(Circular) (Six-base) MAPSORT of: pFN21ASDA1754.seq Check: 2444 from: 1 to: 6370 pFN21ASDA1754 6370 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2224 2614 2224 Size: 390 5980 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3713 1937 Size: 1776 4594 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 3341 4433 5817 6085 1632 Size: 1412 297 1092 1384 268 1917 Fragments arranged by size: 1917 1412 1384 1092 297 268 AfeI AGC'GCT Cuts at: 2465 3609 2465 Size: 1144 5226 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6173 AflIII A'CryG_T Cuts at: 1203 2269 3631 5269 6246 1203 Size: 1066 1362 1638 977 1327 Fragments arranged by size: 1638 1362 1327 1066 977 AgeI A'CCGG_T Cuts at: 3290 5201 3290 Size: 1911 4459 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 2362 2641 1166 Size: 1196 279 4895 Fragments arranged by size: 4895 1196 279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6370 AlwNI CAG_nnn'CTG Cuts at: 2955 4113 5685 2955 Size: 1158 1572 3640 Fragments arranged by size: 3640 1572 1158 ApaI G_GGCC'C Cuts at: 2355 2355 Size: 6370 ApaLI G'TGCA_C Cuts at: 4427 5583 4427 Size: 1156 5214 ApoI r'AATT_y Cuts at: 1493 3679 3905 4057 6166 1493 Size: 2186 226 152 2109 1697 Fragments arranged by size: 2186 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2502 2672 2807 3013 3221 3695 Size: 73 171 388 170 135 206 208 474 Cuts at: 3695 1870 Size: 4545 Fragments arranged by size: 4545 474 388 208 206 171 170 135 73 BamHI G'GATC_C Cuts at: 3700 4067 3700 Size: 367 6003 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2405 2851 3137 3227 3472 Size: 325 217 627 618 446 286 90 245 Cuts at: 3472 3691 5146 618 Size: 219 1455 1842 Fragments arranged by size: 1842 1455 627 618 446 325 286 245 219 217 90 BanII G_rGCy'C Cuts at: 1391 2037 2355 2679 2700 3129 3306 3519 3689 Size: 646 318 324 21 429 177 213 170 Cuts at: 3689 1391 Size: 4072 Fragments arranged by size: 4072 646 429 324 318 213 177 170 21 BbeI G_GCGC'C Cuts at: 2409 2855 3231 2409 Size: 446 376 5548 Fragments arranged by size: 5548 446 376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2820 3329 928 Size: 971 921 509 3969 Fragments arranged by size: 3969 971 921 509 BbvCI CC'TCA_GC Cuts at: 3239 3239 Size: 6370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4561 4595 1903 Size: 34 2624 34 3678 Fragments arranged by size: 3678 2624 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4262 5472 4262 Size: 1210 5160 BclI T'GATC_A Cuts at: 1548 1548 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4981 136 Size: 107 122 71 631 605 3309 1525 Fragments arranged by size: 3309 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6365 6365 Size: 6370 BlpI GC'TnA_GC Cuts at: 3305 3787 3305 Size: 482 5888 Bme1580I G_kGCm'C Cuts at: 1790 2355 2661 2724 3140 4431 5587 1790 Size: 565 306 63 416 1291 1156 2573 Fragments arranged by size: 2573 1291 1156 565 416 306 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4101 5059 442 Size: 416 53 83 399 24 2684 958 1753 Fragments arranged by size: 2684 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2371 2371 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2207 2249 2308 3133 3416 3437 5030 Size: 953 15 42 59 825 283 21 1593 Cuts at: 5030 1239 Size: 2579 Fragments arranged by size: 2579 1593 953 825 283 59 42 21 15 Bpu10I CC'TnA_GC Cuts at: 3239 3506 3239 Size: 267 6103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3578 4495 5360 5658 3578 Size: 917 865 298 4290 Fragments arranged by size: 4290 917 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5033 882 Size: 617 3534 2219 Fragments arranged by size: 3534 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3667 4161 493 Size: 711 2463 494 2702 Fragments arranged by size: 2702 2463 711 494 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2406 2852 3228 4177 Size: 53 83 186 936 873 446 376 949 Cuts at: 4177 4559 5194 275 Size: 382 635 1451 Fragments arranged by size: 1451 949 936 873 635 446 382 376 186 83 53 BsaWI w'CCGG_w Cuts at: 3290 4803 5201 5475 5622 6033 3290 Size: 1513 398 274 147 411 3627 Fragments arranged by size: 3627 1513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3092 3122 3324 3354 1178 Size: 30 165 30 1689 30 202 30 4194 Fragments arranged by size: 4194 1689 202 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2141 2204 2207 2258 2393 2563 3338 Size: 952 3 63 3 51 135 170 775 Cuts at: 3338 1186 Size: 4218 Fragments arranged by size: 4218 952 775 170 135 63 51 3 3 BseYI C'CCAG_C Cuts at: 2398 3142 3296 3604 5573 6069 2398 Size: 744 154 308 1969 496 2699 Fragments arranged by size: 2699 1969 744 496 308 154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2816 3320 3380 1163 Size: 573 1080 504 60 4153 Fragments arranged by size: 4153 1080 573 504 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2214 2487 2713 3820 4581 5609 Size: 275 186 213 273 226 1107 761 1028 Cuts at: 5609 1540 Size: 2301 Fragments arranged by size: 2301 1107 1028 761 275 273 226 213 186 BsiHKAI G_wGCw'C Cuts at: 2294 2700 2708 3507 4431 4516 5587 2294 Size: 406 8 799 924 85 1071 3077 Fragments arranged by size: 3077 1071 924 799 406 85 8 BsmI GAATG_Cn' Cuts at: 3604 3881 3974 3604 Size: 277 93 6000 Fragments arranged by size: 6000 277 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2294 2355 2661 2679 2700 2708 Size: 399 247 257 61 306 18 21 8 Cuts at: 2708 2724 3129 3140 3306 3507 3519 3689 4431 Size: 16 405 11 166 201 12 170 742 Cuts at: 4431 4516 5587 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 306 257 247 201 170 166 85 61 21 18 16 12 11 8 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 2922 4259 5989 2922 Size: 1337 1730 3303 Fragments arranged by size: 3303 1730 1337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 2614 2886 2908 3725 844 Size: 1380 390 272 22 817 3489 Fragments arranged by size: 3489 1380 817 390 272 22 BsrBI CCG'CTC Cuts at: 1394 1823 2715 4257 1394 Size: 429 892 1542 3507 Fragments arranged by size: 3507 1542 892 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4865 5039 65 Size: 1183 3617 174 1396 Fragments arranged by size: 3617 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3290 5014 5201 1700 Size: 110 1480 1724 187 2869 Fragments arranged by size: 2869 1724 1480 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 3398 4430 5442 3398 Size: 1032 1012 4326 Fragments arranged by size: 4326 1032 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2298 3403 794 Size: 1504 1105 3761 Fragments arranged by size: 3761 1504 1105 BstBI TT'CG_AA Cuts at: 2007 5174 2007 Size: 3167 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6370 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2700 2029 Size: 671 5699 BstYI r'GATC_y Cuts at: 1972 3700 4067 4452 4469 5921 6007 6019 6365 Size: 1728 367 385 17 1452 86 12 346 Cuts at: 6365 1972 Size: 1977 Fragments arranged by size: 1977 1728 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2546 3197 3477 3495 2546 Size: 651 280 18 5421 Fragments arranged by size: 5421 651 280 18 BtgI C'CryG_G Cuts at: 513 1065 2022 2028 2093 2481 3483 513 Size: 552 957 6 65 388 1002 3400 Fragments arranged by size: 3400 1002 957 552 388 65 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2950 487 Size: 683 56 1724 3907 Fragments arranged by size: 3907 1724 683 56 BtsI GCAGTG_nn' Cuts at: 2636 3215 3886 4680 4700 2636 Size: 579 671 794 20 4306 Fragments arranged by size: 4306 794 671 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5377 809 Size: 507 4061 1802 Fragments arranged by size: 4061 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2064 2211 Size: 54 1425 50 275 18 195 39 147 Cuts at: 2211 2449 2484 2710 2743 2998 3817 4706 6335 Size: 238 35 226 33 255 819 889 1629 Cuts at: 6335 8 Size: 43 Fragments arranged by size: 1629 1425 889 819 275 255 238 226 195 147 54 50 43 39 35 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2211 2484 2710 3817 1537 Size: 275 399 273 226 1107 4090 Fragments arranged by size: 4090 1107 399 275 273 226 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2315 3416 3533 4300 1353 Size: 689 273 1101 117 767 3423 Fragments arranged by size: 3423 1101 767 689 273 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2255 4957 5331 5477 387 Size: 1064 138 666 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 666 374 146 138 EcoICRI GAG'CTC Cuts at: 2698 2698 Size: 6370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2207 2249 2308 3044 3133 3341 Size: 393 560 15 42 59 736 89 208 Cuts at: 3341 3416 3437 4433 5030 5817 6085 1239 Size: 75 21 996 597 787 268 1524 Fragments arranged by size: 1524 996 787 736 597 560 393 268 208 89 75 59 42 21 15 EcoNI CCTnn'n_nnAGG Cuts at: 2189 3353 2189 Size: 1164 5206 EcoO109I rG'GnC_Cy Cuts at: 2281 2351 2352 2419 3018 2281 Size: 70 1 67 599 5633 Fragments arranged by size: 5633 599 70 67 1 EcoRI G'AATT_C Cuts at: 3679 4057 3679 Size: 378 5992 FspI TGC'GCA Cuts at: 4876 4876 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 2409 2467 2855 3231 3611 5517 1678 Size: 731 58 388 376 380 1906 2531 Fragments arranged by size: 2531 1906 731 388 380 376 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3180 3212 5011 5043 5085 Size: 32 135 32 1608 32 1799 32 42 Cuts at: 5085 5117 1373 Size: 32 2626 Fragments arranged by size: 2626 1799 1608 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3714 3960 669 Size: 988 281 1776 246 3079 Fragments arranged by size: 3079 1776 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6370 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KasI G'GCGC_C Cuts at: 2405 2851 3227 2405 Size: 446 376 5548 Fragments arranged by size: 5548 446 376 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 2269 6246 2269 Size: 3977 2393 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5484 5668 1305 Size: 4179 184 2007 Fragments arranged by size: 4179 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2451 3000 10 Size: 54 1425 343 195 424 549 3380 Fragments arranged by size: 3380 1425 549 424 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2362 2457 2573 2579 2597 2641 Size: 648 861 335 95 116 6 18 44 Cuts at: 2641 4328 4687 4846 518 Size: 1687 359 159 2042 Fragments arranged by size: 2042 1687 861 648 359 335 159 116 95 44 18 6 MspA1I CmG'CkG Cuts at: 1606 2083 2095 2170 3004 3243 3300 3382 3786 Size: 477 12 75 834 239 57 82 404 Cuts at: 3786 4463 5611 5856 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 834 677 477 404 245 239 82 75 57 12 NarI GG'CG_CC Cuts at: 2406 2852 3228 2406 Size: 446 376 5548 Fragments arranged by size: 5548 446 376 NcoI C'CATG_G Cuts at: 513 1065 2022 2028 3483 513 Size: 552 957 6 1455 3400 Fragments arranged by size: 3400 1455 957 552 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 2611 2821 3635 3728 5273 1132 Size: 97 1382 210 814 93 1545 2229 Fragments arranged by size: 2229 1545 1382 814 210 97 93 PasI CC'CwG_GG Cuts at: 1348 3120 1348 Size: 1772 4598 PciI A'CATG_T Cuts at: 3631 5269 3631 Size: 1638 4732 PflMI CCAn_nnn'nTGG Cuts at: 1108 2955 1108 Size: 1847 4523 PfoI T'CCnGG_A Cuts at: 2511 3414 2511 Size: 903 5467 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 3018 2281 Size: 737 5633 PshAI GACnn'nnGTC Cuts at: 1613 3390 1613 Size: 1777 4593 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PspOMI G'GGCC_C Cuts at: 2351 2351 Size: 6370 PstI C_TGCA'G Cuts at: 830 2169 3252 3549 3722 830 Size: 1339 1083 297 173 3478 Fragments arranged by size: 3478 1339 1083 297 173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 2170 3004 3243 3382 2170 Size: 834 239 139 5158 Fragments arranged by size: 5158 834 239 139 SacI G_AGCT'C Cuts at: 2700 2700 Size: 6370 SacII CC_GC'GG Cuts at: 2096 2096 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SanDI GG'GwC_CC Cuts at: 3018 3018 Size: 6370 SapI GCTCTTCn'nnn_ Cuts at: 2042 3416 2042 Size: 1374 4996 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 2165 3248 3545 3718 4853 5534 5725 Size: 219 1120 1083 297 173 1135 681 191 Cuts at: 5725 826 Size: 1471 Fragments arranged by size: 1471 1135 1120 1083 681 297 219 191 173 SfoI GGC'GCC Cuts at: 2407 2853 3229 2407 Size: 446 376 5548 Fragments arranged by size: 5548 446 376 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 2504 3015 3697 1872 Size: 632 511 682 4545 Fragments arranged by size: 4545 682 632 511 SmlI C'TyrA_G Cuts at: 820 1017 1943 3593 4474 5375 5637 820 Size: 197 926 1650 881 901 262 1553 Fragments arranged by size: 1650 1553 926 901 881 262 197 SnaBI TAC'GTA Cuts at: 493 3667 4161 493 Size: 3174 494 2702 Fragments arranged by size: 3174 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3728 1132 Size: 2596 3774 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6370 StyI C'CwwG_G Cuts at: 513 1065 2022 2028 3483 3809 513 Size: 552 957 6 1455 326 3074 Fragments arranged by size: 3074 1455 957 552 326 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2706 4397 4414 4567 4752 5197 325 Size: 1255 1126 1691 17 153 185 445 1498 Fragments arranged by size: 1691 1498 1255 1126 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3099 4616 6328 Size: 274 80 33 51 1443 1122 1517 1712 Cuts at: 6328 96 Size: 138 Fragments arranged by size: 1712 1517 1443 1122 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6330 6362 6330 Size: 32 6338 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6370 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 2502 3013 3695 1870 Size: 632 511 682 4545 Fragments arranged by size: 4545 682 632 511 XmnI GAAnn'nnTTC Cuts at: 2930 4499 2930 Size: 1569 4801 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI