(Circular) (Six-base) MAPSORT of: pFN21ASDA1753.seq Check: 9873 from: 1 to: 7159 pFN21ASDA1753 7159 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2210 4138 2210 Size: 1928 5231 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2286 4969 278 Size: 53 83 186 936 750 2683 2468 Fragments arranged by size: 2683 2468 936 750 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 2502 4502 1937 Size: 349 216 2000 4594 Fragments arranged by size: 4594 2000 349 216 Acc65I G'GTAC_C Cuts at: 2379 4480 2379 Size: 2101 5058 AclI AA'CG_TT Cuts at: 1563 5286 5659 1563 Size: 3723 373 3063 Fragments arranged by size: 3723 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 3365 5222 6606 6874 1632 Size: 872 861 1857 1384 268 1917 Fragments arranged by size: 1917 1857 1384 872 861 268 AflII C'TTAA_G Cuts at: 820 1017 4331 820 Size: 197 3314 3648 Fragments arranged by size: 3648 3314 197 AflIII A'CryG_T Cuts at: 1203 2124 6058 7035 1203 Size: 921 3934 977 1327 Fragments arranged by size: 3934 1327 977 921 AgeI A'CCGG_T Cuts at: 5990 5990 Size: 7159 AhdI GACnn_n'nnGTC Cuts at: 5888 5888 Size: 7159 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2664 2942 3698 3708 4149 1184 Size: 1480 278 756 10 441 4194 Fragments arranged by size: 4194 1480 756 441 278 10 AlwNI CAG_nnn'CTG Cuts at: 2092 3376 3885 3953 4207 4902 6474 2092 Size: 1284 509 68 254 695 1572 2777 Fragments arranged by size: 2777 1572 1284 695 509 254 68 ApaI G_GGCC'C Cuts at: 2036 2043 2481 4279 4438 2036 Size: 7 438 1798 159 4757 Fragments arranged by size: 4757 1798 438 159 7 ApaLI G'TGCA_C Cuts at: 5216 6372 5216 Size: 1156 6003 ApoI r'AATT_y Cuts at: 1493 2138 3313 4468 4694 4846 6955 1493 Size: 645 1175 1155 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 1155 645 226 152 AseI AT'TA_AT Cuts at: 160 1032 5713 160 Size: 872 4681 1606 Fragments arranged by size: 4681 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2105 2894 2949 3916 4119 4484 Size: 73 93 69 789 55 967 203 365 Cuts at: 4484 1870 Size: 4545 Fragments arranged by size: 4545 967 789 365 203 93 73 69 55 AvrII C'CTAG_G Cuts at: 3148 3148 Size: 7159 BamHI G'GATC_C Cuts at: 4489 4856 4489 Size: 367 6792 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2232 2261 2379 3082 Size: 325 217 627 278 167 29 118 703 Cuts at: 3082 3491 4100 4480 5935 618 Size: 409 609 380 1455 1842 Fragments arranged by size: 1842 1455 703 627 609 409 380 325 278 217 167 118 29 BanII G_rGCy'C Cuts at: 1391 2036 2043 2481 2762 3095 3188 3357 3692 Size: 645 7 438 281 333 93 169 335 Cuts at: 3692 4200 4279 4392 4438 4478 1391 Size: 508 79 113 46 40 4072 Fragments arranged by size: 4072 645 508 438 335 333 281 169 113 93 79 46 40 7 BbeI G_GCGC'C Cuts at: 2069 2236 4104 2069 Size: 167 1868 5124 Fragments arranged by size: 5124 1868 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3214 3291 3629 928 Size: 971 1315 77 338 4458 Fragments arranged by size: 4458 1315 971 338 77 BbvCI CC'TCA_GC Cuts at: 2621 3016 3838 4398 2621 Size: 395 822 560 5382 Fragments arranged by size: 5382 822 560 395 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2043 2077 3649 3683 5350 5384 1903 Size: 34 106 34 1572 34 1667 34 3678 Fragments arranged by size: 3678 1667 1572 106 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5051 6261 5051 Size: 1210 5949 BclI T'GATC_A Cuts at: 1548 1548 Size: 7159 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2328 2531 2638 Size: 107 122 71 631 605 656 203 107 Cuts at: 2638 2698 2712 3489 3962 4040 4094 4428 5770 Size: 60 14 777 473 78 54 334 1342 Cuts at: 5770 136 Size: 1525 Fragments arranged by size: 1525 1342 777 656 631 605 473 334 203 122 107 107 78 71 60 54 14 BglII A'GATC_T Cuts at: 2898 7154 2898 Size: 4256 2903 BlpI GC'TnA_GC Cuts at: 4576 4576 Size: 7159 Bme1580I G_kGCm'C Cuts at: 1790 2036 2043 2159 2342 2481 3127 3494 3648 Size: 246 7 116 183 139 646 367 154 Cuts at: 3648 3923 4279 4415 4438 5220 6376 1790 Size: 275 356 136 23 782 1156 2573 Fragments arranged by size: 2573 1156 782 646 367 356 275 246 183 154 139 136 116 23 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3479 4890 5848 Size: 416 53 83 399 24 2062 1411 958 Cuts at: 5848 442 Size: 1753 Fragments arranged by size: 2062 1753 1411 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7159 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2443 4252 2443 Size: 1809 5350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3082 3230 3442 3638 4145 4160 4193 4244 Size: 1843 148 212 196 507 15 33 51 Cuts at: 4244 4247 4430 5819 1239 Size: 3 183 1389 2579 Fragments arranged by size: 2579 1843 1389 507 212 196 183 148 51 33 15 3 Bpu10I CC'TnA_GC Cuts at: 2085 2621 3016 3838 4194 4398 2085 Size: 536 395 822 356 204 4846 Fragments arranged by size: 4846 822 536 395 356 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3418 5284 6149 6447 3418 Size: 1866 865 298 4130 Fragments arranged by size: 4130 1866 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2817 5822 882 Size: 617 1318 3005 2219 Fragments arranged by size: 3005 2219 1318 617 BsaAI yAC'GTr Cuts at: 493 1204 2127 2977 4456 4950 493 Size: 711 923 850 1479 494 2702 Fragments arranged by size: 2702 1479 923 850 711 494 BsaBI GATnn'nnATC Cuts at: 7153 7153 Size: 7159 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2233 2283 2850 Size: 53 83 186 936 533 167 50 567 Cuts at: 2850 4101 4966 5348 5983 275 Size: 1251 865 382 635 1451 Fragments arranged by size: 1451 1251 936 865 635 567 533 382 186 167 83 53 50 BsaWI w'CCGG_w Cuts at: 5592 5990 6264 6411 6822 5592 Size: 398 274 147 411 5929 Fragments arranged by size: 5929 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2477 2507 2621 2651 3066 Size: 30 165 30 1074 30 114 30 415 Cuts at: 3066 3096 1178 Size: 30 5241 Fragments arranged by size: 5241 1074 415 165 114 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2642 3119 3763 4031 4085 4181 1186 Size: 1456 477 644 268 54 96 4164 Fragments arranged by size: 4164 1456 644 477 268 96 54 BseYI C'CCAG_C Cuts at: 2585 2659 3036 3400 6362 6858 2585 Size: 74 377 364 2962 496 2886 Fragments arranged by size: 2962 2886 496 377 364 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2275 2333 2425 2441 4166 1163 Size: 573 539 58 92 16 1725 4156 Fragments arranged by size: 4156 1725 573 539 92 58 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2076 4609 5370 6398 1540 Size: 275 186 75 2533 761 1028 2301 Fragments arranged by size: 2533 2301 1028 761 275 186 75 BsiHKAI G_wGCw'C Cuts at: 3692 4392 5220 5305 6376 3692 Size: 700 828 85 1071 4475 Fragments arranged by size: 4475 1071 828 700 85 BsiWI C'GTAC_G Cuts at: 4452 4452 Size: 7159 BsmI GAATG_Cn' Cuts at: 3520 4670 4763 3520 Size: 1150 93 5916 Fragments arranged by size: 5916 1150 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2790 2841 2790 Size: 51 7108 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2043 2159 2342 2481 2762 3095 Size: 399 246 7 116 183 139 281 333 Cuts at: 3095 3127 3188 3357 3494 3648 3692 3923 4200 Size: 32 61 169 137 154 44 231 277 Cuts at: 4200 4279 4392 4415 4438 4478 5220 5305 6376 Size: 79 113 23 23 40 742 85 1071 Cuts at: 6376 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 333 281 277 246 231 183 169 154 139 137 116 113 85 79 61 44 40 32 23 23 7 BspEI T'CCGG_A Cuts at: 6822 6822 Size: 7159 BspHI T'CATG_A Cuts at: 5048 6778 5048 Size: 1730 5429 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2210 2367 3012 4138 4514 844 Size: 1366 157 645 1126 376 3489 Fragments arranged by size: 3489 1366 1126 645 376 157 BsrBI CCG'CTC Cuts at: 1394 1823 2084 2493 4084 4177 5046 1394 Size: 429 261 409 1591 93 869 3507 Fragments arranged by size: 3507 1591 869 429 409 261 93 BsrDI GCAATG_nn' Cuts at: 65 1248 5654 5828 65 Size: 1183 4406 174 1396 Fragments arranged by size: 4406 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2238 2748 5803 5990 1700 Size: 110 428 510 3055 187 2869 Fragments arranged by size: 3055 2869 510 428 187 110 BsrGI T'GTAC_A Cuts at: 96 3054 96 Size: 2958 4201 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7159 BssSI C'ACGA_G Cuts at: 2417 5219 6231 2417 Size: 2802 1012 3345 Fragments arranged by size: 3345 2802 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3376 3784 4410 794 Size: 2582 408 626 3543 Fragments arranged by size: 3543 2582 626 408 BstBI TT'CG_AA Cuts at: 2007 5963 2007 Size: 3956 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7159 BstXI CCAn_nnnn'nTGG Cuts at: 2585 2585 Size: 7159 BstYI r'GATC_y Cuts at: 1972 2616 2898 3851 4489 4856 5241 5258 6710 Size: 644 282 953 638 367 385 17 1452 Cuts at: 6710 6796 6808 7154 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 953 644 638 385 367 346 282 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2473 2526 2553 2696 513 Size: 552 1408 53 27 143 4976 Fragments arranged by size: 4976 1408 552 143 53 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6420 Fragments arranged by size: 6420 683 56 BtsI GCAGTG_nn' Cuts at: 2159 2458 4016 4362 4675 5469 5489 2159 Size: 299 1558 346 313 794 20 3829 Fragments arranged by size: 3829 1558 794 346 313 299 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7159 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7124 DraI TTT'AAA Cuts at: 4463 4463 Size: 7159 DraIII CAC_nnn'GTG Cuts at: 2221 2916 2221 Size: 695 6464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6166 809 Size: 507 4850 1802 Fragments arranged by size: 4850 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2545 3609 Size: 54 1425 50 275 18 243 472 1064 Cuts at: 3609 4429 4606 5495 7124 8 Size: 820 177 889 1629 43 Fragments arranged by size: 1629 1425 1064 889 820 472 275 243 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2073 4606 1537 Size: 275 261 2533 4090 Fragments arranged by size: 4090 2533 275 261 EarI CTCTTCn'nnn_ Cuts at: 1353 3448 5089 1353 Size: 2095 1641 3423 Fragments arranged by size: 3423 2095 1641 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2237 2770 4082 5746 6120 6266 Size: 1064 138 648 533 1312 1664 374 146 Cuts at: 6266 387 Size: 1280 Fragments arranged by size: 1664 1312 1280 1064 648 533 374 146 138 EcoICRI GAG'CTC Cuts at: 3690 4390 3690 Size: 700 6459 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 3082 3230 3365 3442 3638 4145 Size: 393 872 578 148 135 77 196 507 Cuts at: 4145 4160 4193 4244 4247 4430 5222 5819 6606 Size: 15 33 51 3 183 792 597 787 Cuts at: 6606 6874 1239 Size: 268 1524 Fragments arranged by size: 1524 872 792 787 597 578 507 393 268 196 183 148 135 77 51 33 15 3 EcoNI CCTnn'n_nnAGG Cuts at: 2624 2624 Size: 7159 EcoO109I rG'GnC_Cy Cuts at: 2032 2033 2478 2954 3044 4190 4204 2032 Size: 1 445 476 90 1146 14 4987 Fragments arranged by size: 4987 1146 476 445 90 14 1 EcoRI G'AATT_C Cuts at: 2138 4468 4846 2138 Size: 2330 378 4451 Fragments arranged by size: 4451 2330 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3459 3459 Size: 7159 FspI TGC'GCA Cuts at: 5665 5665 Size: 7159 HaeII r_GCGC'y Cuts at: 1678 2069 2236 4104 4235 6306 1678 Size: 391 167 1868 131 2071 2531 Fragments arranged by size: 2531 2071 1868 391 167 131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 2564 2596 5800 Size: 32 135 32 905 32 55 32 3204 Cuts at: 5800 5832 5874 5906 1373 Size: 32 42 32 2626 Fragments arranged by size: 3204 2626 905 135 55 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4503 4749 669 Size: 988 281 2565 246 3079 Fragments arranged by size: 3079 2565 988 281 246 HindIII A'AGCT_T Cuts at: 748 3907 748 Size: 3159 4000 HpaI GTT'AAC Cuts at: 4749 4749 Size: 7159 KasI G'GCGC_C Cuts at: 2065 2232 4100 2065 Size: 167 1868 5124 Fragments arranged by size: 5124 1868 167 KpnI G_GTAC'C Cuts at: 2383 4484 2383 Size: 2101 5058 MfeI C'AATT_G Cuts at: 4758 4758 Size: 7159 MluI A'CGCG_T Cuts at: 7035 7035 Size: 7159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3795 4245 6273 6457 1305 Size: 2490 450 2028 184 2007 Fragments arranged by size: 2490 2028 2007 450 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2547 10 Size: 54 1425 343 715 4622 Fragments arranged by size: 4622 1425 715 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2811 5117 5476 5635 518 Size: 648 1645 2306 359 159 2042 Fragments arranged by size: 2306 2042 1645 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2230 2698 2943 3020 4129 4575 5252 6400 Size: 624 468 245 77 1109 446 677 1148 Cuts at: 6400 6645 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1109 677 624 468 446 245 245 77 NarI GG'CG_CC Cuts at: 2066 2233 4101 2066 Size: 167 1868 5124 Fragments arranged by size: 5124 1868 167 NcoI C'CATG_G Cuts at: 513 1065 2526 513 Size: 552 1461 5146 Fragments arranged by size: 5146 1461 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7159 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7159 NotI GC'GGCC_GC Cuts at: 4606 4606 Size: 7159 NspI r_CATG'y Cuts at: 1132 1229 2929 3061 3110 3400 4315 4517 6062 Size: 97 1700 132 49 290 915 202 1545 Cuts at: 6062 1132 Size: 2229 Fragments arranged by size: 2229 1700 1545 915 290 202 132 97 49 PasI CC'CwG_GG Cuts at: 1348 3227 3796 1348 Size: 1879 569 4711 Fragments arranged by size: 4711 1879 569 PciI A'CATG_T Cuts at: 6058 6058 Size: 7159 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7159 PfoI T'CCnGG_A Cuts at: 4022 4161 4022 Size: 139 7020 PmeI GTTT'AAAC Cuts at: 4463 4463 Size: 7159 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2870 2902 5228 5260 6741 6773 2870 Size: 32 2326 32 1481 32 3256 Fragments arranged by size: 3256 2326 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2954 3044 2954 Size: 90 7069 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7159 PsiI TTA'TAA Cuts at: 4729 4729 Size: 7159 PspOMI G'GGCC_C Cuts at: 2032 2039 2477 4275 4434 2032 Size: 7 438 1798 159 4757 Fragments arranged by size: 4757 1798 438 159 7 PstI C_TGCA'G Cuts at: 830 2097 3009 3687 3789 4511 830 Size: 1267 912 678 102 722 3478 Fragments arranged by size: 3478 1267 912 722 678 102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7159 PvuII CAG'CTG Cuts at: 2943 3020 2943 Size: 77 7082 SacI G_AGCT'C Cuts at: 3692 4392 3692 Size: 700 6459 SacII CC_GC'GG Cuts at: 2699 2699 Size: 7159 SalI G'TCGA_C Cuts at: 1936 4501 1936 Size: 2565 4594 SbfI CC_TGCA'GG Cuts at: 4511 4511 Size: 7159 ScaI AGT'ACT Cuts at: 2861 5407 7119 2861 Size: 2546 1712 2901 Fragments arranged by size: 2901 2546 1712 SexAI A'CCwGG_T Cuts at: 3288 3288 Size: 7159 SfcI C'TryA_G Cuts at: 826 1045 2093 2310 3005 3133 3683 3785 4507 Size: 219 1048 217 695 128 550 102 722 Cuts at: 4507 5642 6323 6514 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 722 695 681 550 219 217 191 128 102 SfiI GGCCn_nnn'nGGCC Cuts at: 2531 2531 Size: 7159 SfoI GGC'GCC Cuts at: 2067 2234 4102 2067 Size: 167 1868 5124 Fragments arranged by size: 5124 1868 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7159 SmaI CCC'GGG Cuts at: 1872 2038 3918 4121 4486 1872 Size: 166 1880 203 365 4545 Fragments arranged by size: 4545 1880 365 203 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 3433 4331 5263 6164 6426 820 Size: 197 926 1490 898 932 901 262 1553 Fragments arranged by size: 1553 1490 932 926 901 898 262 197 SnaBI TAC'GTA Cuts at: 493 4456 4950 493 Size: 3963 494 2702 Fragments arranged by size: 3963 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7159 SphI G_CATG'C Cuts at: 1132 3110 4517 1132 Size: 1978 1407 3774 Fragments arranged by size: 3774 1978 1407 SspI AAT'ATT Cuts at: 5 52 5083 5 Size: 47 5031 2081 Fragments arranged by size: 5031 2081 47 StuI AGG'CCT Cuts at: 1517 2316 2534 1517 Size: 799 218 6142 Fragments arranged by size: 6142 799 218 StyI C'CwwG_G Cuts at: 513 1065 2526 2710 3148 3717 4598 513 Size: 552 1461 184 438 569 881 3074 Fragments arranged by size: 3074 1461 881 569 552 438 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3050 3666 5186 5203 5356 5541 5986 Size: 1255 1470 616 1520 17 153 185 445 Cuts at: 5986 325 Size: 1498 Fragments arranged by size: 1520 1498 1470 1255 616 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 3054 5405 Size: 274 80 33 51 1443 882 195 2351 Cuts at: 5405 7117 96 Size: 1712 138 Fragments arranged by size: 2351 1712 1443 882 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2621 2653 3066 3098 3731 3763 7119 7151 2621 Size: 32 413 32 633 32 3356 32 2629 Fragments arranged by size: 3356 2629 633 413 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7159 XbaI T'CTAG_A Cuts at: 4495 4495 Size: 7159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7159 XmaI C'CCGG_G Cuts at: 1870 2036 3916 4119 4484 1870 Size: 166 1880 203 365 4545 Fragments arranged by size: 4545 1880 365 203 166 XmnI GAAnn'nnTTC Cuts at: 2138 3314 3626 5288 2138 Size: 1176 312 1662 4009 Fragments arranged by size: 4009 1662 1176 312 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2284 4967 276 Size: 53 83 186 936 750 2683 2468 Fragments arranged by size: 2683 2468 936 750 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SrfI SwaI XcmI