Restriction map for
pFN21AA1780
(Linear) (Six-base) MAPSORT of: KIAA1780.seq Check: 4393 from: 1 to: 3420
KIAA1780.orf
With 182 enzymes: SgfI *
May 30, 2008 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 613 2121 2434 3420
Size: 613 1508 313 986
Fragments arranged by size:
1508 986 613 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2354 3188 3420
Size: 2354 834 232
Fragments arranged by size:
2354 834 232
AfeI AGC'GCT
Cuts at: 0 1045 1621 3420
Size: 1045 576 1799
Fragments arranged by size:
1799 1045 576
AflII C'TTAA_G
Cuts at: 0 2523 3420
Size: 2523 897
AflIII A'CryG_T
Cuts at: 0 862 1195 1543 2141 3420
Size: 862 333 348 598 1279
Fragments arranged by size:
1279 862 598 348 333
AleI CACnn'nnGTG
Cuts at: 0 1059 3420
Size: 1059 2361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1662 2289 3420
Size: 1662 627 1131
Fragments arranged by size:
1662 1131 627
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1338 1370 3420
Size: 1338 32 2050
Fragments arranged by size:
2050 1338 32
AlwNI CAG_nnn'CTG
Cuts at: 0 127 413 2093 3420
Size: 127 286 1680 1327
Fragments arranged by size:
1680 1327 286 127
ApaI G_GGCC'C
Cuts at: 0 1735 1910 2128 3420
Size: 1735 175 218 1292
Fragments arranged by size:
1735 1292 218 175
ApaLI G'TGCA_C
Cuts at: 0 1281 1547 1805 2264 3420
Size: 1281 266 258 459 1156
Fragments arranged by size:
1281 1156 459 266 258
ApoI r'AATT_y
Cuts at: 0 161 2012 3036 3420
Size: 161 1851 1024 384
Fragments arranged by size:
1851 1024 384 161
AvaI C'yCGr_G
Cuts at: 0 805 3420
Size: 805 2615
BamHI G'GATC_C
Cuts at: 0 3288 3420
Size: 3288 132
BanI G'GyrC_C
Cuts at: 0 452 553 917 1822 1957 3420
Size: 452 101 364 905 135 1463
Fragments arranged by size:
1463 905 452 364 135 101
BanII G_rGCy'C
Cuts at: 0 474 1076 1735 1910 2128 2219 2978 3420
Size: 474 602 659 175 218 91 759 442
Fragments arranged by size:
759 659 602 474 442 218 175 91
BbeI G_GCGC'C
Cuts at: 0 1961 3420
Size: 1961 1459
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 259 3420
Size: 92 167 3161
Fragments arranged by size:
3161 167 92
BbvCI CC'TCA_GC
Cuts at: 0 1718 3420
Size: 1718 1702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2962 2996 3420
Size: 2962 34 424
Fragments arranged by size:
2962 424 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 637 895 3420
Size: 637 258 2525
Fragments arranged by size:
2525 637 258
BclI T'GATC_A
Cuts at: 0 156 423 2484 3420
Size: 156 267 2061 936
Fragments arranged by size:
2061 936 267 156
BglI GCCn_nnn'nGGC
Cuts at: 0 770 1592 3420
Size: 770 822 1828
Fragments arranged by size:
1828 822 770
BglII A'GATC_T
Cuts at: 0 1232 2056 3420
Size: 1232 824 1364
Fragments arranged by size:
1364 1232 824
BlpI GC'TnA_GC
Cuts at: 0 1616 3420
Size: 1616 1804
Bme1580I G_kGCm'C
Cuts at: 0 556 784 1285 1293 1551 1735 1809 1910
Size: 556 228 501 8 258 184 74 101
Cuts at: 1910 2128 2268 2368 3420
Size: 218 140 100 1052
Fragments arranged by size:
1052 556 501 258 228 218 184 140
101 100 74 8
BmgBI CAC'GTC
Cuts at: 0 615 744 863 2823 3420
Size: 615 129 119 1960 597
Fragments arranged by size:
1960 615 597 129 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 438 1454 1976 2130 2450 2777 2796
Size: 117 321 1016 522 154 320 327 19
Cuts at: 2796 2939 3420
Size: 143 481
Fragments arranged by size:
1016 522 481 327 321 320 154 143
117 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2255 3420
Size: 2255 1165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 770 1166 3420
Size: 390 380 396 2254
Fragments arranged by size:
2254 396 390 380
Bpu10I CC'TnA_GC
Cuts at: 0 25 1718 3067 3368 3420
Size: 25 1693 1349 301 52
Fragments arranged by size:
1693 1349 301 52 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 958 3420
Size: 958 2462
BsaAI yAC'GTr
Cuts at: 0 1002 1544 1598 2144 3420
Size: 1002 542 54 546 1276
Fragments arranged by size:
1276 1002 546 542 54
BsaBI GATnn'nnATC
Cuts at: 0 422 3420
Size: 422 2998
BsaHI Gr'CG_yC
Cuts at: 0 1958 3276 3420
Size: 1958 1318 144
Fragments arranged by size:
1958 1318 144
BsaWI w'CCGG_w
Cuts at: 0 649 3420
Size: 649 2771
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1185 1215 3420
Size: 1185 30 2205
Fragments arranged by size:
2205 1185 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 743 3420
Size: 743 2677
BseYI C'CCAG_C
Cuts at: 0 386 1727 3420
Size: 386 1341 1693
Fragments arranged by size:
1693 1341 386
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 426 1524 2007 2027 2208 2910 3420
Size: 162 264 1098 483 20 181 702 510
Fragments arranged by size:
1098 702 510 483 264 181 162 20
BsiHKAI G_wGCw'C
Cuts at: 0 1285 1551 1809 2268 2978 3420
Size: 1285 266 258 459 710 442
Fragments arranged by size:
1285 710 459 442 266 258
BsiWI C'GTAC_G
Cuts at: 0 1598 3420
Size: 1598 1822
BsmI GAATG_Cn'
Cuts at: 0 631 760 844 3420
Size: 631 129 84 2576
Fragments arranged by size:
2576 631 129 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3283 3420
Size: 3283 137
Bsp1286I G_dGCh'C
Cuts at: 0 474 556 784 1076 1285 1293 1551 1735
Size: 474 82 228 292 209 8 258 184
Cuts at: 1735 1809 1910 2128 2219 2268 2368 2978 3420
Size: 74 101 218 91 49 100 610 442
Fragments arranged by size:
610 474 442 292 258 228 218 209
184 101 100 91 82 74 49 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 613 976 1546 2029 2121 2434 3344 3420
Size: 613 363 570 483 92 313 910 76
Fragments arranged by size:
910 613 570 483 363 313 92 76
BsrBI CCG'CTC
Cuts at: 0 1007 3420
Size: 1007 2413
BsrDI GCAATG_nn'
Cuts at: 0 888 1665 1974 2573 2751 2935 3420
Size: 888 777 309 599 178 184 485
Fragments arranged by size:
888 777 599 485 309 184 178
BsrFI r'CCGG_y
Cuts at: 0 449 1924 2940 3159 3420
Size: 449 1475 1016 219 261
Fragments arranged by size:
1475 1016 449 261 219
BssSI C'ACGA_G
Cuts at: 0 171 3420
Size: 171 3249
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1033 2093 3420
Size: 1033 1060 1327
Fragments arranged by size:
1327 1060 1033
BstXI CCAn_nnnn'nTGG
Cuts at: 0 58 1642 2311 2440 3420
Size: 58 1584 669 129 980
Fragments arranged by size:
1584 980 669 129 58
BstYI r'GATC_y
Cuts at: 0 666 1232 1838 2056 3288 3420
Size: 666 566 606 218 1232 132
Fragments arranged by size:
1232 666 606 566 218 132
Bsu36I CC'TnA_GG
Cuts at: 0 1067 3420
Size: 1067 2353
BtgI C'CryG_G
Cuts at: 0 342 1658 3420
Size: 342 1316 1762
Fragments arranged by size:
1762 1316 342
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 431 1849 3420
Size: 431 1418 1571
Fragments arranged by size:
1571 1418 431
BtsI GCAGTG_nn'
Cuts at: 0 438 2094 2323 3420
Size: 438 1656 229 1097
Fragments arranged by size:
1656 1097 438 229
DraI TTT'AAA
Cuts at: 0 201 3420
Size: 201 3219
DraIII CAC_nnn'GTG
Cuts at: 0 700 788 1437 2804 3420
Size: 700 88 649 1367 616
Fragments arranged by size:
1367 700 649 616 88
EarI CTCTTCn'nnn_
Cuts at: 0 2614 2899 3162 3420
Size: 2614 285 263 258
Fragments arranged by size:
2614 285 263 258
EcoICRI GAG'CTC
Cuts at: 0 2976 3420
Size: 2976 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 770 1166 2354 3188 3420
Size: 390 380 396 1188 834 232
Fragments arranged by size:
1188 834 396 390 380 232
EcoO109I rG'GnC_Cy
Cuts at: 0 433 941 1535 1731 1732 1907 2124 2444
Size: 433 508 594 196 1 175 217 320
Cuts at: 2444 2911 3420
Size: 467 509
Fragments arranged by size:
594 509 508 467 433 320 217 196
175 1
EcoRI G'AATT_C
Cuts at: 0 3036 3420
Size: 3036 384
HaeII r_GCGC'y
Cuts at: 0 531 1047 1623 1961 2177 3420
Size: 531 516 576 338 216 1243
Fragments arranged by size:
1243 576 531 516 338 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 1185 1217 2469 2501 3139 3171
Size: 222 32 931 32 1252 32 638 32
Cuts at: 3171 3343 3375 3420
Size: 172 32 45
Fragments arranged by size:
1252 931 638 222 172 45 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 981 2826 3420
Size: 981 1845 594
Fragments arranged by size:
1845 981 594
HindIII A'AGCT_T
Cuts at: 0 1220 3420
Size: 1220 2200
KasI G'GCGC_C
Cuts at: 0 1957 3420
Size: 1957 1463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2059 2487 3420
Size: 2059 428 933
Fragments arranged by size:
2059 933 428
MslI CAynn'nnrTG
Cuts at: 0 1059 1497 1554 1640 2271 2744 3420
Size: 1059 438 57 86 631 473 676
Fragments arranged by size:
1059 676 631 473 438 86 57
MspA1I CmG'CkG
Cuts at: 0 542 632 1241 1673 1904 3420
Size: 542 90 609 432 231 1516
Fragments arranged by size:
1516 609 542 432 231 90
NaeI GCC'GGC
Cuts at: 0 2942 3161 3420
Size: 2942 219 259
Fragments arranged by size:
2942 259 219
NarI GG'CG_CC
Cuts at: 0 1958 3420
Size: 1958 1462
NcoI C'CATG_G
Cuts at: 0 342 3420
Size: 342 3078
NdeI CA'TA_TG
Cuts at: 0 2379 3087 3180 3294 3420
Size: 2379 708 93 114 126
Fragments arranged by size:
2379 708 126 114 93
NgoMIV G'CCGG_C
Cuts at: 0 2940 3159 3420
Size: 2940 219 261
Fragments arranged by size:
2940 261 219
NspI r_CATG'y
Cuts at: 0 1016 1199 1496 1883 2670 3420
Size: 1016 183 297 387 787 750
Fragments arranged by size:
1016 787 750 387 297 183
PasI CC'CwG_GG
Cuts at: 0 2899 3420
Size: 2899 521
PciI A'CATG_T
Cuts at: 0 1195 3420
Size: 1195 2225
PfoI T'CCnGG_A
Cuts at: 0 285 897 1203 3420
Size: 285 612 306 2217
Fragments arranged by size:
2217 612 306 285
PmlI CAC'GTG
Cuts at: 0 1002 1544 2144 3420
Size: 1002 542 600 1276
Fragments arranged by size:
1276 1002 600 542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2721 2753 3420
Size: 2721 32 667
Fragments arranged by size:
2721 667 32
PpuMI rG'GwC_Cy
Cuts at: 0 433 941 1535 2444 3420
Size: 433 508 594 909 976
Fragments arranged by size:
976 909 594 508 433
PspOMI G'GGCC_C
Cuts at: 0 1731 1906 2124 3420
Size: 1731 175 218 1296
Fragments arranged by size:
1731 1296 218 175
PstI C_TGCA'G
Cuts at: 0 1240 1633 1873 2173 2458 3420
Size: 1240 393 240 300 285 962
Fragments arranged by size:
1240 962 393 300 285 240
PvuII CAG'CTG
Cuts at: 0 1241 3420
Size: 1241 2179
SacI G_AGCT'C
Cuts at: 0 2978 3420
Size: 2978 442
SanDI GG'GwC_CC
Cuts at: 0 433 3420
Size: 433 2987
ScaI AGT'ACT
Cuts at: 0 2538 3420
Size: 2538 882
SfcI C'TryA_G
Cuts at: 0 1236 1629 1869 2169 2454 3236 3420
Size: 1236 393 240 300 285 782 184
Fragments arranged by size:
1236 782 393 300 285 240 184
SfoI GGC'GCC
Cuts at: 0 1959 3420
Size: 1959 1461
SmlI C'TyrA_G
Cuts at: 0 2523 3420
Size: 2523 897
SphI G_CATG'C
Cuts at: 0 1016 2670 3420
Size: 1016 1654 750
Fragments arranged by size:
1654 1016 750
StyI C'CwwG_G
Cuts at: 0 342 3252 3420
Size: 342 2910 168
Fragments arranged by size:
2910 342 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2815 3420
Size: 2815 605
TatI w'GTAC_w
Cuts at: 0 1411 2320 2536 3111 3420
Size: 1411 909 216 575 309
Fragments arranged by size:
1411 909 575 309 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1799 1831 1989 2021 2073 2105 3420
Size: 1799 32 158 32 52 32 1315
Fragments arranged by size:
1799 1315 158 52 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 613 3420
Size: 613 2807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 592 850 2117 3420
Size: 463 129 258 1267 1303
Fragments arranged by size:
1303 1267 463 258 129
XmnI GAAnn'nnTTC
Cuts at: 0 3036 3171 3420
Size: 3036 135 249
Fragments arranged by size:
3036 249 135
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI
HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI ScaI SfcI SfoI SmlI SphI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AscI AseI
AsiSI AvrII BaeI BfrBI BmtI BpuEI BsiEI BspEI
BspHI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI
DrdI EaeI EagI EciI EcoNI EcoRV FalI FseI
FspI FspAI HpaI KpnI MfeI MluI MscI NheI
NotI NruI NsiI PacI PflMI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI XbaI XhoI XmaI ZraI