(Circular) (Six-base) MAPSORT of: pFN21ASDA1750.seq Check: 8476 from: 1 to: 5980 pFN21ASDA1750 5980 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 4043 5427 5695 1632 Size: 1040 1371 1384 268 1917 Fragments arranged by size: 1917 1384 1371 1040 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 2381 3723 5295 2381 Size: 1342 1572 3066 Fragments arranged by size: 3066 1572 1342 ApaI G_GGCC'C Cuts at: 2296 2607 2296 Size: 311 5669 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 3289 3515 3667 5776 1493 Size: 1796 226 152 2109 1697 Fragments arranged by size: 2109 1796 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2091 2153 2240 2295 2338 2767 3305 Size: 73 148 62 87 55 43 429 538 Cuts at: 3305 1870 Size: 4545 Fragments arranged by size: 4545 538 429 148 87 73 62 55 43 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3301 4756 618 Size: 325 217 627 1514 1455 1842 Fragments arranged by size: 1842 1514 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2296 2607 2877 3184 3299 1391 Size: 905 311 270 307 115 4072 Fragments arranged by size: 4072 905 311 307 270 115 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2166 2377 928 Size: 971 267 211 4531 Fragments arranged by size: 4531 971 267 211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2340 2374 4171 4205 1903 Size: 34 403 34 1797 34 3678 Fragments arranged by size: 3678 1797 403 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2623 3872 5082 2623 Size: 1249 1210 3521 Fragments arranged by size: 3521 1249 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4591 136 Size: 107 122 71 631 605 2919 1525 Fragments arranged by size: 2919 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 2296 2607 4041 5197 1790 Size: 506 311 1434 1156 2573 Fragments arranged by size: 2573 1434 1156 506 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2810 1056 Size: 1754 4226 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2517 2569 2517 Size: 52 5928 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2091 2657 2678 2686 3014 4640 1239 Size: 852 566 21 8 328 1626 2579 Fragments arranged by size: 2579 1626 852 566 328 21 8 Bpu10I CC'TnA_GC Cuts at: 3038 3038 Size: 5980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4105 4970 5268 4105 Size: 865 298 4817 Fragments arranged by size: 4817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3277 3771 493 Size: 711 2073 494 2702 Fragments arranged by size: 2702 2073 711 494 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 3787 4169 4804 Size: 53 83 186 936 582 1672 382 635 Cuts at: 4804 275 Size: 1451 Fragments arranged by size: 1672 1451 936 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3080 3110 1178 Size: 30 165 30 1677 30 4048 Fragments arranged by size: 4048 1677 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2261 1186 Size: 1036 39 4905 Fragments arranged by size: 4905 1036 39 BseYI C'CCAG_C Cuts at: 2564 2608 2802 5183 5679 2564 Size: 44 194 2381 496 2865 Fragments arranged by size: 2865 2381 496 194 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 2666 1163 Size: 573 465 465 4477 Fragments arranged by size: 4477 573 465 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 2920 3430 4191 5219 1540 Size: 275 186 31 888 510 761 1028 2301 Fragments arranged by size: 2301 1028 888 761 510 275 186 31 BsiHKAI G_wGCw'C Cuts at: 2877 2949 4041 4126 5197 2877 Size: 72 1092 85 1071 3660 Fragments arranged by size: 3660 1092 1071 85 72 BsmI GAATG_Cn' Cuts at: 3491 3584 3491 Size: 93 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2296 2607 2877 2949 3184 3299 4041 Size: 399 506 311 270 72 235 115 742 Cuts at: 4041 4126 5197 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 506 399 311 270 235 115 85 72 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3335 844 Size: 2491 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2028 3867 1394 Size: 429 205 1839 3507 Fragments arranged by size: 3507 1839 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2259 2442 4624 4811 1700 Size: 110 449 183 2182 187 2869 Fragments arranged by size: 2869 2182 449 187 183 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 3183 4040 5052 3183 Size: 857 1012 4111 Fragments arranged by size: 4111 1012 857 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 2007 4784 2007 Size: 2777 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 3009 3009 Size: 5980 BstYI r'GATC_y Cuts at: 1972 3310 3677 4062 4079 5531 5617 5629 5975 Size: 1338 367 385 17 1452 86 12 346 Cuts at: 5975 1972 Size: 1977 Fragments arranged by size: 1977 1452 1338 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2502 2502 Size: 5980 BtgI C'CryG_G Cuts at: 513 1065 2311 2322 2406 2487 513 Size: 552 1246 11 84 81 4006 Fragments arranged by size: 4006 1246 552 84 81 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 3496 4290 4310 3496 Size: 794 20 5166 Fragments arranged by size: 5166 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2325 2370 Size: 54 1425 50 275 18 199 296 45 Cuts at: 2370 2977 3427 4316 5945 8 Size: 607 450 889 1629 43 Fragments arranged by size: 1629 1425 889 607 450 296 275 199 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2029 3427 1537 Size: 275 217 1398 4090 Fragments arranged by size: 4090 1398 275 217 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 2865 3910 1353 Size: 1224 288 1045 3423 Fragments arranged by size: 3423 1224 1045 288 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2693 4567 4941 5087 387 Size: 1064 138 498 606 1874 374 146 1280 Fragments arranged by size: 1874 1280 1064 606 498 374 146 138 EcoICRI GAG'CTC Cuts at: 2875 2875 Size: 5980 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2657 2672 2678 2686 3014 4043 Size: 393 459 566 15 6 8 328 1029 Cuts at: 4043 4640 5427 5695 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1029 787 597 566 459 393 328 268 15 8 6 EcoNI CCTnn'n_nnAGG Cuts at: 3041 3041 Size: 5980 EcoO109I rG'GnC_Cy Cuts at: 2467 2498 2603 2604 2685 2467 Size: 31 105 1 81 5762 Fragments arranged by size: 5762 105 81 31 1 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2929 2929 Size: 5980 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2368 5127 1678 Size: 690 2759 2531 Fragments arranged by size: 2759 2531 690 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2582 2614 3017 3049 4621 Size: 32 135 32 1010 32 403 32 1572 Cuts at: 4621 4653 4695 4727 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1572 1010 403 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2416 3294 5094 5278 1305 Size: 1111 878 1800 184 2007 Fragments arranged by size: 2007 1800 1111 878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2979 10 Size: 54 1425 343 1147 3011 Fragments arranged by size: 3011 1425 1147 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3938 4297 4456 518 Size: 648 2772 359 159 2042 Fragments arranged by size: 2772 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2216 2324 2399 2489 2564 2650 3396 4073 Size: 610 108 75 90 75 86 746 677 Cuts at: 4073 5221 5466 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 746 677 610 245 108 90 86 75 75 NaeI GCC'GGC Cuts at: 2261 2261 Size: 5980 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5428 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NgoMIV G'CCGG_C Cuts at: 2259 2259 Size: 5980 NheI G'CTAG_C Cuts at: 1052 2806 1052 Size: 1754 4226 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 3338 4883 1132 Size: 97 2109 1545 2229 Fragments arranged by size: 2229 2109 1545 97 PasI CC'CwG_GG Cuts at: 1348 3017 1348 Size: 1669 4311 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5980 PfoI T'CCnGG_A Cuts at: 2702 3223 2702 Size: 521 5459 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PspOMI G'GGCC_C Cuts at: 2292 2603 2292 Size: 311 5669 PstI C_TGCA'G Cuts at: 830 2730 3332 830 Size: 1900 602 3478 Fragments arranged by size: 3478 1900 602 PvuI CG_AT'CG Cuts at: 2001 2920 2001 Size: 919 5061 PvuII CAG'CTG Cuts at: 2564 2650 2564 Size: 86 5894 SacI G_AGCT'C Cuts at: 2877 2877 Size: 5980 SacII CC_GC'GG Cuts at: 2325 2490 2325 Size: 165 5815 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SapI GCTCTTCn'nnn_ Cuts at: 2865 2865 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2479 2726 3328 4463 5144 5335 826 Size: 219 1434 247 602 1135 681 191 1471 Fragments arranged by size: 1471 1434 1135 681 602 247 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 2340 2769 3307 1872 Size: 468 429 538 4545 Fragments arranged by size: 4545 538 468 429 SmlI C'TyrA_G Cuts at: 820 1017 1943 4084 4985 5247 820 Size: 197 926 2141 901 262 1553 Fragments arranged by size: 2141 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3277 3771 493 Size: 2784 494 2702 Fragments arranged by size: 2784 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 3419 513 Size: 552 2354 3074 Fragments arranged by size: 3074 2354 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2180 2952 3066 4007 4024 4177 4362 Size: 1255 600 772 114 941 17 153 185 Cuts at: 4362 4807 325 Size: 445 1498 Fragments arranged by size: 1498 1255 941 772 600 445 185 153 114 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4226 5938 96 Size: 274 80 33 51 1443 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2948 2980 5940 5972 2948 Size: 32 2960 32 2956 Fragments arranged by size: 2960 2956 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2381 1316 Size: 1065 4915 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 2338 2767 3305 1870 Size: 468 429 538 4545 Fragments arranged by size: 4545 538 468 429 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI