399 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5468 1937 Size: 3531 4594 Acc65I G'GTAC_C Cuts at: 5446 5446 Size: 8125 AclI AA'CG_TT Cuts at: 1563 6252 6625 1563 Size: 4689 373 3063 Fragments arranged by size: 4689 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4359 5193 6188 7572 7840 1632 Size: 2727 834 995 1384 268 1917 Fragments arranged by size: 2727 1917 1384 995 834 268 AfeI AGC'GCT Cuts at: 3050 3626 3050 Size: 576 7549 AflII C'TTAA_G Cuts at: 820 1017 4528 820 Size: 197 3511 4417 Fragments arranged by size: 4417 3511 197 AflIII A'CryG_T Cuts at: 1203 2867 3200 3548 4146 7024 8001 1203 Size: 1664 333 348 598 2878 977 1327 Fragments arranged by size: 2878 1664 1327 977 598 348 333 AgeI A'CCGG_T Cuts at: 6956 6956 Size: 8125 AhdI GACnn_n'nnGTC Cuts at: 6854 6854 Size: 8125 AleI CACnn'nnGTG Cuts at: 1166 3064 1166 Size: 1898 6227 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3667 4294 1184 Size: 2483 627 5015 Fragments arranged by size: 5015 2483 627 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3343 3375 3343 Size: 32 8093 AlwNI CAG_nnn'CTG Cuts at: 2132 2418 4098 5868 7440 2132 Size: 286 1680 1770 1572 2817 Fragments arranged by size: 2817 1770 1680 1572 286 ApaI G_GGCC'C Cuts at: 3740 3915 4133 3740 Size: 175 218 7732 Fragments arranged by size: 7732 218 175 ApaLI G'TGCA_C Cuts at: 3286 3552 3810 4269 6182 7338 3286 Size: 266 258 459 1913 1156 4073 Fragments arranged by size: 4073 1913 1156 459 266 258 ApoI r'AATT_y Cuts at: 1493 2166 4017 5041 5434 5660 5812 7921 1493 Size: 673 1851 1024 393 226 152 2109 1697 Fragments arranged by size: 2109 1851 1697 1024 673 393 226 152 AseI AT'TA_AT Cuts at: 160 1032 6679 160 Size: 872 5647 1606 Fragments arranged by size: 5647 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8125 AvaI C'yCGr_G Cuts at: 1870 1943 2810 5450 1870 Size: 73 867 2640 4545 Fragments arranged by size: 4545 2640 867 73 BamHI G'GATC_C Cuts at: 5293 5455 5822 5293 Size: 162 367 7596 Fragments arranged by size: 7596 367 162 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2457 2558 2922 3827 3962 Size: 325 217 627 670 101 364 905 135 Cuts at: 3962 5446 6901 618 Size: 1484 1455 1842 Fragments arranged by size: 1842 1484 1455 905 670 627 364 325 217 135 101 BanII G_rGCy'C Cuts at: 1391 2479 3081 3740 3915 4133 4224 4983 5444 Size: 1088 602 659 175 218 91 759 461 Cuts at: 5444 1391 Size: 4072 Fragments arranged by size: 4072 1088 759 659 602 461 218 175 91 BbeI G_GCGC'C Cuts at: 3966 3966 Size: 8125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 2264 928 Size: 971 198 167 6789 Fragments arranged by size: 6789 971 198 167 BbvCI CC'TCA_GC Cuts at: 3723 3723 Size: 8125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4967 5001 6316 6350 1903 Size: 34 3030 34 1315 34 3678 Fragments arranged by size: 3678 3030 1315 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2642 2900 6017 7227 2642 Size: 258 3117 1210 3540 Fragments arranged by size: 3540 3117 1210 258 BclI T'GATC_A Cuts at: 1548 2161 2428 4489 1548 Size: 613 267 2061 5184 Fragments arranged by size: 5184 2061 613 267 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2775 3597 6736 Size: 107 122 71 631 605 1103 822 3139 Cuts at: 6736 136 Size: 1525 Fragments arranged by size: 3139 1525 1103 822 631 605 122 107 71 BglII A'GATC_T Cuts at: 3237 4061 8120 3237 Size: 824 4059 3242 Fragments arranged by size: 4059 3242 824 BlpI GC'TnA_GC Cuts at: 3621 5542 3621 Size: 1921 6204 Bme1580I G_kGCm'C Cuts at: 1790 2561 2789 3290 3298 3556 3740 3814 3915 Size: 771 228 501 8 258 184 74 101 Cuts at: 3915 4133 4273 4373 6186 7342 1790 Size: 218 140 100 1813 1156 2573 Fragments arranged by size: 2573 1813 1156 771 501 258 228 218 184 140 101 100 74 8 BmgBI CAC'GTC Cuts at: 1318 2620 2749 2868 4828 1318 Size: 1302 129 119 1960 4615 Fragments arranged by size: 4615 1960 1302 129 119 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2122 2443 3459 Size: 416 53 83 399 24 705 321 1016 Cuts at: 3459 3981 4135 4455 4782 4801 4944 5856 6814 Size: 522 154 320 327 19 143 912 958 Cuts at: 6814 442 Size: 1753 Fragments arranged by size: 1753 1016 958 912 705 522 416 399 327 321 320 154 143 83 53 24 19 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8125 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4260 4260 Size: 8125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2395 2775 3171 6785 1239 Size: 1156 380 396 3614 2579 Fragments arranged by size: 3614 2579 1156 396 380 Bpu10I CC'TnA_GC Cuts at: 2030 3723 5072 5373 2030 Size: 1693 1349 301 4782 Fragments arranged by size: 4782 1693 1349 301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6250 7115 7413 6250 Size: 865 298 6962 Fragments arranged by size: 6962 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2963 6788 882 Size: 617 1464 3825 2219 Fragments arranged by size: 3825 2219 1464 617 BsaAI yAC'GTr Cuts at: 493 1204 3007 3549 3603 4149 5916 493 Size: 711 1803 542 54 546 1767 2702 Fragments arranged by size: 2702 1803 1767 711 546 542 54 BsaBI GATnn'nnATC Cuts at: 2427 8119 2427 Size: 5692 2433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3963 5281 5932 6314 Size: 53 83 186 936 2430 1318 651 382 Cuts at: 6314 6949 275 Size: 635 1451 Fragments arranged by size: 2430 1451 1318 936 651 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2654 6558 6956 7230 7377 7788 2654 Size: 3904 398 274 147 411 2991 Fragments arranged by size: 3904 2991 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3190 3220 1178 Size: 30 165 30 1787 30 6083 Fragments arranged by size: 6083 1787 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2748 1186 Size: 1562 6563 BseYI C'CCAG_C Cuts at: 2391 3732 7328 7824 2391 Size: 1341 3596 496 2692 Fragments arranged by size: 3596 2692 1341 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2167 2431 3529 4012 4032 4213 4915 Size: 573 431 264 1098 483 20 181 702 Cuts at: 4915 1163 Size: 4373 Fragments arranged by size: 4373 1098 702 573 483 431 264 181 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5575 6336 7364 1540 Size: 275 186 3574 761 1028 2301 Fragments arranged by size: 3574 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3290 3556 3814 4273 4983 6186 6271 7342 3290 Size: 266 258 459 710 1203 85 1071 4073 Fragments arranged by size: 4073 1203 1071 710 459 266 258 85 BsiWI C'GTAC_G Cuts at: 3603 3603 Size: 8125 BsmI GAATG_Cn' Cuts at: 2636 2765 2849 5636 5729 2636 Size: 129 84 2787 93 5032 Fragments arranged by size: 5032 2787 129 93 84 BsmBI CGTCTCn'nnnn_ Cuts at: 5288 5288 Size: 8125 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2479 2561 2789 3081 3290 3298 3556 Size: 399 689 82 228 292 209 8 258 Cuts at: 3556 3740 3814 3915 4133 4224 4273 4373 4983 Size: 184 74 101 218 91 49 100 610 Cuts at: 4983 5444 6186 6271 7342 1391 Size: 461 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 689 610 461 399 292 258 228 218 209 184 101 100 91 85 82 74 49 8 BspEI T'CCGG_A Cuts at: 7788 7788 Size: 8125 BspHI T'CATG_A Cuts at: 6014 7744 6014 Size: 1730 6395 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2618 2981 3551 4034 4126 4439 5349 5480 Size: 1774 363 570 483 92 313 910 131 Cuts at: 5480 844 Size: 3489 Fragments arranged by size: 3489 1774 910 570 483 363 313 131 92 BsrBI CCG'CTC Cuts at: 1394 1823 3012 6012 1394 Size: 429 1189 3000 3507 Fragments arranged by size: 3507 3000 1189 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2893 3670 3979 4578 4756 4940 6620 Size: 1183 1645 777 309 599 178 184 1680 Cuts at: 6620 6794 65 Size: 174 1396 Fragments arranged by size: 1680 1645 1396 1183 777 599 309 184 178 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2454 3929 4945 5164 6769 6956 1700 Size: 110 644 1475 1016 219 1605 187 2869 Fragments arranged by size: 2869 1605 1475 1016 644 219 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8125 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8125 BssSI C'ACGA_G Cuts at: 2176 6185 7197 2176 Size: 4009 1012 3104 Fragments arranged by size: 4009 3104 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3038 4098 794 Size: 2244 1060 4821 Fragments arranged by size: 4821 2244 1060 BstBI TT'CG_AA Cuts at: 6929 6929 Size: 8125 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8125 BstXI CCAn_nnnn'nTGG Cuts at: 2063 3647 4316 4445 2063 Size: 1584 669 129 5743 Fragments arranged by size: 5743 1584 669 129 BstYI r'GATC_y Cuts at: 1972 2671 3237 3843 4061 5293 5455 5822 6207 Size: 699 566 606 218 1232 162 367 385 Cuts at: 6207 6224 7676 7762 7774 8120 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1232 699 606 566 385 367 346 218 162 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3072 3072 Size: 8125 BtgI C'CryG_G Cuts at: 513 1065 2004 2347 3663 513 Size: 552 939 343 1316 4975 Fragments arranged by size: 4975 1316 939 552 343 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2436 3854 487 Size: 683 56 1210 1418 4758 Fragments arranged by size: 4758 1418 1210 683 56 BtsI GCAGTG_nn' Cuts at: 2443 4099 4328 5425 5641 6435 6455 2443 Size: 1656 229 1097 216 794 20 4113 Fragments arranged by size: 4113 1656 1097 794 229 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8125 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8090 DraI TTT'AAA Cuts at: 2206 5429 2206 Size: 3223 4902 DraIII CAC_nnn'GTG Cuts at: 2705 2793 3442 4809 2705 Size: 88 649 1367 6021 Fragments arranged by size: 6021 1367 649 88 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7132 809 Size: 507 5816 1802 Fragments arranged by size: 5816 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5572 6461 8090 Size: 54 1425 50 275 18 3742 889 1629 Cuts at: 8090 8 Size: 43 Fragments arranged by size: 3742 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5572 1537 Size: 275 3760 4090 Fragments arranged by size: 4090 3760 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4619 4904 5167 6055 1353 Size: 3266 285 263 888 3423 Fragments arranged by size: 3423 3266 888 285 263 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6712 7086 7232 387 Size: 1064 138 5123 374 146 1280 Fragments arranged by size: 5123 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4981 4981 Size: 8125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2395 2775 3171 4359 5193 6188 6785 Size: 393 763 380 396 1188 834 995 597 Cuts at: 6785 7572 7840 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1188 995 834 787 763 597 396 393 380 268 EcoO109I rG'GnC_Cy Cuts at: 2438 2946 3540 3736 3737 3912 4129 4449 4916 Size: 508 594 196 1 175 217 320 467 Cuts at: 4916 2438 Size: 5647 Fragments arranged by size: 5647 594 508 467 320 217 196 175 1 EcoRI G'AATT_C Cuts at: 5041 5434 5812 5041 Size: 393 378 7354 Fragments arranged by size: 7354 393 378 FspI TGC'GCA Cuts at: 6631 6631 Size: 8125 HaeII r_GCGC'y Cuts at: 1678 2536 3052 3628 3966 4182 7272 1678 Size: 858 516 576 338 216 3090 2531 Fragments arranged by size: 3090 2531 858 576 516 338 216 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2259 3190 3222 4474 Size: 32 135 32 655 32 931 32 1252 Cuts at: 4474 4506 5144 5176 5348 5380 6766 6798 6840 Size: 32 638 32 172 32 1386 32 42 Cuts at: 6840 6872 1373 Size: 32 2626 Fragments arranged by size: 2626 1386 1252 931 655 638 172 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 4831 5469 5715 669 Size: 988 281 1048 1845 638 246 3079 Fragments arranged by size: 3079 1845 1048 988 638 281 246 HindIII A'AGCT_T Cuts at: 748 3225 748 Size: 2477 5648 HpaI GTT'AAC Cuts at: 5715 5715 Size: 8125 KasI G'GCGC_C Cuts at: 3962 3962 Size: 8125 KpnI G_GTAC'C Cuts at: 5450 5450 Size: 8125 MfeI C'AATT_G Cuts at: 5724 5724 Size: 8125 MluI A'CGCG_T Cuts at: 8001 8001 Size: 8125 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4064 4492 7239 7423 1305 Size: 2759 428 2747 184 2007 Fragments arranged by size: 2759 2747 2007 428 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6303 Fragments arranged by size: 6303 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3064 3502 3559 3645 4276 4749 6083 Size: 648 1898 438 57 86 631 473 1334 Cuts at: 6083 6442 6601 518 Size: 359 159 2042 Fragments arranged by size: 2042 1898 1334 648 631 473 438 359 159 86 57 MspA1I CmG'CkG Cuts at: 1606 2547 2637 3246 3678 3909 5541 6218 7366 Size: 941 90 609 432 231 1632 677 1148 Cuts at: 7366 7611 1606 Size: 245 2120 Fragments arranged by size: 2120 1632 1148 941 677 609 432 245 231 90 NaeI GCC'GGC Cuts at: 4947 5166 4947 Size: 219 7906 NarI GG'CG_CC Cuts at: 3963 3963 Size: 8125 NcoI C'CATG_G Cuts at: 513 1065 2004 2347 513 Size: 552 939 343 6291 Fragments arranged by size: 6291 939 552 343 NdeI CA'TA_TG Cuts at: 387 4384 5092 5185 5299 387 Size: 3997 708 93 114 3213 Fragments arranged by size: 3997 3213 708 114 93 NgoMIV G'CCGG_C Cuts at: 4945 5164 4945 Size: 219 7906 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8125 NotI GC'GGCC_GC Cuts at: 5572 5572 Size: 8125 NspI r_CATG'y Cuts at: 1132 1229 3021 3204 3501 3888 4675 5483 7028 Size: 97 1792 183 297 387 787 808 1545 Cuts at: 7028 1132 Size: 2229 Fragments arranged by size: 2229 1792 1545 808 787 387 297 183 97 PasI CC'CwG_GG Cuts at: 1348 4904 1348 Size: 3556 4569 PciI A'CATG_T Cuts at: 3200 7024 3200 Size: 3824 4301 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8125 PfoI T'CCnGG_A Cuts at: 2290 2902 3208 2290 Size: 612 306 7207 Fragments arranged by size: 7207 612 306 PmeI GTTT'AAAC Cuts at: 5429 5429 Size: 8125 PmlI CAC'GTG Cuts at: 3007 3549 4149 3007 Size: 542 600 6983 Fragments arranged by size: 6983 600 542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4726 4758 6194 6226 7707 7739 4726 Size: 32 1436 32 1481 32 5112 Fragments arranged by size: 5112 1481 1436 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2438 2946 3540 4449 2438 Size: 508 594 909 6114 Fragments arranged by size: 6114 909 594 508 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8125 PsiI TTA'TAA Cuts at: 5695 5695 Size: 8125 PspOMI G'GGCC_C Cuts at: 3736 3911 4129 3736 Size: 175 218 7732 Fragments arranged by size: 7732 218 175 PstI C_TGCA'G Cuts at: 830 3245 3638 3878 4178 4463 5477 830 Size: 2415 393 240 300 285 1014 3478 Fragments arranged by size: 3478 2415 1014 393 300 285 240 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8125 PvuII CAG'CTG Cuts at: 3246 3246 Size: 8125 SacI G_AGCT'C Cuts at: 4983 4983 Size: 8125 SalI G'TCGA_C Cuts at: 1936 5467 1936 Size: 3531 4594 SanDI GG'GwC_CC Cuts at: 2438 2438 Size: 8125 SbfI CC_TGCA'GG Cuts at: 5477 5477 Size: 8125 ScaI AGT'ACT Cuts at: 4543 6373 8085 4543 Size: 1830 1712 4583 Fragments arranged by size: 4583 1830 1712 SfcI C'TryA_G Cuts at: 826 1045 3241 3634 3874 4174 4459 5241 5473 Size: 219 2196 393 240 300 285 782 232 Cuts at: 5473 6608 7289 7480 826 Size: 1135 681 191 1471 Fragments arranged by size: 2196 1471 1135 782 681 393 300 285 240 232 219 191 SfoI GGC'GCC Cuts at: 3964 3964 Size: 8125 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8125 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8125 SmaI CCC'GGG Cuts at: 1872 5452 1872 Size: 3580 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4528 6229 7130 7392 820 Size: 197 926 2585 1701 901 262 1553 Fragments arranged by size: 2585 1701 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5916 493 Size: 5423 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8125 SphI G_CATG'C Cuts at: 1132 3021 4675 5483 1132 Size: 1889 1654 808 3774 Fragments arranged by size: 3774 1889 1654 808 SspI AAT'ATT Cuts at: 5 52 6049 5 Size: 47 5997 2081 Fragments arranged by size: 5997 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8125 StyI C'CwwG_G Cuts at: 513 1065 2004 2347 5257 5564 513 Size: 552 939 343 2910 307 3074 Fragments arranged by size: 3074 2910 939 552 343 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4820 6152 6169 6322 6507 6952 325 Size: 1255 3240 1332 17 153 185 445 1498 Fragments arranged by size: 3240 1498 1332 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3416 4325 4541 Size: 274 80 33 51 1443 1439 909 216 Cuts at: 4541 5116 6371 8083 96 Size: 575 1255 1712 138 Fragments arranged by size: 1712 1443 1439 1255 909 575 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3804 3836 3994 4026 4078 4110 8085 8117 3804 Size: 32 158 32 52 32 3975 32 3812 Fragments arranged by size: 3975 3812 158 52 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2618 1316 Size: 1302 6823 XbaI T'CTAG_A Cuts at: 5461 5461 Size: 8125 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2468 2597 2855 4122 2468 Size: 129 258 1267 6471 Fragments arranged by size: 6471 1267 258 129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8125 XmaI C'CCGG_G Cuts at: 1870 5450 1870 Size: 3580 4545 XmnI GAAnn'nnTTC Cuts at: 5041 5176 6254 5041 Size: 135 1078 6912 Fragments arranged by size: 6912 1078 135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5933 276 Size: 53 83 186 936 4399 2468 Fragments arranged by size: 4399 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI